##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551844_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695404
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.171149432560068	28.0	25.0	31.0	16.0	33.0
2	27.654784844493275	31.0	25.0	33.0	16.0	34.0
3	27.518051377328863	31.0	25.0	33.0	16.0	34.0
4	31.63330668215886	35.0	28.0	37.0	19.0	37.0
5	33.14287234470898	35.0	32.0	35.0	28.0	37.0
6	32.839158244703796	35.0	32.0	35.0	28.0	37.0
7	34.22623539697787	35.0	33.0	37.0	32.0	37.0
8	34.35055018377806	35.0	35.0	37.0	32.0	37.0
9	36.671901513364894	39.0	37.0	39.0	32.0	39.0
10	35.52033925602959	37.0	34.0	39.0	31.0	39.0
11	36.02157594721917	37.0	35.0	39.0	31.0	39.0
12	36.000550758983266	37.0	35.0	39.0	31.0	39.0
13	36.19783751603384	37.0	35.0	39.0	32.0	39.0
14	37.053888099579524	39.0	36.0	40.0	32.0	41.0
15	37.31634848232107	39.0	36.0	40.0	32.0	41.0
16	36.74520710263386	38.0	35.0	40.0	31.0	41.0
17	37.05774053643638	38.0	35.0	40.0	32.0	41.0
18	36.812539185854554	38.0	35.0	40.0	32.0	41.0
19	36.29580934248293	38.0	34.0	40.0	30.0	41.0
20	36.98709958527705	38.0	35.0	40.0	32.0	41.0
21	37.00148546744051	39.0	35.0	40.0	32.0	41.0
22	37.432383190203105	39.0	36.0	40.0	33.0	41.0
23	37.78007604212803	39.0	36.0	40.0	34.0	41.0
24	37.852087419686974	40.0	36.0	41.0	34.0	41.0
25	36.70325882508585	39.0	35.0	40.0	31.0	41.0
26	37.08813150341384	39.0	35.0	40.0	32.0	41.0
27	37.43899229800231	39.0	35.0	40.0	33.0	41.0
28	37.53081805684178	39.0	36.0	41.0	33.0	41.0
29	37.653349707508156	40.0	36.0	41.0	33.0	41.0
30	37.21719604718984	39.0	35.0	41.0	32.0	41.0
31	37.32862192337116	39.0	35.0	40.0	33.0	41.0
32	37.30822802284715	39.0	35.0	40.0	33.0	41.0
33	37.46907409218239	39.0	35.0	41.0	33.0	41.0
34	37.52447354343662	40.0	36.0	41.0	33.0	41.0
35	37.45025625391859	40.0	35.0	41.0	33.0	41.0
36	37.34113119855508	40.0	35.0	41.0	33.0	41.0
37	37.386552277525006	40.0	35.0	41.0	33.0	41.0
38	37.24010503247033	39.0	35.0	41.0	33.0	41.0
39	37.16198928967909	39.0	35.0	41.0	33.0	41.0
40	37.03101937866334	39.0	35.0	41.0	32.0	41.0
41	36.887765960506414	39.0	35.0	41.0	32.0	41.0
42	37.0058728451375	39.0	35.0	41.0	33.0	41.0
43	36.966567635503964	39.0	35.0	41.0	33.0	41.0
44	36.972926529039235	39.0	35.0	41.0	33.0	41.0
45	36.94009093994283	39.0	35.0	41.0	33.0	41.0
46	36.77182903750913	39.0	35.0	40.0	32.0	41.0
47	36.76277818361701	39.0	35.0	41.0	32.0	41.0
48	36.72477725178457	39.0	35.0	40.0	32.0	41.0
49	36.6727801393147	39.0	35.0	40.0	32.0	41.0
50	36.57112987558311	38.0	35.0	40.0	32.0	41.0
51	35.6188302051757	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	20.0
10	24.0
11	24.0
12	39.0
13	39.0
14	64.0
15	79.0
16	112.0
17	176.0
18	276.0
19	438.0
20	642.0
21	1087.0
22	1470.0
23	2111.0
24	2996.0
25	3990.0
26	5249.0
27	6196.0
28	7100.0
29	8746.0
30	11695.0
31	16337.0
32	24609.0
33	40652.0
34	57059.0
35	57765.0
36	85313.0
37	138095.0
38	169569.0
39	53424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69733852551898	20.662233751890987	24.084417115806065	12.556010606783971
2	33.820340406439996	22.88223823849158	25.472099671557828	17.82532168351059
3	35.06997371312215	21.06286417679507	25.562263087356413	18.304899022726357
4	29.80814030405347	25.681905769883407	24.581969617661102	19.92798430840202
5	26.658604207050868	29.89456488602309	23.189397817671452	20.257433089254594
6	27.211664011135973	34.04840927000707	23.049479151687365	15.690447567169588
7	81.16346756705455	6.181442729693818	7.95364996462488	4.701439738626755
8	82.38448441481498	5.576758258508723	7.46256852131998	4.576188805356311
9	76.01854461579168	7.920431864067506	9.640295425393008	6.420728094747802
10	43.143266360274026	23.77725753662619	17.52434556027863	15.555130542821152
11	33.13584621313654	24.78674267044768	23.074212975479004	19.003198140936778
12	29.928070589182692	23.072631161166747	27.715399968938918	19.283898280711647
