##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551799_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460613
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.39693842770395	28.0	25.0	31.0	16.0	33.0
2	27.31701450024207	30.0	25.0	31.0	16.0	33.0
3	27.586857079587418	30.0	25.0	33.0	16.0	33.0
4	30.717209457831196	33.0	28.0	35.0	19.0	37.0
5	31.714843046114634	33.0	30.0	35.0	26.0	35.0
6	32.146550357892636	35.0	32.0	35.0	25.0	37.0
7	33.149908925714215	35.0	32.0	35.0	29.0	37.0
8	33.611956240922424	35.0	33.0	35.0	28.0	37.0
9	34.63391393642819	37.0	33.0	39.0	28.0	39.0
10	35.237170900517356	37.0	34.0	39.0	30.0	39.0
11	35.566451663326916	37.0	34.0	39.0	30.0	39.0
12	34.51783384316118	35.0	33.0	39.0	27.0	39.0
13	35.14124438519972	37.0	34.0	39.0	30.0	39.0
14	36.42019222210402	38.0	35.0	40.0	31.0	41.0
15	36.34962973255205	38.0	35.0	40.0	31.0	41.0
16	36.506383884084904	38.0	34.0	40.0	31.0	41.0
17	35.02052482235629	37.0	32.0	40.0	27.0	41.0
18	34.8846993028855	37.0	32.0	39.0	27.0	40.0
19	34.54281142738047	37.0	32.0	39.0	27.0	40.0
20	34.794085273320555	36.0	33.0	39.0	27.0	40.0
21	35.69863204034623	37.0	34.0	39.0	30.0	40.0
22	36.301463484530395	38.0	35.0	39.0	32.0	40.0
23	36.36115784834558	38.0	35.0	40.0	31.0	41.0
24	36.45028690028288	38.0	35.0	40.0	32.0	41.0
25	35.797854163907665	37.0	35.0	40.0	30.0	41.0
26	35.88191605534364	38.0	35.0	40.0	30.0	41.0
27	36.36307485893798	38.0	35.0	40.0	31.0	41.0
28	36.3529665901744	38.0	35.0	40.0	31.0	41.0
29	36.44806377588127	38.0	35.0	40.0	31.0	41.0
30	36.07316337142026	38.0	35.0	40.0	31.0	41.0
31	35.915371472364	38.0	35.0	40.0	30.0	41.0
32	35.672577630244916	38.0	35.0	40.0	30.0	41.0
33	35.296101065319476	38.0	35.0	40.0	26.0	41.0
34	34.84762479565275	38.0	35.0	40.0	23.0	41.0
35	34.55672766508978	38.0	34.0	40.0	21.0	41.0
36	34.3335989214373	38.0	34.0	40.0	18.0	41.0
37	34.20914954636539	38.0	34.0	40.0	18.0	41.0
38	34.1022745775738	38.0	34.0	40.0	18.0	41.0
39	33.8049772802765	37.0	34.0	40.0	18.0	40.0
40	33.58255194707922	37.0	33.0	40.0	16.0	41.0
41	33.44049777144805	37.0	33.0	40.0	15.0	41.0
42	33.631721206305514	38.0	33.0	40.0	15.0	41.0
43	33.56664271308018	38.0	33.0	40.0	15.0	41.0
44	33.33284774854379	37.0	33.0	40.0	15.0	40.0
45	33.34691378662782	37.0	33.0	40.0	15.0	40.0
46	33.0179608478267	37.0	33.0	40.0	15.0	40.0
47	33.03618221804422	37.0	32.0	39.0	15.0	40.0
48	33.05509397259738	37.0	33.0	40.0	15.0	40.0
49	32.97776658496395	36.0	33.0	40.0	15.0	40.0
50	32.8906609235953	36.0	32.0	39.0	15.0	40.0
51	29.950759097116233	34.0	26.0	37.0	10.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	9.0
10	13.0
11	11.0
12	17.0
13	15.0
14	33.0
15	67.0
16	141.0
17	305.0
18	525.0
19	805.0
20	1324.0
21	1874.0
22	2614.0
23	3886.0
24	5787.0
25	8707.0
26	12238.0
27	13325.0
28	13000.0
29	12524.0
30	13799.0
31	17335.0
32	23111.0
33	31058.0
34	42150.0
35	63972.0
36	80309.0
37	76101.0
38	33028.0
39	2526.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46816090731265	12.335952307034322	16.364713978980188	27.83117280667285
2	35.09909620440587	32.38705811603233	18.286717917210325	14.227127762351474
3	23.057099995006656	30.80395038785271	33.48950203316016	12.649447583980479
4	21.399309181460357	17.273285816943943	46.40728767967904	14.920117321916662
