FastQCFastQC Report
Sat 18 Jun 2016
SRR3551807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541359
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG68971.2740159487511984No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC68521.265703534992491No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC68441.2642257725464987No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC27390.5059489174466482TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.3930848106339786No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT18370.339331201661005No Hit
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT16390.30275658112269305TruSeq Adapter, Index 8 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT15390.28428455054778806No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA14380.265627799667134No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC11280.20836450488492847No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC7840.14482071970725527TruSeq Adapter, Index 13 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6680.12339316424036545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCGA207.0299755E-445.01
GATCGTT406.8030204E-945.09
CCGATTT207.0299755E-445.012
GTTTACG253.8880622E-545.01
TCGATTG253.8880622E-545.01
CTACGAA1050.045.011
CGAATAT1050.045.014
GCTAGCG453.8380676E-1045.01
CGTAAGG551.8189894E-1245.02
CAGTCGA253.8880622E-545.024
GACGCAA253.8880622E-545.09
GCTACGA1050.045.010
TTCGCGA207.0299755E-445.030
CGTCCAA207.0299755E-445.023
GCGTCAT207.0299755E-445.037
CGCCCGT207.0299755E-445.043
TTCTCGT207.0299755E-445.013
CGAACCC302.163366E-644.99999634
ACTACGG302.163366E-644.9999962
TACGGCT7850.042.133767
Produced by FastQC (version 0.11.5)