##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551811_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	352021
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.514287499893474	28.0	25.0	31.0	16.0	33.0
2	27.652265063731992	31.0	25.0	33.0	16.0	34.0
3	27.721087662383777	31.0	25.0	33.0	16.0	34.0
4	31.76682072944512	35.0	28.0	37.0	19.0	37.0
5	33.32113709125308	35.0	32.0	35.0	28.0	37.0
6	33.06932825030325	35.0	32.0	35.0	28.0	37.0
7	33.9989943781763	35.0	33.0	37.0	31.0	37.0
8	33.97911772309038	35.0	35.0	37.0	30.0	37.0
9	36.127535005013904	39.0	35.0	39.0	31.0	39.0
10	35.449268651586124	37.0	34.0	39.0	31.0	39.0
11	35.993591291428636	37.0	35.0	39.0	31.0	39.0
12	35.90770721064936	37.0	35.0	39.0	31.0	39.0
13	35.96335161822732	37.0	35.0	39.0	31.0	39.0
14	36.855554072058204	39.0	36.0	40.0	31.0	41.0
15	37.07577956996884	39.0	36.0	40.0	32.0	41.0
16	36.568500174705484	38.0	34.0	40.0	31.0	41.0
17	36.746495237500035	38.0	35.0	40.0	32.0	41.0
18	36.591586865556316	38.0	35.0	40.0	31.0	41.0
19	36.1402700407078	38.0	34.0	40.0	30.0	41.0
20	36.47764479960002	38.0	35.0	40.0	31.0	41.0
21	36.75454589356885	38.0	35.0	40.0	32.0	41.0
22	37.12050133372725	39.0	35.0	40.0	32.0	41.0
23	37.449129455344995	39.0	35.0	40.0	33.0	41.0
24	37.500796827462	39.0	35.0	41.0	33.0	41.0
25	36.63954707247579	38.0	35.0	40.0	31.0	41.0
26	36.866837489808844	39.0	35.0	40.0	32.0	41.0
27	37.1218393220859	39.0	35.0	40.0	33.0	41.0
28	37.11532266540917	39.0	35.0	40.0	32.0	41.0
29	37.229688001568086	39.0	35.0	41.0	33.0	41.0
30	36.772641404916186	39.0	35.0	40.0	31.0	41.0
31	37.00783191911846	39.0	35.0	40.0	32.0	41.0
32	36.84647222750915	39.0	35.0	40.0	32.0	41.0
33	36.78531394433855	39.0	35.0	41.0	31.0	41.0
34	36.82239695927232	39.0	35.0	41.0	32.0	41.0
35	36.665892091664986	39.0	35.0	41.0	31.0	41.0
36	36.60106073217223	39.0	35.0	41.0	31.0	41.0
37	36.6020038577244	39.0	35.0	41.0	31.0	41.0
38	36.50164336786726	39.0	35.0	41.0	31.0	41.0
39	36.47593751509143	39.0	35.0	40.0	31.0	41.0
40	36.25070947471884	39.0	35.0	40.0	30.0	41.0
41	36.1376707639601	39.0	35.0	40.0	30.0	41.0
42	36.32265120546786	39.0	35.0	40.0	31.0	41.0
43	36.32828439212433	39.0	35.0	40.0	31.0	41.0
44	36.41233903659157	39.0	35.0	40.0	31.0	41.0
45	36.41043574104954	39.0	35.0	40.0	31.0	41.0
46	36.23502859204422	39.0	35.0	40.0	31.0	41.0
47	36.10574653216712	39.0	35.0	40.0	30.0	41.0
48	36.16829961848867	39.0	35.0	40.0	31.0	41.0
49	36.16072904741478	39.0	35.0	40.0	31.0	41.0
50	36.027773911215526	38.0	35.0	40.0	31.0	41.0
51	35.16010124395988	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	14.0
11	10.0
12	14.0
13	16.0
14	17.0
15	39.0
16	41.0
17	78.0
18	126.0
19	266.0
20	660.0
21	903.0
22	1257.0
23	1649.0
24	2368.0
25	3288.0
26	3859.0
27	4145.0
28	4611.0
29	5367.0
30	6714.0
31	9208.0
32	13368.0
33	21957.0
34	30328.0
35	28709.0
36	42009.0
37	67351.0
38	79613.0
39	24029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03651770775039	17.84126515179492	18.899440658369812	18.222776482084875
2	29.776916718036706	29.109058834558166	22.92817758031481	18.185846867090316
3	31.00894548904753	26.112362614730372	25.99305155090179	16.885640345320308
4	27.4699520767227	23.404853687706133	31.101269526533926	18.023924709037246
5	28.49432278187949	26.895554526576536	24.81357646276785	19.796546228776123
6	23.696597646163156	36.60719104826132	25.755281645129124	13.940929660446393
7	71.86304226168325	6.17377940520594	16.813485559100165	5.149692774010641
8	70.76538047446033	10.835149039403898	11.991330062695123	6.408140423440647
9	65.34894225060437	7.843850224844541	12.656915354481693	14.1502921700694
10	43.10055366015096	21.910624650233935	19.99767059351573	14.991151096099381
11	34.282329747373026	22.64296732297221	24.638586902485933	18.436116027168833
12	29.230926564040217	19.83404399169368	29.788279676496572	21.14674976776954
13	24.774942404004307	24.149979688711753	31.742992605554782	19.33208530172916
14	20.100221293616006	29.532044963226628	27.886404504276737	22.481329238880633
