##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	340232
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.545313198053094	28.0	25.0	31.0	16.0	33.0
2	27.44563709468833	30.0	25.0	31.0	16.0	33.0
3	27.624664934515273	30.0	25.0	33.0	16.0	33.0
4	30.215517646782196	32.0	28.0	35.0	19.0	37.0
5	31.643560864351382	33.0	30.0	35.0	26.0	35.0
6	32.15685767358743	35.0	32.0	35.0	26.0	36.0
7	33.16540478261892	35.0	32.0	35.0	28.0	37.0
8	33.516006724823065	35.0	33.0	35.0	28.0	37.0
9	34.69027016859084	37.0	33.0	39.0	28.0	39.0
10	35.379223588610124	37.0	34.0	39.0	30.0	39.0
11	35.70586540948529	37.0	35.0	39.0	31.0	39.0
12	34.744738884055586	37.0	33.0	39.0	29.0	39.0
13	35.32610101342613	37.0	34.0	39.0	30.0	39.0
14	36.524321639351975	38.0	35.0	40.0	31.0	41.0
15	36.47548731453831	38.0	35.0	40.0	31.0	41.0
16	36.5771032707094	38.0	34.0	40.0	31.0	41.0
17	35.1778551106304	37.0	32.0	40.0	27.0	41.0
18	34.92291730348703	37.0	33.0	39.0	27.0	40.0
19	34.477712266923746	37.0	32.0	38.0	27.0	40.0
20	34.64018963530767	35.0	34.0	39.0	27.0	40.0
21	35.375499659056175	36.0	34.0	39.0	30.0	40.0
22	35.9633779303534	37.0	35.0	39.0	31.0	40.0
23	36.01172141362365	37.0	35.0	40.0	31.0	40.0
24	35.99172035552211	37.0	35.0	40.0	31.0	41.0
25	35.317653836205885	36.0	34.0	39.0	30.0	40.0
26	35.3762902960333	37.0	34.0	39.0	30.0	40.0
27	35.918673140680475	37.0	35.0	40.0	31.0	41.0
28	35.814144466128994	37.0	35.0	40.0	31.0	41.0
29	35.89836053046157	37.0	35.0	40.0	31.0	41.0
30	35.61189717604458	37.0	35.0	40.0	30.0	41.0
31	35.17581532601284	37.0	35.0	40.0	27.0	41.0
32	34.831653107291494	37.0	34.0	40.0	25.0	41.0
33	34.28958181476169	37.0	34.0	40.0	21.0	41.0
34	33.60976333795763	37.0	33.0	40.0	17.0	41.0
35	33.383141503444705	37.0	33.0	40.0	15.0	41.0
36	32.94828234851513	37.0	33.0	40.0	11.0	41.0
37	32.901246796303695	37.0	33.0	40.0	10.0	41.0
38	32.66006724823062	37.0	32.0	40.0	10.0	41.0
39	32.286924804251214	36.0	31.0	40.0	10.0	40.0
40	32.19946389522443	36.0	31.0	39.0	10.0	40.0
41	31.747325354464014	36.0	30.0	39.0	10.0	40.0
42	32.06156387406241	36.0	31.0	40.0	10.0	40.0
43	32.021644054645066	36.0	31.0	40.0	10.0	40.0
44	31.90078534646947	36.0	31.0	40.0	10.0	40.0
45	31.93326318512074	36.0	31.0	40.0	10.0	40.0
46	31.597357097509935	35.0	30.0	39.0	10.0	40.0
47	31.582602459498226	35.0	30.0	39.0	10.0	40.0
48	31.690002704037244	36.0	30.0	39.0	10.0	40.0
49	31.61408391920807	35.0	30.0	39.0	10.0	40.0
50	31.555500364457195	35.0	30.0	39.0	10.0	40.0
51	28.649974135295917	33.0	23.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	13.0
11	17.0
12	14.0
13	8.0
14	29.0
15	41.0
16	99.0
17	219.0
18	407.0
19	713.0
20	1127.0
21	1787.0
22	2415.0
23	3608.0
24	5601.0
25	8784.0
26	12165.0
27	13202.0
28	12338.0
29	11629.0
30	12106.0
31	14141.0
32	17975.0
33	23324.0
34	30803.0
35	46851.0
36	53415.0
37	46472.0
38	19365.0
39	1556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08099179383479	11.09948505725505	14.766394695384328	32.05312845352583
2	39.6253144912883	31.64840461802535	16.707717087164053	12.018563803522303
3	21.095311434550542	30.39102729901949	37.41182487243998	11.101836393989984
4	19.331514966258318	15.562028263067553	51.6456417973618	13.460814973312326
5	26.438430247595758	18.13350889980954	38.0158244962261	17.412236356368595
