##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	374670
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.60586916486508	28.0	25.0	31.0	16.0	33.0
2	27.628110070195106	31.0	25.0	33.0	16.0	34.0
3	27.584567752955934	31.0	25.0	33.0	16.0	34.0
4	31.6658606240158	35.0	28.0	35.0	19.0	37.0
5	33.26053060026156	35.0	32.0	35.0	28.0	37.0
6	33.188483198548056	35.0	32.0	35.0	28.0	37.0
7	33.97896015160008	35.0	33.0	37.0	31.0	37.0
8	33.950038700723304	35.0	35.0	37.0	30.0	37.0
9	36.06355993274081	39.0	35.0	39.0	30.0	39.0
10	35.4048309178744	37.0	34.0	39.0	31.0	39.0
11	35.99809699201965	37.0	35.0	39.0	31.0	39.0
12	35.91694024074519	37.0	35.0	39.0	31.0	39.0
13	35.95802706381616	37.0	35.0	39.0	31.0	39.0
14	36.838073504684125	39.0	36.0	40.0	31.0	41.0
15	37.05718632396509	39.0	35.0	40.0	32.0	41.0
16	36.51034777270665	38.0	34.0	40.0	31.0	41.0
17	36.76263111538154	38.0	35.0	40.0	32.0	41.0
18	36.5073584754584	38.0	35.0	40.0	31.0	41.0
19	35.99326874316065	37.0	34.0	40.0	30.0	41.0
20	36.48240318146636	38.0	35.0	40.0	31.0	41.0
21	36.72721061200523	38.0	35.0	40.0	32.0	41.0
22	37.14657164971842	39.0	35.0	40.0	32.0	41.0
23	37.47383564203165	39.0	35.0	40.0	33.0	41.0
24	37.47207409186751	39.0	35.0	41.0	33.0	41.0
25	36.563858862465636	38.0	35.0	40.0	31.0	41.0
26	36.727130541543225	38.0	35.0	40.0	31.0	41.0
27	37.02603090719833	39.0	35.0	40.0	32.0	41.0
28	37.018365494968904	39.0	35.0	40.0	32.0	41.0
29	37.01683348012918	39.0	35.0	40.0	32.0	41.0
30	36.72257186323965	39.0	35.0	40.0	31.0	41.0
31	36.887103317586146	39.0	35.0	40.0	32.0	41.0
32	36.723578082045535	39.0	35.0	40.0	31.0	41.0
33	36.779461926495316	39.0	35.0	40.0	31.0	41.0
34	36.64317399311394	39.0	35.0	40.0	31.0	41.0
35	36.54664104411882	39.0	35.0	41.0	31.0	41.0
36	36.48176261777031	39.0	35.0	41.0	31.0	41.0
37	36.625171484239466	39.0	35.0	41.0	31.0	41.0
38	36.565321482904956	39.0	35.0	40.0	31.0	41.0
39	36.46923959751248	39.0	35.0	40.0	31.0	41.0
40	36.28840846611685	39.0	35.0	40.0	30.0	41.0
41	36.0707315771212	38.0	35.0	40.0	30.0	41.0
42	36.22521151947047	39.0	35.0	40.0	31.0	41.0
43	36.22625243547655	39.0	35.0	40.0	31.0	41.0
44	36.36195051645448	39.0	35.0	40.0	31.0	41.0
45	36.390340833266606	39.0	35.0	40.0	31.0	41.0
46	36.20647236234553	39.0	35.0	40.0	31.0	41.0
47	36.08520030960579	38.0	35.0	40.0	30.0	41.0
48	36.1414711612886	38.0	35.0	40.0	31.0	41.0
49	36.15699148584088	38.0	35.0	40.0	31.0	41.0
50	36.04970507379828	38.0	35.0	40.0	31.0	41.0
51	35.1073664825046	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	7.0
11	13.0
12	18.0
13	22.0
14	29.0
15	28.0
16	39.0
17	85.0
18	134.0
19	273.0
20	493.0
21	911.0
22	1367.0
23	1892.0
24	2468.0
25	3316.0
26	3862.0
27	4242.0
28	4790.0
29	5627.0
30	7287.0
31	10059.0
32	15006.0
33	23959.0
34	32745.0
35	33083.0
36	47011.0
37	72346.0
38	81140.0
39	22407.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56118717805002	17.560253022659943	19.91939573491339	19.95916406437665
2	28.44316331705234	29.618330797768706	22.980756398964424	18.95774948621454
3	28.27421464221849	26.617556783302636	27.69210238343075	17.41612619104812
4	27.782848907038193	22.637521018496276	32.06501721514933	17.5146128593162
5	28.449035150932822	24.88509888702058	25.939359970107027	20.72650599193957
