##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551823_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470263
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.696474100662822	30.0	25.0	31.0	16.0	33.0
2	27.695385348198773	31.0	25.0	33.0	16.0	34.0
3	27.504037102642563	30.0	25.0	33.0	16.0	34.0
4	31.684514835315557	35.0	28.0	35.0	19.0	37.0
5	33.27012756691468	35.0	32.0	35.0	28.0	37.0
6	33.081499076048935	35.0	32.0	35.0	28.0	37.0
7	34.08475470109279	35.0	33.0	37.0	31.0	37.0
8	34.18134533229278	35.0	35.0	37.0	31.0	37.0
9	36.39157450192764	39.0	37.0	39.0	32.0	39.0
10	35.47421123924272	37.0	34.0	39.0	31.0	39.0
11	36.02442675694239	37.0	35.0	39.0	31.0	39.0
12	36.069327163736034	37.0	35.0	39.0	32.0	39.0
13	36.120334791382696	37.0	35.0	39.0	32.0	39.0
14	37.0308359364866	39.0	36.0	40.0	32.0	41.0
15	37.23086230471034	39.0	36.0	40.0	32.0	41.0
16	36.81750212115348	38.0	35.0	40.0	32.0	41.0
17	36.9235597952635	38.0	35.0	40.0	32.0	41.0
18	36.783774185934256	38.0	35.0	40.0	32.0	41.0
19	36.37060325817681	38.0	34.0	40.0	30.0	41.0
20	36.629026736102986	38.0	35.0	40.0	31.0	41.0
21	36.92708760842337	39.0	35.0	40.0	32.0	41.0
22	37.29306792156729	39.0	35.0	40.0	32.0	41.0
23	37.628078330636264	39.0	36.0	40.0	33.0	41.0
24	37.67259171995245	39.0	36.0	41.0	33.0	41.0
25	36.776905688944275	38.0	35.0	40.0	31.0	41.0
26	37.05866504487914	39.0	35.0	40.0	32.0	41.0
27	37.3008656857971	39.0	35.0	40.0	33.0	41.0
28	37.31764565785529	39.0	35.0	40.0	32.0	41.0
29	37.425797904576804	39.0	36.0	41.0	33.0	41.0
30	37.05016554566274	39.0	35.0	40.0	32.0	41.0
31	37.21959414200139	39.0	35.0	40.0	33.0	41.0
32	37.14468712188712	39.0	35.0	40.0	33.0	41.0
33	37.1384289216885	40.0	35.0	41.0	32.0	41.0
34	37.16776357059773	40.0	36.0	41.0	33.0	41.0
35	37.0606979498706	40.0	35.0	41.0	32.0	41.0
36	36.95192264753978	40.0	35.0	41.0	31.0	41.0
37	37.00108875246405	40.0	35.0	41.0	32.0	41.0
38	36.89504596364162	40.0	35.0	41.0	31.0	41.0
39	36.87530807229146	40.0	35.0	41.0	32.0	41.0
40	36.68936105966236	39.0	35.0	41.0	31.0	41.0
41	36.56829050977857	39.0	35.0	41.0	31.0	41.0
42	36.76807020752217	39.0	35.0	41.0	31.0	41.0
43	36.733079149327075	39.0	35.0	41.0	32.0	41.0
44	36.845248297229425	39.0	35.0	41.0	32.0	41.0
45	36.85831332679798	39.0	35.0	41.0	32.0	41.0
46	36.6872601076419	39.0	35.0	41.0	32.0	41.0
47	36.55421966006256	39.0	35.0	41.0	31.0	41.0
48	36.655537433308595	39.0	35.0	41.0	32.0	41.0
49	36.6628482359871	39.0	35.0	41.0	32.0	41.0
50	36.52479144648846	39.0	35.0	41.0	31.0	41.0
51	35.67011650927247	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	11.0
10	17.0
11	17.0
12	14.0
13	10.0
14	24.0
15	40.0
16	51.0
17	74.0
18	177.0
19	447.0
20	792.0
21	1117.0
22	1603.0
23	2118.0
24	2955.0
25	3954.0
26	4543.0
27	4750.0
28	5065.0
29	6093.0
30	7845.0
31	11065.0
32	16172.0
33	25724.0
34	35552.0
35	38078.0
36	56455.0
37	93116.0
38	115987.0
39	36389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.266561477301	18.13708499286569	22.1110314866362	17.485322043197105
2	27.784877823685893	30.178857362794865	24.20517880420105	17.831086009318188
3	28.901061746299412	28.29055230796384	26.758856214501247	16.049529731235502
4	26.88261674850031	22.515911309203574	32.74337976834239	17.858092173953725
5	29.430340043762747	25.990775374630793	24.83780352696257	19.741081054643892
6	22.444249281784874	36.35986671288194	25.86318719525032	15.332696810082869
7	73.05890533595031	5.117987168882094	16.76848912204447	5.054618373123125
8	72.7716192853786	10.285308433791304	11.20862155857467	5.734450722255419
9	67.67298299036922	7.068597784643912	11.713870748921348	13.544548476065522
10	39.390085973168205	26.682303306872964	19.82103631372232	14.106574406236511
11	30.048930066792412	23.055821954948613	28.06982475763562	18.825423220623353
12	27.368302418008643	19.793392208189886	31.478342969784993	21.359962404016475
13	23.77393075789505	22.96906199296989	35.2285848557084	18.02842239342666
14	18.568333039171698	30.165885897891183	28.275241726438182	22.990539336498937
