##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	407835
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.952137506589676	28.0	16.0	31.0	16.0	33.0
2	25.81440533549107	28.0	16.0	31.0	16.0	33.0
3	26.361879191339636	30.0	22.0	33.0	16.0	33.0
4	29.25375948606667	32.0	19.0	35.0	19.0	37.0
5	31.33294837372957	33.0	28.0	35.0	19.0	37.0
6	30.33799943604644	35.0	28.0	35.0	17.0	37.0
7	32.32939056235978	33.0	32.0	35.0	28.0	37.0
8	32.85398016354653	35.0	32.0	35.0	28.0	37.0
9	34.23065455392499	35.0	32.0	38.0	28.0	39.0
10	33.97828778795346	35.0	32.0	39.0	27.0	39.0
11	34.39929628403644	37.0	32.0	39.0	27.0	39.0
12	34.51058638910344	37.0	33.0	39.0	27.0	39.0
13	34.747145291600766	37.0	34.0	39.0	27.0	39.0
14	35.03663246165729	37.0	33.0	39.0	27.0	41.0
15	35.49493054789314	37.0	33.0	40.0	27.0	41.0
16	35.055343459977685	37.0	32.0	40.0	27.0	41.0
17	34.17229516838918	36.0	32.0	39.0	25.0	41.0
18	34.26947172263293	37.0	32.0	39.0	26.0	40.0
19	33.73237216030993	37.0	32.0	39.0	25.0	40.0
20	34.1714541419937	35.0	32.0	38.0	27.0	40.0
21	34.99146958941729	36.0	34.0	39.0	29.0	40.0
22	35.865828092243184	37.0	34.0	39.0	30.0	40.0
23	36.366493802640775	38.0	35.0	40.0	31.0	40.0
24	35.5524194833695	37.0	34.0	39.0	30.0	40.0
25	34.362291122635376	36.0	33.0	39.0	25.0	40.0
26	34.96296296296296	37.0	33.0	39.0	27.0	40.0
27	35.59520149079898	37.0	34.0	39.0	30.0	40.0
28	35.73560385940392	37.0	35.0	40.0	30.0	40.0
29	35.89475645788125	38.0	35.0	40.0	30.0	41.0
30	34.47810511603957	36.0	33.0	39.0	25.0	40.0
31	34.82062353647921	36.0	33.0	39.0	27.0	40.0
32	34.86601934605907	37.0	34.0	39.0	27.0	40.0
33	34.75814728995795	37.0	34.0	40.0	25.0	40.0
34	34.814932509470744	38.0	34.0	40.0	24.0	41.0
35	34.662780291049074	38.0	34.0	40.0	23.0	41.0
36	34.45158213493202	38.0	34.0	40.0	21.0	41.0
37	34.51835423639462	38.0	34.0	40.0	21.0	41.0
38	34.15716650115856	37.0	33.0	40.0	21.0	41.0
39	34.20650998565596	37.0	34.0	40.0	21.0	41.0
40	33.77583091201098	37.0	33.0	40.0	19.0	41.0
41	33.467740630402	37.0	33.0	40.0	18.0	40.0
42	34.04669290276705	37.0	34.0	40.0	20.0	40.0
43	33.95998136501281	37.0	33.0	40.0	19.0	40.0
44	34.13811222675837	38.0	34.0	40.0	20.0	40.0
45	34.12028884230142	37.0	34.0	40.0	20.0	40.0
46	33.949072541591576	37.0	33.0	40.0	20.0	40.0
47	33.939463263329536	37.0	33.0	40.0	20.0	41.0
48	33.79785207252933	37.0	33.0	40.0	20.0	40.0
49	33.8837152279721	37.0	34.0	40.0	20.0	40.0
50	33.89890519450268	37.0	33.0	40.0	20.0	40.0
51	32.593416455184084	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	10.0
11	17.0
12	18.0
13	24.0
14	31.0
15	53.0
16	82.0
17	186.0
18	359.0
19	631.0
20	989.0
21	1476.0
22	2199.0
23	3268.0
24	4988.0
25	7651.0
26	10213.0
27	11161.0
28	11200.0
29	12171.0
30	15030.0
31	20107.0
32	26617.0
33	34542.0
34	47404.0
35	66944.0
36	69597.0
37	47186.0
38	13167.0
39	505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.243958953988745	12.843429328037073	17.005896992656343	26.906714725317837
2	35.2287076881582	29.588681697255016	18.862284992705384	16.3203256218814
3	24.313754336925474	28.00691456103571	32.751235180894234	14.92809592114458
4	22.38012921892432	18.133558914757195	43.623769416553266	15.862542449765224
5	28.36146971201589	20.586021307636667	32.42267093309795	18.6298380472495
6	20.369021785771206	30.636409332205428	34.11036325965158	14.884205622371793
7	58.16592494513713	4.394669412875305	32.07816886731153	5.361236774676033
8	55.284367452523696	11.665992374367084	24.582980862358554	8.466659310750671
9	49.86256696948521	5.457844471416137	25.41959370823985	19.259994850858803
10	31.95238270378952	18.466536712150745	32.45332058307894	17.12776000098079
11	25.218777201564357	18.876506430296565	36.58808096411539	19.316635404023685
12	21.0246790981647	15.097036791839837	41.986097318768614	21.892186791226845
13	20.19542216827884	17.926122083685804	46.192700479360525	15.68575526867483
14	17.641202937462456	23.553397820196896	37.658857135851505	21.146542106489143
15	13.962754545343092	19.62680986183138	48.22685644929935	18.18357914352618
16	15.934875623720377	21.456961761496686	36.22028516434342	26.387877450439518
17	16.148687581987815	21.18062451726801	39.92202729044834	22.748660610295833
