##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551835_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430963
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.268440678202072	28.0	25.0	31.0	16.0	33.0
2	27.72185547251156	31.0	25.0	33.0	16.0	34.0
3	27.49027874782754	31.0	25.0	33.0	16.0	34.0
4	31.712035603984564	35.0	28.0	37.0	19.0	37.0
5	33.20990665091899	35.0	32.0	35.0	28.0	37.0
6	32.839306390571814	35.0	32.0	35.0	28.0	37.0
7	34.15437288119862	35.0	33.0	37.0	31.0	37.0
8	34.2412457681982	35.0	35.0	37.0	31.0	37.0
9	36.59112035139908	39.0	37.0	39.0	32.0	39.0
10	35.557959732041965	37.0	34.0	39.0	31.0	39.0
11	36.00385879994338	37.0	35.0	39.0	31.0	39.0
12	35.93356274204514	37.0	35.0	39.0	31.0	39.0
13	36.02951065404687	37.0	35.0	39.0	32.0	39.0
14	36.84166390154143	39.0	36.0	40.0	31.0	41.0
15	37.1588929908136	39.0	36.0	40.0	32.0	41.0
16	36.72819244343482	38.0	35.0	40.0	32.0	41.0
17	36.990268306095885	38.0	35.0	40.0	32.0	41.0
18	36.683576084257815	38.0	35.0	40.0	32.0	41.0
19	36.23446560377573	38.0	34.0	40.0	30.0	41.0
20	36.85435640646645	38.0	35.0	40.0	32.0	41.0
21	36.84856936674378	38.0	35.0	40.0	32.0	41.0
22	37.288458637980526	39.0	35.0	40.0	33.0	41.0
23	37.65872476291468	39.0	35.0	40.0	33.0	41.0
24	37.74464165137147	39.0	35.0	41.0	34.0	41.0
25	36.67202288827579	38.0	35.0	40.0	31.0	41.0
26	36.97930448785627	39.0	35.0	40.0	32.0	41.0
27	37.306172455640045	39.0	35.0	40.0	33.0	41.0
28	37.356230117202635	39.0	35.0	40.0	33.0	41.0
29	37.46281235280059	39.0	35.0	41.0	33.0	41.0
30	37.044117476442295	39.0	35.0	40.0	32.0	41.0
31	37.17491292755991	39.0	35.0	40.0	33.0	41.0
32	37.168295190074325	39.0	35.0	40.0	33.0	41.0
33	37.3113329914633	39.0	35.0	41.0	33.0	41.0
34	37.337479087531875	40.0	35.0	41.0	33.0	41.0
35	37.278750147924534	40.0	35.0	41.0	33.0	41.0
36	37.168190772757754	40.0	35.0	41.0	33.0	41.0
37	37.23010328032801	40.0	35.0	41.0	33.0	41.0
38	37.08146407000137	39.0	35.0	41.0	33.0	41.0
39	37.02218519919344	39.0	35.0	41.0	33.0	41.0
40	36.8570874993909	39.0	35.0	41.0	32.0	41.0
41	36.73155236064349	39.0	35.0	40.0	32.0	41.0
42	36.83333604044895	39.0	35.0	41.0	32.0	41.0
43	36.80689061473955	39.0	35.0	41.0	32.0	41.0
44	36.79421435250822	39.0	35.0	41.0	32.0	41.0
45	36.79349503321631	39.0	35.0	41.0	33.0	41.0
46	36.626547986718116	39.0	35.0	40.0	32.0	41.0
47	36.6115931066008	39.0	35.0	40.0	32.0	41.0
48	36.58238178219476	39.0	35.0	40.0	32.0	41.0
49	36.53147718017556	38.0	35.0	40.0	32.0	41.0
50	36.42821077447484	38.0	35.0	40.0	32.0	41.0
51	35.42018688379281	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	17.0
11	20.0
12	19.0
13	28.0
14	41.0
15	49.0
16	64.0
17	113.0
18	169.0
19	311.0
20	474.0
21	769.0
22	1013.0
23	1391.0
24	2012.0
25	2730.0
26	3515.0
27	4090.0
28	4697.0
29	5622.0
30	7469.0
31	10480.0
32	15959.0
33	27407.0
34	38109.0
35	35607.0
36	51185.0
37	82761.0
38	102652.0
39	32171.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23518028229802	19.608179820541437	24.911419309778335	13.245220587382212
2	33.38128795279409	23.176931662346885	25.268294493958877	18.173485890900146
3	34.20572067671703	21.874267628543517	26.04283894441054	17.877172750328914
4	30.291927613275387	25.329320614530715	24.369145379069664	20.009606393124237
5	26.60251576121384	29.34683487909635	24.36752110970083	19.68312824998898
6	27.39655144409149	34.377197114369444	23.24468689887531	14.981564542663756
7	79.4098333267589	6.61773748558461	9.341173140153563	4.631256047502918
8	80.36815225436986	6.363887387084274	8.469172527571972	4.798787830973889
9	73.79194965693111	8.821406942127282	10.272575603938156	7.114067797003456
10	43.712569292491466	24.40603949759028	17.352301705714876	14.529089504203377
11	34.87422354123208	25.377816657114415	22.214204003591956	17.533755798061552
12	33.09704081324847	22.55089183990273	25.847694581669423	18.504372765179376
