##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	345146
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.43814211956679	28.0	25.0	31.0	16.0	33.0
2	27.637463566143023	31.0	25.0	33.0	16.0	34.0
3	27.586418501156032	31.0	25.0	33.0	16.0	34.0
4	31.69633430490285	35.0	28.0	37.0	19.0	37.0
5	33.200561501509505	35.0	32.0	35.0	28.0	37.0
6	33.00050123715761	35.0	32.0	35.0	28.0	37.0
7	34.03182131619663	35.0	33.0	37.0	31.0	37.0
8	33.87580907789747	35.0	35.0	37.0	30.0	37.0
9	36.16585734732548	39.0	35.0	39.0	31.0	39.0
10	35.442091173010844	37.0	34.0	39.0	31.0	39.0
11	35.99758363127488	37.0	35.0	39.0	31.0	39.0
12	35.9984760072549	37.0	35.0	39.0	31.0	39.0
13	35.98477745649667	37.0	35.0	39.0	31.0	39.0
14	36.87017088420552	39.0	36.0	40.0	31.0	41.0
15	37.10570309376322	39.0	36.0	40.0	32.0	41.0
16	36.67281092639057	38.0	35.0	40.0	32.0	41.0
17	36.851940917756544	38.0	35.0	40.0	32.0	41.0
18	36.669290676988865	38.0	35.0	40.0	31.0	41.0
19	36.19978791583851	38.0	34.0	40.0	30.0	41.0
20	36.58737461827748	38.0	35.0	40.0	31.0	41.0
21	36.81426121119758	38.0	35.0	40.0	32.0	41.0
22	37.18680210693446	39.0	35.0	40.0	32.0	41.0
23	37.5336698092981	39.0	35.0	40.0	33.0	41.0
24	37.55962404315855	39.0	35.0	41.0	33.0	41.0
25	36.6061405897794	38.0	35.0	40.0	31.0	41.0
26	36.88867899381711	39.0	35.0	40.0	32.0	41.0
27	37.129327878636865	39.0	35.0	40.0	32.0	41.0
28	37.16774930029611	39.0	35.0	40.0	32.0	41.0
29	37.274466457672986	39.0	35.0	41.0	33.0	41.0
30	36.919008187839346	39.0	35.0	40.0	32.0	41.0
31	37.050636542216914	39.0	35.0	40.0	32.0	41.0
32	36.916638176308	39.0	35.0	40.0	32.0	41.0
33	36.94866230522735	39.0	35.0	41.0	32.0	41.0
34	36.94297485701703	39.0	35.0	41.0	32.0	41.0
35	36.82533188853413	39.0	35.0	41.0	31.0	41.0
36	36.73324332311544	39.0	35.0	41.0	31.0	41.0
37	36.717360189600925	39.0	35.0	41.0	31.0	41.0
38	36.625126757951705	39.0	35.0	41.0	31.0	41.0
39	36.623686787620315	39.0	35.0	41.0	31.0	41.0
40	36.37660004751612	39.0	35.0	40.0	30.0	41.0
41	36.25461978409137	39.0	35.0	40.0	30.0	41.0
42	36.36421398480643	39.0	35.0	40.0	31.0	41.0
43	36.373720106853334	39.0	35.0	40.0	31.0	41.0
44	36.49557288799522	39.0	35.0	40.0	31.0	41.0
45	36.521622733567824	39.0	35.0	40.0	32.0	41.0
46	36.34986643333546	39.0	35.0	40.0	31.0	41.0
47	36.24410829040464	39.0	35.0	40.0	31.0	41.0
48	36.263897017494045	38.0	35.0	40.0	31.0	41.0
49	36.224044317477244	38.0	35.0	40.0	31.0	41.0
50	36.11194682829875	38.0	35.0	40.0	31.0	41.0
51	35.17739449392431	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	9.0
10	24.0
11	23.0
12	22.0
13	17.0
14	25.0
15	37.0
16	53.0
17	95.0
18	161.0
19	365.0
20	618.0
21	862.0
22	1165.0
23	1531.0
24	2164.0
25	2953.0
26	3514.0
27	3862.0
28	4232.0
29	4832.0
30	6426.0
31	8599.0
32	13033.0
33	21334.0
34	29077.0
35	29417.0
36	41949.0
37	66472.0
38	79255.0
39	23018.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61590167639201	17.49636385761388	20.8340818088577	17.0536526571364
2	29.50374624072132	28.613977852850674	23.205252270053833	18.67702363637417
3	30.987176441274126	26.151251933964176	26.315819971838007	16.54575165292369
4	27.774043448279855	23.685918422928268	30.73221187555411	17.80782625323776
5	29.615582970684866	26.507622861050105	24.49687958139454	19.379914586870484
6	24.90685101377388	36.38257433086288	24.318694117851575	14.391880537511662
7	72.57073818036424	6.369478423623626	15.439842849113127	5.619940546898993
8	71.06152179077839	11.026927734929567	11.411402710736905	6.500147763555134
9	65.92340632659803	8.552322785140202	12.547153958035151	12.977116930226629
10	42.65673077480255	23.275077793165792	18.97255074664055	15.09564068539111
11	33.80395542755819	23.59697055738731	24.99724754162007	17.601826473434432
12	31.522602029286155	19.858842344978648	28.38566867354685	20.232886952188352
13	25.44430472901323	25.87281903889948	29.95428021764702	18.728596014440267
14	19.54390315982222	30.498397779490418	27.25368394824219	22.70401511244517
15	17.83013565273826	24.813267428856197	37.424452260782395	19.93214465762315