18	17.406058822808244	20.172864025892824	39.55325070187698	22.867826449421948
19	16.64521191167997	23.303541873551804	35.90471636813908	24.146529846629154
20	18.076182769992766	22.191572572241224	41.8250027584685	17.90724189929751
21	18.57761104368188	26.018855664668312	36.80777765517918	18.59575563647063
22	16.071205266835854	19.361996885995563	38.01390268123138	26.55289516593721
23	16.687876224453515	25.241090146750523	37.851091740532326	20.219941888263637
24	20.502654259688356	19.6606470754104	34.65617222651317	25.180526438388075
25	16.584893400517366	27.04279917123347	33.426508269275566	22.945799158973603
26	18.664165655228217	19.390930155577625	37.099071928598576	24.845832260595586
27	20.83146370468449	22.504444199247246	33.213431902607674	23.45066019346059
28	16.664582490467957	20.713769048757463	39.97842264641338	22.6432258143612
29	24.275993968148885	19.819044466512192	33.94755231895252	21.957409246386405
30	18.733311265585346	27.025144972844412	36.13716331359496	18.104380447975284
31	21.054593156546154	19.823703213309305	37.494575011953366	21.62712861819118
32	22.68343815513627	24.502310983608567	34.872926551178786	17.94132431007638
33	16.825431853568233	24.52977306999154	32.58891463459487	26.055880441845353
34	24.572927777164786	20.03408241077887	37.41047237240551	17.982517439650838
35	21.783074037294494	24.294383758137485	31.188348229063223	22.734193975504798
36	22.36321061213481	22.450991209680385	33.47554770924516	21.710250468939645
37	19.15897360452144	23.929530324763693	38.27111454387191	18.640381526842965
38	20.217980310664853	25.644929934900144	31.943555604594998	22.19353414984001
39	22.25605943580124	20.225091029460444	37.25697892530067	20.26187060943764
40	24.50647933600598	23.21085733200927	33.511101303223114	18.771562028761632
41	19.713119276177864	23.390341682297986	32.44719065308274	24.449348388441404
42	24.00848382311474	19.97327350521657	35.42805301163461	20.590189660034085
43	23.365576765113342	20.197138548677774	30.10702857773364	26.330256108475243
44	21.485404636679046	20.109848345531894	33.82617970502777	24.578567312761287
45	20.805963195900304	18.160775803940318	33.703335907903934	27.329925092255447
46	25.710642784459402	21.635220125786166	32.06321183812081	20.590925251633628
47	17.44259320558559	19.87862738607525	41.27183787561146	21.406941532727696
48	21.032770605759683	20.935917711819734	32.81498645285471	25.21632522956588
49	19.773682984540315	17.34353353684701	39.611607635440805	23.27117584317187
50	20.43301825493153	16.98481003346942	36.22396312234114	26.35820858925791
51	20.038005565976434	16.955141172287814	32.571505633405664	30.435347628330085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	102.0
1	364.5
2	627.0
3	9551.0
4	18475.0
5	12148.0
6	5821.0
7	5367.5
8	4914.0
9	4741.5
10	4569.0
11	4397.0
12	4225.0
13	3855.0
14	3485.0
15	3256.0
16	3027.0
17	2744.0
18	2461.0
19	2341.5
20	2222.0
21	2120.5
22	2019.0
23	1960.5
24	1902.0
25	1952.5
26	2192.0
27	2381.0
28	2655.0
29	2929.0
30	3138.0
31	3347.0
32	3755.0
33	4163.0
34	4771.5
35	5380.0
36	5876.5
37	6373.0
38	7073.5
39	7774.0
40	9872.5
41	11971.0
42	14886.5
43	17802.0
44	45838.5
45	73875.0
46	57501.0
47	41127.0
48	40108.5
49	39090.0
50	32389.5
51	25689.0
52	22508.5
53	19328.0
54	17533.0
55	15738.0
56	14588.5
57	13439.0
58	12531.0
59	11623.0
60	11548.0
61	11473.0
62	10820.0
63	10167.0
64	9173.5
65	8180.0
66	6949.5
67	5719.0
68	4938.5
69	4158.0
70	3785.5
71	3413.0
72	2951.0
73	2489.0
74	2104.0
75	1466.5
76	1214.0
77	958.5
78	703.0
79	549.5
80	396.0
81	287.5
82	179.0
83	126.5
84	74.0
85	60.5
86	47.0
87	29.0
88	11.0
89	8.5
90	6.0
91	4.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	407835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.995245855104706
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86393343135164	36.59234694851877
2	9.245288710701539	8.689692319216856
3	4.219685152764066	5.9491542355584714
4	2.600075687578373	4.8876478471850415
5	1.8131051492947314	4.26036611261311
6	1.2697212337333716	3.5802516928048
7	0.884442762486569	2.9095223547476996
8	0.5766805997629233	2.1680997252582253