13	25.183490460221687	25.67514710873104	29.365663700525165	19.775698730522116
14	22.190985384035756	27.80024273659628	28.474958441423976	21.533813437943987
15	21.283886776607556	25.77408240389759	32.83731471202351	20.10471610747134
16	24.347142092941656	24.092326187367345	30.31647790349207	21.244053816198928
17	23.962473612461245	24.532214367475596	27.59518208120747	23.910129938855686
18	25.56758373549764	24.264024940897666	28.880909514469288	21.287481809135407
19	25.636752161333554	25.672558685310985	27.337202547008644	21.353486606346813
20	27.228057359462987	25.20017141115093	27.168092216898376	20.403679012487704
21	25.8228310449753	26.40623292359549	26.228925919321718	21.542010112107494
22	25.150128558363193	24.86223835353262	26.65745379664195	23.33017929146223
23	23.905096893316692	26.545432583074014	27.04442309794019	22.505047425669108
24	23.932850544431723	25.204916854087696	28.975530770602415	21.886701830878167
25	22.371887420837382	26.50804424478433	28.241568929715676	22.878499404662612
26	22.340970141097838	27.296362977492223	27.769613059458965	22.59305382195098
27	23.33061069536557	27.588711022657332	27.645081132694088	21.435597149283005
28	21.305169369172454	27.602515947564292	29.02471081558346	22.067603867679793
29	22.648273521578822	26.933552294781165	28.219567330645205	22.198606852994807
30	23.578667939787522	27.753938717637517	26.027316495159646	22.640076847415315
31	25.83189052694549	26.550609429914125	24.504316915059448	23.113183128080944
32	25.779115449436585	27.63832247154172	25.25237128345537	21.330190795566317
33	24.250795221195162	28.72387849365261	24.55694819126723	22.468378093884994
34	23.820397926960442	27.156156708905904	26.02544707824516	22.997998285888492
35	24.42134356431657	26.498409557609676	26.38681974794508	22.693427130128676
36	24.899770493123423	28.945763901271775	24.80529303829141	21.34917256731339
37	24.22318537138124	29.66678362505824	26.183053304266295	19.926977699294223
38	23.60009433365353	28.926638328223593	25.090594819701927	22.382672518420947
39	23.66681813737051	28.006741404996234	27.012211606490617	21.314228851142644
40	24.66753139182403	27.15083606076468	26.911838298312922	21.269794249098368
41	21.982042093516863	27.645368735296316	26.392571799989646	23.980017371197174
42	22.836940828640618	27.266883710763818	26.3789106763838	23.51726478421177
43	23.633743838114246	26.017106602780544	26.60367211002525	23.74547744907996
44	22.515401119349328	26.735394101845834	26.667951291623286	24.081253487181552
45	22.82903175707934	27.266308505559355	26.203041685121164	23.701618052240136
46	23.538978780680008	28.160896399790623	26.295793524339807	22.00433129518956
47	23.348873460607074	26.9085308683873	27.611863032136714	22.130732638868917
48	23.61735048978723	25.62381004423328	28.23725489068225	22.521584575297236
49	22.7850285589384	25.886966425272217	28.733800783429487	22.594204232359893
50	21.873472111175662	27.07979821801428	28.3026844826892	22.74404518812086
51	21.916612501509913	27.308010882882467	26.42233866932028	24.353037946287337
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	191.0
1	735.0
2	1279.0
3	2274.0
4	3269.0
5	2406.5
6	1544.0
7	1615.5
8	1687.0
9	1641.0
10	1595.0
11	1732.5
12	1870.0
13	1741.5
14	1613.0
15	1558.5
16	1504.0
17	1461.5
18	1419.0
19	1543.0
20	1667.0
21	1779.5
22	1892.0
23	2185.5
24	2479.0
25	2934.0
26	4665.5
27	5942.0
28	7164.0
29	8386.0
30	10039.0
31	11692.0
32	12978.0
33	14264.0
34	15699.5
35	17135.0
36	18681.0
37	20227.0
38	21803.5
39	23380.0
40	26249.0
41	29118.0
42	33939.0
43	38760.0
44	41558.0
45	44356.0
46	49326.0
47	54296.0
48	55923.5
49	57551.0
50	55826.5
51	54102.0
52	51242.0
53	48382.0
54	46808.0
55	45234.0
56	42063.5
57	38893.0
58	37969.5
59	37046.0
60	34981.5
61	32917.0
62	29899.0
63	26881.0
64	24194.0
65	21507.0
66	18036.5
67	14566.0
68	11990.0