13	26.671431190148574	25.6404842179027	27.203727466162984	20.484357125785742
14	20.962820474147435	29.11479639783462	27.175882848411582	22.74650027960637
15	20.554200708645524	25.91544981819785	33.38360833760671	20.14674113554992
16	23.58647958177384	24.387940496051865	32.258453741968566	19.767126180205725
17	23.565596118460284	24.25475040780763	28.355334448664966	23.824319025067116
18	23.199903471991796	26.359803509814068	28.391300413260534	22.048992604933602
19	26.68024865243652	26.69741950004989	25.445107816680316	21.177224030833273
20	28.635172857066614	26.29483273506078	26.786754315335653	18.283240092536946
21	26.87956970784035	27.192821657543686	25.501725206108183	20.425883428507785
22	24.775444759758958	25.544652325141605	26.889547362534604	22.790355552564836
23	24.48493258121927	27.05383060726791	26.924353134723862	21.53688367678896
24	24.39536572745224	25.721233609381777	27.748089743203014	22.135310919962965
25	23.641936778795394	26.307826890011437	27.263361355847255	22.78687497534591
26	22.299826203177535	29.596972361896494	26.674911767367497	21.428289667558467
27	23.581606773667346	29.51599093193615	26.57327891257486	20.32912338182164
28	21.39510816473804	29.173734172075093	28.465088650301766	20.966069012885097
29	23.984889654100236	27.81839740302532	27.386805827878497	20.80990711499595
30	24.156598130233917	26.39066462782188	27.21068862060084	22.242048621343365
31	24.572411088654942	27.8724623691593	24.007165348301363	23.547961193884394
32	26.861006629339414	28.578787506120012	24.287467833665534	20.27273803087504
33	26.117555335376817	27.86410898383388	24.61116151502565	21.40717416576365
34	23.490879727494008	27.858540060283598	25.992486593976743	22.65809361824565
35	23.91991887934695	26.892795901272265	25.967194399519215	23.220090819861568
36	23.124955042544258	30.466652589665472	25.024422050152793	21.383970317637477
37	24.777533106090313	28.93334230548794	25.561127057311182	20.72799753111056
38	23.62360573877572	27.812828479475037	25.834236349756246	22.729329431993
39	24.573803319542513	26.20201734255609	26.836178511844405	22.388000826056995
40	23.189925817297542	26.12033979715196	29.198330251088837	21.491404134461657
41	20.574388056515293	27.647617080816683	28.155781354779876	23.622213507888148
42	22.33300770599796	27.578237574919424	26.755893197327847	23.33286152175477
43	24.46219281005562	26.69602726916232	26.25422600083998	22.58755391994208
44	22.623055807575128	27.26684193306618	26.709021424112976	23.401080835245715
45	23.033531880927132	26.855205667307867	25.680162798198452	24.431099653566548
46	21.926476286827405	29.272814603573856	26.25074542362105	22.549963685977684
47	22.429767752684103	26.786290238373134	28.10078823472085	22.683153774221918
48	23.056735729053308	24.70745748474927	28.652343704679982	23.58346308151744
49	21.891438476156885	26.564229411805655	28.24140355436546	23.302928557672004
50	20.987416553161175	26.500186790977416	28.040226191111532	24.472170464749873
51	21.308557811227413	26.41758109164824	26.375350088058603	25.898511009065743
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	594.5
2	1029.0
3	1900.0
4	2771.0
5	1990.0
6	1209.0
7	1276.0
8	1343.0
9	1316.5
10	1290.0
11	1288.0
12	1286.0
13	1199.0
14	1112.0
15	1099.0
16	1086.0
17	1097.5
18	1109.0
19	1044.0
20	979.0
21	1359.0
22	1739.0
23	1740.5
24	1742.0
25	2307.5
26	3096.5
27	3320.0
28	4077.0
29	4834.0
30	5838.5
31	6843.0
32	8048.0
33	9253.0
34	9888.0
35	10523.0
36	11108.0
37	11693.0
38	13058.0
39	14423.0
40	15735.5
41	17048.0
42	19691.0
43	22334.0
44	25958.0
45	29582.0
46	32447.0
47	35312.0
48	34119.0
49	32926.0
50	32322.5
51	31719.0
52	30470.5
53	29222.0
54	27645.5
55	26069.0
56	24631.0
57	23193.0
58	23234.0
59	23275.0
60	24137.0
61	24999.0
62	22364.0
63	19729.0
64	16497.5
65	13266.0
66	10665.5
67	8065.0
68	6722.0
69	5379.0
70	4178.5
71	2978.0
72	2636.5
73	2295.0
74	1692.0
