##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551812_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 385350 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.47260672116258 28.0 25.0 31.0 16.0 33.0 2 27.532339431685482 30.0 25.0 33.0 16.0 34.0 3 27.54728947709874 30.0 25.0 33.0 16.0 34.0 4 31.74 35.0 28.0 35.0 19.0 37.0 5 33.25178149734008 35.0 32.0 35.0 28.0 37.0 6 33.071810042818214 35.0 32.0 35.0 28.0 37.0 7 34.02069547164915 35.0 33.0 37.0 31.0 37.0 8 33.953437135072015 35.0 35.0 37.0 30.0 37.0 9 36.11813416374724 39.0 35.0 39.0 31.0 39.0 10 35.383347606072405 37.0 34.0 39.0 31.0 39.0 11 35.96867782535357 37.0 35.0 39.0 31.0 39.0 12 35.95131698455949 37.0 35.0 39.0 31.0 39.0 13 35.94753081614117 37.0 35.0 39.0 31.0 39.0 14 36.86438043337226 39.0 36.0 40.0 31.0 41.0 15 37.07777345270533 39.0 36.0 40.0 32.0 41.0 16 36.68223692746854 38.0 35.0 40.0 32.0 41.0 17 36.79863760217984 38.0 35.0 40.0 32.0 41.0 18 36.68752303101077 38.0 35.0 40.0 32.0 41.0 19 36.26759309718438 38.0 34.0 40.0 30.0 41.0 20 36.56481899571818 38.0 35.0 40.0 31.0 41.0 21 36.81967561956663 38.0 35.0 40.0 32.0 41.0 22 37.14117814973401 39.0 35.0 40.0 32.0 41.0 23 37.47873361878811 39.0 35.0 40.0 33.0 41.0 24 37.52569612041002 39.0 35.0 41.0 33.0 41.0 25 36.6119605553393 38.0 35.0 40.0 31.0 41.0 26 36.85852861035422 39.0 35.0 40.0 32.0 41.0 27 37.10595821980018 39.0 35.0 40.0 32.0 41.0 28 37.11712988192552 39.0 35.0 40.0 32.0 41.0 29 37.19515245880368 39.0 35.0 41.0 32.0 41.0 30 36.83229272090308 39.0 35.0 40.0 31.0 41.0 31 36.87587647593097 39.0 35.0 40.0 31.0 41.0 32 36.68790709744388 39.0 35.0 40.0 31.0 41.0 33 36.78905410665629 39.0 35.0 40.0 31.0 41.0 34 36.81444660698067 39.0 35.0 41.0 31.0 41.0 35 36.71360581289736 39.0 35.0 41.0 31.0 41.0 36 36.74009601660828 39.0 35.0 41.0 31.0 41.0 37 36.708093940573505 39.0 35.0 41.0 31.0 41.0 38 36.505421045802514 39.0 35.0 41.0 31.0 41.0 39 36.526648501362395 39.0 35.0 40.0 31.0 41.0 40 36.33371999480991 39.0 35.0 40.0 30.0 41.0 41 36.27899052809135 39.0 35.0 40.0 30.0 41.0 42 36.426456468145844 39.0 35.0 40.0 31.0 41.0 43 36.430868042039705 39.0 35.0 40.0 31.0 41.0 44 36.5033320358116 39.0 35.0 40.0 31.0 41.0 45 36.50546516154146 39.0 35.0 40.0 31.0 41.0 46 36.358603866614764 39.0 35.0 40.0 31.0 41.0 47 36.20141948877644 39.0 35.0 40.0 30.0 41.0 48 36.27366290385364 39.0 35.0 40.0 31.0 41.0 49 36.315559880628 39.0 35.0 40.0 31.0 41.0 50 36.20831711431166 39.0 35.0 40.0 31.0 41.0 51 35.319047619047616 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 14.0 10 16.0 11 19.0 12 16.0 13 21.0 14 18.0 15 25.0 16 36.0 17 72.0 18 149.0 19 288.0 20 551.0 21 995.0 22 1539.0 23 1975.0 24 2619.0 25 3221.0 26 4035.0 27 4413.0 28 4766.0 29 5574.0 30 7102.0 31 10001.0 32 14539.0 33 23520.0 34 32504.0 35 32766.0 36 47006.0 37 74963.0 38 87421.0 39 25165.