##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551846_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626623
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.208157057752427	28.0	25.0	31.0	16.0	33.0
2	27.754843023636223	31.0	25.0	33.0	16.0	34.0
3	27.57040517184974	31.0	25.0	33.0	16.0	34.0
4	31.798223174061597	35.0	28.0	37.0	19.0	37.0
5	33.16938893082443	35.0	32.0	35.0	28.0	37.0
6	32.81722183833023	35.0	32.0	35.0	28.0	37.0
7	34.09837015238828	35.0	33.0	37.0	31.0	37.0
8	34.12994256514683	35.0	35.0	37.0	31.0	37.0
9	36.44420967631255	39.0	37.0	39.0	32.0	39.0
10	35.41033603937296	37.0	34.0	39.0	31.0	39.0
11	35.90865320934597	37.0	35.0	39.0	31.0	39.0
12	35.92837000876125	37.0	35.0	39.0	31.0	39.0
13	35.99467303306773	37.0	35.0	39.0	31.0	39.0
14	36.86508155621482	39.0	36.0	40.0	31.0	41.0
15	37.12284100647439	39.0	36.0	40.0	32.0	41.0
16	36.69472394087035	38.0	35.0	40.0	32.0	41.0
17	36.87976981374766	38.0	35.0	40.0	32.0	41.0
18	36.692528043177475	38.0	35.0	40.0	31.0	41.0
19	36.30764430925772	38.0	34.0	40.0	30.0	41.0
20	36.746104116829414	38.0	35.0	40.0	32.0	41.0
21	36.80321820297053	39.0	35.0	40.0	32.0	41.0
22	37.19451887338958	39.0	35.0	40.0	32.0	41.0
23	37.5467625669661	39.0	36.0	40.0	33.0	41.0
24	37.61642008033539	39.0	36.0	41.0	33.0	41.0
25	36.555511048908194	38.0	35.0	40.0	31.0	41.0
26	36.87673609171703	39.0	35.0	40.0	32.0	41.0
27	37.18240792310528	39.0	35.0	40.0	32.0	41.0
28	37.2547544536348	39.0	35.0	40.0	32.0	41.0
29	37.366981422641686	39.0	35.0	41.0	33.0	41.0
30	36.95298608573257	39.0	35.0	40.0	32.0	41.0
31	37.12627848004303	39.0	35.0	40.0	32.0	41.0
32	37.07226833359133	39.0	35.0	40.0	32.0	41.0
33	37.1830430737461	39.0	35.0	41.0	33.0	41.0
34	37.23779369732678	40.0	35.0	41.0	33.0	41.0
35	37.157662581807564	40.0	35.0	41.0	33.0	41.0
36	37.023768677498275	40.0	35.0	41.0	32.0	41.0
37	37.075152045169105	40.0	35.0	41.0	32.0	41.0
38	36.96304955292098	39.0	35.0	41.0	32.0	41.0
39	36.86970794241514	39.0	35.0	41.0	32.0	41.0
40	36.75234072161411	39.0	35.0	41.0	31.0	41.0
41	36.611238336288324	39.0	35.0	40.0	31.0	41.0
42	36.74883462624258	39.0	35.0	41.0	32.0	41.0
43	36.6809054247929	39.0	35.0	41.0	31.0	41.0
44	36.72704800174906	39.0	35.0	41.0	32.0	41.0
45	36.707511534048386	39.0	35.0	41.0	32.0	41.0
46	36.551154362351845	39.0	35.0	40.0	31.0	41.0
47	36.48791697719362	39.0	35.0	40.0	31.0	41.0
48	36.48946336154275	39.0	35.0	40.0	31.0	41.0
49	36.46805335903725	39.0	35.0	40.0	31.0	41.0
50	36.358236132411356	39.0	35.0	40.0	31.0	41.0
51	35.43061936762615	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	15.0
10	25.0
11	31.0
12	37.0
13	50.0
14	42.0
15	86.0
16	103.0
17	167.0
18	332.0
19	583.0
20	1057.0
21	1496.0
22	2024.0
23	2639.0
24	3493.0
25	4527.0
26	5532.0
27	6179.0
28	7003.0
29	8572.0
30	11238.0
31	15954.0
32	23230.0
33	37055.0
34	51265.0
35	51829.0
36	76048.0
37	121235.0
38	148527.0
39	46241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.09174256291263	20.210557224998123	23.553875296629712	12.143824915459534
2	33.96555823836661	24.214878802725085	24.01156676342873	17.80799619547958
3	33.90268151663759	23.031711252220237	24.923279228499432	18.14232800264274