15	16.39401781556278	25.196113664056067	38.91949823821989	19.49037028216126
16	18.41884222233091	26.09390915296334	31.361387138686226	24.125861486019527
17	18.72122620746263	26.832857358541922	30.77490680746731	23.671009626528132
18	19.40892649432339	27.131626345257864	30.878678526696763	22.58076863372198
19	21.133706032581767	28.171470007208733	27.18287426397569	23.51194969623381
20	23.49855293739886	26.962146713647467	32.31255701596766	17.22674333298601
21	22.078283853928546	29.938140997697033	29.132208997943703	18.851366150430714
22	19.510571743896502	24.756572386090337	30.48294252365166	25.249913346361502
23	20.997399327610296	27.556707629560478	30.53419044236948	20.91170260045974
24	21.804181915226163	24.68639888743108	29.638734070084187	23.870685127258575
25	19.046788711848475	31.586792922258393	27.8933277761593	21.47309058973383
26	19.871008350646342	27.763613127122483	29.756115195114223	22.609263327116956
27	22.402570476520587	29.09095548661068	27.500781477598707	21.005692559270024
28	17.855965704297383	27.954782749227558	32.83651913929015	21.352732407184916
29	23.764361644441514	23.87089777422421	29.951750403497616	22.412990177836658
30	19.266027733417257	31.733094034614673	28.796864733138687	20.20401349882938
31	21.803331327363622	26.424575184524407	29.25937188339291	22.51272160471906
32	24.712767111169708	29.133910173668774	27.728101083861585	18.42522163129993
33	20.968054046352787	28.856405883516246	26.403523134926626	23.77201693520434
34	23.23295687732184	24.75168150588075	30.8072291462437	21.20813247055371
35	23.258049219266667	26.55131277604234	26.981922881451442	23.20871512323955
36	22.08721502648518	27.44634385439637	27.90225894871593	22.564182170402518
37	20.413683406944624	27.448257677087078	33.1210407793086	19.0170181366597
38	19.843789539045172	28.239304389246016	28.400916083127953	23.515989988580856
39	21.95324743813568	25.30690273315145	32.16349149305814	20.576358335654728
40	23.570640258748828	25.86999189815061	30.01809625677546	20.5412715863251
41	19.631993161273588	26.197468225227162	29.048426093483858	25.122112520015392
42	22.885066441544414	24.129051190504036	30.822327080803717	22.16355528714783
43	23.133863391336337	25.125089577534272	26.688257421910716	25.052789609218674
44	21.23131098980783	25.53209586975799	29.08351284281349	24.153080297620694
45	20.748602377818372	23.948088622749395	28.703725362190944	26.599583637241288
46	23.702481377441984	28.141912078985587	27.127373405945182	21.028233137627243
47	19.310258302269155	25.379415348432687	34.083906239699914	21.226420109598244
48	21.306800662607944	24.59793774972728	29.309131273351298	24.78613031431348
49	20.133840000170117	22.592889510763126	34.21553471142744	23.057735777639323
50	20.191467327856966	23.296963613977713	30.76618828187631	25.74538077628901
51	20.61144508498436	23.2588998071292	27.68408316197532	28.44557194591112
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	369.5
2	549.0
3	2241.5
4	3934.0
5	2757.5
6	1581.0
7	1532.5
8	1484.0
9	1484.5
10	1485.0
11	1456.0
12	1427.0
13	1351.5
14	1276.0
15	1248.5
16	1221.0
17	1230.0
18	1239.0
19	1310.0
20	1381.0
21	1464.0
22	1547.0
23	1678.0
24	1809.0
25	2342.5
26	4251.0
27	5626.0
28	5415.5
29	5205.0
30	6692.5
31	8180.0
32	8854.0
33	9528.0
34	11099.5
35	12671.0
36	13798.5
37	14926.0
38	16694.5
39	18463.0
40	20999.5
41	23536.0
42	26028.0
43	28520.0
44	49334.5
45	70149.0
46	59242.5
47	48336.0
48	46568.0
49	44800.0
50	38669.0
51	32538.0
52	28939.5
53	25341.0
54	23042.5
55	20744.0
56	18967.5
57	17191.0
58	16881.5
59	16572.0
60	15519.0
61	14466.0
62	12855.5
63	11245.0
64	9255.0
65	7265.0
66	5842.0
67	4419.0
68	3656.0
69	2893.0
70	2420.5
71	1948.0
72	1671.5
73	1395.0
74	1179.5
75	787.0
76	610.0
77	465.0
78	320.0
79	236.5
80	153.0
81	155.5
82	158.0
83	114.0
84	70.0
85	39.0
86	8.0
87	9.5
88	11.0
89	9.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.626358284482436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41094967357641	23.21720996380636
