##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	687507
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.527476811145196	28.0	25.0	31.0	16.0	33.0
2	27.57419488092485	31.0	25.0	33.0	16.0	34.0
3	27.474124627094707	31.0	25.0	33.0	16.0	34.0
4	31.681867966435252	35.0	28.0	37.0	19.0	37.0
5	33.19745689862067	35.0	32.0	35.0	28.0	37.0
6	32.97486280139693	35.0	32.0	35.0	28.0	37.0
7	34.10054733988163	35.0	33.0	37.0	31.0	37.0
8	34.15512860232696	35.0	35.0	37.0	31.0	37.0
9	36.543382103745856	39.0	37.0	39.0	32.0	39.0
10	35.53738652842807	37.0	34.0	39.0	31.0	39.0
11	36.06532297125702	37.0	35.0	39.0	31.0	39.0
12	36.06809821572726	37.0	35.0	39.0	32.0	39.0
13	36.22156574405788	37.0	35.0	39.0	32.0	39.0
14	37.15774966654885	39.0	36.0	40.0	32.0	41.0
15	37.34983207443706	39.0	36.0	40.0	32.0	41.0
16	36.99770475064254	38.0	35.0	40.0	32.0	41.0
17	37.05757468651228	38.0	36.0	40.0	32.0	41.0
18	36.86934823936338	38.0	35.0	40.0	32.0	41.0
19	36.5566299688585	38.0	35.0	40.0	31.0	41.0
20	36.85725818064398	38.0	35.0	40.0	32.0	41.0
21	36.97211955660088	39.0	35.0	40.0	32.0	41.0
22	37.350569521473965	39.0	36.0	40.0	32.0	41.0
23	37.69434347577552	39.0	36.0	40.0	33.0	41.0
24	37.746900031563314	40.0	36.0	41.0	33.0	41.0
25	36.764219127950696	39.0	35.0	40.0	31.0	41.0
26	37.0952484847427	39.0	35.0	40.0	32.0	41.0
27	37.41015727839862	39.0	36.0	40.0	33.0	41.0
28	37.428138186229376	39.0	36.0	41.0	33.0	41.0
29	37.5704611007597	40.0	36.0	41.0	33.0	41.0
30	37.17208842964508	39.0	35.0	41.0	32.0	41.0
31	37.29420936805007	39.0	36.0	40.0	33.0	41.0
32	37.21528362620308	39.0	36.0	40.0	33.0	41.0
33	37.295743897880314	40.0	36.0	41.0	33.0	41.0
34	37.32464833085336	40.0	36.0	41.0	33.0	41.0
35	37.22411553627818	40.0	36.0	41.0	33.0	41.0
36	37.17505858122172	40.0	36.0	41.0	32.0	41.0
37	37.220661025996826	40.0	36.0	41.0	33.0	41.0
38	37.041000309815026	40.0	35.0	41.0	32.0	41.0
39	37.01809436122105	40.0	35.0	41.0	32.0	41.0
40	36.88510371530763	39.0	35.0	41.0	31.0	41.0
41	36.71841304888532	39.0	35.0	41.0	31.0	41.0
42	36.889796031167684	39.0	35.0	41.0	32.0	41.0
43	36.90128391420015	40.0	35.0	41.0	32.0	41.0
44	36.953543745736404	40.0	35.0	41.0	32.0	41.0
45	36.94206168082652	39.0	35.0	41.0	32.0	41.0
46	36.765891838192196	39.0	35.0	41.0	32.0	41.0
47	36.723636268430724	39.0	35.0	41.0	31.0	41.0
48	36.761333339151456	39.0	35.0	41.0	32.0	41.0
49	36.72557515778021	39.0	35.0	41.0	32.0	41.0
50	36.63785677818553	39.0	35.0	41.0	32.0	41.0
51	35.756645386883335	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	30.0
10	33.0
11	37.0
12	31.0
13	22.0
14	51.0
15	68.0
16	80.0
17	155.0
18	256.0
19	503.0
20	944.0
21	1670.0
22	2403.0
23	3077.0
24	4180.0
25	5371.0
26	6248.0
27	6746.0
28	7494.0
29	8712.0
30	11167.0
31	15326.0
32	22390.0
33	36170.0
34	51095.0
35	53139.0
36	81092.0
37	135355.0
38	175222.0
39	58434.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63507717012336	18.927952733572166	21.978539854866934	18.458430241437544
2	29.749515277662624	27.596519017260917	24.532986573227618	18.120979131848838
3	31.115901365367915	25.071017458731333	26.945325647593403	16.86775552830735
