##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551827_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925787
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.170348039019775	28.0	25.0	31.0	16.0	33.0
2	27.489694713794858	31.0	25.0	33.0	16.0	34.0
3	27.41144561329982	31.0	25.0	33.0	16.0	34.0
4	31.766165435461936	35.0	28.0	37.0	19.0	37.0
5	33.08691308043859	35.0	32.0	35.0	28.0	37.0
6	32.69348997123529	35.0	32.0	35.0	28.0	37.0
7	34.09198660166972	35.0	33.0	37.0	31.0	37.0
8	34.02090329633058	35.0	35.0	37.0	30.0	37.0
9	36.398342167258775	39.0	37.0	39.0	32.0	39.0
10	35.36642013767746	37.0	34.0	39.0	31.0	39.0
11	35.850364068624856	37.0	35.0	39.0	31.0	39.0
12	35.88397331135564	37.0	35.0	39.0	31.0	39.0
13	36.045272832735826	37.0	35.0	39.0	32.0	39.0
14	36.98007317017846	39.0	36.0	40.0	32.0	41.0
15	37.166747858848744	39.0	36.0	40.0	32.0	41.0
16	36.72117128453953	38.0	35.0	40.0	31.0	41.0
17	36.879450672778944	38.0	35.0	40.0	32.0	41.0
18	36.672322035198164	38.0	35.0	40.0	31.0	41.0
19	36.28799821125162	38.0	34.0	40.0	30.0	41.0
20	36.77197886770931	38.0	35.0	40.0	32.0	41.0
21	36.85040835526962	39.0	35.0	40.0	32.0	41.0
22	37.250692653925796	39.0	35.0	40.0	32.0	41.0
23	37.60583157897011	39.0	36.0	40.0	33.0	41.0
24	37.674983554532524	39.0	36.0	41.0	33.0	41.0
25	36.5749864709701	38.0	35.0	40.0	31.0	41.0
26	36.907683948899695	39.0	35.0	40.0	32.0	41.0
27	37.21029567276274	39.0	35.0	40.0	32.0	41.0
28	37.26318364807456	39.0	35.0	40.0	32.0	41.0
29	37.37431828271514	39.0	35.0	41.0	33.0	41.0
30	36.93465667588765	39.0	35.0	40.0	31.0	41.0
31	37.1190349400024	39.0	35.0	40.0	32.0	41.0
32	37.090907519764265	39.0	35.0	40.0	32.0	41.0
33	37.237604330153694	39.0	35.0	41.0	33.0	41.0
34	37.25334553196362	40.0	35.0	41.0	33.0	41.0
35	37.19421421990155	40.0	35.0	41.0	32.0	41.0
36	37.0734553412394	39.0	35.0	41.0	32.0	41.0
37	37.13232957472939	40.0	35.0	41.0	32.0	41.0
38	37.02391154768861	39.0	35.0	41.0	32.0	41.0
39	36.9312930512094	39.0	35.0	41.0	32.0	41.0
40	36.82124721993288	39.0	35.0	41.0	31.0	41.0
41	36.65120594694028	39.0	35.0	40.0	31.0	41.0
42	36.761141601685914	39.0	35.0	41.0	32.0	41.0
43	36.75316568497937	39.0	35.0	41.0	32.0	41.0
44	36.77008102295668	39.0	35.0	41.0	32.0	41.0
45	36.78137735785877	39.0	35.0	41.0	32.0	41.0
46	36.57190152810528	39.0	35.0	40.0	31.0	41.0
47	36.53325008884333	39.0	35.0	40.0	31.0	41.0
48	36.52311708848796	39.0	35.0	40.0	31.0	41.0
49	36.45956791356975	39.0	35.0	40.0	31.0	41.0
50	36.35743210911365	38.0	35.0	40.0	31.0	41.0
51	35.41342987101785	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	15.0
9	28.0
10	42.0
11	53.0
12	53.0
13	55.0
14	92.0
15	131.0
16	185.0
17	278.0
18	413.0
19	848.0
20	1365.0
21	1981.0
22	2748.0
23	3726.0
24	4876.0
25	6237.0
26	7758.0
27	9009.0
28	10676.0
29	12654.0
30	16780.0
31	23353.0
32	34304.0
33	55416.0
34	74059.0
35	79701.0
36	117191.0
37	182419.0
38	214815.0
39	64525.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7763621653793	18.913313753595588	21.360528933761223	16.949795147263895
