FastQCFastQC Report
Sat 18 Jun 2016
SRR3551809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345146
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG52251.513852108962584No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC47931.3886876857909407No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC45451.3168340354516639No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC19260.5580247199735764TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16800.4867505345563906No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT13410.3885312302619761No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT12130.35144547524815584No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT8330.241347140050877No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC8030.2326551662195129TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC7040.2039716525760113No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA6750.19556941120569266No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT4610.13356666454196195TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC4320.1251644231716433No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4040.11705191426237013No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3560.10314475613218754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA253.8851133E-545.00000441
TCGATAG351.2086275E-745.0000041
TAGGACG302.1610704E-645.0000041
TATACGG351.2086275E-745.0000042
ATACCTC253.8851133E-545.00000413
ATACCGG302.1610704E-645.0000042
TGCGTTG302.1610704E-645.0000041
CTAGATT207.02642E-445.014
AAGTACG406.7921064E-945.01
ACGTTGA207.02642E-445.011
AATGCGT207.02642E-445.039
TCGCTTG207.02642E-445.01
TATTACG207.02642E-445.028
TTAACGG207.02642E-445.02
CGTGTGA207.02642E-445.043
TTACGGT207.02642E-445.030
CAATCCG207.02642E-445.025
GGCCTAT207.02642E-445.08
GCTTTAA207.02642E-445.033
AGTACGG650.044.9999962