6	23.72888141564577	34.22238236314624	26.118450903461714	15.930285317746284
7	69.95275842741613	5.99620999813169	17.792724264019004	6.258307310433181
8	68.23524701737529	10.882643392852378	13.08244588571276	7.799663704059573
9	64.15779219046094	7.58133824432167	14.072116796113916	14.188752769103477
10	41.84615795233139	21.635038834174072	20.912536365334827	15.606266848159715
11	33.091787439613526	22.46803854058238	25.427976619425095	19.012197400378998
12	29.87295486694958	18.7634451650786	29.998131689219846	21.36546827875197
13	24.34489017001628	24.03875410361118	32.34873355219259	19.267622174179948
14	18.164251207729468	29.539061040382204	28.230442789654898	24.066244962233434
15	16.483839111751674	24.035551285130914	39.29938345744255	20.18122614567486
16	18.265406891397763	23.29783542851042	32.82728801345184	25.609469666639978
17	18.099927936584194	24.628873408599567	30.874102543571674	26.39709611124456
18	18.695919075453066	25.59132036191849	31.080417434008595	24.63234312861985
19	21.550964849067178	26.011156484372915	27.423599434168732	25.014279232391175
20	24.607254383857796	24.85600661915819	32.20700883444098	18.329730162543036
21	22.51367870392612	28.094589905783756	29.100008007046203	20.29172338324392
22	19.946619691995622	24.604852269997597	28.550991539221183	26.897536498785595
23	20.879707475912134	27.16630634958764	28.96175300931486	22.992233165185365
24	23.22950863426482	23.24365441588598	28.871807190327488	24.655029759521714
25	18.605439453385646	29.808898497344327	27.209544399071184	24.376117650198843
26	18.40392879066912	27.233298636133128	29.862011903808682	24.500760669389063
27	22.426401900338966	27.62377558918515	26.2719192889743	23.67790322150159
28	16.534817305895857	27.60989670910401	31.685750126778235	24.169535858221902
29	20.191368404195693	24.048095657511944	30.49403475058051	25.266501187711853
30	18.553126751541356	26.561507459898042	28.11754343822564	26.767822350334963
31	22.343128619852138	25.635892919102144	24.56428323591427	27.45669522513145
32	22.92817679558011	27.325646568980705	27.300557824218647	22.44561881122054
33	21.688952945258492	23.45744254944351	24.96890597058745	29.884698534710548
34	23.203619184882697	25.298796274054503	26.995756265513656	24.50182827554915
35	18.997517815677796	23.143032535297728	29.539327941922227	28.320121707102253
36	21.076680812448288	25.642832359142716	27.973149705073798	25.3073371233352
37	20.34803960818854	23.712600421704433	33.0109162729869	22.928443697120134
38	18.439693597032054	24.2402647663277	27.16977606960792	30.15026556703232
39	23.31304881629167	23.384578429017537	30.77081164758321	22.531561107107585
40	21.62035924947287	22.411188512557718	31.240291456481707	24.72816078148771
41	20.79136306616489	23.922118130621612	29.629807563989647	25.65671123922385
42	19.982384498358556	22.177382763498546	32.75575840072597	25.08447433741693
43	21.807724130568236	23.208690314143112	29.072250246883925	25.91133530840473
44	21.325433047748685	23.034937411588867	28.652147222889475	26.987482317772976
45	20.439053033336002	22.031921424186617	26.89086396028505	30.638161582192332
46	23.456908746363467	25.82512611097766	26.963461179171006	23.75450396348787
