##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551824_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340458
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.469511657825635	28.0	25.0	31.0	16.0	33.0
2	27.59985372645084	31.0	25.0	33.0	16.0	34.0
3	27.410273807635598	30.0	25.0	33.0	16.0	34.0
4	31.655872383671408	35.0	28.0	37.0	19.0	37.0
5	33.207156242473374	35.0	32.0	35.0	28.0	37.0
6	32.9917493494058	35.0	32.0	35.0	28.0	37.0
7	33.939173113864264	35.0	33.0	37.0	31.0	37.0
8	33.81886165107003	35.0	35.0	37.0	29.0	37.0
9	36.01962944034213	39.0	35.0	39.0	30.0	39.0
10	35.346527324956384	37.0	34.0	39.0	31.0	39.0
11	35.93486714954561	37.0	35.0	39.0	31.0	39.0
12	35.865921787709496	37.0	35.0	39.0	31.0	39.0
13	35.93671466083922	37.0	35.0	39.0	31.0	39.0
14	36.78986835380576	38.0	36.0	40.0	31.0	41.0
15	37.04518031592737	39.0	36.0	40.0	32.0	41.0
16	36.543946683585055	38.0	34.0	40.0	32.0	41.0
17	36.7754877253582	38.0	35.0	40.0	32.0	41.0
18	36.601848098737584	38.0	35.0	40.0	31.0	41.0
19	36.153710589852494	38.0	34.0	40.0	30.0	41.0
20	36.61135294221314	38.0	35.0	40.0	31.0	41.0
21	36.747960100805386	38.0	35.0	40.0	32.0	41.0
22	37.14873787662502	39.0	35.0	40.0	32.0	41.0
23	37.48766955101657	39.0	35.0	40.0	33.0	41.0
24	37.52091006820225	39.0	35.0	41.0	33.0	41.0
25	36.56705966668429	38.0	35.0	40.0	31.0	41.0
26	36.83908734704428	39.0	35.0	40.0	32.0	41.0
27	37.140205252924005	39.0	35.0	40.0	33.0	41.0
28	37.2004535067468	39.0	35.0	40.0	32.0	41.0
29	37.31108389287372	39.0	35.0	41.0	33.0	41.0
30	36.93234114046373	39.0	35.0	40.0	32.0	41.0
31	37.093353658894785	39.0	35.0	40.0	32.0	41.0
32	36.987925089144625	39.0	35.0	40.0	32.0	41.0
33	37.0562125137315	39.0	35.0	41.0	32.0	41.0
34	37.05382455398316	39.0	35.0	41.0	32.0	41.0
35	37.02110098749332	40.0	35.0	41.0	32.0	41.0
36	36.864506047735695	39.0	35.0	41.0	32.0	41.0
37	36.95565972895335	39.0	35.0	41.0	32.0	41.0
38	36.83291037367311	39.0	35.0	41.0	32.0	41.0
39	36.75649859894612	39.0	35.0	41.0	32.0	41.0
40	36.649613168144086	39.0	35.0	40.0	31.0	41.0
41	36.44198109605296	39.0	35.0	40.0	31.0	41.0
42	36.62158327899476	39.0	35.0	40.0	31.0	41.0
43	36.585094196640995	39.0	35.0	40.0	31.0	41.0
44	36.67939070311169	39.0	35.0	40.0	32.0	41.0
45	36.69803911201969	39.0	35.0	40.0	32.0	41.0
46	36.54076861169366	39.0	35.0	40.0	32.0	41.0
47	36.47618795857345	39.0	35.0	40.0	31.0	41.0
48	36.52692549448096	39.0	35.0	40.0	32.0	41.0
49	36.53337562929936	39.0	35.0	40.0	32.0	41.0
50	36.38454963607846	39.0	35.0	40.0	31.0	41.0
51	35.51562894688919	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	16.0
10	19.0
11	19.0
12	10.0
13	16.0
14	24.0
15	27.0
16	51.0
17	87.0
18	114.0
19	266.0
20	470.0
21	763.0
22	1107.0
23	1519.0
24	2047.0
25	2642.0
26	3109.0
27	3385.0
28	3899.0
29	4585.0
30	6188.0
31	8708.0
32	12969.0
33	21439.0
34	29314.0
35	29398.0
36	41948.0
37	65935.0
38	78064.0
39	22314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14939875109411	18.406088269331313	20.667160119603594	18.777352859970982
2	28.664916083628523	27.93619183570367	24.07668493617421	19.322207144493593
3	30.556779397164995	26.172391308178984	25.916853180127948	17.353976114528077
