FastQCFastQC Report
Sat 18 Jun 2016
SRR3551821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536618
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC47370.8827508581523542No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC47040.8766012321614258No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG45030.8391444193075893No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC18910.35239220451047115No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14210.2648066222154307No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT12800.23853094752691859No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT11750.21896395573760105No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT9260.17256223235150517No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA8930.16641260636057678No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC7090.13212378265358224No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC7050.13137837344256062No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC5650.10528905105680392No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG207.0299226E-445.01
ACACGAT207.0299226E-445.031
TTAATCG207.0299226E-445.020
TTGTACG351.210301E-745.01
TCGATAG253.888016E-545.01
CCGCTAG207.0299226E-445.01
GTTACGC253.888016E-545.043
GCGATTC207.0299226E-445.09
TTACGAG453.8380676E-1045.01
ATCGGTT406.8030204E-945.028
GCGTATG207.0299226E-445.01
CCGCATA253.888016E-545.032
GCGTAAC207.0299226E-445.036
AATCGGT406.8030204E-945.027
CTTACGG253.888016E-545.02
TACGGCT6300.041.7857137
TACGAAT603.6379788E-1241.25000412
CGAATAT603.6379788E-1241.25000414
CGTTTTT10900.041.0779841
TTAACGG556.002665E-1140.9090922