75	961.5
76	834.0
77	707.5
78	581.0
79	448.0
80	315.0
81	204.5
82	94.0
83	54.5
84	15.0
85	17.5
86	20.0
87	13.0
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.687691942075396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26239813014293	17.117294259024305
2	10.660215331437785	5.050317936145747
3	3.5035056995964644	2.489698911880392
4	1.714459893969289	1.6244639126155103
5	0.989041017404187	1.1714049469173604
6	0.6903260189058728	0.9811338045264979
7	0.46954127923725647	0.7785644423662094
8	0.38961933619482914	0.7383346248367215
9	0.35565252027850464	0.7582128604901945
>10	7.7284491440449745	48.01257514036909
>50	1.1698570252913243	17.139775601628752
>100	0.06193948383267473	2.899383258838508
>500	0.0019980478655701526	0.40632068686138734
>1k	0.0029970717983552287	0.8325196134993366
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1335	0.30977137248441283	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	1135	0.2633636762320663	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	1048	0.24317632836229558	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	987	0.22902198100532992	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	730	0.1693880913210647	TruSeq Adapter, Index 16 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	497	0.11532312518708103	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	473	0.10975420163679944	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09467170035478684	0.0
2	0.0	0.0	0.0	0.4803196562117862	0.0
3	0.0	0.0	0.0	0.6167582831936849	0.0
4	0.0	0.0	0.0	0.8945083452639786	0.0
5	0.0	0.0	0.0	1.6943449901731704	0.0
6	0.0	0.0	0.0	2.0254639029336623	0.0
7	0.0	0.0	0.0	2.420393398041131	0.0
8	0.0	0.0	0.0	2.985871176875973	0.0
9	0.0	0.0	0.0	3.189600963423774	0.0
10	0.0	0.0	0.0	3.9516153358873036	0.0
11	0.0	0.0	0.0	4.741010249139717	0.0
12	0.0	0.0	0.0	5.584934205488638	0.0
13	0.0	0.0	0.0	5.8524745743834155	0.0
14	0.0	0.0	0.0	5.955963737026148	0.0
15	0.0	0.0	0.0	6.188698333731666	0.0
16	0.0	0.0	0.0	6.593837522014651	0.0
17	0.0	0.0	0.0	7.046080521993767	0.0
18	0.0	0.0	0.0	7.564222450651216	0.0
19	0.0	0.0	0.0	7.90763940291858	0.0
20	0.0	0.0	0.0	8.2343495845351	0.0
21	0.0	0.0	0.0	8.61094803962289	0.0
22	0.0	0.0	0.0	9.00448530384279	0.0
23	0.0	0.0	0.0	9.396398298693855	0.0
24	0.0	0.0	0.0	9.712434710172335	0.0
25	2.3203848126173245E-4	0.0	0.0	10.007355619855996	0.0
26	2.3203848126173245E-4	0.0	0.0	10.284873643445028	0.0
27	2.3203848126173245E-4	0.0	0.0	10.534779087763916	0.0
28	2.3203848126173245E-4	0.0	0.0	10.809744688059068	0.0
29	2.3203848126173245E-4	0.0	0.0	11.070787979478517	0.0
30	2.3203848126173245E-4	0.0	0.0	11.371973928156246	0.0
31	2.3203848126173245E-4	0.0	0.0	11.698916148254026	0.0
32	2.3203848126173245E-4	0.0	0.0	12.019129252395217	0.0
33	2.3203848126173245E-4	0.0	0.0	12.293862814209108	0.0
34	2.3203848126173245E-4	0.0	0.0	12.594816724405575	0.0
35	2.3203848126173245E-4	0.0	0.0	12.913405559177933	0.0
36	2.3203848126173245E-4	0.0	0.0	13.249397280044922	0.0
37	2.3203848126173245E-4	0.0	0.0	13.588637539649575	0.0
38	2.3203848126173245E-4	0.0	0.0	13.910010836197076	0.0
39	2.3203848126173245E-4	0.0	0.0	14.233936556038453	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	20	7.0283737E-4	45.0	9
TCGTTAT	20	7.0283737E-4	45.0	19
CTCGTTA	20	7.0283737E-4	45.0	18
ATTAGCG	45	3.8380676E-10	45.0	1
TTGTCGA	20	7.0283737E-4	45.0	33
ATAGCCG	25	3.8867332E-5	45.0	1
CGTTATC	20	7.0283737E-4	45.0	20
AACGGGT	25	3.8867332E-5	45.0	4
TAACCCG	20	7.0283737E-4	45.0	17
TTAAGCG	20	7.0283737E-4	45.0	1
TCTCGTT	20	7.0283737E-4	45.0	17
TCGTCGG	20	7.0283737E-4	45.0	2
GTCCGTC	20	7.0283737E-4	45.0	6
GCGTGAT	20	7.0283737E-4	45.0	43
TAGGACG	20	7.0283737E-4	45.0	1
GACGACT	20	7.0283737E-4	45.0	10
ATCATGC	20	7.0283737E-4	45.0	31
CGATACG	25	3.8867332E-5	45.0	10
TGCACCG	25	3.8867332E-5	45.0	1
GTCGGTT	20	7.0283737E-4	45.0	28
>>END_MODULE