5	27.847455456098718	19.917588083705844	33.40222703223748	18.83272942795796
6	18.960602501449156	31.957413273181608	36.3096569137215	12.772327311647738
7	56.79974295124106	4.511813604913453	33.247216209703154	5.441227234142327
8	52.59599707346514	11.969049939971299	27.298621619450604	8.136331367112955
9	47.73638607681503	5.597323566638371	27.19376135714797	19.472528999398627
10	29.95790392368431	18.178601125022524	34.747390976807	17.116103974486172
11	23.407936814636148	18.347072271082233	38.31546222099681	19.92952869328482
12	20.798153764657098	14.661548849033787	43.95533777813479	20.584959608174323
13	18.31515827820752	17.459776428368283	49.116720544144435	15.108344749279764
14	17.001691224520368	23.66238034966447	38.443335294487994	20.892593131327168
15	13.133150822925103	19.010318857696156	51.40041640162132	16.456113917757424
16	15.141127150123857	22.32763730072751	36.77989982914073	25.7513357200079
17	15.09987777157831	20.235208298506553	41.65253694533155	23.01237698458359
18	16.58659221515676	20.98290756014268	40.848174063693385	21.582326161007177
19	15.894905267545639	23.43333774774051	36.75059106017416	23.92116592453969
20	16.889232392485663	22.811774743656823	42.83726251755812	17.461730346299387
21	17.319962745298113	26.616270057510317	38.44116427456455	17.62260292262702
22	15.206040645835007	18.974714130951583	37.956375525658196	27.862869697555215
23	15.766597990069755	25.693369488051793	39.5080034649478	19.032029056930654
24	20.73063504503781	18.65405448825804	35.0760833932173	25.539227073486853
25	15.523877962628063	28.729540850996386	34.15383412973581	21.592747056639737
26	18.156890925788026	18.642982286648444	37.91490904512031	25.285217742443223
27	21.17677963930675	22.459852414065605	33.15212553705605	23.211242409571593
28	15.887306697813566	19.40414187180996	41.45844776417513	23.250103666201344
29	25.123259656153863	19.17336245394724	34.08978904199404	21.613588847904857
30	17.990590799651766	27.815541463224008	36.955752442940174	17.238115294184055
31	20.556519247177132	19.177487391801794	39.17670582462935	21.089287536391723
32	22.598580587174048	23.720563683612923	36.62315219066765	17.057703538545375
33	15.824347120033522	25.16689715661521	33.31212970541431	25.696626017936968
34	24.46652612931029	18.94453695401563	39.22837609880746	17.360560817866624
35	22.1461400351271	24.428099076665227	31.187135404341603	22.238625483866066
36	21.653535614496334	22.09772629083417	34.51161821312034	21.73711988154915
37	18.50555672549407	23.41162754850601	40.01884445293554	18.06397127306437
38	19.197677877089877	25.64408733578948	32.291967443385225	22.866267343735412
39	22.368018271303676	19.347912455792606	38.502604138398176	19.781465134505538
40	24.378382720418227	22.81568257951903	34.83054103987512	17.97539366018762
41	18.65839652810494	23.922251434501415	32.53707559274272	24.882276444650934
42	23.727510947367964	19.264545290732134	37.32308901398788	19.684854747912023
43	23.183019150566743	19.944291628764276	30.223853864306914	26.648835356362067
44	21.36240184276171	19.504225890280996	34.29560173073708	24.837770536220212
45	20.240852950307524	17.55508420300773	34.063953904904984	28.140108941779758
46	26.503811225475616	21.713238662391205	31.64695742412828	20.135992688004897
47	16.63630857140376	18.605640743965107	43.38913578209907	21.36891490253206
