FastQCFastQC Report
Sat 18 Jun 2016
SRR3551800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences407835
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC124183.0448588277121877No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG120622.9575686245663073No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109932.6954528179288193No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC96542.367133767332377No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC41051.0065345053759487Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT39690.9731876861966237No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT24560.6022043228266333No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA24180.5928868292324102No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC22010.539679036865399No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT21830.5352654872681354No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC14060.3447472629862567Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT13060.3202275430014589Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC10150.24887515784569741No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT8740.21430235266713255No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC6630.16256574349920924Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG6440.15790699670209765No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT6080.14907989750757047No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT5620.13780082631456347No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG4590.11254551473022177No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTATGC4220.1034732183358466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG207.027928E-445.02
AAAACGC207.027928E-445.020
CTACGGC207.027928E-445.06
AACGCTC207.027928E-445.022
TTGCCCG253.886364E-545.01
GTAAGTC207.027928E-445.09
GTTATCA207.027928E-445.038
TAGTCGG302.1620453E-644.9999962
CGTTTTT53250.043.9436651
CGGGTAT556.002665E-1140.9090926
TACGGCT11800.038.8983047
CACGACC1050.038.5714327
TCAAGCG1050.038.5714317
GTTTTTT61400.038.5138442
ACAACGA1700.038.3823513
ACACAAC1700.038.3823511
AACGAGC1700.038.3823515
ACGGCTG12100.038.119838
TCGCAGG301.1386309E-437.4999962
CGTTAGG301.1386309E-437.4999962
Produced by FastQC (version 0.11.5)