##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551837_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 274309 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.391186581555836 28.0 25.0 31.0 16.0 33.0 2 27.750941456532598 31.0 25.0 33.0 16.0 34.0 3 27.57078331370826 31.0 25.0 33.0 16.0 34.0 4 31.665377366400666 35.0 28.0 37.0 19.0 37.0 5 33.24084517824789 35.0 32.0 35.0 28.0 37.0 6 33.02530358099807 35.0 32.0 35.0 28.0 37.0 7 34.05196694238979 35.0 33.0 37.0 31.0 37.0 8 34.28578719619116 35.0 35.0 37.0 31.0 37.0 9 36.384489754255235 39.0 37.0 39.0 32.0 39.0 10 35.451687695263374 37.0 34.0 39.0 31.0 39.0 11 35.94580199701796 37.0 35.0 39.0 31.0 39.0 12 35.775570615619614 37.0 35.0 39.0 31.0 39.0 13 35.89443656606236 37.0 35.0 39.0 31.0 39.0 14 36.75551658895625 39.0 36.0 40.0 31.0 41.0 15 37.0841131716422 39.0 35.0 40.0 32.0 41.0 16 36.49633078025147 38.0 34.0 40.0 31.0 41.0 17 36.8050191572278 38.0 35.0 40.0 32.0 41.0 18 36.58042207875061 38.0 35.0 40.0 31.0 41.0 19 35.9645983179553 37.0 34.0 40.0 30.0 41.0 20 36.604544510023366 38.0 35.0 40.0 32.0 41.0 21 36.72091692215713 38.0 35.0 40.0 32.0 41.0 22 37.13109668293785 39.0 35.0 40.0 32.0 41.0 23 37.47134800535163 39.0 35.0 40.0 33.0 41.0 24 37.51527656766639 39.0 35.0 41.0 33.0 41.0 25 36.5669773868156 38.0 35.0 40.0 31.0 41.0 26 36.80711897896168 38.0 35.0 40.0 32.0 41.0 27 37.13182943323041 39.0 35.0 40.0 33.0 41.0 28 37.1807159079724 39.0 35.0 40.0 33.0 41.0 29 37.289946738896646 39.0 35.0 41.0 33.0 41.0 30 36.84682237914177 39.0 35.0 40.0 32.0 41.0 31 36.96565187434609 39.0 35.0 40.0 32.0 41.0 32 36.90646679474607 39.0 35.0 40.0 32.0 41.0 33 36.9539752614752 39.0 35.0 41.0 32.0 41.0 34 36.94599520978167 39.0 35.0 41.0 33.0 41.0 35 36.81298098130211 39.0 35.0 41.0 32.0 41.0 36 36.644328111728015 39.0 35.0 41.0 31.0 41.0 37 36.675118206110625 39.0 35.0 41.0 32.0 41.0 38 36.57653959585723 39.0 35.0 41.0 31.0 41.0 39 36.4957219777696 39.0 35.0 40.0 31.0 41.0 40 36.33972272145646 39.0 35.0 40.0 31.0 41.0 41 36.1830854984707 39.0 35.0 40.0 30.0 41.0 42 36.291313810337975 39.0 35.0 40.0 31.0 41.0 43 36.211141449970654 39.0 35.0 40.0 31.0 41.0 44 36.22073646872688 39.0 35.0 40.0 31.0 41.0 45 36.23729079250043 38.0 35.0 40.0 31.0 41.0 46 36.053669402024724 38.0 35.0 40.0 31.0 41.0 47 35.9848820126208 38.0 35.0 40.0 31.0 41.0 48 36.0206518925737 38.0 35.0 40.0 31.0 41.0 49 36.00167694096803 38.0 35.0 40.0 31.0 41.0 50 35.8664717526585 38.0 35.0 40.0 31.0 41.0 51 34.89792168685679 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 5.0 10 12.0 11 13.0 12 15.0 13 15.0 14 18.0 15 21.0 16 56.0 17 64.0 18 123.0 19 197.0 20 357.0 21 617.0 22 916.0 23 1228.0 24 1727.0 25 2324.0 26 2851.0 27 3107.0 28 3449.0 29 4074.0 30 5122.0 31 7158.0 32 10905.0 33 18250.0 34 24939.0 35 24134.0 36 33350.0 37 51499.0 38 60367.0 39 17394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.933367115187615 20.245052112763343 23.390410084977162 14.431170687071878 2 33.56433802755287 24.71738076402889 23.863599079869783 17.854682128548465 3 