##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551838_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591286
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.244040954800216	28.0	25.0	31.0	16.0	33.0
2	27.687056686611893	31.0	25.0	33.0	16.0	34.0
3	27.499702343705078	31.0	25.0	33.0	16.0	34.0
4	31.709622754470765	35.0	28.0	37.0	19.0	37.0
5	33.09435028057488	35.0	32.0	35.0	28.0	37.0
6	32.78206823770561	35.0	32.0	35.0	28.0	37.0
7	34.149749867238526	35.0	33.0	37.0	32.0	37.0
8	34.18137077488728	35.0	35.0	37.0	31.0	37.0
9	36.504158731984184	39.0	37.0	39.0	32.0	39.0
10	35.331582347628725	37.0	34.0	39.0	31.0	39.0
11	35.83695030831104	37.0	35.0	39.0	31.0	39.0
12	35.80261328697111	37.0	35.0	39.0	31.0	39.0
13	35.965727245360114	37.0	35.0	39.0	31.0	39.0
14	36.891908484219144	39.0	36.0	40.0	31.0	41.0
15	37.05423263868923	39.0	36.0	40.0	32.0	41.0
16	36.476579861522175	38.0	34.0	40.0	31.0	41.0
17	36.70417530602788	38.0	35.0	40.0	32.0	41.0
18	36.48185480461232	38.0	35.0	40.0	31.0	41.0
19	35.997693163714345	38.0	34.0	40.0	30.0	41.0
20	36.528121754954455	38.0	35.0	40.0	31.0	41.0
21	36.71874862587648	38.0	35.0	40.0	31.0	41.0
22	37.1248211525387	39.0	35.0	40.0	32.0	41.0
23	37.46989612471799	39.0	35.0	40.0	33.0	41.0
24	37.52892339747601	39.0	35.0	41.0	33.0	41.0
25	36.53588956951458	38.0	35.0	40.0	31.0	41.0
26	36.84146588960334	39.0	35.0	40.0	31.0	41.0
27	37.084701819424104	39.0	35.0	40.0	32.0	41.0
28	37.1355283230112	39.0	35.0	40.0	32.0	41.0
29	37.24057393545594	39.0	35.0	41.0	32.0	41.0
30	36.79775945988912	39.0	35.0	40.0	31.0	41.0
31	37.00212756601712	39.0	35.0	40.0	32.0	41.0
32	36.915438552578614	39.0	35.0	40.0	32.0	41.0
33	37.083186478286315	39.0	35.0	41.0	32.0	41.0
34	37.074053841964805	39.0	35.0	41.0	32.0	41.0
35	37.018481749948414	39.0	35.0	41.0	32.0	41.0
36	36.886809428939635	39.0	35.0	41.0	31.0	41.0
37	36.88063982573577	39.0	35.0	41.0	32.0	41.0
38	36.77528810085136	39.0	35.0	41.0	31.0	41.0
39	36.74312599993912	39.0	35.0	41.0	32.0	41.0
40	36.5830883870073	39.0	35.0	40.0	31.0	41.0
41	36.4328226949395	39.0	35.0	40.0	31.0	41.0
42	36.55157910046915	39.0	35.0	40.0	31.0	41.0
43	36.52432325473629	39.0	35.0	40.0	31.0	41.0
44	36.58151892654316	39.0	35.0	40.0	31.0	41.0
45	36.59690910997385	39.0	35.0	40.0	32.0	41.0
46	36.416730651495214	39.0	35.0	40.0	31.0	41.0
47	36.32354731889475	38.0	35.0	40.0	31.0	41.0
48	36.3128976502065	38.0	35.0	40.0	31.0	41.0
49	36.27431057051918	38.0	35.0	40.0	31.0	41.0
50	36.15416059233603	38.0	35.0	40.0	31.0	41.0
51	35.214955199345155	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	21.0
10	25.0
11	20.0
12	29.0
13	35.0
14	48.0
15	78.0
16	131.0
17	206.0
18	404.0
19	795.0
20	1210.0
21	1525.0
22	1965.0
23	2751.0
24	3570.0
25	4341.0
26	5186.0
27	5901.0
28	6899.0
29	8581.0
30	11106.0
31	15566.0
32	22771.0
33	36439.0
34	49149.0
35	51380.0
36	73558.0
37	113981.0
38	133325.0
39	40283.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.70490760816254	20.40958182673021	23.607357522417242	12.278153042690002
2	32.55564988854801	25.544998528630813	24.097983040356105	17.801368542465067
3	32.34475363867908	24.260002773615476	25.829124991966662	17.566118595738782
4	27.958382238037093	25.68723088319358	27.691506309975207	18.662880568794122
5	28.808563030411676	28.37645403408841	22.867275734585295	19.947707200914618
6	26.26850627276817	36.223756354792776	22.164570106513597	15.343167265925459
7	78.57094536315759	5.949405194778838	11.106638750114158	4.373010691949411
8	79.54204902534475	8.397120851838197	7.584992710803232	4.475837412013814
9	73.21008784243158	8.066823838210274	9.10219420043769	9.620894118920456
10	44.93443105366946	23.65944737402881	16.792550474727967	14.613571097573763
11	33.28270921347706	24.134513585642143	23.524994672628814	19.05778252825198
12	29.086262823743503	22.090494278572468	27.62893083888338	21.194312058800648
13	25.943113823090684	24.865800982942265	29.085755455058976	20.105329738908075
