FastQCFastQC Report
Sat 18 Jun 2016
SRR3551801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences340232
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151944.465776293823039No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC132973.908215570551859No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG115433.392684991417621No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC98192.885971925039385No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC41551.2212255167062476No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT36581.0751487220484846No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT25410.7468433304333513No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC24930.7327353100237485No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTA22490.6610195396082673No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT10740.315666956664864No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC8340.24512685461684966No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT6730.19780620282630676No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC5790.17017799619083449No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG5760.16929624491523432No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGT5420.15930306379176562No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTAT5110.15019163394389712No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC4450.13079310588069318No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTACGCAT4390.12902960332949281No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC3510.1031648992452209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT207.0262764E-445.0000044
TAGTTAA207.0262764E-445.00000438
ACCCACG207.0262764E-445.00000420
GTGCTAG253.884997E-545.01
GACCTAT253.884997E-545.033
GGATACC302.1609812E-644.9999968
CGTTTTT72800.044.1037061
GTACGGG850.042.352943
TTACGGG556.002665E-1140.9090923
GAATGAC3050.040.5737721
GCGCGAC501.0768417E-940.59
CGCGACC501.0768417E-940.510
AAACACG501.0768417E-940.540
TAAGCGG451.9213985E-840.02
ATGGGAC850.039.7058835
TATGGGA850.039.7058834
GTTTTTT82150.039.631772
CCGGGAT403.4486584E-739.3750045
GGTTATG403.4486584E-739.3750041
AATGACT3100.039.1935462
Produced by FastQC (version 0.11.5)