4	28.573672704423377	24.308697947802713	28.618617701347045	18.499011646426872
5	28.39912902704991	27.396230147474864	24.431460334222052	19.773180491253182
6	25.417777564446613	35.29447700168871	24.747238937203548	14.540506496661123
7	76.28547782058946	6.083138062594272	12.963795277720807	4.6675888390954565
8	77.46510217350514	8.692275133198644	9.004562862632671	4.838059830663542
9	71.15825729774387	8.635839344181223	10.528474619167513	9.677428738907386
10	39.98388380045585	28.63098121182766	17.80665505951212	13.578479928204368
11	30.114311563373175	24.666221580289363	27.36932133054645	17.85014552579101
12	27.571791996299673	21.923122237300856	29.649007210108408	20.856078556291063
13	25.09836263485317	23.929501808708856	32.163454335737676	18.8086812207003
14	19.8421252438157	28.759561720826117	29.085231132192106	22.313081903166076
15	19.01173369871143	26.7059099034628	34.21448799794039	20.067868399885384
16	22.87016714011639	26.463294191913683	29.746606216373067	20.919932451596857
17	22.475698429252354	26.846126657619486	29.261956605532745	21.416218307595415
18	21.23338380554671	27.180959612047584	29.648570850914975	21.937085731490736
19	23.410961633845183	28.19636745516773	27.57979191484596	20.81287899614113
20	25.701411040178503	27.68655446417273	29.553008187552997	17.059026308095774
21	24.351024789565777	28.958396059967388	28.44276494639327	18.24781420407356
22	21.86581372989657	24.794365730094384	30.044203186294833	23.295617353714217
23	22.344063405899867	26.97237991758629	30.57205235728509	20.11150431922875
24	22.645878514691486	26.050643847989914	29.470681753058514	21.832795884260086
25	21.00924063318628	29.33759219906125	28.080150456649893	21.57301671110258
26	20.607062909904915	28.403492618984245	28.6267630729578	22.362681398153036
27	22.178101459330595	28.192003863233396	28.746034585829673	20.88386009160634
28	18.94424347679369	28.75170725534431	31.167537203257567	21.13651206460443
29	21.108875982353634	26.222569370202777	29.332646794869	23.335907852574593
30	20.775497558570315	28.315929874168553	30.371181675241125	20.537390892020007
31	22.286900351560057	27.82895301429658	26.78460001134534	23.099546622798023
32	23.184636665517587	28.597817913126704	26.18431521424509	22.03323020711062
33	22.235264513670405	29.106612732670357	26.616892628002333	22.041230125656902
34	20.32139309127034	27.43157524214299	28.362474854801476	23.884556811785192
35	20.331574805783795	26.928307639049493	28.149095209212415	24.591022345954297
36	20.070341101981505	31.365789730140932	26.345477209686592	22.21839195819097
37	20.189030802595465	29.06035865816639	29.313592443422394	21.437018095815752
38	20.435573746885485	30.310236841224892	26.164242691347145	23.089946720542482
39	21.485017607093454	27.36204867732256	28.346620470773388	22.806313244810596
40	21.420290993400794	26.741982263453316	29.17832109345796	22.65940564968793
41	19.239513197683806	28.0676414931048	28.760288986148502	23.932556323062894
42	19.250858536713082	27.592446331455534	29.288719969396677	23.86797516243471
43	21.527489901920998	27.2256137028423	27.351721509744625	23.895174885492075