14	21.53069749664291	28.152873567106273	27.663939278115834	22.652489658134982
15	21.21646715802505	24.40020565344013	32.620762203062476	21.762564985472345
16	23.120960076849443	23.10861410552592	29.206001833292177	24.564423984332457
17	23.498611501033338	25.80798463011132	27.72465439736439	22.968749471490955
18	23.686507037203654	24.0267823016273	28.1383289981498	24.14838166301925
19	24.985370869596103	25.95545979441421	25.04456388279107	24.01460545319862
20	26.217769404315337	24.934464878248427	28.589379758695454	20.258385958740778
21	25.590830833133204	27.97072820936061	25.653913672909557	20.784527284596624
22	24.258818913351575	24.218905910168683	27.556207994100994	23.966067182378747
23	24.786989713945538	26.223519582739996	26.6348264629976	22.35466424031687
24	23.492184831029316	24.77701146314981	28.46338320203759	23.267420503783278
25	22.214630483387058	28.385755793304764	26.50882990633974	22.890783816968437
26	22.461719032752338	26.588655912705526	27.42767459402049	23.521950460521644
27	23.71508880643208	27.40907107558779	26.841494640495462	22.034345477484667
28	21.938960164793347	27.638739966784264	28.798077410931427	21.62422245749096
29	24.109652520100255	24.891338540063522	27.56567887621219	23.433330063624034
30	22.57536961808668	28.460677235720112	27.044103868517094	21.919849277676114
31	26.251086614599366	26.478387785268044	24.482568503228556	22.787957096904034
32	26.067588273694962	28.351931214336208	24.633595248323147	20.946885263645683
33	25.67319368292163	26.570221517167663	23.992957722658748	23.763627077251957
34	24.013759838724408	27.43663810744716	25.428811099873833	23.1207909539546
35	24.26828979546277	25.567491873644904	27.503103405120367	22.66111492577196
36	27.078774061959866	27.478580585368164	24.303467357590065	21.139177995081905
37	22.75210304319737	28.959589775506267	27.781479690031556	20.506827491264804
38	24.678074569666794	27.201726406510556	25.938378382035086	22.18182064178756
39	23.688367389046924	29.653331890151296	25.89186958595333	20.76643113484845
40	23.99583281187107	26.39382633784666	28.047171757829542	21.56316909245272
41	22.729271452393597	26.582398365596344	27.175681480704768	23.51264870130529
42	21.995954580355363	25.132338665214466	28.829196023582497	24.042510730847678
43	24.945796112202892	24.56831381091384	26.960049789780243	23.525840287103026
44	23.22327942822932	25.46686375121346	27.2193151875742	24.090541632983022
45	22.892475045916864	25.72088633926729	26.673893851706282	24.712744763109562
46	24.590130664348557	27.811245319523888	25.569690471277855	22.0289335448497
47	22.66466650656366	26.112405840828295	29.7478715883684	21.475056064239638
48	23.18066045872894	25.913686439388044	28.257222393224264	22.648430708658754
49	22.6660194897224	25.145699373907043	29.467296705824253	22.720984430546302
50	22.802332542965672	25.254783641080632	28.783363719080107	23.159520096873596
51	21.992064753773978	25.89812713306251	25.988946127593078	26.120861985570436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	541.5
2	923.0
3	1978.5
4	3034.0
5	2216.0
6	1398.0
7	1414.0
8	1430.0
9	1452.5
10	1475.0
11	1461.5
12	1448.0
13	1372.0
14	1296.0
15	1272.5
16	1249.0
17	1310.0
18	1371.0
19	1314.0
20	1257.0
21	1352.5
22	1448.0
23	1655.0
24	1862.0
25	2407.0
26	3477.5
27	4003.0
28	4915.5
29	5828.0
30	7431.5
31	9035.0
32	10309.0
33	11583.0
34	13493.0
35	15403.0
36	16939.0
37	18475.0
38	19003.5
39	19532.0
40	21778.0
41	24024.0
42	26817.5
43	29611.0
44	33616.5
45	37622.0
46	48074.5
47	58527.0
48	53188.0
49	47849.0
50	46266.0
51	44683.0
52	41898.5
53	39114.0
54	37636.5
55	36159.0
56	35039.0
57	33919.0
58	31323.0
59	28727.0
60	27956.5
61	27186.0
62	25156.0
63	23126.0
64	20334.5
65	17543.0
66	14814.5
67	12086.0
68	10410.0
69	8734.0
70	7309.0
71	5884.0
72	5222.5
73	4561.0
74	3681.5
75	2305.5
76	1809.0
77	1463.0
78	1117.0
79	842.5
80	568.0
81	413.0
82	258.0
83	213.5
84	169.0
85	98.0
86	27.0
87	20.5
88	14.0