4	29.758243792519583	24.35818666087903	26.37486973826368	19.50869980833771
5	27.8874219426992	28.943240193864572	23.255929003563548	19.913408859872682
6	27.1731168501635	34.803701747302604	23.29295286001312	14.730228542520782
7	77.77499389585125	6.359485687566527	11.275200559187901	4.590319857394318
8	78.1699682265094	7.959809965481638	8.8802996379003	4.989922170108662
9	70.96579602089295	9.435657484643876	10.557384583713015	9.041161910750164
10	42.603926124639536	25.353043217373127	18.256112526989913	13.786918130997424
11	34.049819428907014	25.430282642035163	23.266461652381096	17.253436276676727
12	30.863533576009814	22.818824077635195	26.425937126469982	19.891705219885004
13	25.8452051712114	25.971437371433858	28.586566404361154	19.596791052993588
14	20.92486231753383	29.23129218046577	27.75959388659529	22.084251615405115
15	20.82288712670936	26.435193090582377	32.122983037647835	20.618936745060427
16	23.219383903878406	25.077119735470927	30.45212192977277	21.251374430877895
17	23.287846121192487	26.03239906610514	27.919817817092575	22.7599369956098
18	22.85999715937653	26.494718514960354	28.65423069373451	21.991053631928608
19	25.618593636045915	26.959431747637737	26.074848832551627	21.34712578376472
20	27.64564977665997	26.203155645419972	28.614015125522048	17.537179452398014
21	25.498585273761098	27.774116175116458	27.210779048965644	19.516519502156797
22	23.897941824669697	25.193457629228423	28.55608555702552	22.352514989076365
23	24.58336192575121	27.23982362600798	28.172122631949353	20.004691816291455
24	23.500254539013092	25.38879038911754	29.018245420292583	22.092709651576786
25	22.616629137455853	28.634920837569	27.261048509231227	21.48740151574392
26	22.113455778035597	28.042539134375854	28.148503964903938	21.69550112268461
27	23.029317468398062	28.990477527955406	27.790713076283506	20.189491927363022
28	20.652609304158958	27.86412244683007	30.418768541850522	21.064499707160444
29	23.01192263929029	26.81756015977709	28.812380011585915	21.358137189346703
30	21.630709373897865	29.510726545307147	27.509044513208103	21.349519567586892
31	23.808893066484952	27.414250673850145	26.67233727456541	22.104518985099496
32	24.822421136791977	28.56167105261058	26.56589368727289	20.05001412332455
33	23.462432754622796	28.512199520285726	25.92116791116828	22.104199813923202
34	23.327423347052374	26.70425439219435	27.009222451138882	22.959099809614393
35	23.400353960834504	27.16306295811038	26.688455418968022	22.748127662087093
36	23.759900290924527	28.82418934510862	26.415244892064287	21.000665471902565
37	21.750717736182683	29.112560502886105	28.48602748382999	20.650694277101223
38	22.0591966780664	28.273938237185675	26.558073993453803	23.10879109129413
39	22.78164063559748	27.177106489867114	27.541121216425186	22.50013165811022
40	23.20853208388457	26.87533014268547	27.75289129189321	22.163246481536746
41	21.563683426877088	26.71606372571706	26.858733241518422	24.86151960588743
42	22.41156165668991	26.180334906315277	27.433720115603798	23.974383321391013
43	23.981245501681233	26.231083123345293	27.383610240926366	22.404061134047108
44	22.111221579801573	27.042256667884836	27.266314833640003	23.58020691867359