2	30.49859200874499	24.900976142460415	25.482535399611354	19.117896449183235
3	34.130744976976345	22.71775257159584	25.54766917228261	17.603833279145203
4	30.948155461245406	24.617865664564313	24.89082261902576	19.543156255164526
5	27.83880093369209	28.848860483026872	23.254593119151597	20.057745464129436
6	27.023710637544056	35.58064652020389	22.71580827987431	14.679834562377739
7	79.30981964533959	6.595901649083428	9.644227019822054	4.45005168575493
8	79.35194596597273	7.867036370136975	8.012102135804456	4.768915528085834
9	72.215963283131	9.313265362334965	9.948184625621229	8.522586728912806
10	43.91982173005238	24.806569977759462	16.91631012317088	14.35729816901728
11	33.5751096094458	25.831427747419223	22.493510926379397	18.099951716755584
12	31.313250240066022	23.42450261237196	25.039884984343054	20.222362163218968
13	27.764377767240195	23.956806479244147	26.87745669360231	21.40135905991335
14	21.841417086219618	27.4653889069516	26.79612048991831	23.897073516910478
15	21.287185929376847	26.884045682214158	30.776517708717016	21.05225067969198
16	24.906917033831757	25.78530482713626	28.280371186892882	21.0274069521391
17	24.000877091598824	27.52274551273673	26.854989322597962	21.621388073066484
18	24.265516798140393	27.107855262603604	26.312855980911376	22.31377195834463
19	26.040979188517444	27.339009945052155	25.161403216938673	21.45860764949173
20	28.391087798813334	26.831549805732852	26.96527386969141	17.812088525762405
21	28.147619268795086	26.693937158331238	25.592603914291303	19.565839658582373
22	25.520665120594693	25.28054509298575	27.022414443063035	22.176375343356515
23	25.825270823634376	26.557188640583636	26.959549010733568	20.65799152504842
24	24.797496616392323	25.650284568696684	27.4068441228922	22.14537469201879
25	23.84587383491019	27.99672062796302	25.931774803491514	22.225630733635274
26	23.798130671525954	27.944440783895214	26.28185532957365	21.975573215005177
27	25.3454628332435	27.903178592916085	25.8301315529382	20.921227020902215
28	22.429457315775657	29.16394375812147	26.946263017303117	21.460335908799756
29	24.90464869348997	26.159256934910513	26.464402718983955	22.471691652615558
30	24.091502689063468	27.18767923939308	26.40024109217347	22.320576979369985
31	25.540108037809993	27.308657390954938	24.894495170055315	22.256739401179754
32	27.441301292845978	28.06369067614905	24.088694267687924	20.406313763317048
33	25.506731029923728	27.517884783432905	24.223390477507245	22.751993709136116
34	24.385414787634737	28.00849439449895	25.23215383236101	22.373936985505306
35	25.083091467043715	27.238230824152854	24.970322547194982	22.70835516160845
36	24.02334446260317	29.358372930274456	24.096687466987547	22.521595140134824
37	25.02800320160037	28.875972550921542	26.075220326057725	20.02080392142037
38	25.011044657140356	26.823232557812975	24.846427958050825	23.319294826995844
39	25.49646949028232	26.781538302006837	25.07174976533479	22.650242442376054
40	23.90711902413838	26.82215239574546	27.423478618731956	21.847249961384204
