##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551791_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 282665 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.390918578529355 28.0 25.0 31.0 16.0 33.0 2 27.363217943502026 30.0 25.0 31.0 16.0 33.0 3 27.560946703695187 30.0 25.0 31.0 16.0 33.0 4 30.482624307926343 33.0 28.0 35.0 19.0 37.0 5 31.57662604142713 33.0 30.0 35.0 26.0 35.0 6 32.11649125289654 35.0 32.0 35.0 26.0 36.0 7 33.050101710505366 35.0 32.0 35.0 28.0 37.0 8 33.26316664602975 35.0 33.0 35.0 28.0 37.0 9 34.50103479383723 37.0 33.0 39.0 28.0 39.0 10 35.19430774945607 37.0 34.0 39.0 30.0 39.0 11 35.50540038561548 37.0 34.0 39.0 30.0 39.0 12 34.40948826349212 35.0 32.0 39.0 27.0 39.0 13 34.944305096138535 37.0 33.0 39.0 30.0 39.0 14 36.19744573965649 38.0 34.0 40.0 31.0 41.0 15 36.008593211044875 38.0 34.0 40.0 30.0 41.0 16 36.27881414395132 38.0 34.0 40.0 31.0 41.0 17 34.91232023773725 37.0 32.0 40.0 27.0 41.0 18 34.70585675623087 37.0 32.0 39.0 27.0 40.0 19 34.29439088673872 37.0 32.0 38.0 27.0 40.0 20 34.47616790193339 35.0 33.0 39.0 27.0 40.0 21 35.39831249005006 36.0 34.0 39.0 30.0 40.0 22 35.976070613623904 37.0 35.0 39.0 31.0 40.0 23 36.01895176268728 37.0 35.0 40.0 31.0 40.0 24 36.0980984557692 37.0 35.0 40.0 31.0 41.0 25 35.40997647391789 37.0 34.0 39.0 30.0 40.0 26 35.558756832292644 37.0 34.0 39.0 30.0 40.0 27 36.08265260998001 38.0 35.0 40.0 31.0 41.0 28 36.01832911750659 38.0 35.0 40.0 31.0 41.0 29 36.09963384218067 38.0 35.0 40.0 31.0 41.0 30 35.777407885659706 38.0 35.0 40.0 30.0 41.0 31 35.54343834574496 38.0 35.0 40.0 30.0 41.0 32 35.19030300886208 38.0 35.0 40.0 27.0 41.0 33 34.4651477897865 37.0 34.0 40.0 22.0 41.0 34 34.02425839774999 37.0 33.0 40.0 20.0 41.0 35 33.77992323067943 38.0 33.0 40.0 17.0 41.0 36 33.110363858277466 37.0 33.0 40.0 15.0 41.0 37 33.31403604974086 37.0 33.0 40.0 15.0 41.0 38 32.890991102541875 37.0 32.0 40.0 12.0 41.0 39 32.64486229282012 36.0 32.0 39.0 12.0 40.0 40 32.28879061786921 36.0 31.0 39.0 10.0 40.0 41 31.803155678983956 35.0 30.0 39.0 10.0 40.0 42 32.25313003024782 36.0 31.0 39.0 10.0 40.0 43 31.940243751437215 36.0 31.0 39.0 10.0 40.0 44 32.007390373764 36.0 31.0 39.0 10.0 40.0 45 32.098247041550955 36.0 31.0 39.0 10.0 40.0 46 31.701622061450834 35.0 30.0 39.0 10.0 40.0 47 31.66268197336069 35.0 30.0 39.0 10.0 40.0 48 31.713830859851768 35.0 31.0 39.0 10.0 40.0 49 31.644030919993632 35.0 30.0 39.0 10.0 40.0 50 31.532467762191995 35.0 30.0 39.0 10.0 40.0 51 28.353658924875738 32.0 23.0 36.0 8.0 39.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 6.0 10 5.0 11 6.0 12 6.0 13 20.0 14 22.0 15 54.0 16 135.0 17 238.0 18 419.0 19 601.0 20 927.0 21 1382.0 22 2012.0 23 2947.0 24 4584.0 25 6847.0 26 9327.0 27 9875.0 28 9732.0 29 9314.0 30 10191.0 31 12543.0 32 16444.0 33 20966.0 34 27010.0 35 39392.0 36 44789.0 37 36985.0 38 14752.0 39 1129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.74968602409212 10.440273822369235 14.331806201687511 28.478233951851127 2 35.15221198238197 35.818017794916244 16.76436771443228 12.265402508269506 3 19.758371216811422 34.77437956591725 34.48959015088533 10.977659066386005 4 19.035607521270762 15.247023862168998 52.756443139405306 12.960925477154936 5 29.922700015919908 17.673217412838518 34.597491730493694 17.806590840747884 6 16.78488670334141 34.18711195231104 37.50482019351529 11.523181150832256 7 51.394406806643914 3.8848106415721793 39.72759273344772 4.993189818336194 8 45.80935029098049 15.866838837493146 30.09640387030584 8.227407001220525 9 42.13149841685387 5.223851555728513 29.04781278191499 23.596837245502627 10 30.122583269948528 17.19455893018237 36.5595316010118 16.123326198857306 11 25.09189323050254 16.479932075071197 39.70176710947588 18.726407584950383 12 21.246705464065236 13.18486547680116 44.83788229883431 20.730546760299294 13 18.661666637185363 16.00728777881945 52.31882263456742 13.012222949427768 14 17.4613057860011 23.637521447650045 38.5590716926397 20.342101073709163 15 11.848300992340757 17.314488882599544 55.75256929580953 15.084640829250173 16 14.465533405267719 21.715458227937663 36.698565439654715 27.120442927139898 17 14.87379052942529 18.71331788512904 44.84389648523871 