SRR3551827_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925787
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	4395	0.47473122867355017	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	4278	0.4620933324836058	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	4114	0.44437867457633345	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2162	0.23353103899709113	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1732	0.18708407009387687	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1525	0.16472471529628305	TruSeq Adapter, Index 18 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1188	0.1283232536209733	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1050	0.11341701708924407	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGCAACT	955	0.10315547744783628	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.890216E-5	45.0	1
TATGCGC	20	7.032576E-4	45.0	18
GTATCAC	20	7.032576E-4	45.0	42
ACCCGTG	25	3.890216E-5	45.0	39
CGCGTAA	20	7.032576E-4	45.0	31
CCCTCGT	290	0.0	41.12069	14
CGGGTAC	50	1.0804797E-9	40.5	6
TAATACG	50	1.0804797E-9	40.5	21
CTCGTAG	45	1.927583E-8	40.0	1
TACGGCT	485	0.0	39.896908	7
CGAATAT	150	0.0	39.000004	14
GACCGAT	370	0.0	38.918922	9
ACGTAAG	35	6.2469735E-6	38.571426	1
TAGTAGG	275	0.0	37.636364	2
GCACCAC	305	0.0	37.62295	29
CGTAGTT	30	1.13975024E-4	37.500004	37
ACCAGTA	30	1.13975024E-4	37.500004	26
TGTACCG	30	1.13975024E-4	37.500004	1
CAATACG	30	1.13975024E-4	37.500004	1
CGTATGG	90	0.0	37.5	2