41	21.476322307399002	28.52113931174233	26.817291666441633	23.185246714417033
42	22.565125671455746	26.911697831142583	26.881777341872375	23.641399155529296
43	23.94406056684745	25.88543585079505	26.280991199919633	23.88951238243786
44	23.737533579538276	26.386955098743016	26.16876236110466	23.706748960614053
45	23.938335707889614	25.75452020821204	25.6317057811354	24.67543830276295
46	23.407760100325454	28.571906928915613	24.748133209906815	23.272199760852118
47	22.70133410816959	27.268259329629817	27.940012119418395	22.090394442782195
48	23.496981487102325	26.321713309865014	27.155058344954075	23.026246858078586
49	22.58370445901703	26.609468484651437	28.06239448166803	22.744432574663502
50	22.277046448049063	26.686808088685627	27.370010596389882	23.666134866875428
51	21.66427050714689	26.220286091725203	25.682581414515433	26.432861986612473
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	297.0
1	923.0
2	1549.0
3	3171.5
4	4794.0
5	3449.5
6	2105.0
7	2023.5
8	1942.0
9	1972.0
10	2002.0
11	2000.0
12	1998.0
13	1963.0
14	1928.0
15	2061.5
16	2195.0
17	2129.0
18	2063.0
19	2208.5
20	2354.0
21	2466.0
22	2578.0
23	2972.0
24	3366.0
25	4227.0
26	6428.5
27	7769.0
28	9404.5
29	11040.0
30	13507.0
31	15974.0
32	17129.5
33	18285.0
34	21018.5
35	23752.0
36	25323.5
37	26895.0
38	29988.5
39	33082.0
40	36302.0
41	39522.0
42	41052.0
43	42582.0
44	47821.0
45	53060.0
46	55450.5
47	57841.0
48	67830.0
49	77819.0
50	71766.5
51	65714.0
52	66089.5
53	66465.0
54	63810.5
55	61156.0
56	59158.5
57	57161.0
58	56108.5
59	55056.0
60	53883.0
61	52710.0
62	48007.0
63	43304.0
64	36671.0
65	30038.0
66	23878.0
67	17718.0
68	15192.0
69	12666.0
70	10620.5
71	8575.0
72	7127.5
73	5680.0
74	4490.5
75	2757.5
76	2214.0
77	1731.0
78	1248.0
79	880.5
80	513.0
81	387.0
82	261.0
83	165.5
84	70.0
85	51.0
86	32.0
87	21.5
88	11.0
89	9.5
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	925787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.336675603404363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18476365375798	17.81073852153326
2	8.718726674118951	4.24369645485563
3	3.0967892621975057	2.260966670586199
4	1.5596398393898663	1.5182579531750742
5	1.0991052118347013	1.337428349721608
6	0.8269772641579212	1.2075526445521296
7	0.6896540534778728	1.1748720884644712
8	0.5825828023011238	1.1342502937379764
9	0.5301312290592168	1.1611468573963388
>10	8.909783525685297	51.580179667794724
>50	0.7396496864613542	11.04397046907354
>100	0.05235270010290296	2.237172557000746
>500	0.006264425653338815	1.0634845790337466
>1k	0.0035796718019078947	2.2262828930745355
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC	4395	0.47473122867355017	RNA PCR Primer, Index 34 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG	4278	0.4620933324836058	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC	4114	0.44437867457633345	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2162	0.23353103899709113	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1732	0.18708407009387687	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC	1525	0.16472471529628305	TruSeq Adapter, Index 18 (95% over 23bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1188	0.1283232536209733	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCT	1050	0.11341701708924407	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGCAACT	955	0.10315547744783628	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04353053132091939	0.0