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.93213961333852 17.29336966394187 18.815622161671207 18.958868561048398 2 26.692876605683146 30.393149085247178 23.817308939924743 19.096665369144933 3 30.727131179447255 27.28506552484754 25.541974828078366 16.445828467626832 4 28.17231088620734 22.535616971584275 31.018035552095498 18.274036590112885 5 29.900869339561435 26.49124172829895 23.855456078889322 19.752432853250294 6 24.097314130011675 36.37472427663163 24.119112495134292 15.408849098222396 7 71.52147398468924 6.553263267159724 16.560788893213964 5.36447385493707 8 70.85506682236927 11.30764240301025 11.545348384585443 6.291942390035032 9 65.68677825353575 7.689632801349422 12.458803684961724 14.164785260153106 10 41.42078629817049 23.277539898793304 20.478526015310756 14.823147787725443 11 33.19891008174387 23.624237705981574 24.480861554431037 18.69599065784352 12 29.659789801479175 20.441676398079668 29.14000259504347 20.75853120539769 13 25.230569612041005 23.78201634877384 31.995847930452836 18.99156610873232 14 19.66368236667964 30.313481250810952 27.835733748540285 22.18710263396912 15 17.62968729726223 24.720384066433112 37.48436486311146 20.165563773193202 16 21.07330997794213 24.013494226028286 30.699883223043983 24.213312572985597 17 20.328013494226028 26.298949007395873 29.555728558453353 23.817308939924743 18 20.188919164396005 26.688984040482676 30.352147398468926 22.769949396652393 19 22.261061372777995 27.279356429220194 27.37874659400545 23.080835603996366 20 25.449331776307254 26.723757622940187 30.451537563254185 17.375373037498377 21 23.83858829635396 28.52679382379655 28.604126119112493 19.030491760736993 22 20.59089139743091 24.713896457765667 29.842740365901125 24.852471778902295 23 21.962371869728816 27.361878811470092 29.408330089529 21.267419229272093 24 22.95030491760737 24.805501492149993 28.842091605034387 23.40210198520825 25 20.273777085766188 30.711560918645386 27.17633320358116 21.838328792007268 26 19.177890229661347 27.857272609316208 29.816789931231348 23.1480472297911 27 22.46970286752303 27.975087582717013 27.31724406383807 22.23796548592189 28 17.63435837550279 29.485921889191648 31.50512521084728 21.374594524458285 29 20.652393927598286 27.409108602569095 28.67808485792137 23.26041261191125 30 18.956792526274814 28.86233294407681 28.160373686259245 24.020500843389126 31 21.618269106007524 29.114571169067084 24.301284546516154 24.965875178409238 32 23.484105358764758 31.027637212923313 24.011158686908004 21.477098741403918 33 20.97288179577008 26.448942519787206 26.169715842740366 26.408459841702346 34 20.40716231996886 28.940962761126247 25.534708706370836 25.11716621253406 35 19.550019462826 26.030102504216945 25.570260801868432 28.84961723108862 36 20.175943947061114 31.01985208252238 24.089009990917347 24.715193979499155 37 19.035422343324253 29.614117036460364 27.784092383547428 23.566368236667966 38 19.87907097443882 29.49837809783314 25.3019333073829 25.320617620345143 39 21.56455170624108 28.133385234202674 26.501362397820166 23.800700661736084 40 20.262099390164785 24.890618917866874 29.537563254184505 25.309718437783836 41 18.313481250810952 26.41884001557026 