##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536618
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.332884845458036	28.0	25.0	31.0	16.0	33.0
2	27.56062972170147	31.0	25.0	33.0	16.0	34.0
3	27.40586972483219	30.0	25.0	33.0	16.0	34.0
4	31.604411331710825	35.0	28.0	37.0	19.0	37.0
5	33.17817143666444	35.0	32.0	35.0	28.0	37.0
6	32.91105218237182	35.0	32.0	35.0	28.0	37.0
7	34.15276975427585	35.0	33.0	37.0	32.0	37.0
8	34.32034519900562	35.0	35.0	37.0	31.0	37.0
9	36.63710684322926	39.0	37.0	39.0	32.0	39.0
10	35.493224230271814	37.0	34.0	39.0	31.0	39.0
11	36.01831097726875	37.0	35.0	39.0	31.0	39.0
12	36.0115855226623	37.0	35.0	39.0	31.0	39.0
13	36.22960094517888	37.0	35.0	39.0	32.0	39.0
14	37.15237096034796	39.0	36.0	40.0	32.0	41.0
15	37.363252816715054	39.0	36.0	40.0	32.0	41.0
16	36.92004181745674	38.0	35.0	40.0	32.0	41.0
17	37.014731149532814	38.0	36.0	40.0	32.0	41.0
18	36.88712081965197	38.0	35.0	40.0	32.0	41.0
19	36.54094160091536	38.0	35.0	40.0	31.0	41.0
20	36.89095408651965	38.0	35.0	40.0	32.0	41.0
21	36.995134341375056	39.0	35.0	40.0	32.0	41.0
22	37.38661953195756	39.0	36.0	40.0	33.0	41.0
23	37.73231982527608	39.0	36.0	40.0	33.0	41.0
24	37.7894833941463	39.0	36.0	41.0	33.0	41.0
25	36.72190832212114	39.0	35.0	40.0	31.0	41.0
26	37.10345720792072	39.0	35.0	40.0	32.0	41.0
27	37.4136816133637	39.0	36.0	40.0	33.0	41.0
28	37.448736345035016	39.0	36.0	41.0	33.0	41.0
29	37.57245563883433	40.0	36.0	41.0	33.0	41.0
30	37.149025936513496	39.0	35.0	40.0	32.0	41.0
31	37.29543735021934	39.0	36.0	40.0	33.0	41.0
32	37.260088181909666	39.0	36.0	40.0	33.0	41.0
33	37.39719688866195	40.0	36.0	41.0	33.0	41.0
34	37.371491452019875	40.0	36.0	41.0	33.0	41.0
35	37.26028385182756	40.0	36.0	41.0	33.0	41.0
36	37.230292312222105	40.0	36.0	41.0	32.0	41.0
37	37.247302550417615	40.0	36.0	41.0	33.0	41.0
38	37.1213377113701	40.0	35.0	41.0	32.0	41.0
39	37.06350141068693	40.0	35.0	41.0	32.0	41.0
40	36.89362451501813	39.0	35.0	41.0	31.0	41.0
41	36.76269338710218	39.0	35.0	41.0	31.0	41.0
42	36.87363077645551	39.0	35.0	41.0	32.0	41.0
43	36.897489461777276	39.0	35.0	41.0	32.0	41.0
44	36.95951682574942	39.0	35.0	41.0	32.0	41.0
45	36.945765889329095	39.0	35.0	41.0	32.0	41.0
46	36.764834575060846	39.0	35.0	41.0	32.0	41.0
47	36.67554200567256	39.0	35.0	41.0	31.0	41.0
48	36.70210279938429	39.0	35.0	41.0	32.0	41.0
49	36.66152644898233	39.0	35.0	41.0	32.0	41.0
50	36.51120163691863	39.0	35.0	40.0	31.0	41.0
51	35.54884852912127	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	14.0
10	28.0
11	19.0
12	32.0
13	26.0
14	27.0
15	44.0
16	61.0
17	127.0
18	215.0
19	466.0
20	777.0
21	1187.0
22	1653.0
23	2150.0
24	2895.0
25	3805.0
26	4372.0
27	5134.0
28	5739.0
29	6771.0
30	8938.0
31	12242.0
32	18221.0
33	29083.0
34	39663.0
35	43710.0
36	66104.0
37	108169.0
38	132709.0
39	42234.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15751614742703	18.75505480621224	22.212635431536025	15.874793614824698
2	29.67119999701836	26.900513959651	26.084477225885077	17.34380881744556
3	31.319672467192678	24.82194037471721	26.770440052327725	17.087947105762385
4	27.79388689906041	25.023759918601314	28.76440969181056	18.417943490527712
5	27.7262410131602	29.228613277974276	24.293258891800125	18.7518868170654
6	25.179550443704834	35.49247323049171	24.043919510713394	15.284056815090064
7	77.74226731119717	5.952092550007641	11.690439008754831	4.615201130040364
8	79.0087175607229	7.704735957422226	8.63575206198823	4.650794419866646
9	73.12594806734026	7.714239924862752	9.922701064817058	9.237110942979923
10	40.223958197451445	26.99946703241412	18.403594363215547	14.37298040691889
11	30.39033353335147	24.690748353577405	26.081309236738235	18.837608876332883
12	26.660678546004796	22.32481951779478	29.76642602372638	21.24807591247405
13	25.106873045630223	23.824396498067525	31.65659743057445	19.412133025727798
14	20.879471057623856	28.09130517425804	29.649769482201492	21.37945428591661