30.657761867091494 23.16949134005811 27.897735765140773 18.275011027709624 4 29.36031993117251 24.420635123164022 27.718375992038176 18.50066895362529 5 27.138008596145223 27.590053552745264 24.669988954062756 20.60194889704676 6 25.25983471194893 33.4808555315356 25.923320051474796 15.335989705040664 7 76.19728116831747 5.918507960001312 13.126073151081444 4.758137720599762 8 76.29498120732458 6.9213915693615595 10.810071853274957 5.973555370038898 9 69.87995289983195 7.705543748108884 12.520916193052361 9.893587159006813 10 44.356911366378796 20.070796073041716 19.855345613887987 15.716946946691504 11 33.54829772264126 22.2107185692048 25.186924235077964 19.054059473075984 12 29.372350159856218 20.331815580239805 30.042397442300473 20.253436817603507 13 24.097277158241255 25.670320696732517 31.67048839082932 18.561913754196908 14 19.94283818613316 28.390610588788554 30.072655290201926 21.59389593487636 15 18.57540219241804 23.091841682190523 37.94844500180453 20.384311123586905 16 20.852031832714204 22.450594038110307 33.828638506210154 22.868735622965342 17 21.27272528425972 24.006503614536893 28.388423274482427 26.33234782672096 18 22.84904979421018 23.04153345314955 32.1965374814534 21.91287927118687 19 24.024731233754633 24.204820111625942 28.273224721026285 23.497223933593137 20 26.350575445938706 23.323332446255865 30.43611401740373 19.8899780904017 21 23.625546372886053 26.012999938026095 29.681126029404798 20.68032765968306 22 23.291251836432636 22.360914151559008 29.651961838656405 24.695872173351948 23 22.52642093405612 25.231399625969253 29.24147585387282 23.000703586101807 24 23.332446255864735 22.42616902835853 31.561851780291565 22.679532935485163 25 20.04710016805865 26.15845633938369 29.759504792041092 24.03493870051657 26 20.88083147107824 26.473794151850647 30.269149025369202 22.376225351701912 27 22.09369725382689 26.363699331775482 29.428855779431224 22.11374763496641 28 19.23123193187245 24.552603086300486 33.08640985166363 23.129755130163428 29 22.12140323503786 22.866183756274857 31.95848477446967 23.053928234217615 30 21.174296140483907 25.914935346634632 30.425906550641795 22.484861962239663 31 23.702831478369284 24.377617941810147 27.529537856942355 24.39001272287821 32 24.495003809572417 24.727588230790822 29.002694042120382 21.77471391751638 33 23.198290978422147 24.0145237669927 27.39538257949976 25.391802675085394 34 22.310605922518036 23.817665479441068 29.968757860660787 23.90297073738011 35 21.297150294011498 22.881130404033406 32.57494285641375 23.246776445541343 36 25.742137516450427 23.425042561490876 28.422691198611783 22.410128723446913 37 20.930775147734852 24.341527255759015 32.52499917975714 22.202698416748994 38 21.271996179491012 24.540937410001128 28.144537729349018 26.04252868115884 39 23.13959804454101 23.406450389888775 30.47949575114196 22.974455814428254 40 22.305866741521424 23.683145649614122 31.104703090310565 22.906284518553893 41 22.66057621149871 23.044085319840036 29.048627642549096 25.246710826112157 42 23.961299118876887 22.238789102800126 