FastQCFastQC Report
Sat 18 Jun 2016
SRR3551799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460613
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC183263.978611111714172No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG160933.493822362807823No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143263.110203142334237No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC118072.563323223617223No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT49071.0653194764368352No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC45980.9982349608022353Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT37220.8080536155080296No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC33780.7333705301413551No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA21640.46980871143454483No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC15240.33086343633375526Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT14010.3041598912753222Illumina Single End Adapter 2 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT12160.26399602269150024No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC10330.22426635809236822No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC6670.14480702889410416Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT5960.1293927874376103No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT5550.12049160575146597No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG5340.11593246391222133No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT5150.11180752605766663No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG207.0288783E-445.0000041
TAGCCGT207.0288783E-445.00000444
TCGTCCC207.0288783E-445.00000438
GTTCACG302.1626583E-645.0000041
CGTAAGG650.045.0000042
TGTAGCG207.0288783E-445.0000041
CATATGG253.8871534E-545.02
CTTTAGG351.2098099E-745.02
CGTTTTT68150.044.0425531
GCATAGG650.041.5384642
TAGGGTG603.6379788E-1241.2500045
TACGGCT15350.041.1889277
TTCACGG451.9239451E-840.02
ACGGCTG15950.039.6394968
ACAACGA1650.039.54545213
GTGTTAT403.4522054E-739.3750049
GTTTTTT78150.039.06912
GCGAGAC1050.038.5714321
ACGGGCG700.038.5714265
CGTTAGG356.2401414E-638.5714262
Produced by FastQC (version 0.11.5)