15	20.015169077444288	26.69888076806965	33.737407243141305	19.548542911344754
16	23.341930386233784	25.772337118769777	29.518204756456175	21.367527738540264
17	23.134333920964263	27.029842457763248	29.065368660760544	20.770454960511948
18	23.111412587725347	27.148176170012935	28.825160542508826	20.915250699752896
19	23.910677614243276	28.844168477389875	26.534145332433873	20.711008575932972
20	24.765289274679567	27.834698053363844	29.496028832428284	17.903983839528305
21	24.34376036584684	28.59408368709212	28.132675385469742	18.9294805615913
22	22.408118997126447	25.086933349235395	30.02862371370323	22.476323939934925
23	22.253073881233952	27.519576309404453	29.434905277124507	20.792444532237084
24	23.370628640858115	25.63480911933629	29.347692399434983	21.646869840370616
25	21.123778926536197	30.03104629363905	27.305084808932982	21.54008997089177
26	21.394176117834288	28.122798713423702	28.897837940583432	21.585187228158578
27	22.442966877741707	28.639553648964437	27.843642963896105	21.073836509397747
28	20.19276282197019	28.685209963139513	30.215348721064146	20.90667849382615
29	23.654629550257354	26.183803003253715	28.174977358195214	21.98659008829372
30	22.409050758640227	29.68871711347737	27.62393359894748	20.278298528934922
31	23.95857015605142	28.339712793830994	25.685310593383004	22.016406456734586
32	24.638383356503134	29.01188554986974	25.819297899064136	20.530433194562985
33	24.74646769210127	27.687852438792586	25.239742237494827	22.325937631611314
34	24.78783790331297	27.22607143256469	27.06301316765371	20.923077496468622
35	22.24412897070169	29.155749527596914	26.235608943419713	22.36451255828168
36	23.088863959091942	31.411730504753848	25.295461576018695	20.203943960135515
37	21.732964604243616	30.256346227670335	27.365090250420227	20.645598917665826
38	22.46961525703573	29.926316299490512	25.217752665769687	22.386315777704066
39	24.471411693234295	27.37217163792493	26.241944921713394	21.91447174712738
40	24.347673764204707	27.20669079307813	27.314402424070757	21.131233018646412
41	21.938138489577316	29.703252593092294	26.00974995248016	22.34885896485023
42	21.645379021948575	29.34210183035232	27.243029492115433	21.769489655583673
43	23.10023144956002	27.85053799909806	26.21026503024498	22.838965521096945
44	22.30413441218893	26.795783965502462	27.263341893115772	23.636739729192836
45	22.8220074615462	26.49650216727728	26.418979609331032	24.262510761845483
46	24.147717743348153	28.528114971916708	25.655307872639384	21.668859412095756
47	21.151731771949507	27.24079326448237	29.743877395092973	21.863597568475154
48	22.664167061112376	26.980086392927554	27.46665225542192	22.889094290538146
49	22.43607184253976	26.021303795251	29.167303370367748	22.375320991841498
50	21.488097678423014	26.455877365276603	27.883336004383008	24.17268895191738
51	21.400512096127972	26.007886429452608	26.99145388339564	25.60014759102378
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	86.0
1	339.5
2	593.0
3	1798.5
4	3004.0
5	2129.5
6	1255.0
7	1274.5
8	1294.0
9	1263.0
10	1232.0
11	1207.0
12	1182.0
13	1210.5
14	1239.0
15	1309.5
16	1380.0
17	1324.5
18	1269.0
19	1402.5
20	1536.0
21	1669.5
22	1803.0
23	2012.0
24	2221.0
25	2503.0
26	3876.0
27	4967.0
28	5824.5
29	6682.0
30	7952.0
31	9222.0
32	11255.5
33	13289.0
34	14509.0
35	15729.0
36	17240.5
37	18752.0
38	20094.5
39	21437.0
40	23623.0
41	25809.0
42	28194.0
43	30579.0
44	33538.0
45	36497.0
46	47800.5
47	59104.0
48	53505.5
49	47907.0
50	47673.0
51	47439.0
52	43142.0
53	38845.0
54	35695.5
55	32546.0
56	29759.5
57	26973.0
58	24598.0
59	22223.0
60	20881.5
61	19540.0
62	16568.0
63	13596.0
64	11064.0
65	8532.0
66	7081.5
67	5631.0
68	4783.0
69	3935.0
70	3294.5
71	2654.0
72	2103.5
73	1553.0
74	1257.0
75	776.0
76	591.0
77	485.0
78	379.0
79	270.0
80	161.0
81	135.5
82	110.0
83	86.0
84	62.0
85	41.5
86	21.0
87	13.5
88	6.0
89	4.5