30.1568668909879 23.643044887335087 43 24.359754874976762 23.32661341771506 29.16200343408346 23.15162827322472 44 21.62306012562475 24.09180887247593 29.36724642647525 24.917884575424065 45 21.644933268686046 23.85885989887317 28.47773860864937 26.01846822379142 46 23.848287879726875 23.98390136670689 29.52801402797575 22.639796725590482 47 21.069305053789705 22.875297565883727 32.79622615371716 23.259171226609407 48 22.970081185815996 21.37078987565118 31.8822933261395 23.776835612393324 49 21.943866223857036 20.806462784669844 32.82065116346893 24.429019828004183 50 21.264705131803915 22.962425585744544 30.39783601704647 25.375033265405072 51 21.10211476838164 23.3634332085349 27.841230145565767 27.693221877517686 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 211.0 1 325.5 2 440.0 3 1364.5 4 2289.0 5 1667.5 6 1046.0 7 1092.0 8 1138.0 9 1177.5 10 1217.0 11 1192.0 12 1167.0 13 1143.5 14 1120.0 15 1078.0 16 1036.0 17 926.5 18 817.0 19 836.5 20 856.0 21 816.5 22 777.0 23 937.5 24 1098.0 25 1220.5 26 1560.0 27 1777.0 28 1962.5 29 2148.0 30 2689.5 31 3231.0 32 3868.0 33 4505.0 34 4811.0 35 5117.0 36 6188.5 37 7260.0 38 7600.0 39 7940.0 40 9109.5 41 10279.0 42 12490.5 43 14702.0 44 17076.5 45 19451.0 46 24369.5 47 29288.0 48 27490.0 49 25692.0 50 25398.5 51 25105.0 52 21708.5 53 18312.0 54 16945.5 55 15579.0 56 14280.5 57 12982.0 58 12934.5 59 12887.0 60 11835.5 61 10784.0 62 10010.5 63 9237.0 64 8374.0 65 7511.0 66 6308.0 67 5105.0 68 4541.0 69 3977.0 70 3282.0 71 2587.0 72 2153.5 73 1720.0 74 1416.0 75 914.0 76 716.0 77 603.0 78 490.0 79 320.5 80 151.0 81 120.5 82 90.0 83 50.5 84 11.0 85 8.0 86 5.0 87 3.0 88 1.0 89 1.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 274309.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.06682245205225 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80600417091033 22.491788457542405 2 10.622484116591494 6.387686878666029 3 3.7477569232261505 3.380494260122709 4 1.750812357534313 2.1056545720337283 5 0.9711916193801833 1.4600322993412538 6 0.6001745962461807 1.0827205815339636 7 0.4243658761336631 0.8931533416694312 8 0.36252970561132936 0.8720093033768488 9 0.29948106115718515 0.8103999504208756 >10 5.7798632329404915 44.36821249029379 >50 0.5965371744507493 10.901939054132384 >100 0.03152432222707212 2.061543733526789 >500 0.0024249478636209324 0.6113543485631168 >1k 0.004849895727241865 2.5730107287766715 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG 2106 0.7677473214513559 No Hit GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC 1978 0.7210846162539326 TruSeq Adapter, Index 23 (95% over 21bp) GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC 1753 0.6390603297740869 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1221 0.4451184612972961 No Hit GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC 993 0.3620005176643858 TruSeq Adapter, Index 23 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC 684 0.249353830898731 TruSeq Adapter, Index 23 (95% over 24bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT 