FastQCFastQC Report
Sat 18 Jun 2016
SRR3551835_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551835_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430963
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13350.30977137248441283No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG11350.2633636762320663No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC10480.24317632836229558No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC9870.22902198100532992No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC7300.1693880913210647TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4970.11532312518708103No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC4730.10975420163679944TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAAC207.0283737E-445.09
TCGTTAT207.0283737E-445.019
CTCGTTA207.0283737E-445.018
ATTAGCG453.8380676E-1045.01
TTGTCGA207.0283737E-445.033
ATAGCCG253.8867332E-545.01
CGTTATC207.0283737E-445.020
AACGGGT253.8867332E-545.04
TAACCCG207.0283737E-445.017
TTAAGCG207.0283737E-445.01
TCTCGTT207.0283737E-445.017
TCGTCGG207.0283737E-445.02
GTCCGTC207.0283737E-445.06
GCGTGAT207.0283737E-445.043
TAGGACG207.0283737E-445.01
GACGACT207.0283737E-445.010
ATCATGC207.0283737E-445.031
CGATACG253.8867332E-545.010
TGCACCG253.8867332E-545.01
GTCGGTT207.0283737E-445.028