45	21.175730862097307	27.103537533732403	27.555962676122647	24.164768928047646
46	22.365122250539798	29.00037183442038	26.334494584463066	22.30001133057676
47	21.156421005931797	27.108325101376746	29.79016091014853	21.94509298254293
48	21.88636548610568	26.32013188153004	28.527998493512047	23.26550413885223
49	21.692468996509863	25.62784960015831	29.51966333824325	23.16001806508858
50	21.386383838448317	26.598927904018844	28.676732261662913	23.337955995869926
51	21.188338123560737	26.67345437368242	26.969006882926415	25.169200619830423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	698.5
2	1130.0
3	2148.5
4	3167.0
5	2396.0
6	1625.0
7	1630.0
8	1635.0
9	1638.5
10	1642.0
11	1611.5
12	1581.0
13	1600.0
14	1619.0
15	1625.5
16	1632.0
17	1696.0
18	1760.0
19	1782.0
20	1804.0
21	1935.5
22	2067.0
23	2577.0
24	3087.0
25	3579.0
26	5592.0
27	7113.0
28	7774.5
29	8436.0
30	9302.5
31	10169.0
32	12334.0
33	14499.0
34	16696.5
35	18894.0
36	19453.0
37	20012.0
38	21162.5
39	22313.0
40	25603.5
41	28894.0
42	32132.5
43	35371.0
44	38341.0
45	41311.0
46	50203.0
47	59095.0
48	54126.5
49	49158.0
50	49109.5
51	49061.0
52	45177.0
53	41293.0
54	38841.5
55	36390.0
56	34343.5
57	32297.0
58	31106.0
59	29915.0
60	29294.5
61	28674.0
62	25992.5
63	23311.0
64	19453.0
65	15595.0
66	12912.5
67	10230.0
68	8360.0
69	6490.0
70	5345.0
71	4200.0
72	3638.5
73	3077.0
74	2492.0
75	1327.5
76	748.0
77	684.0
78	620.0
79	433.5
80	247.0
81	188.5
82	130.0
83	87.0
84	44.0
85	29.5
86	15.0
87	18.5
88	22.0
89	12.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.711313463081922
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7891972636139	17.733500999698176
2	9.30829942735189	4.414240110603332
3	3.0304321054200294	2.1556657674060493
4	1.4782819447511661	1.40208026315237
5	0.9020851082901737	1.0694811386523255
6	0.6342806009230879	0.9023775691223584
7	0.4949162596426838	0.8214580199254603
8	0.41783901096823695	0.7926009412937594
9	0.3675579973354818	0.7843754601615814
>10	7.141850790958161	44.21081005061092
>50	1.3655604207089924	20.1707527806349
>100	0.061578790031971406	2.3467722267529436
>500	0.003383450001756671	0.5452161921582458
>1k	0.004736830002459339	2.65066847982761
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	4031	0.6432895058113092	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	3726	0.5946159014271739	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	3593	0.573391018203928	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.23586749927787523	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	1435	0.2290053189876529	No Hit
GCCAGACAAAGGTTGCCAATCTTTGTGGTATAAACCAGAAGCTAATGGCTG	1138	0.18160839930867523	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTACCGT	1119	0.17857627313392582	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	801	0.12782805610390938	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	783	0.12495551551730466	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	685	0.1093161278791235	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5958558814470583E-4	0.0	0.0	0.06878138849036822	0.0
2	1.5958558814470583E-4	0.0	0.0	0.46184069209077866	0.0