6	16.66127818664911	31.615779820828138	40.253415316607494	11.469526675915258
7	50.862058830445115	4.319993416257143	39.955089468362765	4.862858284934986
8	46.36571515906793	13.360001410802042	32.49753109642832	7.77675233370171
9	42.46249617907781	5.698170660020222	32.42493357473724	19.414399586164734
10	28.10523407557196	16.96665804509864	39.34756283947424	15.580545039855156
11	22.792094805897154	16.66921394812951	42.62356274542077	17.915128500552562
12	19.30006583742858	13.37175809447671	47.89849279315291	19.4296832749418
13	17.685873168896517	16.114004561593266	52.966799125302735	13.233323144207482
14	16.22863222742129	22.872628089068638	41.713889346093254	19.184850337416822
15	12.369206894119307	17.36168261656752	55.01775259234874	15.251357896964423
16	13.780302852171458	20.86194126360836	40.79422276564227	24.56353311857791
17	14.014848691481108	18.7548496320158	46.28753321263138	20.94276846387171
18	14.713489618848316	19.246572926708836	45.843718403912625	20.196219050530225
19	14.273789649415694	21.89741117825484	41.800594888193935	22.02820428413553
20	15.354228879117779	21.11441604552188	48.067495120976275	15.463859954384068
21	15.575254532201557	25.559618142914246	43.02093865362458	15.84418867125961
22	13.512250464389005	17.881034117896025	43.1020597709798	25.50465564673517
23	14.969491405864233	24.361612076465473	43.82774107079875	16.841155446871547
24	19.16339438971055	17.681464412518515	39.47130193515013	23.6838392626208
25	13.282407298549225	27.707858167368148	38.52341931387994	20.486315220202687
26	14.415751604787323	17.4298713818806	42.37520280279339	25.77917421053869
27	18.488560746784547	20.29909003268358	37.70515413012298	23.507195090408896
28	11.753450586658515	18.542641491688023	46.08473041924334	23.61917750241012
29	17.324061228808578	17.344929342331113	37.154647417056594	28.17636201180371
30	15.158774013026404	19.747407651249734	40.90385384090855	24.189964494815303
31	19.000270403724517	17.986844270968046	40.04708551811705	22.965799807190386
32	17.699687272214256	25.355345764066872	34.397999012438575	22.5469679512803
33	15.348644454372309	18.94795316137224	33.96447130193515	31.7389310823203
34	22.509934397705095	18.516188953420016	34.75892920125091	24.214947447623974
35	14.734063815278986	21.47593406851795	30.03773895459569	33.752263161607374
36	16.275658962119966	24.47124315173176	34.47089045122152	24.782207434926757
37	16.687436807825247	24.304298243551457	35.251534247219546	23.756730701403747
38	16.15368336899527	22.934644595452514	32.7361917750241	28.17548026052811
39	24.423040748665617	23.97187801265019	31.819464365491783	19.78561687319241
40	18.4200780643796	19.42938935784994	33.99739001622422	28.15314256154624
41	19.496696371887417	27.41776199769569	29.780267582120434	23.305274048296457
42	17.771403042629736	20.71204354675633	37.257812316301816	24.258741094312118
43	20.738790002116204	23.274118836558582	30.745491311810763	25.24159984951445
44	19.718309859154928	19.534317759646356	34.19343271649933	26.55393966469938
45	18.011239389592983	17.536269369136352	34.15845658256719	30.294034658703474
46	24.776329093089423	21.801888123398154	32.429342331115244	20.992440452397187
47	15.03444708316678	18.2690046791601	44.63924616144278	22.057302076230336
48	18.81333913329728	20.856944673046627	33.94830586188248	26.381410331773615