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	536618.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.01530794925662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76261475697099	25.090580561007492
2	9.301222518859111	6.327678965671062
3	3.419652790122157	3.4896162820661942
4	1.9903506691484656	2.708095637523762
5	1.3788386277465143	2.3450810267564055
6	1.0905224310363886	2.2256673790364836
7	0.8790382027081696	2.0930528614995616
8	0.7734314437778333	2.1046806990192928
9	0.6832028614267882	2.091542015092095
>10	6.572713744613412	43.37112894437416
>50	0.12248124050935053	2.5990188194077186
>100	0.019310105485708082	1.1419618395621156
>500	0.002758586497958298	0.7127643494916869
>1k	0.0038620210971416166	3.699130619492007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	4737	0.8827508581523542	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	4704	0.8766012321614258	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	4503	0.8391444193075893	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1891	0.35239220451047115	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1421	0.2648066222154307	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAACGGAAT	1280	0.23853094752691859	No Hit
GAACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCT	1175	0.21896395573760105	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCT	926	0.17256223235150517	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAACGGAATCGTA	893	0.16641260636057678	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	709	0.13212378265358224	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTC	705	0.13137837344256062	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	565	0.10528905105680392	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09578508361627824	0.0
2	0.0	0.0	0.0	0.7053434659292085	0.0
3	0.0	0.0	0.0	0.8946774055287001	0.0
4	0.0	0.0	0.0	1.4026737828399345	0.0
5	0.0	0.0	0.0	3.027479510564312	0.0
6	0.0	0.0	0.0	3.576100689876225	0.0
7	0.0	0.0	0.0	4.210630280758379	0.0
8	0.0	0.0	0.0	4.99088737239526	0.0
9	0.0	0.0	0.0	5.250103425528029	0.0
10	0.0	0.0	0.0	6.753407451855883	0.0
11	0.0	0.0	0.0	7.694113876165168	0.0
12	0.0	0.0	0.0	9.342399994036727	0.0
13	0.0	0.0	0.0	9.697401130785773	0.0
14	0.0	0.0	0.0	9.871454181559322	0.0
15	0.0	0.0	0.0	10.258694266685053	0.0
16	0.0	0.0	0.0	10.727556660417653	0.0
17	0.0	0.0	0.0	11.299658229876746	0.0
18	0.0	0.0	0.0	11.867473696372466	0.0
19	0.0	0.0	0.0	12.536664815567127	0.0
20	0.0	0.0	0.0	12.98465575139112	0.0
21	0.0	0.0	0.0	13.48184369514254	0.0
22	0.0	0.0	0.0	14.0820844623177	0.0
23	0.0	0.0	0.0	14.629960232418592	0.0
24	0.0	0.0	0.0	15.070683428435125	0.0
25	0.0	0.0	0.0	15.473390754689555	0.0
26	0.0	0.0	0.0	15.83211893749371	0.0
27	0.0	0.0	0.0	16.235571672959164	0.0
28	0.0	0.0	0.0	16.622439053479383	0.0
29	0.0	0.0	0.0	17.01843769683462	0.0
30	0.0	0.0	0.0	17.453011266860223	0.0
31	0.0	0.0	0.0	17.892989053665737	0.0
32	0.0	0.0	0.0	18.299423425975274	0.0
33	0.0	0.0	0.0	18.70026722920215	0.0
34	0.0	0.0	0.0	19.091979769594012	0.0
35	1.8635230275540514E-4	0.0	0.0	19.496923323481507	0.0
36	1.8635230275540514E-4	0.0	0.0	19.912302606323305	0.0
37	1.8635230275540514E-4	0.0	0.0	20.32432754771551	0.0
38	1.8635230275540514E-4	0.0	0.0	20.765609800640306	0.0
39	1.8635230275540514E-4	0.0	0.0	21.210246395014703	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	20	7.0299226E-4	45.0	1
ACACGAT	20	7.0299226E-4	45.0	31
TTAATCG	20	7.0299226E-4	45.0	20
TTGTACG	35	1.210301E-7	45.0	1
TCGATAG	25	3.888016E-5	45.0	1
CCGCTAG	20	7.0299226E-4	45.0	1
GTTACGC	25	3.888016E-5	45.0	43
GCGATTC	20	7.0299226E-4	45.0	9
TTACGAG	45	3.8380676E-10	45.0	1
ATCGGTT	40	6.8030204E-9	45.0	28
GCGTATG	20	7.0299226E-4	45.0	1
CCGCATA	25	3.888016E-5	45.0	32
GCGTAAC	20	7.0299226E-4	45.0	36
AATCGGT	40	6.8030204E-9	45.0	27
CTTACGG	25	3.888016E-5	45.0	2
TACGGCT	630	0.0	41.785713	7
TACGAAT	60	3.6379788E-12	41.250004	12
CGAATAT	60	3.6379788E-12	41.250004	14
CGTTTTT	1090	0.0	41.077984	1
TTAACGG	55	6.002665E-11	40.909092	2
>>END_MODULE