##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551841_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	314248
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.33510475802551	28.0	25.0	31.0	16.0	33.0
2	27.556105369007916	31.0	25.0	33.0	16.0	34.0
3	27.470131870369897	30.0	25.0	33.0	16.0	34.0
4	31.772078103917924	35.0	28.0	37.0	19.0	37.0
5	33.175673353529696	35.0	32.0	35.0	28.0	37.0
6	32.87415035258776	35.0	32.0	35.0	28.0	37.0
7	34.09473091316413	35.0	33.0	37.0	31.0	37.0
8	34.140669153025634	35.0	35.0	37.0	31.0	37.0
9	36.41478068277284	39.0	37.0	39.0	32.0	39.0
10	35.37623469361778	37.0	34.0	39.0	31.0	39.0
11	35.8793818894631	37.0	35.0	39.0	31.0	39.0
12	35.808345637840176	37.0	35.0	39.0	31.0	39.0
13	35.7972843104809	37.0	35.0	39.0	31.0	39.0
14	36.596710241592625	38.0	35.0	40.0	31.0	41.0
15	36.9339788956493	39.0	35.0	40.0	32.0	41.0
16	36.64766999312645	38.0	35.0	40.0	32.0	41.0
17	36.73623062040172	38.0	35.0	40.0	32.0	41.0
18	36.54624373106591	38.0	35.0	40.0	31.0	41.0
19	36.12687431582699	38.0	34.0	40.0	30.0	41.0
20	36.44878567246251	38.0	35.0	40.0	31.0	41.0
21	36.60964270257885	38.0	35.0	40.0	31.0	41.0
22	37.00159746442301	39.0	35.0	40.0	32.0	41.0
23	37.33962666429063	39.0	35.0	40.0	33.0	41.0
24	37.427404470354624	39.0	35.0	41.0	33.0	41.0
25	36.43313561263715	38.0	35.0	40.0	31.0	41.0
26	36.657773478271935	38.0	35.0	40.0	31.0	41.0
27	36.892909421857894	39.0	35.0	40.0	32.0	41.0
28	36.94640538682824	39.0	35.0	40.0	32.0	41.0
29	37.08192574017973	39.0	35.0	40.0	32.0	41.0
30	36.712615513861664	39.0	35.0	40.0	31.0	41.0
31	36.84195285252412	39.0	35.0	40.0	32.0	41.0
32	36.749178992388174	39.0	35.0	40.0	31.0	41.0
33	36.83201165958097	39.0	35.0	40.0	32.0	41.0
34	36.83192574017973	39.0	35.0	41.0	32.0	41.0
35	36.79316017922151	39.0	35.0	41.0	32.0	41.0
36	36.76118225096103	39.0	35.0	41.0	31.0	41.0
37	36.800355133525116	39.0	35.0	41.0	32.0	41.0
38	36.584566966217764	39.0	35.0	41.0	31.0	41.0
39	36.53923016216491	39.0	35.0	41.0	31.0	41.0
40	36.38094116748555	39.0	35.0	40.0	31.0	41.0
41	36.16844975942568	39.0	35.0	40.0	30.0	41.0
42	36.35218999007154	39.0	35.0	40.0	31.0	41.0
43	36.382637280109975	39.0	35.0	40.0	31.0	41.0
44	36.47345408721774	39.0	35.0	40.0	31.0	41.0
45	36.44202667956518	39.0	35.0	40.0	31.0	41.0
46	36.25059507140857	38.0	35.0	40.0	31.0	41.0
47	36.12642562562053	38.0	35.0	40.0	31.0	41.0
48	36.17647526794124	38.0	35.0	40.0	31.0	41.0
49	36.16920394083654	38.0	35.0	40.0	31.0	41.0
50	36.030030421832436	38.0	35.0	40.0	31.0	41.0
51	35.07174588223314	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	11.0
11	10.0
12	20.0
13	9.0
14	24.0
15	41.0
16	53.0
17	87.0
18	148.0
19	372.0
20	593.0
21	953.0
22	1279.0
23	1705.0
24	2102.0
25	2644.0
26	3005.0
27	3372.0
28	3689.0
29	4626.0
30	5961.0
31	8482.0
32	12685.0
33	20624.0
34	28332.0
35	26286.0
36	37440.0
37	58610.0
38	69656.0
39	21413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20442453094372	19.829879585550266	21.93585957587638	13.029836307629644
2	30.71109442223976	28.507420890506864	23.51391257860034	17.267572108653038
3	31.56328759451134	26.21623685751381	25.370726305338458	16.84974924263639
4	28.167880145617474	24.347330770601562	29.515223645019223	17.96956543876174
5	30.05842519284132	27.348463633817875	22.93061531020086	19.662495863139938
6	25.04582368065986	36.65512588783381	23.15464219342685	15.14440823807948
7	75.45569104656195	6.245067589928973	13.769061378274483	4.530179985234592
8	76.22164659759171	9.891550622438329	9.155825971843894	4.730976808126066
9	70.50482421526947	7.656373310251776	10.285506988111301	11.553295486367455
10	45.859321300374226	23.191555713958405	17.28921106896464	13.659911916702733
11	35.87771441663909	23.563873119319773	23.640882360428705	16.91753010361243
12	31.55628675441053	22.243260100302944	25.483694406965203	20.716758738321325
13	26.76421170540465	25.786958071332194	28.703762633334186	18.745067589928972
14	19.82765204551819	31.026768666785472	27.395560194496067	21.750019093200276