3	1.5958558814470583E-4	0.0	0.0	0.5875941355488069	0.0
4	1.5958558814470583E-4	0.0	0.0	0.8884129692015773	0.0
5	1.5958558814470583E-4	0.0	0.0	1.9542851124200675	0.0
6	1.5958558814470583E-4	0.0	0.0	2.2260593690305015	0.0
7	1.5958558814470583E-4	0.0	0.0	2.5999684020535474	0.0
8	1.5958558814470583E-4	0.0	0.0	3.006592480646258	0.0
9	1.5958558814470583E-4	0.0	0.0	3.1423998161574023	0.0
10	1.5958558814470583E-4	0.0	0.0	4.134064660888605	0.0
11	1.5958558814470583E-4	0.0	0.0	4.724850508200306	0.0
12	1.5958558814470583E-4	0.0	0.0	5.764550614963064	0.0
13	1.5958558814470583E-4	0.0	0.0	5.991002564540402	0.0
14	1.5958558814470583E-4	0.0	0.0	6.0875518453679485	0.0
15	1.5958558814470583E-4	0.0	0.0	6.364432840799013	0.0
16	1.5958558814470583E-4	0.0	0.0	6.719670360009128	0.0
17	1.5958558814470583E-4	0.0	0.0	7.111772150080671	0.0
18	1.5958558814470583E-4	0.0	0.0	7.481212786635664	3.1917117628941165E-4
19	1.5958558814470583E-4	0.0	0.0	7.904114595219135	3.1917117628941165E-4
20	1.5958558814470583E-4	0.0	0.0	8.176367608594003	3.1917117628941165E-4
21	1.5958558814470583E-4	0.0	0.0	8.490272460474639	3.1917117628941165E-4
22	1.5958558814470583E-4	0.0	0.0	8.850616718505385	3.1917117628941165E-4
23	1.5958558814470583E-4	0.0	0.0	9.165957840679324	3.1917117628941165E-4
24	1.5958558814470583E-4	0.0	0.0	9.42496525023818	3.1917117628941165E-4
25	1.5958558814470583E-4	0.0	0.0	9.678067993035684	3.1917117628941165E-4
26	1.5958558814470583E-4	0.0	0.0	9.907392483199628	3.1917117628941165E-4
27	1.5958558814470583E-4	0.0	0.0	10.148685892474422	3.1917117628941165E-4
28	1.5958558814470583E-4	0.0	0.0	10.38567049086931	3.1917117628941165E-4
29	1.5958558814470583E-4	0.0	0.0	10.620420891030173	3.1917117628941165E-4
30	1.5958558814470583E-4	0.0	0.0	10.88756716558441	3.1917117628941165E-4
31	1.5958558814470583E-4	0.0	0.0	11.163969404251041	3.1917117628941165E-4
32	1.5958558814470583E-4	0.0	0.0	11.424253498515055	3.1917117628941165E-4
33	1.5958558814470583E-4	0.0	0.0	11.678632926017718	3.1917117628941165E-4
34	1.5958558814470583E-4	0.0	0.0	11.943225831161639	3.1917117628941165E-4
35	1.5958558814470583E-4	0.0	0.0	12.22569232217777	3.1917117628941165E-4
36	1.5958558814470583E-4	0.0	0.0	12.49060439849798	3.1917117628941165E-4
37	1.5958558814470583E-4	0.0	0.0	12.780252240980621	3.1917117628941165E-4
38	1.5958558814470583E-4	0.0	0.0	13.058090749940554	3.1917117628941165E-4
39	1.5958558814470583E-4	0.0	0.0	13.3566753853593	3.1917117628941165E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAT	20	7.0308277E-4	45.0	43
GGCGTAT	20	7.0308277E-4	45.0	25
GTAATCG	20	7.0308277E-4	45.0	34
TGGCACG	20	7.0308277E-4	45.0	2
ATATACG	20	7.0308277E-4	45.0	1
TCGTCGT	20	7.0308277E-4	45.0	29
GTCGTAA	20	7.0308277E-4	45.0	31
AACGAGC	40	6.8066583E-9	45.0	15
ATACGCA	20	7.0308277E-4	45.0	38
CCCGCCA	20	7.0308277E-4	45.0	40
TCGCCGT	25	3.8887687E-5	44.999996	13
TACGAAT	90	0.0	44.999996	12
CGTATGG	60	3.6379788E-12	41.249996	2
GGCCGAT	115	0.0	41.086956	8
TACGGGA	155	0.0	40.64516	4
CTAGCGG	50	1.0804797E-9	40.499996	2
CGAATAT	95	0.0	40.263153	14
CGTAAGG	90	0.0	39.999996	2
CCGATAA	80	0.0	39.375	11
ACGGGAC	160	0.0	39.375	5
>>END_MODULE