2	0.0	0.0	0.0	0.3324738843816126	0.0
3	0.0	0.0	0.0	0.4228834494327529	0.0
4	0.0	0.0	0.0	0.6771536001261629	0.0
5	0.0	0.0	0.0	1.5351263303546063	0.0
6	0.0	0.0	0.0	1.8750533330020835	0.0
7	0.0	0.0	0.0	2.2240536970167004	0.0
8	0.0	0.0	0.0	2.638727914736327	0.0
9	0.0	0.0	0.0	2.7992399979692952	0.0
10	1.0801620675166102E-4	0.0	0.0	3.623727704104724	0.0
11	1.0801620675166102E-4	0.0	0.0	4.1593800733862105	0.0
12	1.0801620675166102E-4	0.0	0.0	5.023509727399499	0.0
13	1.0801620675166102E-4	0.0	0.0	5.212754121628409	0.0
14	0.008317247919877898	0.0	0.0	5.302623605645791	0.0
15	0.008317247919877898	0.0	0.0	5.532698126026829	0.0
16	0.008317247919877898	0.0	0.0	5.805547064281525	0.0
17	0.008317247919877898	0.0	0.0	6.106804264911907	0.0
18	0.008317247919877898	0.0	0.0	6.389374661774252	0.0
19	0.008317247919877898	0.0	0.0	6.71439542789	0.0
20	0.008317247919877898	0.0	0.0	6.938529056899697	0.0
21	0.008317247919877898	0.0	0.0	7.203924876888529	0.0
22	0.008317247919877898	0.0	0.0	7.516631795434587	0.0
23	0.008317247919877898	0.0	0.0	7.777058869912842	0.0
24	0.008317247919877898	0.0	0.0	8.011454038563945	0.0
25	0.008317247919877898	0.0	0.0	8.233103294818354	0.0
26	0.008317247919877898	0.0	0.0	8.428288580418606	0.0
27	0.008317247919877898	0.0	0.0	8.631035000491474	0.0
28	0.008533280333381221	0.0	0.0	8.837237939180394	0.0
29	0.008533280333381221	0.0	0.0	9.056618855093019	0.0
30	0.008533280333381221	0.0	0.0	9.295658720634444	0.0
31	0.008533280333381221	0.0	0.0	9.532862310661091	0.0
32	0.008533280333381221	0.0	0.0	9.775250678611819	0.0
33	0.008533280333381221	0.0	0.0	10.007161474507635	0.0
34	0.008533280333381221	0.0	0.0	10.24026044867772	0.0
35	0.008533280333381221	0.0	0.0	10.478652216978636	0.0
36	0.008533280333381221	0.0	0.0	10.720500503895604	0.0
37	0.008533280333381221	0.0	0.0	10.977255027344302	0.0
38	0.008533280333381221	0.0	0.0	11.225692302873123	0.0
39	0.008533280333381221	0.0	0.0	11.482446826321821	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	25	3.890216E-5	45.0	1
TATGCGC	20	7.032576E-4	45.0	18
GTATCAC	20	7.032576E-4	45.0	42
ACCCGTG	25	3.890216E-5	45.0	39
CGCGTAA	20	7.032576E-4	45.0	31
CCCTCGT	290	0.0	41.12069	14
CGGGTAC	50	1.0804797E-9	40.5	6
TAATACG	50	1.0804797E-9	40.5	21
CTCGTAG	45	1.927583E-8	40.0	1
TACGGCT	485	0.0	39.896908	7
CGAATAT	150	0.0	39.000004	14
GACCGAT	370	0.0	38.918922	9
ACGTAAG	35	6.2469735E-6	38.571426	1
TAGTAGG	275	0.0	37.636364	2
GCACCAC	305	0.0	37.62295	29
CGTAGTT	30	1.13975024E-4	37.500004	37
ACCAGTA	30	1.13975024E-4	37.500004	26
TGTACCG	30	1.13975024E-4	37.500004	1
CAATACG	30	1.13975024E-4	37.500004	1
CGTATGG	90	0.0	37.5	2
>>END_MODULE