15	17.665028894376416	26.475586161248444	35.63173035309692	20.227654591278228
16	21.053753723174054	23.753850462055446	32.88708281357399	22.30531300119651
17	21.24564038593722	25.63262136910975	28.77472569435605	24.34701255059698
18	20.972607622005548	25.646304829306786	29.720157327970263	23.660930220717393
19	24.347330770601562	26.43549044067106	26.48736030141799	22.729818487309387
20	28.369631628522697	24.369606170922328	29.082126218782616	18.17863598177236
21	24.52139711310812	29.206868460578907	26.515045441816653	19.75668898449632
22	22.78773452814338	24.55799241363509	28.902013696188998	23.752259362032536
23	23.036264351722206	27.99285914309717	27.819747460604365	21.15112904457626
24	23.03658257172679	24.929673378987296	29.761207708561393	22.27253634072452
25	21.85280415468038	28.278938927216718	28.427547669356684	21.440709248746213
26	20.95510552175352	27.544168936636034	28.944973396807615	22.55575214480283
27	23.284794175301034	29.70838318780072	26.179959776991417	20.826862859906825
28	20.405221353835188	27.59190193732339	31.025814006771725	20.977062702069706
29	23.60237773987424	24.681461775413048	29.18968458033146	22.52647590438125
30	21.418433848425448	28.57870217153331	27.2542705124615	22.748593467579745
31	25.085601181232658	27.286410732924317	24.13857844759553	23.489409638247498
32	28.48164507013569	28.216567806318576	24.46093531223747	18.840851811308266
33	25.51742572745093	27.913622361956165	23.921870624474938	22.64708128611797
34	24.463162852269544	28.11250986482014	25.39363814566839	22.030689137241925
35	23.705799241363508	26.078765815534226	26.41798834041903	23.79744660268323
36	24.890532318423666	29.90217917059138	24.77660955678318	20.430678954201777
37	23.285748835314784	28.458096789796596	27.667956518418574	20.58819785647005
38	23.296250095466	28.764224434204834	24.37501591100023	23.56450955932894
39	25.312173824495304	28.384906188742647	25.41464066597083	20.888279320791224
40	24.693554135587178	25.435961406277848	28.04982052391742	21.82066393421756
41	20.73617015860085	28.73876683383824	26.042488735011837	24.48257427254907
42	21.29400982663374	25.829599551946238	29.286423461724496	23.589967159695526
43	23.804765662788625	26.83262900638986	25.85505715231282	23.507548178508696
44	23.171826073674296	26.266197398233242	27.256498052493573	23.30547847559889
45	22.57834576512818	26.55736870242611	26.445355260813113	24.418930271632597
46	25.462373666658184	28.00749726330796	25.342086504926048	21.18804256510781
47	21.547312950281306	27.113299050431504	30.112840813624906	21.226547185662277
48	23.361166976400803	25.887197372775645	27.228494692090322	23.52314095873323
49	21.843893994552076	24.558947073648838	30.448562918459306	23.148596013339784
50	21.472213029199867	26.322522339044323	28.05745780402739	24.14780682772842
51	20.544601715842266	25.986163794200756	26.478450141289684	26.990784348667297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	63.0
1	298.0
2	533.0
3	1321.5
4	2110.0
5	1527.0
6	944.0
7	944.0
8	944.0
9	981.5
10	1019.0
11	952.5
12	886.0
13	876.0
14	866.0
15	777.5
16	689.0
17	869.5
18	1050.0
19	974.0
20	898.0
21	928.5
22	959.0
23	1020.5
24	1082.0
25	1394.5
26	2211.0
27	2715.0
28	2877.5
29	3040.0
30	3718.0
31	4396.0
32	5089.5
33	5783.0
34	6369.5
35	6956.0
36	7705.0
37	8454.0
38	9129.0
39	9804.0
40	11367.5
41	12931.0
42	15608.5
43	18286.0
44	19633.5
45	20981.0
46	28959.5
47	36938.0
48	31113.5
49	25289.0
50	26214.0
51	27139.0
52	24777.0
53	22415.0
54	20599.5
55	18784.0
56	17636.0
57	16488.0
58	16023.5
59	15559.0
60	14933.0
61	14307.0
62	12956.5
63	11606.0
64	9138.0
65	6670.0
66	5601.5
67	4533.0
68	3740.0
69	2947.0
70	2444.0
71	1941.0
72	1498.5
73	1056.0
74	832.0
75	500.5
76	393.0
77	296.0
78	199.0
79	155.5
80	112.0
81	112.5
82	113.0
83	65.5
84	18.0
85	24.5
86	31.0
87	16.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	314248.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.140818716427788
