FastQCFastQC Report
Sat 18 Jun 2016
SRR3551837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274309
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG21060.7677473214513559No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC19780.7210846162539326TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC17530.6390603297740869No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.4451184612972961No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC9930.3620005176643858TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC6840.249353830898731TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT4850.1768079064121119No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT4720.17206872541549859No Hit
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT4340.15821573481001353TruSeq Adapter, Index 20 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT3880.14144632512968952No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC3320.12103139160581679No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTA3310.12066683922146192No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3040.11082392484388044No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC3030.11045937245952556No Hit
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC2930.10681384861597687TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTTA253.8830152E-545.00000414
CGTATTC207.023885E-445.00000445
CATATGA207.023885E-445.00000410
ATAGGTC207.023885E-445.00000429
ACTTGCG207.023885E-445.0000041
CGGCCCA207.023885E-445.00000421
TTTCGCT207.023885E-445.00000414
ACTCTAT207.023885E-445.0000049
TCTTACC207.023885E-445.00000443
AAGACGT207.023885E-445.00000434
TACGGGC207.023885E-445.0000044
CCTCGTG207.023885E-445.00000432
TCGATAG253.8830152E-545.0000041
CGTTATC207.023885E-445.00000439
CATTAGC207.023885E-445.00000436
ATCCGCG207.023885E-445.00000414
CCCAATT207.023885E-445.00000435
GCCGACA207.023885E-445.00000436
TGCGACG207.023885E-445.0000041
GCCTCGT207.023885E-445.00000431