89	8.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.219126819691766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70875285848902	18.840895128792447
2	8.667245940040411	4.371607490786754
3	2.995606287829933	2.266397246239475
4	1.4823524688802647	1.4953453961669834
5	0.9498051981381758	1.197662887292456
6	0.6991866984223045	1.0579726810892207
7	0.5647471031619161	0.9969700170985739
8	0.4777048177477684	0.9637838704926972
9	0.4343073820563578	0.9857567652126854
>10	8.357596095457367	52.67822487640335
>50	0.6128076206987303	9.314677864304615
>100	0.04179765949760317	1.7916307761702968
>500	0.0033707789917421906	0.6800648229911925
>1k	0.004719090588439067	3.3590101769592478
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	4801	0.8119590181401216	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	4742	0.8019807673443985	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	4453	0.753104250734839	RNA PCR Primer, Index 17 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGAGATGT	1709	0.2890310272862879	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1507	0.25486820252804904	No Hit
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	1402	0.23711029856955854	RNA PCR Primer, Index 47 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1143	0.19330746880528205	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCT	958	0.16201973325937025	TruSeq Adapter, Index 9 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTA	954	0.1613432416799992	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCT	878	0.14848990167194892	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTC	683	0.11551093717760948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06663442056804998	0.0
2	0.0	0.0	0.0	0.4562935702857839	0.0
3	0.0	0.0	0.0	0.570620647199494	0.0
4	0.0	0.0	0.0	0.892123270295593	0.0
5	0.0	0.0	0.0	2.0374235141708072	0.0
6	0.0	0.0	0.0	2.3278075246158374	0.0
7	0.0	0.0	0.0	2.735224578292062	0.0
8	0.0	0.0	0.0	3.261535027042751	0.0
9	0.0	0.0	0.0	3.4364081003101714	0.0
10	0.0	0.0	0.0	4.666608037396455	0.0
11	0.0	0.0	0.0	5.265133962245005	0.0
12	0.0	0.0	0.0	6.554865158315942	0.0
13	0.0	0.0	0.0	6.797894758204997	0.0
14	0.0	0.0	0.0	6.9113762206444935	0.0
15	0.0	0.0	0.0	7.227297788210781	0.0
16	0.0	0.0	0.0	7.5733232310590815	0.0
17	0.0	0.0	0.0	7.992917133163985	0.0
18	0.0	0.0	0.0	8.397120851838197	0.0
19	0.0	0.0	0.0	8.956917633767754	0.0
20	0.0	0.0	0.0	9.253559191321965	0.0
21	0.0	0.0	0.0	9.556627418880204	0.0
22	0.0	0.0	0.0	9.902821984623346	0.0
23	0.0	0.0	0.0	10.243773740626363	0.0
24	0.0	0.0	0.0	10.528914941331267	0.0
25	0.0	0.0	0.0	10.779385948593404	0.0
26	0.0	0.0	0.0	11.006348873472398	0.0
27	0.0	0.0	0.0	11.271026203901327	0.0
28	0.0	0.0	0.0	11.497820005885476	0.0
29	0.0	0.0	0.0	11.72664328260774	0.0
30	0.0	0.0	0.0	11.990982367246984	0.0
31	0.0	0.0	0.0	12.273924970318932	0.0
32	1.6912289484276644E-4	0.0	0.0	12.544352479172515	0.0
33	1.6912289484276644E-4	0.0	0.0	12.804294368545847	0.0
34	1.6912289484276644E-4	0.0	0.0	13.06609660976245	0.0
35	1.6912289484276644E-4	0.0	0.0	13.336524118616033	0.0
36	1.6912289484276644E-4	0.0	0.0	13.590377583775027	0.0
37	1.6912289484276644E-4	0.0	0.0	13.88346756053754	0.0
38	1.6912289484276644E-4	0.0	0.0	14.154571560970496	0.0
39	1.6912289484276644E-4	0.0	0.0	14.427874159036405	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	20	7.0305035E-4	45.000004	20
GTATTCG	25	3.8884988E-5	45.000004	26
TTTACGG	50	2.1827873E-11	45.000004	2
CGCATCG	30	2.163706E-6	45.000004	21
TTGTCGT	20	7.0305035E-4	45.000004	42
TAACGCG	30	2.163706E-6	45.000004	1
TATGCGA	25	3.8884988E-5	45.000004	20
TAGGCCG	40	6.8048394E-9	45.000004	1
AATAGCG	25	3.8884988E-5	45.000004	1
CGCGTAA	20	7.0305035E-4	45.000004	31
TACGGCT	520	0.0	42.403843	7
GTCGAAT	75	0.0	42.0	43
ACCCGTG	65	0.0	41.53846	39
CGTATGG	60	3.6379788E-12	41.250004	2
GCTACGA	170	0.0	41.02941	10
GCGTTAG	55	6.002665E-11	40.909092	1
ACGTGCG	55	6.002665E-11	40.909092	1
GTCGTTT	55	6.002665E-11	40.909092	9
ACGGCTG	550	0.0	40.090908	8
CGAATAT	175	0.0	39.857143	14
>>END_MODULE