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65948847797242	19.77800972480334
2	9.49152129718675	4.962322751457448
3	3.1175818958695993	2.444884295206334
4	1.4814904987400634	1.5490949823069677
5	0.8570002556392808	1.1201344161299356
6	0.48936662324856656	0.7675466510526718
7	0.44797740635689676	0.8197347318041802
8	0.39198023056228465	0.8197347318041802
9	0.29459383787600274	0.6930831699803977
>10	6.86939267410861	46.41907028843461
>50	0.8521309360049667	13.475344314044957
>100	0.03408523744019867	1.4924518214913063
>500	0.006086649542892619	1.0523535551538912
>1k	0.007303979451471143	4.6062345663297775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	4076	1.2970647386777323	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	3483	1.1083602759603879	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	3436	1.0934039357450167	No Hit
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1236	0.3933199256638069	RNA PCR Primer, Index 23 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	0.36531656526055856	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGGATAT	1096	0.3487691250222754	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCT	731	0.23261882334971104	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTT	723	0.2300730633130521	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGGATATCGTA	678	0.21575316310684556	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCT	649	0.20652478297395688	RNA PCR Primer, Index 23 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTC	526	0.1673837224103256	No Hit
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	344	0.10946768157633462	RNA PCR Primer, Index 1 (95% over 22bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	320	0.10183040146635779	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09387490135179857	0.0
2	0.0	0.0	0.0	0.714085690282834	0.0
3	0.0	0.0	0.0	0.873195692574018	0.0
4	0.0	0.0	0.0	1.3078842188335327	0.0
5	0.0	0.0	0.0	2.9642193426847587	0.0
6	0.0	0.0	0.0	3.482599730149436	0.0
7	0.0	0.0	0.0	4.0251648379623735	0.0
8	0.0	0.0	0.0	4.665423487182098	0.0
9	0.0	0.0	0.0	4.860810569995672	0.0
10	0.0	0.0	0.0	6.433772052646318	0.0
11	0.0	0.0	0.0	7.1869988034927825	0.0
12	0.0	0.0	0.0	9.090590870904508	0.0
13	0.0	0.0	0.0	9.415493495583107	0.0
14	0.0	0.0	0.0	9.584468318016343	0.0
15	0.0	0.0	0.0	10.009610244138388	0.0
16	0.0	0.0	0.0	10.440480130342914	0.0
17	0.0	0.0	0.0	10.93053893739976	0.0
18	0.0	0.0	0.0	11.400231664163336	0.0
19	0.0	0.0	0.0	12.078994933937526	0.0
20	0.0	0.0	0.0	12.43667421908811	0.0
21	0.0	0.0	0.0	12.800399684325756	0.0
22	0.0	0.0	0.0	13.237315750617347	0.0
23	0.0	0.0	0.0	13.636681856368218	0.0
24	0.0	0.0	0.0	13.961902701051399	0.0
25	0.0	0.0	0.0	14.259756625340495	0.0
26	0.0	0.0	0.0	14.56811180978081	0.0
27	0.0	0.0	0.0	14.862147094014919	0.0
28	0.0	0.0	0.0	15.125951477813702	0.0
29	0.0	0.0	0.0	15.404393981823274	0.0
30	0.0	0.0	0.0	15.737570326621013	0.0
31	0.0	0.0	0.0	16.06788269137751	0.0
32	0.0	0.0	0.0	16.38896667600112	0.0
33	0.0	0.0	0.0	16.678865100175656	0.0
34	0.0	0.0	0.0	16.982765204551818	0.0
35	0.0	0.0	0.0	17.288256408950893	0.0
36	0.0	0.0	0.0	17.62525139380362	0.0
37	0.0	0.0	0.0	17.97433873883048	0.0
38	0.0	0.0	0.0	18.26773758305542	0.0
39	0.0	0.0	0.0	18.57545632748657	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	2.16045E-6	45.000004	1
CGAACAC	30	2.16045E-6	45.000004	34
CGCGGGC	75	0.0	45.000004	4
GAGACCG	30	2.16045E-6	45.000004	10
ACGGATG	30	2.16045E-6	45.000004	37
AGACTCG	20	7.025454E-4	45.0	15
ACACGAC	25	3.884315E-5	45.0	26
GATCGGC	20	7.025454E-4	45.0	16
CGCGGAT	20	7.025454E-4	45.0	37
CCCTATT	35	1.2081546E-7	45.0	17
TAGCGCG	20	7.025454E-4	45.0	1
CGACCTT	20	7.025454E-4	45.0	37
TTCGTCG	20	7.025454E-4	45.0	1
CCGTCCA	20	7.025454E-4	45.0	17
TCGGCAT	20	7.025454E-4	45.0	18
CTGCCGT	20	7.025454E-4	45.0	14
AATCTTA	20	7.025454E-4	45.0	39
AGACACG	25	3.884315E-5	45.0	24
GGACGCA	35	1.2081546E-7	45.0	8
GCGATAT	20	7.025454E-4	45.0	9
>>END_MODULE