FastQCFastQC Report
Sat 18 Jun 2016
SRR3551830_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551830_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences756000
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCG54870.7257936507936508No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTC54120.7158730158730159No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGC47880.6333333333333333No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24480.3238095238095238No Hit
GCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC17680.23386243386243388No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTCAAAGT15930.21071428571428574No Hit
GAACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCT13610.18002645502645503No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCT10600.1402116402116402No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTA10060.13306878306878306No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTC8670.11468253968253968No Hit
CCTGTCTCTTATACACATCTGACGCTGTCAAAGTCGTATGCCGTCTTCTGC8170.10806878306878306No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG207.031752E-445.0000041
TGCGAAC207.031752E-445.00000441
TCGAAAG207.031752E-445.0000041
CGAATAT700.045.014
GCGATAT351.2111923E-745.09
GCTACGA700.045.010
TATAGCG302.164512E-644.9999961
TACGGCT5850.041.9230777
TGTAGCG501.0804797E-940.51
CGTATGG451.9268555E-840.02
AGACCGG451.9268555E-840.02
TCGTAAG451.9268555E-840.01
CGTTAGG850.039.7058832
CGTTTTT18050.039.639891
ACGGCTG6250.039.248
CTATCGG356.245453E-638.5714262
CTAGGCG356.245453E-638.5714261
TAGTACG356.245453E-638.5714261
GGGCGAT9350.038.5026747
ATAGCGG1000.038.252