FastQCFastQC Report
Sat 18 Jun 2016
SRR3551812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385350
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG63301.6426625145971194No Hit
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC62631.6252757233683666No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC59061.5326326715972494No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC24530.636564162449721TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.48812767613857533No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT18440.47852601531075645No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT13720.35603996366939145No Hit
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT11470.29765148566238486No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC11370.2950564421954068TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA9350.24263656416244972No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC8420.21850265991955364No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT7130.18502659919553655TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC4450.1154794342805242No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAG453.8380676E-1045.0000041
TGATCCG207.027445E-445.04
TCGCACA207.027445E-445.034
AGATCCG253.885963E-545.016
CGAACCC207.027445E-445.034
GGTAATC253.885963E-545.08
CGTATGG253.885963E-545.02
CGTGCTT253.885963E-545.040
GTTAGCG207.027445E-445.01
CACGAAG253.885963E-545.01
GCGCAAG253.885963E-545.01
CCGTATG207.027445E-445.01
AGTGCGA207.027445E-445.010
CTAACGG207.027445E-445.02
CTACGAA551.8189894E-1245.011
TACGAAT551.8189894E-1245.012
ATCCACC207.027445E-445.012
TATAGCG253.885963E-545.01
ATGCGAG207.027445E-445.01
TGTAGCG253.885963E-545.01
Produced by FastQC (version 0.11.5)