69	9414.0
70	7760.5
71	6107.0
72	5305.0
73	4503.0
74	3493.5
75	1966.0
76	1448.0
77	1104.5
78	761.0
79	578.0
80	395.0
81	249.5
82	104.0
83	101.0
84	98.0
85	65.0
86	32.0
87	25.0
88	18.0
89	12.5
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.940989245343516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21811887903903	17.768551858927
2	9.893506994139626	4.737206890908553
3	3.330606066433857	2.3921401205090653
4	1.6516216345668011	1.5816582316216188
5	0.9237784032169085	1.105808440824831
6	0.6575192245781529	0.9444996410539301
7	0.49037493787061903	0.821804277962254
8	0.3481162445833101	0.6667397814158721
9	0.3190817744139351	0.6875219996666232
>10	6.556596259460118	41.8817770876945
>50	1.5292407264432883	23.349494378777326
>100	0.07661284901707993	2.9871258171509862
>500	0.003016253898310233	0.5428905838935627
>1k	0.00180975233898614	0.5327808895938688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1571	0.22591184405036496	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	1089	0.15659961691333382	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	1029	0.14797153884648348	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	992	0.1426508907052591	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	982	0.1412128776941174	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06643620111474768	0.0
2	0.0	0.0	0.0	0.39113953903054915	0.0
3	0.0	0.0	0.0	0.5135144462787099	0.0
4	0.0	0.0	0.0	0.749492381407067	0.0
5	0.0	0.0	0.0	1.416442815974599	0.0
6	0.0	0.0	0.0	1.7512122449683925	0.0
7	0.0	0.0	0.0	2.1295534681997803	0.0
8	0.0	0.0	0.0	2.7675998412433636	0.0
9	0.0	0.0	0.0	3.0309000235834134	0.0
10	0.0	0.0	0.0	3.745880092723079	0.0
11	0.0	0.0	0.0	4.536643447549913	0.0
12	0.0	0.0	0.0	5.268735871522166	0.0
13	0.0	0.0	0.0	5.501693979327125	0.0
14	0.0	0.0	0.0	5.591713593824597	0.0
15	0.0	0.0	0.0	5.809716366313682	0.0
16	0.0	0.0	0.0	6.217393054972361	0.0
17	0.0	0.0	0.0	6.691505944745788	0.0
18	0.0	0.0	0.0	7.2307608239239345	0.0
19	0.0	0.0	0.0	7.558627790464248	0.0
20	0.0	0.0	0.0	7.858165900685069	0.0
21	0.0	0.0	0.0	8.227016238042921	0.0
22	1.4380130111417248E-4	0.0	0.0	8.623045021311352	0.0
23	1.4380130111417248E-4	0.0	0.0	9.004837475769481	0.0
24	1.4380130111417248E-4	0.0	0.0	9.323069755135144	0.0
25	1.4380130111417248E-4	0.0	0.0	9.610528556062375	0.0
26	1.4380130111417248E-4	0.0	0.0	9.880299796952563	0.0
27	1.4380130111417248E-4	0.0	0.0	10.161143738028542	0.0
28	1.4380130111417248E-4	0.0	0.0	10.431346382822072	0.0
29	1.4380130111417248E-4	0.0	0.0	10.701692828916716	0.0
30	1.4380130111417248E-4	0.0	0.0	11.011440831516644	0.0
31	1.4380130111417248E-4	0.0	0.0	11.342759029283696	0.0
32	1.4380130111417248E-4	0.0	0.0	11.655383057905908	0.0
33	1.4380130111417248E-4	0.0	0.0	11.942410454929796	0.0
34	1.4380130111417248E-4	0.0	0.0	12.24928243150744	0.0
35	1.4380130111417248E-4	0.0	0.0	12.572403955110985	0.0
36	1.4380130111417248E-4	0.0	0.0	12.8992643125435	0.0
37	1.4380130111417248E-4	0.0	0.0	13.216921386704707	0.0
38	1.4380130111417248E-4	0.0	0.0	13.52062973465784	0.0
39	1.4380130111417248E-4	0.0	0.0	13.821605857889802	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCGC	25	3.8892103E-5	45.000004	18
TTAAGCG	30	2.164259E-6	45.000004	1
CTCGATG	30	2.164259E-6	45.000004	1
ATTATCG	25	3.8892103E-5	45.000004	17
TGCGTCG	25	3.8892103E-5	45.000004	1
TCGTTTA	35	1.2110104E-7	45.0	11
TAGAACG	20	7.0313626E-4	45.0	1
TACGGTA	20	7.0313626E-4	45.0	1
AGACGTC	20	7.0313626E-4	45.0	19
CCGTATT	20	7.0313626E-4	45.0	23
ACGGATC	20	7.0313626E-4	45.0	29
GACGCAC	20	7.0313626E-4	45.0	26
TATCGCG	20	7.0313626E-4	45.0	1
CGGAGAT	20	7.0313626E-4	45.0	29
GTACGAT	20	7.0313626E-4	45.0	10
TTCGACG	60	3.6379788E-12	41.250004	25
ATGCGCG	55	6.002665E-11	40.909092	1
TAGTACG	55	6.002665E-11	40.909092	1
GATAGCG	45	1.9264917E-8	40.0	1
TGCGTAG	45	1.9264917E-8	40.0	1
>>END_MODULE