48	21.188937350878067	20.400857118665776	32.71575053244264	25.694454998013516
49	18.72070479990795	16.61025633232236	41.389409330609425	23.27962953716026
50	19.865483605543048	16.461758569558395	37.57905226296261	26.09370556193594
51	19.848115446155447	16.374700670628055	32.435688962317606	31.34149492089889
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	484.0
2	812.0
3	11853.0
4	22894.0
5	15146.0
6	7398.0
7	6830.5
8	6263.0
9	5994.0
10	5725.0
11	5419.0
12	5113.0
13	4840.5
14	4568.0
15	4223.5
16	3879.0
17	3618.5
18	3358.0
19	3143.5
20	2929.0
21	2773.0
22	2617.0
23	2534.5
24	2452.0
25	2393.5
26	2553.5
27	2772.0
28	3104.0
29	3436.0
30	3676.5
31	3917.0
32	4385.5
33	4854.0
34	5549.0
35	6244.0
36	6668.5
37	7093.0
38	7986.0
39	8879.0
40	11246.5
41	13614.0
42	17076.0
43	20538.0
44	57177.0
45	93816.0
46	68586.5
47	43357.0
48	42302.5
49	41248.0
50	33858.0
51	26468.0
52	23264.0
53	20060.0
54	18138.0
55	16216.0
56	15088.5
57	13961.0
58	13220.0
59	12479.0
60	12329.0
61	12179.0
62	11336.0
63	10493.0
64	9390.0
65	8287.0
66	6993.0
67	5699.0
68	4998.5
69	4298.0
70	3872.5
71	3447.0
72	2921.0
73	2395.0
74	2028.0
75	1414.5
76	1168.0
77	934.0
78	700.0
79	545.0
80	390.0
81	300.0
82	210.0
83	146.5
84	83.0
85	73.0
86	63.0
87	55.5
88	48.0
89	35.5
90	23.0
91	17.0
92	11.0
93	7.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460613.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.87711712893083
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.91255119337936	34.96490685719104
2	9.00720143559034	8.084344676577231
3	3.998276631052247	5.382933860567959
4	2.5268093149321698	4.535836703547378
5	1.8006805106243715	4.040467509353645
6	1.3032819312459323	3.5092521528325724
7	0.9424849905186444	2.9607206518235123
8	0.6599396604368232	2.369295155156012
9	0.4744488790090851	1.916270812348258
>10	1.2783304031303662	8.98237329740742
>50	0.06005844158893483	1.8170843384129016
>100	0.027075526945821677	2.136316784492149
>500	0.0024614115405292435	0.6333836836751799
>1k	0.004430540772952638	5.289538032171202
>5k	0.0	0.0
>10k+	0.001969129232423395	13.377275484443496
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTC	18326	3.978611111714172	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCG	16093	3.493822362807823	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14326	3.110203142334237	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGC	11807	2.563323223617223	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCT	4907	1.0653194764368352	No Hit
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	4598	0.9982349608022353	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCT	3722	0.8080536155080296	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTC	3378	0.7333705301413551	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTATGCTCGTA	2164	0.46980871143454483	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1524	0.33086343633375526	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1401	0.3041598912753222	Illumina Single End Adapter 2 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTT	1216	0.26399602269150024	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCC	1033	0.22426635809236822	No Hit
TCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	667	0.14480702889410416	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTATGCTCGTAT	596	0.1293927874376103	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTATGCTCGT	555	0.12049160575146597	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATG	534	0.11593246391222133	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTATGCT	515	0.11180752605766663	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1710199234498375E-4	0.0	0.0	0.3432382498974193	0.0
2	2.1710199234498375E-4	0.0	0.0	2.0657254571625203	0.0
3	2.1710199234498375E-4	0.0	0.0	2.623460475496784	0.0
4	2.1710199234498375E-4	0.0	0.0	4.407821750580205	0.0
5	2.1710199234498375E-4	0.0	0.0	10.28976602918285	0.0
6	2.1710199234498375E-4	0.0	0.0	11.730454850384161	0.0
7	2.1710199234498375E-4	0.0	0.0	13.713030244478553	0.0
8	2.1710199234498375E-4	0.0	0.0	16.018870505174625	0.0
9	2.1710199234498375E-4	0.0	0.0	16.828226732636725	0.0
10	2.1710199234498375E-4	0.0	0.0	21.929038042782118	0.0
11	2.1710199234498375E-4	0.0	0.0	23.638282028514176	0.0
12	2.1710199234498375E-4	0.0	0.0	27.940809312807065	0.0
13	2.1710199234498375E-4	0.0	0.0	28.784250553067327	0.0
14	2.1710199234498375E-4	0.0	0.0	29.20868494810177	0.0
15	2.1710199234498375E-4	0.0	0.0	30.137230169361263	0.0
16	2.1710199234498375E-4	0.0	0.0	31.02126948219004	0.0
17	2.1710199234498375E-4	0.0	0.0	31.987373348125217	0.0
18	2.1710199234498375E-4	0.0	0.0	33.14148753943115	0.0
19	2.1710199234498375E-4	0.0	0.0	33.87681198750361	0.0
20	2.1710199234498375E-4	0.0	0.0	34.511835315112684	0.0
21	4.342039846899675E-4	0.0	0.0	35.13274701321934	0.0
22	4.342039846899675E-4	0.0	0.0	35.78535560220836	0.0
23	4.342039846899675E-4	0.0	0.0	36.34222221257325	0.0
24	4.342039846899675E-4	0.0	0.0	36.8115967200231	0.0
25	4.342039846899675E-4	0.0	0.0	37.222570791532156	0.0
26	4.342039846899675E-4	0.0	0.0	37.59945985024304	0.0
27	4.342039846899675E-4	0.0	0.0	38.013256247652585	0.0
28	4.342039846899675E-4	0.0	0.0	38.39926359004197	0.0
29	4.342039846899675E-4	0.0	0.0	38.80546141771943	0.0
30	4.342039846899675E-4	0.0	0.0	39.301539470227716	0.0
31	4.342039846899675E-4	0.0	0.0	39.710559623805665	0.0
32	4.342039846899675E-4	0.0	0.0	40.12978357102383	0.0
33	4.342039846899675E-4	0.0	0.0	40.51969874927542	0.0
34	4.342039846899675E-4	0.0	0.0	40.89550229802459	0.0
35	4.342039846899675E-4	0.0	0.0	41.26761511290389	0.0
36	4.342039846899675E-4	0.0	0.0	41.60868234287786	0.0
37	4.342039846899675E-4	0.0	0.0	41.95886785653032	0.0
38	4.342039846899675E-4	0.0	0.0	42.30644814627464	0.0
39	4.342039846899675E-4	0.0	0.0	42.68203459303146	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTAG	20	7.0288783E-4	45.000004	1
TAGCCGT	20	7.0288783E-4	45.000004	44
TCGTCCC	20	7.0288783E-4	45.000004	38
GTTCACG	30	2.1626583E-6	45.000004	1
CGTAAGG	65	0.0	45.000004	2
TGTAGCG	20	7.0288783E-4	45.000004	1
CATATGG	25	3.8871534E-5	45.0	2
CTTTAGG	35	1.2098099E-7	45.0	2
CGTTTTT	6815	0.0	44.042553	1
GCATAGG	65	0.0	41.538464	2
TAGGGTG	60	3.6379788E-12	41.250004	5
TACGGCT	1535	0.0	41.188927	7
TTCACGG	45	1.9239451E-8	40.0	2
ACGGCTG	1595	0.0	39.639496	8
ACAACGA	165	0.0	39.545452	13
GTGTTAT	40	3.4522054E-7	39.375004	9
GTTTTTT	7815	0.0	39.0691	2
GCGAGAC	105	0.0	38.57143	21
ACGGGCG	70	0.0	38.571426	5
CGTTAGG	35	6.2401414E-6	38.571426	2
>>END_MODULE