24.910341248215907 30.35733748540288 42 19.392500324380432 24.61684183210069 28.97417931750357 27.01647852601531 43 22.17983651226158 25.142857142857146 25.537044245491114 27.140262099390167 44 20.324639937718956 25.497340080446346 28.05579343454003 26.12222654729467 45 20.039444660698067 25.549500454132605 25.993252886985857 28.417801998183467 46 23.040871934604905 28.534059945504087 24.821071752951863 23.603996366939146 47 17.726741922927207 25.784351887894125 32.21980018165304 24.269106007525625 48 20.11989100817439 25.426754898144544 27.49240949785909 26.960944595821978 49 19.660827818865968 22.987154534838456 32.735694822888284 24.61632282340729 50 19.810042818217205 24.405086285195278 29.05125210847282 26.7336187881147 51 19.219410925132998 24.07473725184897 26.620734397301156 30.085117425716877 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148.0 1 310.0 2 472.0 3 1951.0 4 3430.0 5 2392.5 6 1355.0 7 1354.0 8 1353.0 9 1317.5 10 1282.0 11 1246.5 12 1211.0 13 1210.0 14 1209.0 15 1125.5 16 1042.0 17 1039.0 18 1036.0 19 932.5 20 829.0 21 993.5 22 1158.0 23 1287.0 24 1416.0 25 1880.0 26 2689.0 27 3034.0 28 4085.5 29 5137.0 30 5564.5 31 5992.0 32 6401.0 33 6810.0 34 7524.0 35 8238.0 36 9755.0 37 11272.0 38 11940.0 39 12608.0 40 14847.5 41 17087.0 42 18680.5 43 20274.0 44 22432.5 45 24591.0 46 38733.0 47 52875.0 48 44437.5 49 36000.0 50 35615.5 51 35231.0 52 30341.0 53 25451.0 54 22448.5 55 19446.0 56 18571.0 57 17696.0 58 16617.0 59 15538.0 60 14896.5 61 14255.0 62 13128.5 63 12002.0 64 10583.5 65 9165.0 66 7134.5 67 5104.0 68 4089.5 69 3075.0 70 2622.5 71 2170.0 72 1901.5 73 1633.0 74 1253.0 75 778.0 76 683.0 77 538.5 78 394.0 79 311.0 80 228.0 81 173.0 82 118.0 83 90.0 84 62.0 85 36.5 86 11.0 87 7.5 88 4.0 89 4.0 90 4.0 91 3.5 92 3.0 93 1.5 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 385350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.112061548185252 #Duplication Level Percentage of deduplicated Percentage of total 1 69.7593932851435 18.913209643108715 2 12.186230830714104 6.607876806454269 3 4.454888349872695 3.623436213961258 4 2.0999155924474313 2.2773216315371463 5 1.3103212684740997 1.776275543938298 6 0.884740501574632 1.4392283359718228 7 0.7014025318642234 1.3311528029769055 8 0.556935733909309 1.2079740716906329 9 0.48021981847297424 1.1717774347587864 >10 7.11661782531448 44.5575209016673 >50 0.38955586812664045 6.346181658362081 >100 0.04782271526874358 2.4221593143156883 >500 0.0029889197042964736 0.6725969323793981 >1k 0.005977839408592947 2.6563527040236954 >5k 0.0029889197042964736 4.99693600485401 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG 6330 1.6426625145971194 No Hit GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC 6263 1.6252757233683666 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC 5906 1.5326326715972494 No Hit GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC 2453 0.636564162449721 TruSeq Adapter, Index 19 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1881 