FastQCFastQC Report
Sat 18 Jun 2016
SRR3551844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15710.22591184405036496No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG10890.15659961691333382No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC10290.14797153884648348No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC9920.1426508907052591No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC9820.1412128776941174No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGC253.8892103E-545.00000418
TTAAGCG302.164259E-645.0000041
CTCGATG302.164259E-645.0000041
ATTATCG253.8892103E-545.00000417
TGCGTCG253.8892103E-545.0000041
TCGTTTA351.2110104E-745.011
TAGAACG207.0313626E-445.01
TACGGTA207.0313626E-445.01
AGACGTC207.0313626E-445.019
CCGTATT207.0313626E-445.023
ACGGATC207.0313626E-445.029
GACGCAC207.0313626E-445.026
TATCGCG207.0313626E-445.01
CGGAGAT207.0313626E-445.029
GTACGAT207.0313626E-445.010
TTCGACG603.6379788E-1241.25000425
ATGCGCG556.002665E-1140.9090921
TAGTACG556.002665E-1140.9090921
GATAGCG451.9264917E-840.01
TGCGTAG451.9264917E-840.01