Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551838_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 591286 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG | 4801 | 0.8119590181401216 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC | 4742 | 0.8019807673443985 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC | 4453 | 0.753104250734839 | RNA PCR Primer, Index 17 (95% over 21bp) |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAGATGT | 1709 | 0.2890310272862879 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1507 | 0.25486820252804904 | No Hit |
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC | 1402 | 0.23711029856955854 | RNA PCR Primer, Index 47 (95% over 23bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 1143 | 0.19330746880528205 | No Hit |
GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT | 958 | 0.16201973325937025 | TruSeq Adapter, Index 9 (95% over 21bp) |
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTA | 954 | 0.1613432416799992 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT | 878 | 0.14848990167194892 | No Hit |
GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC | 683 | 0.11551093717760948 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGTT | 20 | 7.0305035E-4 | 45.000004 | 20 |
GTATTCG | 25 | 3.8884988E-5 | 45.000004 | 26 |
TTTACGG | 50 | 2.1827873E-11 | 45.000004 | 2 |
CGCATCG | 30 | 2.163706E-6 | 45.000004 | 21 |
TTGTCGT | 20 | 7.0305035E-4 | 45.000004 | 42 |
TAACGCG | 30 | 2.163706E-6 | 45.000004 | 1 |
TATGCGA | 25 | 3.8884988E-5 | 45.000004 | 20 |
TAGGCCG | 40 | 6.8048394E-9 | 45.000004 | 1 |
AATAGCG | 25 | 3.8884988E-5 | 45.000004 | 1 |
CGCGTAA | 20 | 7.0305035E-4 | 45.000004 | 31 |
TACGGCT | 520 | 0.0 | 42.403843 | 7 |
GTCGAAT | 75 | 0.0 | 42.0 | 43 |
ACCCGTG | 65 | 0.0 | 41.53846 | 39 |
CGTATGG | 60 | 3.6379788E-12 | 41.250004 | 2 |
GCTACGA | 170 | 0.0 | 41.02941 | 10 |
GCGTTAG | 55 | 6.002665E-11 | 40.909092 | 1 |
ACGTGCG | 55 | 6.002665E-11 | 40.909092 | 1 |
GTCGTTT | 55 | 6.002665E-11 | 40.909092 | 9 |
ACGGCTG | 550 | 0.0 | 40.090908 | 8 |
CGAATAT | 175 | 0.0 | 39.857143 | 14 |