9	0.3912780465078775	1.6549387194008516
>10	1.053146058770555	8.061654631162565
>50	0.04714898632884986	1.5166877326808073
>100	0.02595843067543406	2.417438917322814
>500	0.002648819456676945	0.8342778385117147
>1k	0.004767875022018501	5.242929573625245
>5k	5.29763891335389E-4	2.403496942104474
>10k+	0.001589291674006167	8.831494409288586
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	12418	3.0448588277121877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	12062	2.9575686245663073	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10993	2.6954528179288193	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	9654	2.367133767332377	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4105	1.0065345053759487	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	3969	0.9731876861966237	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	2456	0.6022043228266333	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	2418	0.5928868292324102	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	2201	0.539679036865399	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	2183	0.5352654872681354	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1406	0.3447472629862567	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1306	0.3202275430014589	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	1015	0.24887515784569741	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	874	0.21430235266713255	No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	663	0.16256574349920924	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG	644	0.15790699670209765	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	608	0.14907989750757047	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	562	0.13780082631456347	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTG	459	0.11254551473022177	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTATGC	422	0.1034732183358466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3346941777924896	0.0
2	0.0	0.0	0.0	2.049358196329398	0.0
3	0.0	0.0	0.0	2.5816813171993576	0.0
4	0.0	0.0	0.0	4.33582208491179	0.0
5	0.0	0.0	0.0	9.060036534382778	0.0
6	0.0	0.0	0.0	10.495420942292839	0.0
7	0.0	0.0	0.0	12.293942403177756	0.0
8	0.0	0.0	0.0	14.398469969472949	0.0
9	0.0	0.0	0.0	15.28461264972354	0.0
10	0.0	0.0	0.0	19.767798251743965	0.0
11	0.0	0.0	0.0	21.509679159464	0.0
12	0.0	0.0	0.0	25.60913114372234	0.0
13	0.0	0.0	0.0	26.50017776796989	0.0
14	0.0	0.0	0.0	26.948398249291994	0.0
15	0.0	0.0	0.0	28.027756323022793	0.0
16	0.0	0.0	0.0	28.925423271666237	0.0
17	0.0	0.0	0.0	29.97658366741452	0.0
18	0.0	0.0	0.0	31.13881839469393	0.0
19	0.0	0.0	0.0	32.342736645947504	0.0
20	0.0	0.0	0.0	33.09892481027867	0.0
21	0.0	0.0	0.0	33.77836625105741	0.0
22	0.0	0.0	0.0	34.49924601861047	0.0
23	0.0	0.0	0.0	35.09286843944242	0.0
24	0.0	0.0	0.0	35.54942562555936	0.0
25	0.0	0.0	0.0	35.948116272512166	0.0
26	0.0	0.0	0.0	36.38971642943837	0.0
27	0.0	0.0	0.0	36.87814925153555	0.0
28	0.0	0.0	0.0	37.27953706768669	0.0
29	0.0	0.0	0.0	37.69122316623144	0.0
30	0.0	0.0	0.0	38.15685264874275	0.0
31	0.0	0.0	0.0	38.57221670528523	0.0
32	0.0	0.0	0.0	39.02019198940748	0.0
33	0.0	0.0	0.0	39.413243100763786	0.0
34	0.0	0.0	0.0	39.80212585972268	0.0
35	0.0	0.0	0.0	40.20155209827504	0.0
36	0.0	0.0	0.0	40.55439086885628	0.0
37	2.4519719984797774E-4	0.0	0.0	40.92022509102946	0.0
38	2.4519719984797774E-4	0.0	0.0	41.307146272389566	0.0
39	2.4519719984797774E-4	0.0	0.0	41.72765947012885	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.027928E-4	45.0	2
AAAACGC	20	7.027928E-4	45.0	20
CTACGGC	20	7.027928E-4	45.0	6
AACGCTC	20	7.027928E-4	45.0	22
TTGCCCG	25	3.886364E-5	45.0	1
GTAAGTC	20	7.027928E-4	45.0	9
GTTATCA	20	7.027928E-4	45.0	38
TAGTCGG	30	2.1620453E-6	44.999996	2
CGTTTTT	5325	0.0	43.943665	1
CGGGTAT	55	6.002665E-11	40.909092	6
TACGGCT	1180	0.0	38.898304	7
CACGACC	105	0.0	38.57143	27
TCAAGCG	105	0.0	38.57143	17
GTTTTTT	6140	0.0	38.513844	2
ACAACGA	170	0.0	38.38235	13
ACACAAC	170	0.0	38.38235	11
AACGAGC	170	0.0	38.38235	15
ACGGCTG	1210	0.0	38.11983	8
TCGCAGG	30	1.1386309E-4	37.499996	2
CGTTAGG	30	1.1386309E-4	37.499996	2
>>END_MODULE