2	10.787327052763818	6.823277406051968
3	3.978372033275145	3.774642579399739
4	1.9993237139562012	2.5292531241696357
5	1.2548171015597773	1.984264761771265
6	0.8930847151212022	1.694701030729084
7	0.6617826253337706	1.4650842090675862
8	0.5636686162059026	1.426142848787704
9	0.4424715573985392	1.2594387604481223
>10	5.696216219407405	39.22849046744918
>50	0.26876775579639905	5.246170103895272
>100	0.033089497572923496	1.9787398637269866
>500	0.0027011834753406937	0.5478108743075791
>1k	0.005402366950681387	3.325735257199853
>5k	0.0020258876065055204	5.499038749189687
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	9273	1.9718753123252306	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	8969	1.9072306347724572	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	7506	1.5961281240497338	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	2766	0.5881815069439866	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	2201	0.4680359713607067	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2177	0.46293244418548773	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	1964	0.4176386405054193	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	1907	0.4055177634642742	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1570	0.3338557360455745	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	1503	0.3196083893480882	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	1484	0.3155680970010398	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	792	0.1684163967822261	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	666	0.14162287911232652	No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	560	0.11908230075510938	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	547	0.11631789020186577	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17224404216364034	0.0
2	0.0	0.0	0.0	1.3230894201755188	0.0
3	0.0	0.0	0.0	1.6952216100352355	0.0
4	0.0	0.0	0.0	2.591315923217434	0.0
5	0.0	0.0	0.0	5.743381894812052	0.0
6	0.0	0.0	0.0	6.558245067122015	0.0
7	0.0	0.0	0.0	7.773735122686666	0.0
8	0.0	0.0	0.0	9.004110465845708	0.0
9	0.0	0.0	0.0	9.35901825148906	0.0
10	0.0	0.0	0.0	12.22188434982127	0.0
11	0.0	0.0	0.0	13.598986099267856	0.0
12	0.0	0.0	0.0	16.261538755972722	0.0
13	0.0	0.0	0.0	16.82547850883442	0.0
14	0.0	0.0	0.0	17.030044889774445	0.0
15	0.0	0.0	0.0	17.647359031010307	0.0
16	0.0	0.0	0.0	18.349519311534184	0.0
17	0.0	0.0	0.0	19.183946004682486	0.0
18	0.0	0.0	0.0	19.9947688846454	0.0
19	0.0	0.0	0.0	20.95019170123952	0.0
20	0.0	0.0	0.0	21.544539970186896	0.0
21	0.0	0.0	0.0	22.184607336745607	0.0
22	0.0	0.0	0.0	22.936314360262237	0.0
23	0.0	0.0	0.0	23.646342578514577	0.0
24	0.0	0.0	0.0	24.161798823211694	0.0
25	0.0	0.0	0.0	24.63344979298818	0.0
26	0.0	0.0	0.0	25.0604448999815	0.0
27	0.0	0.0	0.0	25.54655586342111	0.0
28	0.0	0.0	0.0	25.981844202074157	0.0
29	0.0	0.0	0.0	26.428402829905817	0.0
30	0.0	0.0	0.0	26.942370545843495	0.0
31	0.0	0.0	0.0	27.43762532880537	0.0
32	0.0	0.0	0.0	27.894603657953102	0.0
33	0.0	0.0	0.0	28.330955231434324	0.0
34	0.0	0.0	0.0	28.762415924705962	0.0
35	0.0	0.0	0.0	29.251716592630082	0.0
36	0.0	0.0	0.0	29.72506873813164	0.0
37	0.0	0.0	0.0	30.21862234536844	0.0
38	0.0	0.0	0.0	30.676451262378713	0.0
39	0.0	0.0	0.0	31.152142524502246	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTTGCG	30	2.1627548E-6	45.000004	1
ACGGGCC	30	2.1627548E-6	45.000004	5
CCGATTT	40	6.7993824E-9	45.0	11
GCGAACC	20	7.029032E-4	45.0	33
GCGCCAA	20	7.029032E-4	45.0	40
CGATTTA	20	7.029032E-4	45.0	10
CGATTGC	25	3.8872782E-5	45.0	10
ACGGGTC	20	7.029032E-4	45.0	5
CGCTCGA	55	1.8189894E-12	45.0	41
CTACGCT	25	3.8872782E-5	45.0	38
CACTACG	20	7.029032E-4	45.0	2
ACTACGC	25	3.8872782E-5	45.0	37
AACTCGG	20	7.029032E-4	45.0	31
CGATATA	20	7.029032E-4	45.0	21
TACGGCT	945	0.0	42.619045	7
CGTTTTT	1420	0.0	42.306335	1
TACGGTT	70	0.0	41.785717	33
CCCGATT	60	3.6379788E-12	41.250004	40
CCTCGTC	115	0.0	41.08696	36
ACGGCTG	975	0.0	41.076923	8
>>END_MODULE