47	18.02733071769824	23.25646568980703	34.63207622707983	24.084127365414897
48	20.91440467611498	21.7548776256439	29.36504123628793	27.965676461953187
49	19.41842154429231	19.904449248672165	35.06312221421518	25.61400699282035
50	20.266901540021887	21.461285931619827	31.346785170950437	26.925027357407856
51	19.121627031787973	21.89660234339552	27.280273307176984	31.70149731763952
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	153.0
1	357.5
2	562.0
3	2095.5
4	3629.0
5	2555.5
6	1482.0
7	1431.5
8	1381.0
9	1356.5
10	1332.0
11	1315.0
12	1298.0
13	1260.0
14	1222.0
15	1179.5
16	1137.0
17	1137.5
18	1138.0
19	1044.5
20	951.0
21	925.0
22	899.0
23	1111.0
24	1323.0
25	1632.5
26	2441.5
27	2941.0
28	3402.5
29	3864.0
30	4373.0
31	4882.0
32	5712.0
33	6542.0
34	7685.0
35	8828.0
36	8620.0
37	8412.0
38	9180.5
39	9949.0
40	11632.0
41	13315.0
42	15442.0
43	17569.0
44	21130.0
45	24691.0
46	39537.5
47	54384.0
48	45059.0
49	35734.0
50	35263.0
51	34792.0
52	29963.0
53	25134.0
54	22639.5
55	20145.0
56	18675.0
57	17205.0
58	16210.0
59	15215.0
60	14373.0
61	13531.0
62	12415.5
63	11300.0
64	9872.0
65	8444.0
66	6788.0
67	5132.0
68	4549.0
69	3966.0
70	3375.0
71	2784.0
72	2588.5
73	2393.0
74	2082.0
75	1598.5
76	1426.0
77	1133.0
78	840.0
79	681.5
80	523.0
81	389.5
82	256.0
83	195.0
84	134.0
85	97.0
86	60.0
87	47.0
88	34.0
89	24.0
90	14.0
91	10.0
92	6.0
93	4.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	374670.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.95214429856252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.21009265615746	19.345704968417955
2	12.65648446191652	7.075517599840101
3	4.724618633560182	3.9618966540285445
4	2.2996076115243125	2.571158551496012
5	1.3376719965535295	1.8695400335905246
6	0.9939079644498203	1.6669115305075157
7	0.6722166795291107	1.3152928338268781
8	0.5517133728411678	1.233725744728236
9	0.5278120581624466	1.3278130931050185
>10	6.643459848411731	41.959165860109884
>50	0.31569131576905357	5.5111194291082874
>100	0.05178532624602771	2.4677277370969275
>500	0.00497935829288728	1.0224437651840965
>1k	0.006971101610042191	3.3601738877041187
>5k	0.0029876149757323677	5.311808311255902
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTC	6818	1.8197346998692183	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCG	6611	1.7644860810846879	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGC	5653	1.5087944057437213	No Hit
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3017	0.80524194624603	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2003	0.5346037846638375	No Hit
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	1772	0.4729495289187819	TruSeq Adapter, Index 15 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCTCGTTT	1547	0.4128966824138576	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCT	1402	0.37419595911068404	No Hit
GAACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCT	1282	0.3421677743080578	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTA	1048	0.27971281394293646	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTC	954	0.25462406918087915	No Hit