15	18.337542362529508	24.189181895398285	37.14352268756693	20.32975305450527
16	20.419804500299698	24.059928242917326	30.8353762985731	24.68489095820988
17	20.080336116311244	25.401041415142846	29.862707054408688	24.655915414137226
18	20.195386070717372	24.730910939972333	31.46772493686456	23.605978052445735
19	22.833580951136437	26.561767621818017	27.191275520494518	23.41337590655103
20	25.017541567122414	25.76920126924246	31.42000051133313	17.793256652301995
21	23.25060152661347	29.034915530607545	28.09974404936069	19.61473889341829
22	20.8311435965468	23.440078858931713	30.034003653191142	25.694773891330346
23	22.174813434425786	27.173094786958735	29.291718391800487	21.36037338681499
24	22.822786140599565	24.43405365020837	29.385178725132878	23.35798148405919
25	19.75081032097517	29.50818275046091	28.250871396876892	22.49013553168703
26	19.916141366566197	25.9589626755222	30.260694674465444	23.864201283446157
27	21.94300908184455	27.174515156766216	29.196269540737624	21.68620622065161
28	18.08812542433548	26.56148354785652	32.21398723371617	23.13640379409183
29	23.119075282440534	23.721028006851864	30.24450245866014	22.915394252047463
30	19.599114825535978	28.726695282383723	30.7010093147852	20.973180577295107
31	22.421673706966345	25.257584064587057	26.883339346232184	25.437402882214418
32	24.49086844250769	28.99883813749748	26.030265239857847	20.48002818013698
33	21.82511838782345	27.06173779405206	26.008959692745602	25.104184125378882
34	22.45150147292349	24.71528687209002	29.089741805176395	23.743469849810097
35	20.889378758653603	24.56728433815028	28.281551384718522	26.26178551847759
36	22.102942722167143	26.828797145624833	26.62596833711625	24.44229179509177
37	19.087781694842068	27.561139818363113	31.74327667951628	21.60780180727854
38	18.95881211632261	28.821291911562096	25.55273691058204	26.667159061533262
39	22.115441976472994	28.066507395865585	28.324730626866014	21.493320000795407
40	20.756432144673187	25.714943142596606	29.32836393283355	24.200260779896652
41	18.659966308828167	24.74255797239369	28.472733160805745	28.124742557972393
42	19.08352058541962	23.47786069581076	29.553918658261864	27.884700060507754
43	22.117146420241973	24.332923319915572	26.4288210078376	27.121109252004853
44	20.87886802207823	24.594555438453956	28.40796429758452	26.118612241883298
45	20.29594825308717	25.07179969376827	27.126222583311794	27.506029469832765
46	23.792330571187513	27.304621031131663	26.062649671468463	22.840398726212356
47	18.383278270330464	24.816133128421317	33.362782334008486	23.43780626723974
48	20.810974345280535	24.070723053454195	29.422392414088932	25.695910187176334
49	20.064996122390426	22.35406410413015	32.87786808173376	24.703071691745663
50	20.54792185693467	23.25003337869048	29.795665599495486	26.406379164879368
51	19.900801372645383	23.437522193278244	27.274225117251525	29.387451316824848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96.0
1	272.5
2	449.0
3	2160.5
4	3872.0
5	2603.5
6	1335.0
7	1354.5
8	1374.0
9	1329.0
10	1284.0
11	1278.5
12	1273.0
13	1263.0
14	1253.0
15	1235.0
16	1217.0
17	1147.0
18	1077.0
19	1051.5
20	1026.0
21	1008.0
22	990.0
23	1011.5
24	1033.0
25	1565.0
26	2501.0
27	2905.0
28	3323.0
29	3741.0
30	4237.5
31	4734.0
32	5371.5
33	6009.0
34	6948.5
35	7888.0
36	8584.0
37	9280.0
38	10137.5
39	10995.0
40	12337.0
41	13679.0
42	16185.5
43	18692.0
44	20418.5
45	22145.0
46	34368.0
47	46591.0
48	38984.0
49	31377.0
50	31961.5
51	32546.0
52	28755.5
53	24965.0
54	23215.5
55	21466.0
56	19242.5
57	17019.0
58	15850.5
59	14682.0
60	13944.5
61	13207.0
62	11882.0
63	10557.0
64	8901.5
65	7246.0
66	5869.5
67	4493.0
68	4023.5
69	3554.0
70	2758.5
71	1963.0
72	1731.0
73	1499.0
74	1235.5
75	808.5
76	645.0
77	540.0
78	435.0
79	331.0
80	227.0
81	147.5
82	68.0
83	50.0
84	32.0
85	25.5
86	19.0
87	14.0
88	9.0
89	5.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	352021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.199219371289235