485 0.1768079064121119 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT 472 0.17206872541549859 No Hit CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT 434 0.15821573481001353 TruSeq Adapter, Index 20 (95% over 23bp) GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT 388 0.14144632512968952 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC 332 0.12103139160581679 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTA 331 0.12066683922146192 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 304 0.11082392484388044 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC 303 0.11045937245952556 No Hit TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC 293 0.10681384861597687 TruSeq Adapter, Index 23 (95% over 24bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.16295491580662683 0.0 2 0.0 0.0 0.0 0.9704384471526636 0.0 3 0.0 0.0 0.0 1.2978064883033367 0.0 4 0.0 0.0 0.0 1.8978597129514525 0.0 5 0.0 0.0 0.0 3.6772399009875723 0.0 6 0.0 0.0 0.0 4.353120021581502 0.0 7 0.0 0.0 0.0 5.151125190934311 0.0 8 0.0 0.0 0.0 6.192651353036174 0.0 9 0.0 0.0 0.0 6.581628747142821 0.0 10 0.0 0.0 0.0 8.231957391117316 0.0 11 0.0 0.0 0.0 9.577884794155496 0.0 12 0.0 0.0 0.0 11.227484333361282 0.0 13 0.0 0.0 0.0 11.702496090175678 0.0 14 0.0 0.0 0.0 11.873106606053756 0.0 15 0.0 0.0 0.0 12.316766857813633 0.0 16 0.0 0.0 0.0 13.041496997911114 0.0 17 0.0 0.0 0.0 13.839502167263925 0.0 18 0.0 0.0 0.0 14.766558880678359 0.0 19 0.0 0.0 0.0 15.456656544262128 0.0 20 0.0 0.0 0.0 15.957916072750074 0.0 21 0.0 0.0 0.0 16.58494617384045 0.0 22 0.0 0.0 0.0 17.215621798774375 0.0 23 0.0 0.0 0.0 17.799634718510877 0.0 24 0.0 0.0 0.0 18.30453977084237 0.0 25 0.0 0.0 0.0 18.730701508153214 0.0 26 0.0 0.0 0.0 19.137177416708894 0.0 27 0.0 0.0 0.0 19.54656974433941 0.0 28 0.0 0.0 0.0 19.93773445275219 0.0 29 0.0 0.0 0.0 20.3318155802398 0.0 30 0.0 0.0 0.0 20.78969337498952 0.0 31 0.0 0.0 0.0 21.26069505557601 0.0 32 0.0 0.0 0.0 21.693054183420887 0.0 33 0.0 0.0 0.0 22.09078083475205 0.0 34 0.0 0.0 0.0 22.48085188601176 0.0 35 0.0 0.0 0.0 22.958050957132286 0.0 36 0.0 0.0 0.0 23.373276122912483 0.0 37 0.0 0.0 0.0 23.778658374315096 0.0 38 0.0 0.0 0.0 24.15524098735368 0.0 39 0.0 0.0 0.0 24.546041143382098 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACTTA 25 3.8830152E-5 45.000004 14 CGTATTC 20 7.023885E-4 45.000004 45 CATATGA 20 7.023885E-4 45.000004 10 ATAGGTC 20 7.023885E-4 45.000004 29 ACTTGCG 20 7.023885E-4 45.000004 1 CGGCCCA 20 7.023885E-4 45.000004 21 TTTCGCT 20 7.023885E-4 45.000004 14 ACTCTAT 20 7.023885E-4 45.000004 9 TCTTACC 20 7.023885E-4 45.000004 43 AAGACGT 20 7.023885E-4 45.000004 34 TACGGGC 20 7.023885E-4 45.000004 4 CCTCGTG 20 7.023885E-4 45.000004 32 TCGATAG 25 3.8830152E-5 45.000004 1 CGTTATC 20 7.023885E-4 45.000004 39 CATTAGC 20 7.023885E-4 45.000004 36 ATCCGCG 20 7.023885E-4 45.000004 14 CCCAATT 20 7.023885E-4 45.000004 35 GCCGACA 20 7.023885E-4 45.000004 36 TGCGACG 20 7.023885E-4 45.000004 1 GCCTCGT 20 7.023885E-4 45.000004 31 >>END_MODULE