FastQCFastQC Report
Sat 18 Jun 2016
SRR3551817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687507
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC68510.9964989447380171No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG64370.9362813760441713No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC56320.8191916591394707No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26900.3912687434455213No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC18530.2695245284775282No Hit
GAACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT15100.21963412736161234No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACATCAT14380.2091615067192043No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT13450.19563437172276066No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC11360.16523468124688184No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACATCATCGTA10620.15447115447551807No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC7260.10559892481094738No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACC253.889163E-545.00000433
TAACGCG253.889163E-545.0000041
ACGTAAG253.889163E-545.0000041
CGCGTTA253.889163E-545.00000415
CCATTCG253.889163E-545.00000410
TTGTGCG406.8084773E-945.01
AAGACCG207.031307E-445.036
TCGATAG207.031307E-445.01
AACGCGT207.031307E-445.044
TTGCGAG406.8084773E-945.01
CATACGT207.031307E-445.031
CTCGCGT207.031307E-445.011
TCGTAGG207.031307E-445.02
TTACGGA207.031307E-445.026
ATCGGTA207.031307E-445.024
CTAGGCG207.031307E-445.01
TTTAGCG302.1642245E-644.9999961
CGTTTTT18000.042.1251
GCTACGA1000.040.50000410
ACGTTAG451.9263098E-840.0000041