16	19.724696215514594	24.284795419909255	31.88187028098254	24.108638083593608
17	19.24374033017911	25.76416936600743	29.922409646932024	25.069680656881438
18	20.276057088884127	26.307417730467687	30.35584940865605	23.06067577199214
19	23.698376918753222	26.855591546765716	25.942354829550396	23.50367670493067
20	26.729847658671986	25.970748610732848	29.799273350987697	17.50013037960747
21	23.956818274005784	28.415221384573485	27.424336367797974	20.203623973622754
22	21.155395108157126	25.16992808840317	28.591378720889132	25.08329808255057
23	22.831497395305174	28.273542211122248	27.800698834696043	21.094261558876536
24	23.672880462181222	24.62870785117023	28.10202059418333	23.59639109246522
25	20.195511464713483	30.085529022500623	27.049422563205137	22.669536949580756
26	21.103822730091036	28.18836086757488	28.688439095339362	22.019377306994723
27	23.25016080151588	29.42580820870009	26.536596107154654	20.78743488262938
28	18.99312175137478	28.081449589449104	31.950536874250318	20.974891784925802
29	23.741257322987952	25.66131434233629	28.87589599763578	21.721532337039978
30	20.76048976375215	30.738006524775024	27.7392755529544	20.762228158518425
31	23.96608971275924	26.710435583781937	26.101417950664356	23.22205675279447
32	26.053612094591855	30.907789746947667	24.53715239347986	18.501445764980616
33	24.18802477792007	29.54257039050141	23.55669774530199	22.71270708627653
34	25.250183980112762	27.15140838949314	26.305099870779326	21.29330775961477
35	22.83323579007145	27.18269949528605	26.664657854936753	23.319406859705747
36	25.298859033568405	28.904000046357194	25.95278519814803	19.844355721926373
37	21.77252525018398	31.47218858106425	28.649325213098226	18.10596095565355
38	21.230435815567905	29.301802715372627	26.73448337804871	22.73327809101076
39	25.603947315049286	28.125488923528014	26.468219246347925	19.80234451507478
40	22.997224363023182	26.74404454926321	29.57415122875537	20.684579858958237
41	23.09660259716178	26.52268894902447	27.176615113604097	23.20409334020965
42	21.746449328689888	24.830651376518922	30.682667624715336	22.740231670075854
43	25.712307255480287	25.630023236543376	25.83689221372984	22.82077729424649
44	22.545821188714342	27.071152497783547	27.23340267596901	23.1496236375331
45	21.376460975934823	26.356092783923323	26.857329941531987	25.410116298609864
46	24.796173213654512	28.842287032154506	25.211649562793713	21.149890191397265
47	20.785696487863106	26.80228077393335	31.639943676009572	20.77207906219397
48	23.4599271033128	25.063306542738435	28.322506997038932	23.15425935690983
49	21.069054834765577	24.303917762338255	32.090767385396326	22.53626001749984
50	21.634612598726335	25.749682742955155	28.399575831677033	24.216128826641476
51	21.032838277134893	25.05461456890707	26.100259020820175	27.81228813313786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	182.0
1	332.0
2	482.0
3	1790.5
4	3099.0
5	2207.5
6	1316.0
7	1249.5
8	1183.0
9	1174.5
10	1166.0
11	1151.5
12	1137.0
13	1050.5
14	964.0
15	1023.5
16	1083.0
17	1049.0
18	1015.0
19	918.5
20	822.0
21	1008.0
22	1194.0
23	1190.5
24	1187.0
25	1708.0
26	2593.5
27	2958.0
28	3734.0
29	4510.0
30	5222.0
31	5934.0
32	6240.5
33	6547.0
34	7392.5
35	8238.0
36	9118.5
37	9999.0
38	10743.5
39	11488.0
40	13425.5
41	15363.0
42	17797.5
43	20232.0
44	32449.0
45	44666.0
46	37967.0
47	31268.0
48	30253.0
49	29238.0
50	25738.5
51	22239.0
52	20536.0
53	18833.0
54	17254.0
55	15675.0
56	15705.5
57	15736.0
58	15186.0
59	14636.0
60	14649.0
61	14662.0
62	12829.5
63	10997.0
64	9415.0
65	7833.0
66	6609.5
67	5386.0
68	4624.5
69	3863.0
70	3214.5
71	2566.0
72	2262.0
73	1958.0
74	1718.5
75	1114.0
76	749.0
77	653.5
78	558.0
79	400.0
80	242.0
81	187.0
82	132.0
83	87.0
84	42.0
85	27.5
86	13.0
87	21.0
88	29.0
89	20.5
90	12.0
91	9.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	345146.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.958469749033743
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.80163883580626	21.371825256558093