0.48812767613857533 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT 1844 0.47852601531075645 No Hit GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT 1372 0.35603996366939145 No Hit GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT 1147 0.29765148566238486 No Hit CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC 1137 0.2950564421954068 TruSeq Adapter, Index 19 (95% over 21bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA 935 0.24263656416244972 No Hit GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC 842 0.21850265991955364 No Hit CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT 713 0.18502659919553655 TruSeq Adapter, Index 13 (95% over 22bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC 445 0.1154794342805242 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1915142078629817 0.0 2 0.0 0.0 0.0 1.33255482029324 0.0 3 0.0 0.0 0.0 1.609964966913196 0.0 4 0.0 0.0 0.0 2.29349941611522 0.0 5 0.0 0.0 0.0 5.002465291293629 0.0 6 0.0 0.0 0.0 5.613079019073569 0.0 7 0.0 0.0 0.0 6.620993901647853 0.0 8 0.0 0.0 0.0 7.615674062540547 0.0 9 0.0 0.0 0.0 7.914363565589723 0.0 10 0.0 0.0 0.0 10.321785389905282 0.0 11 0.0 0.0 0.0 11.506941741274167 0.0 12 0.0 0.0 0.0 14.083041390943299 0.0 13 0.0 0.0 0.0 14.622550927728039 0.0 14 0.0 0.0 0.0 14.834046970286753 0.0 15 0.0 0.0 0.0 15.429609445958219 0.0 16 0.0 0.0 0.0 16.103542234332426 0.0 17 0.0 0.0 0.0 16.8047229791099 0.0 18 0.0 0.0 0.0 17.546126897625534 0.0 19 0.0 0.0 0.0 18.530426884650318 0.0 20 0.0 0.0 0.0 19.086025690930324 0.0 21 0.0 0.0 0.0 19.67068898404048 0.0 22 0.0 0.0 0.0 20.328532502919423 0.0 23 0.0 0.0 0.0 20.938367717659272 0.0 24 0.0 0.0 0.0 21.44492020241339 0.0 25 0.0 0.0 0.0 21.8461139224082 0.0 26 0.0 0.0 0.0 22.248345659789802 0.0 27 0.0 0.0 0.0 22.71104190995199 0.0 28 0.0 0.0 0.0 23.104969508239265 0.0 29 0.0 0.0 0.0 23.495523550019463 0.0 30 0.0 0.0 0.0 23.968599974049564 0.0 31 0.0 0.0 0.0 24.417542493836773 0.0 32 0.0 0.0 0.0 24.884650317892824 0.0 33 0.0 0.0 0.0 25.29336966394187 0.0 34 0.0 0.0 0.0 25.69118982742961 0.0 35 0.0 0.0 0.0 26.140132347216817 0.0 36 0.0 0.0 0.0 26.589853380044115 0.0 37 2.595043466978072E-4 0.0 0.0 27.024523160762943 0.0 38 2.595043466978072E-4 0.0 0.0 27.419488776437007 0.0 39 2.595043466978072E-4 0.0 0.0 27.840404826780848 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTAAG 45 3.8380676E-10 45.000004 1 TGATCCG 20 7.027445E-4 45.0 4 TCGCACA 20 7.027445E-4 45.0 34 AGATCCG 25 3.885963E-5 45.0 16 CGAACCC 20 7.027445E-4 45.0 34 GGTAATC 25 3.885963E-5 45.0 8 CGTATGG 25 3.885963E-5 45.0 2 CGTGCTT 25 3.885963E-5 45.0 40 GTTAGCG 20 7.027445E-4 45.0 1 CACGAAG 25 3.885963E-5 45.0 1 GCGCAAG 25 3.885963E-5 45.0 1 CCGTATG 20 7.027445E-4 45.0 1 AGTGCGA 20 7.027445E-4 45.0 10 CTAACGG 20 7.027445E-4 45.0 2 CTACGAA 55 1.8189894E-12 45.0 11 TACGAAT 55 1.8189894E-12 45.0 12 ATCCACC 20 7.027445E-4 45.0 12 TATAGCG 25 3.885963E-5 45.0 1 ATGCGAG 20 7.027445E-4 45.0 1 TGTAGCG 25 3.885963E-5 45.0 1 >>END_MODULE