4	28.310099924219728	23.547985360896202	29.28848786047031	18.85342685441376
5	28.357976607981012	27.030940674033214	24.630350880284794	19.98073183770098
6	25.066821751875413	34.98875044792603	24.419752216132387	15.52467558406617
7	71.41497629663571	6.948287307098086	15.625715947341522	6.0110204489246835
8	70.86718479225044	10.439466835850531	11.545330114140363	7.148018257758666
9	65.0570701819314	8.629551956482151	13.430437821992728	12.882940039593723
10	41.97669022316997	22.83923420803741	20.139929154256915	15.044146414535714
11	33.20850148917047	23.173489828407615	24.48437105311081	19.133637629311103
12	29.996651569356576	20.726198238842972	27.961745648508774	21.315404543291685
13	24.501700650300478	24.85152353594276	31.96811354117101	18.678662272585754
14	19.36332822257077	30.10855964612375	27.40514248453554	23.12296964676994
15	17.843023221660236	24.597160295836783	37.554999441928224	20.004817040574753
16	19.210299067726417	24.177725299449566	32.40311580283031	24.208859829993713
17	19.498146614266666	25.84430384952035	29.457378002573005	25.20017153363998
18	20.53968477756434	25.376111003413047	31.231165077630724	22.85303914139189
19	23.21813557031998	26.45642046889778	26.87967385110645	23.44577010967579
20	25.929189503551097	26.02259309518355	29.98284663600209	18.065370765263264
21	24.076391214187947	28.81324568669263	26.61855500531637	20.491808093803055
22	21.526590651416623	25.196059425832264	27.355503468856657	25.92184645389446
23	22.342550329262348	27.611629040880224	28.38411786475865	21.661702765098777
24	23.620534691503796	24.19006162287272	29.466483384147235	22.722920301476247
25	20.296189250950192	29.318447503069393	26.95310434767284	23.432258898307573
26	19.836808064430855	28.790041649777653	29.043817445911095	22.329332839880397
27	22.38249651939446	27.793149228392345	27.407785982411927	22.416568269801267
28	19.63267128397629	27.37665145186778	30.503321995664663	22.487355268491267
29	20.63573186707318	25.436911454570023	29.35898113717404	24.56837554118276
30	19.752803576358904	29.0335371763918	29.310223287453958	21.903435959795335
31	25.39432176656151	26.233485481322216	24.33809750395056	24.034095248165706
32	25.556162580993835	27.757021424081678	24.203572834240937	22.48324316068355
33	22.834240934270895	25.325590821775375	25.86222089068255	25.97794735327118
34	21.75305030282737	26.0707635009311	28.422008000986903	23.754178195254628
35	22.963772330214006	24.229126647046037	28.19026135382338	24.616839668916576
36	23.5838194432206	27.171046061481885	27.75907747798554	21.486057017311975
37	21.467846254163508	26.352149163773504	30.680730075369063	21.499274506693926
38	22.029736413889527	26.220561713926532	25.8669204424628	25.882781429721142
39	22.111978570043885	24.946102015520268	29.95758654518325	22.984332869252594
40	23.25661315052077	23.251032432781724	29.51670984379865	23.97564457289886
41	20.020384305846832	24.791604250744584	30.08094977941479	25.107061663993797
42	22.731144517091685	23.53564903747305	29.645947517755495	24.087258927679773
43	23.174664716352677	23.637276844720933	28.436106656327652	24.751951782598734
