FastQCFastQC Report
Sat 18 Jun 2016
SRR3551838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591286
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG48010.8119590181401216No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC47420.8019807673443985No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC44530.753104250734839RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAGATGT17090.2890310272862879No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15070.25486820252804904No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC14020.23711029856955854RNA PCR Primer, Index 47 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11430.19330746880528205No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT9580.16201973325937025TruSeq Adapter, Index 9 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTA9540.1613432416799992No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT8780.14848990167194892No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC6830.11551093717760948No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT207.0305035E-445.00000420
GTATTCG253.8884988E-545.00000426
TTTACGG502.1827873E-1145.0000042
CGCATCG302.163706E-645.00000421
TTGTCGT207.0305035E-445.00000442
TAACGCG302.163706E-645.0000041
TATGCGA253.8884988E-545.00000420
TAGGCCG406.8048394E-945.0000041
AATAGCG253.8884988E-545.0000041
CGCGTAA207.0305035E-445.00000431
TACGGCT5200.042.4038437
GTCGAAT750.042.043
ACCCGTG650.041.5384639
CGTATGG603.6379788E-1241.2500042
GCTACGA1700.041.0294110
GCGTTAG556.002665E-1140.9090921
ACGTGCG556.002665E-1140.9090921
GTCGTTT556.002665E-1140.9090929
ACGGCTG5500.040.0909088
CGAATAT1750.039.85714314