44	20.631353571672726	27.040451951761945	28.419056096883377	23.909138379681952
45	21.313528444074024	26.217769419075005	27.28001314895703	25.188688987893944
46	21.773887393146545	28.791706848075727	26.680019257985737	22.75438650079199
47	19.28940359879972	27.613973384998264	30.547470789388324	22.54915222681369
48	20.33593839771813	26.949543786463266	29.100794610091242	23.61372320572736
49	20.099868074070518	26.08249806911057	31.13219210858944	22.685441748229472
50	20.395574154154065	27.007434106125466	28.778034260014806	23.81895747970566
51	19.750635266259106	25.47683150862464	27.958697147810856	26.813836077305396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	183.0
1	553.0
2	923.0
3	3059.5
4	5196.0
5	3680.5
6	2165.0
7	2103.5
8	2042.0
9	1998.0
10	1954.0
11	1947.5
12	1941.0
13	2014.0
14	2087.0
15	2017.5
16	1948.0
17	1859.0
18	1770.0
19	1854.0
20	1938.0
21	1976.5
22	2015.0
23	2416.5
24	2818.0
25	3747.0
26	6478.5
27	8281.0
28	9573.0
29	10865.0
30	12627.0
31	14389.0
32	15720.0
33	17051.0
34	19698.0
35	22345.0
36	23725.0
37	25105.0
38	26735.0
39	28365.0
40	31676.0
41	34987.0
42	37919.5
43	40852.0
44	57647.5
45	74443.0
46	67901.5
47	61360.0
48	58175.0
49	54990.0
50	50893.5
51	46797.0
52	43611.0
53	40425.0
54	37577.0
55	34729.0
56	32490.0
57	30251.0
58	29529.5
59	28808.0
60	26611.0
61	24414.0
62	22329.5
63	20245.0
64	17192.5
65	14140.0
66	11279.0
67	8418.0
68	6808.5
69	5199.0
70	4402.0
71	3605.0
72	2927.5
73	2250.0
74	1863.0
75	1154.0
76	832.0
77	696.5
78	561.0
79	440.0
80	319.0
81	251.5
82	184.0
83	157.0
84	130.0
85	76.5
86	23.0
87	14.0
88	5.0
89	5.0
90	5.0
91	3.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	687507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.737656978019654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52818808561035	20.394996593339712
2	9.474596928500581	5.256062392154954
3	3.393863909573791	2.8241349886151554
4	1.725031201719223	1.9139329499867532
5	1.1125399803300022	1.542962617436317
6	0.848854606542431	1.4127142740291472
7	0.6674847971625106	1.296012503921572
8	0.5796033965459557	1.2861472157349456
9	0.5449116610544141	1.3603115463885223
>10	7.629733437262791	48.1515204916678
>50	0.4450392882445302	7.551431038947068
>100	0.042233858908131076	2.157405291876632
>500	0.0026396161817581923	0.4660979463818177
>1k	0.003695462654461469	1.6157476407090718
>5k	0.0015837697090549155	2.7705225088105525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	6851	0.9964989447380171	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	6437	0.9362813760441713	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC	5632	0.8191916591394707	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2690	0.3912687434455213	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1853	0.2695245284775282	No Hit
GAACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	1510	0.21963412736161234	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTACATCAT	1438	0.2091615067192043	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT	1345	0.19563437172276066	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC	1136	0.16523468124688184	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTACATCATCGTA	1062	0.15447115447551807	No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	726	0.10559892481094738	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4545306447788896E-4	0.0	0.0	0.0644357075637048	0.0