44	21.71927227440683	24.572193927004214	28.249299473062756	25.459234325526204
45	21.507792444295625	24.401247730997653	26.53690029313454	27.55405953157218
46	23.548572804868737	27.10936444436612	26.427048270271225	22.915014480493923
47	19.631790118017495	24.469097509824998	32.674808640125946	23.224303732031558
48	21.50603011237803	23.36235306557637	29.852140352113913	25.279476469931677
49	20.77583725452185	22.090536865046495	32.91536694687744	24.218258933554214
50	20.938559234912972	23.28627907113359	30.70452155625657	25.070640137696863
51	19.622391014456998	23.439308225977946	27.512644731508733	29.425656028056324
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	311.5
2	504.0
3	1606.0
4	2708.0
5	1857.5
6	1007.0
7	1015.5
8	1024.0
9	964.5
10	905.0
11	918.0
12	931.0
13	925.5
14	920.0
15	851.5
16	783.0
17	809.0
18	835.0
19	842.0
20	849.0
21	952.0
22	1055.0
23	1214.5
24	1374.0
25	1529.0
26	2255.0
27	2826.0
28	3067.5
29	3309.0
30	4037.0
31	4765.0
32	5526.0
33	6287.0
34	6815.0
35	7343.0
36	8383.0
37	9423.0
38	10214.5
39	11006.0
40	12595.0
41	14184.0
42	16610.5
43	19037.0
44	20922.5
45	22808.0
46	33264.5
47	43721.0
48	38337.0
49	32953.0
50	31852.5
51	30752.0
52	27237.5
53	23723.0
54	21272.0
55	18821.0
56	17151.5
57	15482.0
58	14731.5
59	13981.0
60	13373.5
61	12766.0
62	11819.0
63	10872.0
64	8803.5
65	6735.0
66	5820.0
67	4905.0
68	4162.0
69	3419.0
70	2873.0
71	2327.0
72	2026.0
73	1725.0
74	1407.5
75	853.5
76	617.0
77	513.0
78	409.0
79	343.0
80	277.0
81	185.5
82	94.0
83	81.0
84	68.0
85	44.0
86	20.0
87	15.0
88	10.0
89	7.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.467666910672907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72899334285867	21.431537505759064
2	11.210998973817714	6.607239669927123
3	4.071008724153294	3.598893872213782
4	1.9219998801957214	2.26547409087843
5	1.1489998952663303	1.6929173097053134
6	0.8309999239397863	1.4692577376871285
7	0.5949999455375946	1.2273282144879252
8	0.48399995569777704	1.140987958342603
9	0.4289999607321202	1.1377465152791286
>10	6.010999449792015	41.39470130376473
>50	0.513999952951771	9.280546167366195
>100	0.04199999615559218	2.241015863428587
>500	0.004999999542332403	1.1177085036140209
>1k	0.006999999359265365	5.394645287545974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	4478	1.3152870544971773	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	4363	1.281509026076638	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	3767	1.1064507222623643	No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2099	0.6165224491714103	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1509	0.4432264772747299	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1053	0.3092892515376346	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGCCTGTT	1038	0.30488342174365124	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	968	0.284322882705062	No Hit
GAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	841	0.2470201904493359	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTA	691	0.20296189250950192	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	658	0.19326906696273843	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	635	0.18651346127863055	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	458	0.13452466970962645	No Hit
