FastQCFastQC Report
Sat 18 Jun 2016
SRR3551803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374670
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC68181.8197346998692183No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG66111.7644860810846879No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC56531.5087944057437213No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC30170.80524194624603TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20030.5346037846638375No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC17720.4729495289187819TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT15470.4128966824138576No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT14020.37419595911068404No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT12820.3421677743080578No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA10480.27971281394293646No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC9540.25462406918087915No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT8820.23540715829930337TruSeq Adapter, Index 14 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6640.17722262257453225No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC6320.1686817732938319TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC5410.1443937331518403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG302.1615706E-645.0000041
CGACAGG302.1615706E-645.0000042
GTAATAG253.8857546E-545.0000041
AACGGGC302.1615706E-645.0000044
TGCGACG253.8857546E-545.0000041
CGAGGGT302.1615706E-645.0000044
GCGGCAC253.8857546E-545.00000426
ACTTTGC253.8857546E-545.00000438
CACCGGG302.1615706E-645.0000043
TGTCGGA253.8857546E-545.00000420
TCAACGG253.8857546E-545.0000042
AACCGCC207.027193E-445.033
ATGTGCG207.027193E-445.012
AGTATGC207.027193E-445.036
TAGCACG207.027193E-445.01
GTAGCTA207.027193E-445.016
TACGACG207.027193E-445.01
TACTCAC453.8380676E-1045.030
TTAGTTA207.027193E-445.013
AATTACG207.027193E-445.02
Produced by FastQC (version 0.11.5)