49	17.078640457099862	17.001928096122647	42.90425356815349	23.015177878623998
50	19.318582614216183	16.00496132051071	38.54840226668861	26.128053798584496
51	18.035046674034188	16.370006348609184	33.68701356721296	31.90793341014367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	392.0
2	616.0
3	12522.5
4	24429.0
5	16026.5
6	7624.0
7	7039.0
8	6454.0
9	6207.0
10	5960.0
11	5655.0
12	5350.0
13	5022.0
14	4694.0
15	4338.0
16	3982.0
17	3730.0
18	3478.0
19	3164.0
20	2850.0
21	2611.5
22	2373.0
23	2299.5
24	2226.0
25	2239.5
26	2420.0
27	2587.0
28	2578.5
29	2570.0
30	2867.0
31	3164.0
32	3433.5
33	3703.0
34	3946.0
35	4189.0
36	4616.0
37	5043.0
38	5407.5
39	5772.0
40	6176.0
41	6580.0
42	7452.0
43	8324.0
44	9973.5
45	11623.0
46	13719.5
47	15816.0
48	42441.5
49	69067.0
50	49979.0
51	30891.0
52	29510.0
53	28129.0
54	22522.0
55	16915.0
56	14520.5
57	12126.0
58	10613.5
59	9101.0
60	8587.0
61	8073.0
62	7394.0
63	6715.0
64	5971.0
65	5227.0
66	4379.0
67	3531.0
68	2991.0
69	2451.0
70	2167.0
71	1883.0
72	1603.0
73	1323.0
74	1225.5
75	937.0
76	746.0
77	610.5
78	475.0
79	377.5
80	280.0
81	221.0
82	162.0
83	127.0
84	92.0
85	67.0
86	42.0
87	35.0
88	28.0
89	17.5
90	7.0
91	8.0
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	340232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.99163690993326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.8628520658473	38.32748998935858
2	9.112965850038828	8.746922964953697
3	3.9962575555374866	5.753608248117976
4	2.4001668058636483	4.6075173548113
5	1.5479271604514415	3.71437791237048
6	0.9108292047082406	2.62273106875927
7	0.6022726955503871	2.0232836767934668
8	0.395965285493988	1.520241776829243
9	0.2527748152123348	1.0917969436481263
>10	0.8236523459803895	6.827131033265857
>50	0.0608220335589513	1.9979907715504859
>100	0.023584053828981117	1.9953101041467955
>500	0.003723797972997019	1.1065199338565974
>1k	0.003723797972997019	4.816265768630196
>5k	6.206329954995031E-4	2.9246081374260915
>10k+	0.0018618989864985095	11.924204315481836
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15194	4.465776293823039	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTC	13297	3.908215570551859	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCG	11543	3.392684991417621	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	9819	2.885971925039385	No Hit
GCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	4155	1.2212255167062476	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCT	3658	1.0751487220484846	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCT	2541	0.7468433304333513	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTC	2493	0.7327353100237485	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTACGCATCGTA	2249	0.6610195396082673	No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGCT	1074	0.315666956664864	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCC	834	0.24512685461684966	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTT	673	0.19780620282630676	No Hit
CCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	579	0.17017799619083449	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATG	576	0.16929624491523432	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTACGCATCGT	542	0.15930306379176562	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCTACGCATCGTAT	511	0.15019163394389712	No Hit
TCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC	445	0.13079310588069318	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTACGCAT	439	0.12902960332949281	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGC	351	0.1031648992452209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9391709186672623E-4	0.0	0.0	0.3697477015683416	0.0
2	2.9391709186672623E-4	0.0	0.0	2.1414799313409674	0.0
3	2.9391709186672623E-4	0.0	0.0	2.560605704342919	0.0
4	2.9391709186672623E-4	0.0	0.0	4.328517011921277	0.0
5	2.9391709186672623E-4	0.0	0.0	9.939100378565215	0.0
6	2.9391709186672623E-4	0.0	0.0	11.060100166944908	0.0
7	2.9391709186672623E-4	0.0	0.0	12.758940957934586	0.0
8	2.9391709186672623E-4	0.0	0.0	14.897775635448752	0.0
9	2.9391709186672623E-4	0.0	0.0	15.527639963319148	0.0
10	2.9391709186672623E-4	0.0	0.0	20.38608949187613	0.0
11	2.9391709186672623E-4	0.0	0.0	21.97529920759952	0.0
12	2.9391709186672623E-4	0.0	0.0	26.686496273131276	0.0
13	2.9391709186672623E-4	0.0	0.0	27.53268358061558	0.0
14	2.9391709186672623E-4	0.0	0.0	27.950339768158198	0.0
15	2.9391709186672623E-4	0.0	0.0	29.09132591878483	0.0
16	2.9391709186672623E-4	0.0	0.0	30.072127254344096	0.0
17	2.9391709186672623E-4	0.0	0.0	30.912436219991065	0.0
18	2.9391709186672623E-4	0.0	0.0	31.928801523666205	0.0
19	2.9391709186672623E-4	0.0	0.0	32.65154365256648	0.0
20	2.9391709186672623E-4	0.0	0.0	33.281114063345015	0.0
21	2.9391709186672623E-4	0.0	0.0	33.92244115779821	0.0
22	2.9391709186672623E-4	0.0	0.0	34.548190646382466	0.0
23	2.9391709186672623E-4	0.0	0.0	35.06636647934351	0.0
24	2.9391709186672623E-4	0.0	0.0	35.47814432504879	0.0
25	2.9391709186672623E-4	0.0	0.0	35.8428954360554	0.0
26	2.9391709186672623E-4	0.0	0.0	36.207646547062005	0.0
27	2.9391709186672623E-4	0.0	0.0	36.58973876648875	0.0
28	2.9391709186672623E-4	0.0	0.0	36.935973100707756	0.0
29	2.9391709186672623E-4	0.0	0.0	37.29572762115262	0.0
30	2.9391709186672623E-4	0.0	0.0	37.70750546685791	0.0
31	2.9391709186672623E-4	0.0	0.0	38.097239530673185	0.0
32	2.9391709186672623E-4	0.0	0.0	38.444061699075924	0.0
33	2.9391709186672623E-4	0.0	0.0	38.79529262385666	0.0
34	2.9391709186672623E-4	0.0	0.0	39.123891932563666	0.0
35	5.878341837334525E-4	0.0	0.0	39.456606080556796	0.0
36	5.878341837334525E-4	0.0	0.0	39.77873921324273	0.0
37	5.878341837334525E-4	0.0	0.0	40.09293658444826	0.0
38	5.878341837334525E-4	0.0	0.0	40.4274142349926	0.0
39	5.878341837334525E-4	0.0	0.0	40.7824660819676	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	20	7.0262764E-4	45.000004	4
TAGTTAA	20	7.0262764E-4	45.000004	38
ACCCACG	20	7.0262764E-4	45.000004	20
GTGCTAG	25	3.884997E-5	45.0	1
GACCTAT	25	3.884997E-5	45.0	33
GGATACC	30	2.1609812E-6	44.999996	8
CGTTTTT	7280	0.0	44.103706	1
GTACGGG	85	0.0	42.35294	3
TTACGGG	55	6.002665E-11	40.909092	3
GAATGAC	305	0.0	40.573772	1
GCGCGAC	50	1.0768417E-9	40.5	9
CGCGACC	50	1.0768417E-9	40.5	10
AAACACG	50	1.0768417E-9	40.5	40
TAAGCGG	45	1.9213985E-8	40.0	2
ATGGGAC	85	0.0	39.705883	5
TATGGGA	85	0.0	39.705883	4
GTTTTTT	8215	0.0	39.63177	2
CCGGGAT	40	3.4486584E-7	39.375004	5
GGTTATG	40	3.4486584E-7	39.375004	1
AATGACT	310	0.0	39.193546	2
>>END_MODULE