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	882	0.23540715829930337	TruSeq Adapter, Index 14 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	664	0.17722262257453225	No Hit
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	632	0.1686817732938319	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCC	541	0.1443937331518403	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24314730295993808	0.0
2	0.0	0.0	0.0	1.855232604692129	0.0
3	0.0	0.0	0.0	2.2761363333066433	0.0
4	2.6690154002188593E-4	0.0	0.0	3.1902741078816024	0.0
5	2.6690154002188593E-4	0.0	0.0	6.45207782848907	0.0
6	2.6690154002188593E-4	0.0	0.0	7.353137427602957	0.0
7	2.6690154002188593E-4	0.0	0.0	8.66549229989057	0.0
8	2.6690154002188593E-4	0.0	0.0	10.021885926281795	0.0
9	2.6690154002188593E-4	0.0	0.0	10.47828755971922	0.0
10	2.6690154002188593E-4	0.0	0.0	13.233245255825127	0.0
11	2.6690154002188593E-4	0.0	0.0	14.75218192008968	0.0
12	2.6690154002188593E-4	0.0	0.0	17.503936797715323	0.0
13	2.6690154002188593E-4	0.0	0.0	18.12955400752662	0.0
14	2.6690154002188593E-4	0.0	0.0	18.39965836602877	0.0
15	2.6690154002188593E-4	0.0	0.0	19.068513625323618	0.0
16	2.6690154002188593E-4	0.0	0.0	19.846264712947395	0.0
17	2.6690154002188593E-4	0.0	0.0	20.64109749913257	0.0
18	2.6690154002188593E-4	0.0	0.0	21.502121867243176	0.0
19	2.6690154002188593E-4	0.0	0.0	22.47524488216297	0.0
20	2.6690154002188593E-4	0.0	0.0	23.091520538073503	0.0
21	2.6690154002188593E-4	0.0	0.0	23.803346945311873	0.0
22	2.6690154002188593E-4	0.0	0.0	24.573357888275016	0.0
23	2.6690154002188593E-4	0.0	0.0	25.309739237195398	0.0
24	2.6690154002188593E-4	0.0	0.0	25.861958523500682	0.0
25	2.6690154002188593E-4	0.0	0.0	26.378946806523075	0.0
26	2.6690154002188593E-4	0.0	0.0	26.85803507086236	0.0
27	2.6690154002188593E-4	0.0	0.0	27.335255024421492	0.0
28	2.6690154002188593E-4	0.0	0.0	27.78098059625804	0.0
29	2.6690154002188593E-4	0.0	0.0	28.231777297355006	0.0
30	2.6690154002188593E-4	0.0	0.0	28.71620359249473	0.0
31	2.6690154002188593E-4	0.0	0.0	29.21611017695572	0.0
32	2.6690154002188593E-4	0.0	0.0	29.713614647556515	0.0
33	2.6690154002188593E-4	0.0	0.0	30.156137400912804	0.0
34	2.6690154002188593E-4	0.0	0.0	30.606667200469747	0.0
35	2.6690154002188593E-4	0.0	0.0	31.103104064910454	0.0
36	2.6690154002188593E-4	0.0	0.0	31.601676141671337	0.0
37	2.6690154002188593E-4	0.0	0.0	32.05300664584835	0.0
38	2.6690154002188593E-4	0.0	0.0	32.4683054421224	0.0
39	2.6690154002188593E-4	0.0	0.0	32.87746550297595	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	30	2.1615706E-6	45.000004	1
CGACAGG	30	2.1615706E-6	45.000004	2
GTAATAG	25	3.8857546E-5	45.000004	1
AACGGGC	30	2.1615706E-6	45.000004	4
TGCGACG	25	3.8857546E-5	45.000004	1
CGAGGGT	30	2.1615706E-6	45.000004	4
GCGGCAC	25	3.8857546E-5	45.000004	26
ACTTTGC	25	3.8857546E-5	45.000004	38
CACCGGG	30	2.1615706E-6	45.000004	3
TGTCGGA	25	3.8857546E-5	45.000004	20
TCAACGG	25	3.8857546E-5	45.000004	2
AACCGCC	20	7.027193E-4	45.0	33
ATGTGCG	20	7.027193E-4	45.0	12
AGTATGC	20	7.027193E-4	45.0	36
TAGCACG	20	7.027193E-4	45.0	1
GTAGCTA	20	7.027193E-4	45.0	16
TACGACG	20	7.027193E-4	45.0	1
TACTCAC	45	3.8380676E-10	45.0	30
TTAGTTA	20	7.027193E-4	45.0	13
AATTACG	20	7.027193E-4	45.0	2
>>END_MODULE