2	10.48434701697866	6.0722129185909735
3	3.56982060851034	3.1012962630307177
4	1.7048694834365528	1.9748164544859277
5	1.0965592452150597	1.587733886529179
6	0.7533842259552371	1.3090112590034362
7	0.5842979919759077	1.1844263007538838
8	0.48924951725379945	1.133433387609881
9	0.4242163503386727	1.105619071349516
>10	6.640386597164554	44.79582553470126
>50	0.3851964501895967	6.634004160558141
>100	0.05502806431279953	2.852126346531613
>500	0.0040020410409308755	0.8735433700520939
>1k	0.006003061561396312	4.490273681282703
>5k	0.0010005102602327189	1.513852108962584
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	5225	1.513852108962584	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGC	4793	1.3886876857909407	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	4545	1.3168340354516639	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1926	0.5580247199735764	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1680	0.4867505345563906	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATAAGTGT	1341	0.3885312302619761	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	1213	0.35144547524815584	No Hit
GAACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	833	0.241347140050877	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	803	0.2326551662195129	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTC	704	0.2039716525760113	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATAAGTGTCGTA	675	0.19556941120569266	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	461	0.13356666454196195	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	432	0.1251644231716433	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	404	0.11705191426237013	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	356	0.10314475613218754	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13878184884078043	0.0
2	0.0	0.0	0.0	1.1085163959599706	0.0
3	0.0	0.0	0.0	1.3739113302776216	0.0
4	0.0	0.0	0.0	1.958881169128427	0.0
5	0.0	0.0	0.0	4.2045974746918695	0.0
6	0.0	0.0	0.0	4.759435137593946	0.0
7	0.0	0.0	0.0	5.602556599236265	0.0
8	0.0	0.0	0.0	6.509129469847543	0.0
9	0.0	0.0	0.0	6.796544071204649	0.0
10	0.0	0.0	0.0	8.996192915461863	0.0
11	0.0	0.0	0.0	10.025032884634328	0.0
12	0.0	0.0	0.0	12.224391996430496	0.0
13	0.0	0.0	0.0	12.650878179089428	0.0
14	0.0	0.0	0.0	12.850793577210803	0.0
15	0.0	0.0	0.0	13.318132036877147	0.0
16	0.0	0.0	0.0	13.884269265759997	0.0
17	0.0	0.0	0.0	14.49560475856594	0.0
18	0.0	0.0	0.0	15.126642058722975	0.0
19	0.0	0.0	0.0	15.941080006721792	0.0
20	0.0	0.0	0.0	16.42493321666773	0.0
21	0.0	0.0	0.0	16.93602127795194	0.0
22	0.0	0.0	0.0	17.526785766023654	0.0
23	0.0	0.0	0.0	18.062790818957776	0.0
24	0.0	0.0	0.0	18.48637967700625	0.0
25	0.0	0.0	0.0	18.869985455430456	0.0
26	0.0	0.0	0.0	19.21302868930829	0.0
27	0.0	0.0	0.0	19.576932660381402	0.0
28	0.0	0.0	0.0	19.95068753513006	0.0
29	0.0	0.0	0.0	20.306189264832852	0.0
30	0.0	0.0	0.0	20.733834377335967	0.0
31	0.0	0.0	0.0	21.134244638500807	0.0
32	0.0	0.0	0.0	21.532916504899376	0.0
33	0.0	0.0	0.0	21.91565308594044	0.0
34	0.0	0.0	0.0	22.28737983346178	0.0
35	0.0	0.0	0.0	22.706043239672486	0.0
36	0.0	0.0	0.0	23.16382052812433	0.0
37	0.0	0.0	0.0	23.580745539568763	0.0
38	0.0	0.0	0.0	23.97680981381792	0.0
39	0.0	0.0	0.0	24.34795709641717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCGA	25	3.8851133E-5	45.000004	41
TCGATAG	35	1.2086275E-7	45.000004	1
TAGGACG	30	2.1610704E-6	45.000004	1
TATACGG	35	1.2086275E-7	45.000004	2
ATACCTC	25	3.8851133E-5	45.000004	13
ATACCGG	30	2.1610704E-6	45.000004	2
TGCGTTG	30	2.1610704E-6	45.000004	1
CTAGATT	20	7.02642E-4	45.0	14
AAGTACG	40	6.7921064E-9	45.0	1
ACGTTGA	20	7.02642E-4	45.0	11
AATGCGT	20	7.02642E-4	45.0	39
TCGCTTG	20	7.02642E-4	45.0	1
TATTACG	20	7.02642E-4	45.0	28
TTAACGG	20	7.02642E-4	45.0	2
CGTGTGA	20	7.02642E-4	45.0	43
TTACGGT	20	7.02642E-4	45.0	30
CAATCCG	20	7.02642E-4	45.0	25
GGCCTAT	20	7.02642E-4	45.0	8
GCTTTAA	20	7.02642E-4	45.0	33
AGTACGG	65	0.0	44.999996	2
>>END_MODULE