TAACGAGGGATTGCTTTTAAAACTATCTTTCTTTGAGCTTCTTTATGTTGC	349	0.10250897320668041	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9372198626555993E-4	0.0	0.0	0.1920941790176762	0.0
2	2.9372198626555993E-4	0.0	0.0	1.3752063396953516	0.0
3	5.874439725311199E-4	0.0	0.0	1.6903700309582974	0.0
4	5.874439725311199E-4	0.0	0.0	2.3870785823802056	0.0
5	5.874439725311199E-4	0.0	0.0	4.86814820036539	0.0
6	5.874439725311199E-4	0.0	0.0	5.667659446980244	0.0
7	5.874439725311199E-4	0.0	0.0	6.736513755000617	0.0
8	5.874439725311199E-4	0.0	0.0	8.03682098819825	0.0
9	5.874439725311199E-4	0.0	0.0	8.499433116566507	0.0
10	5.874439725311199E-4	0.0	0.0	10.734363710061153	0.0
11	5.874439725311199E-4	0.0	0.0	12.00001174887945	0.0
12	5.874439725311199E-4	0.0	0.0	14.174141891217126	0.0
13	5.874439725311199E-4	0.0	0.0	14.679637429580154	0.0
14	5.874439725311199E-4	0.0	0.0	14.888473761814966	0.0
15	5.874439725311199E-4	0.0	0.0	15.431859436406253	0.0
16	5.874439725311199E-4	0.0	0.0	16.080103860094344	0.0
17	5.874439725311199E-4	0.0	0.0	16.821458153428615	0.0
18	5.874439725311199E-4	0.0	0.0	17.586897649636665	0.0
19	5.874439725311199E-4	0.0	0.0	18.377890958649818	0.0
20	5.874439725311199E-4	0.0	0.0	18.895135376463468	0.0
21	5.874439725311199E-4	0.0	0.0	19.483166792967122	0.0
22	5.874439725311199E-4	0.0	0.0	20.15667130747405	0.0
23	5.874439725311199E-4	0.0	0.0	20.77378120061799	0.0
24	5.874439725311199E-4	0.0	0.0	21.261359697818822	0.0
25	5.874439725311199E-4	0.0	0.0	21.677857474343384	0.0
26	5.874439725311199E-4	0.0	0.0	22.089949421073964	0.0
27	5.874439725311199E-4	0.0	0.0	22.484418048628612	0.0
28	5.874439725311199E-4	0.0	0.0	22.908258874809814	0.0
29	8.811659587966798E-4	0.0	0.0	23.292153510858903	0.0
30	8.811659587966798E-4	0.0	0.0	23.75065353141944	0.0
31	8.811659587966798E-4	0.0	0.0	24.171263415751724	0.0
32	8.811659587966798E-4	0.0	0.0	24.62594505049081	0.0
33	8.811659587966798E-4	0.0	0.0	25.028637893660893	0.0
34	8.811659587966798E-4	0.0	0.0	25.418113247449025	0.0
35	8.811659587966798E-4	0.0	0.0	25.871913716229315	0.0
36	8.811659587966798E-4	0.0	0.0	26.317196247407903	0.0
37	8.811659587966798E-4	0.0	0.0	26.746617791328152	0.0
38	8.811659587966798E-4	0.0	0.0	27.167815119632966	0.0
39	8.811659587966798E-4	0.0	0.0	27.562577469173878	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	20	7.026284E-4	45.000004	1
TTCTATC	20	7.026284E-4	45.000004	22
CGGAAAT	20	7.026284E-4	45.000004	39
CTCACAT	20	7.026284E-4	45.000004	7
AGATCGA	20	7.026284E-4	45.000004	13
CCCTCGC	20	7.026284E-4	45.000004	15
CCGATCG	45	3.8380676E-10	45.000004	40
TAGCGTA	20	7.026284E-4	45.000004	12
TAAACGG	20	7.026284E-4	45.000004	2
TATGAAC	20	7.026284E-4	45.000004	19
TATCTCC	20	7.026284E-4	45.000004	13
CAGATCG	20	7.026284E-4	45.000004	14
AACGAGG	80	0.0	45.000004	2
CTACCGG	40	6.7902874E-9	45.000004	2
GCTAGCG	40	6.7902874E-9	45.000004	1
CGTAAGT	20	7.026284E-4	45.000004	40
ATGCGGA	20	7.026284E-4	45.000004	45
CGTCGGA	20	7.026284E-4	45.000004	31
TGCTACG	20	7.026284E-4	45.000004	1
TCCCCCG	20	7.026284E-4	45.000004	14
>>END_MODULE