2	1.4545306447788896E-4	0.0	0.0	0.5405035875998353	0.0
3	1.4545306447788896E-4	0.0	0.0	0.7124291098127001	0.0
4	1.4545306447788896E-4	0.0	0.0	1.1745334956589533	0.0
5	1.4545306447788896E-4	0.0	0.0	2.802007834102053	0.0
6	1.4545306447788896E-4	0.0	0.0	3.30862085767854	0.0
7	1.4545306447788896E-4	0.0	0.0	3.895378519782344	0.0
8	1.4545306447788896E-4	0.0	0.0	4.60606219282131	0.0
9	1.4545306447788896E-4	0.0	0.0	4.825405414053966	0.0
10	1.4545306447788896E-4	0.0	0.0	6.270336156577315	0.0
11	1.4545306447788896E-4	0.0	0.0	7.091709611683954	0.0
12	1.4545306447788896E-4	0.0	0.0	8.551622019848525	0.0
13	1.4545306447788896E-4	0.0	0.0	8.857655267510003	0.0
14	1.4545306447788896E-4	0.0	0.0	8.985799417315024	0.0
15	1.4545306447788896E-4	0.0	0.0	9.311614281745495	0.0
16	1.4545306447788896E-4	0.0	0.0	9.711755662124167	0.0
17	1.4545306447788896E-4	0.0	0.0	10.167314660068916	0.0
18	1.4545306447788896E-4	0.0	0.0	10.657055128165968	0.0
19	1.4545306447788896E-4	0.0	0.0	11.216613067212407	0.0
20	1.4545306447788896E-4	0.0	0.0	11.571809450667411	0.0
21	1.4545306447788896E-4	0.0	0.0	11.988968839589997	0.0
22	1.4545306447788896E-4	0.0	0.0	12.453400474467896	0.0
23	1.4545306447788896E-4	0.0	0.0	12.90110500693084	0.0
24	1.4545306447788896E-4	0.0	0.0	13.24815601877508	0.0
25	1.4545306447788896E-4	0.0	0.0	13.570480009658084	0.0
26	1.4545306447788896E-4	0.0	0.0	13.854404391518923	0.0
27	1.4545306447788896E-4	0.0	0.0	14.178328366111181	0.0
28	1.4545306447788896E-4	0.0	0.0	14.494106969092678	0.0
29	1.4545306447788896E-4	0.0	0.0	14.827630845940478	0.0
30	1.4545306447788896E-4	0.0	0.0	15.208426968743591	0.0
31	1.4545306447788896E-4	0.0	0.0	15.561005197037995	0.0
32	1.4545306447788896E-4	0.0	0.0	15.911401629365228	0.0
33	1.4545306447788896E-4	0.0	0.0	16.25583448604887	0.0
34	1.4545306447788896E-4	0.0	0.0	16.58644930160711	0.0
35	1.4545306447788896E-4	0.0	0.0	16.951245587317658	0.0
36	1.4545306447788896E-4	0.0	0.0	17.30731468915953	0.0
37	1.4545306447788896E-4	0.0	0.0	17.665420133904092	0.0
38	1.4545306447788896E-4	0.0	0.0	18.02992551348568	0.0
39	1.4545306447788896E-4	0.0	0.0	18.399230844195042	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAACC	25	3.889163E-5	45.000004	33
TAACGCG	25	3.889163E-5	45.000004	1
ACGTAAG	25	3.889163E-5	45.000004	1
CGCGTTA	25	3.889163E-5	45.000004	15
CCATTCG	25	3.889163E-5	45.000004	10
TTGTGCG	40	6.8084773E-9	45.0	1
AAGACCG	20	7.031307E-4	45.0	36
TCGATAG	20	7.031307E-4	45.0	1
AACGCGT	20	7.031307E-4	45.0	44
TTGCGAG	40	6.8084773E-9	45.0	1
CATACGT	20	7.031307E-4	45.0	31
CTCGCGT	20	7.031307E-4	45.0	11
TCGTAGG	20	7.031307E-4	45.0	2
TTACGGA	20	7.031307E-4	45.0	26
ATCGGTA	20	7.031307E-4	45.0	24
CTAGGCG	20	7.031307E-4	45.0	1
TTTAGCG	30	2.1642245E-6	44.999996	1
CGTTTTT	1800	0.0	42.125	1
GCTACGA	100	0.0	40.500004	10
ACGTTAG	45	1.9263098E-8	40.000004	1
>>END_MODULE