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.82621186564091	20.972693168735475
2	11.37578832574986	6.643282776898427
3	3.8934241422797213	3.4105483690769764
4	1.9227460389652253	2.245707335480922
5	1.2204386413038804	1.7817927808315093
6	0.814948280214595	1.4277512166144508
7	0.6571511541330753	1.34318104967393
8	0.5522865408027312	1.2901068688567545
9	0.4763845869793234	1.2519052254279255
>10	6.996961030272157	45.60370244055793
>50	0.21072777296194659	3.839409311302594
>100	0.04094710280303227	2.064345930025145
>500	0.003994839297856807	0.9739956782432526
>1k	0.004993549122321009	2.2821826880035014
>5k	0.0029961294733926052	4.869395160271207
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTC	5802	1.6481971246033618	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	5505	1.563827158038867	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGC	5391	1.5314427264282529	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2109	0.5991119847963615	No Hit
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	2038	0.578942733530102	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCAATCCT	1476	0.4192931671690041	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	1118	0.31759468895321585	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCT	1085	0.30822024822382754	No Hit
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	978	0.2778243343436897	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCAATCCTCGTA	925	0.2627684143843691	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTC	808	0.22953176088926514	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	629	0.17868252178137098	TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCC	434	0.12328809928953102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1843640010113033	0.0
2	0.0	0.0	0.0	1.2340172887412968	0.0
3	0.0	0.0	0.0	1.5152505106229457	0.0
4	0.0	0.0	0.0	2.189926169177407	0.0
5	0.0	0.0	0.0	4.797725135716335	0.0
6	0.0	0.0	0.0	5.376383795284941	0.0
7	0.0	0.0	0.0	6.236275676735195	0.0
8	0.0	0.0	0.0	7.236216021203281	0.0
9	0.0	0.0	0.0	7.551538118464523	0.0
10	0.0	0.0	0.0	9.938043468997588	0.0
11	0.0	0.0	0.0	11.199047784081063	0.0
12	0.0	0.0	0.0	13.73469196439985	0.0
13	0.0	0.0	0.0	14.242048059632806	0.0
14	0.0	0.0	0.0	14.46334167563867	0.0
15	0.0	0.0	0.0	15.02722848920945	0.0
16	0.0	0.0	0.0	15.676621565190713	0.0
17	0.0	0.0	0.0	16.387658690816743	0.0
18	0.0	0.0	0.0	17.09187804136685	0.0
19	0.0	0.0	0.0	18.01739100792282	0.0
20	0.0	0.0	0.0	18.56991486303374	0.0
21	0.0	0.0	0.0	19.152550558063297	0.0
22	0.0	0.0	0.0	19.795978080853132	0.0
23	0.0	0.0	0.0	20.414123021069766	0.0
24	0.0	0.0	0.0	20.934546518531565	0.0
25	0.0	0.0	0.0	21.379974490158258	0.0
26	0.0	0.0	0.0	21.788188772828892	0.0
27	0.0	0.0	0.0	22.247252294607424	0.0
28	0.0	0.0	0.0	22.65461435539357	0.0
29	0.0	0.0	0.0	23.099758253058766	0.0
30	0.0	0.0	0.0	23.586661023063964	0.0
31	0.0	0.0	0.0	24.03350936449814	0.0
32	0.0	0.0	0.0	24.457915862974083	0.0
33	0.0	0.0	0.0	24.881754213527035	0.0
34	0.0	0.0	0.0	25.292809235812637	0.0
35	0.0	0.0	0.0	25.748463870053207	0.0
36	0.0	0.0	0.0	26.182812957181532	0.0
37	0.0	0.0	0.0	26.618866488078837	0.0
38	0.0	0.0	0.0	27.058044832552604	0.0
39	0.0	0.0	0.0	27.506029469832765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	45	3.8380676E-10	45.0	2
ACGGGTC	20	7.0266123E-4	45.0	5
TGCGCGT	20	7.0266123E-4	45.0	28
GTGCTTA	20	7.0266123E-4	45.0	39
TACGAGA	20	7.0266123E-4	45.0	34
CGCTAAT	20	7.0266123E-4	45.0	19
CAGTCTA	20	7.0266123E-4	45.0	17
TAGGCAC	20	7.0266123E-4	45.0	38
AGTAGCG	20	7.0266123E-4	45.0	1
GTAGTAA	20	7.0266123E-4	45.0	40
GTTGCGC	20	7.0266123E-4	45.0	26
CCCGAAA	20	7.0266123E-4	45.0	36
TTAATCG	30	2.1611959E-6	44.999996	20
GTTAGCG	25	3.8852748E-5	44.999996	1
TCGATCT	25	3.8852748E-5	44.999996	33
ACGGCCT	30	2.1611959E-6	44.999996	8
ATACGAG	25	3.8852748E-5	44.999996	1
ATCGATC	25	3.8852748E-5	44.999996	32
TAACTCG	25	3.8852748E-5	44.999996	1
TAATCGT	30	2.1611959E-6	44.999996	21
>>END_MODULE