FastQCFastQC Report
Sat 18 Jun 2016
SRR3551824_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551824_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340458
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG44781.3152870544971773No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC43631.281509026076638No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC37671.1064507222623643No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC20990.6165224491714103No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15090.4432264772747299No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC10530.3092892515376346No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCCTGTT10380.30488342174365124No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT9680.284322882705062No Hit
GAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT8410.2470201904493359No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA6910.20296189250950192No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC6580.19326906696273843No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT6350.18651346127863055Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC4580.13452466970962645No Hit
TAACGAGGGATTGCTTTTAAAACTATCTTTCTTTGAGCTTCTTTATGTTGC3490.10250897320668041No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG207.026284E-445.0000041
TTCTATC207.026284E-445.00000422
CGGAAAT207.026284E-445.00000439
CTCACAT207.026284E-445.0000047
AGATCGA207.026284E-445.00000413
CCCTCGC207.026284E-445.00000415
CCGATCG453.8380676E-1045.00000440
TAGCGTA207.026284E-445.00000412
TAAACGG207.026284E-445.0000042
TATGAAC207.026284E-445.00000419
TATCTCC207.026284E-445.00000413
CAGATCG207.026284E-445.00000414
AACGAGG800.045.0000042
CTACCGG406.7902874E-945.0000042
GCTAGCG406.7902874E-945.0000041
CGTAAGT207.026284E-445.00000440
ATGCGGA207.026284E-445.00000445
CGTCGGA207.026284E-445.00000431
TGCTACG207.026284E-445.0000041
TCCCCCG207.026284E-445.00000414