21.56899510020696 18 15.837475456812836 19.97063661931969 43.3608688730476 20.831019050819872 19 14.824261935506694 22.63209099110254 38.85836591017636 23.685281163214405 20 15.403746484354272 22.235508464082926 47.2368351228486 15.123909928714202 21 16.018962375957404 27.62457325809704 40.61380078891975 15.742663577025809 22 13.511754196663894 17.36649390621407 41.79965683759928 27.322095059522756 23 14.278032299718749 26.664072311747123 42.124069127766084 16.933826260768047 24 20.572409035430635 17.283710399235844 35.88063608865618 26.26324447667734 25 13.89277059416624 31.002423363345304 34.95622026073267 20.148585781755788 26 16.757645976686184 16.543965471494527 40.087382590699235 26.611005961120053 27 19.93101374418481 21.011090867281055 34.206215838536785 24.851679549997346 28 14.001025949445456 17.754232041462508 45.25250738506713 22.992234624024906 29 27.939433605150978 15.880636088656184 33.13215290184494 23.0477774043479 30 16.74561760387738 22.631737215431695 38.53501494702209 22.087630233668833 31 24.73953266233881 15.828631065041657 37.69515150443104 21.736684768188493 32 30.645463711460565 21.883501671590043 31.02966409000053 16.441370526948862 33 18.164611819645163 21.8336193020006 33.20113915766012 26.800629720694108 34 34.5292130260202 16.633470716218845 33.12543116409884 15.71188509366211 35 24.780570640157077 19.023932924132808 29.22399306599685 26.97150336971326 36 25.110997116728285 19.59987971627191 39.19834432986043 16.090778837139368 37 25.994021191162688 17.984893778854826 34.98699874409637 21.03408628588612 38 20.492455733819185 25.335290892045354 32.725310880370756 21.446942493764702 39 34.36046203102613 17.2338280296464 31.664691419171103 16.74101852015637 40 27.125395786531758 22.16015424619249 33.037341022057916 17.67710894521784 41 24.32384624909345 22.230201829020217 33.22554967894858 20.220402242937755 42 26.227159358250933 16.59950825181752 38.85836591017636 18.31496647975519 43 24.894132630499 17.525339182424425 34.20267808182832 23.377850105248264 44 23.625493074841245 17.19137494914475 34.278032299718745 24.90509967629526 45 21.45507933419419 15.399501176304106 33.304441653547485 29.84097783595422 46 29.18366263952028 20.99021810270108 31.51433675906108 18.311782498717562 47 17.01059558134187 16.616135708347336 46.35664125378098 20.016627456529815 48 22.385155572851257 20.13018944687174 31.904905099676295 25.579749880600712 49 19.59634195956344 14.951974952682503 43.53174252206676 21.9199405656873 50 21.87501105548971 15.214122724780216 37.47262660746821 25.438239612261864 51 20.760617692321297 15.15008932835689 31.761272177312367 32.32802080200944 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 95.0 1 240.5 2 386.0 3 8347.5 4 16309.0 5 10562.0 6 4815.0 7 4447.5 8 4080.0 9 4032.5 10 3985.0 11 3895.0 12 3805.0 13 3579.0 14 3353.0 15 3179.0 16 3005.0 17 2718.0 18 2431.0 19 2292.5 20 2154.0 21 1958.0 22 1762.0 23 1681.5 24 1601.0 25 1612.0 26 1663.0 27 1703.0 28 1812.0 29 1921.0 30 2094.0 31 2267.0 32 2482.0 33 2697.0 34 2958.5 35 3220.0 36 3540.0 37 3860.0 38 4121.0 39 4382.0 40 4675.5 41 4969.0 42 5876.0 43 6783.0 44 7769.0 45 8755.0 46 11016.0 47 13277.0 48 39745.5 49 66214.0 50 46958.0 51 27702.0 52 26354.0 53 25006.0 54 20147.0 55 15288.0 56 13079.5 57 10871.0 58 9528.5 59 8186.0 60 7423.0 61 6660.0 62 6169.5 63 5679.0 64 4951.5 65 4224.0 66 3604.5 67 2985.0 68 2512.5 69 2040.0 70 1812.0 71 1584.0 72 1365.0 73 1146.0 74 927.5 75 588.0 76 467.0 77 378.0 78 289.0 79 236.0 80 183.0 81 139.0 82 95.0 83 68.5 84 42.0 85 32.0 86 22.0 87 16.5 88 11.0 89 13.0 90 15.0 91 10.0 92 5.0 93 3.0 94 1.0 95 1.0 96 1.0 97 1.5 98 2.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 282665.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.31037703287299 #Duplication Level Percentage of deduplicated Percentage of total 1 80.79577710818285 38.224786776521 2 8.508566674310822 8.050869947419665 3 3.8056338944110575 5.401379231810037 4 2.2579639746979683 4.273005078784214 5 1.526170133189592 3.6101842208754786 6 0.9752336865797048 2.7683204044346708 7 0.6320852339171658 2.0932933513472984 8 0.3718474648926537 1.4073795010231558 9 0.25263507487199505 1.0757034579529963 >10 0.7901265488014468 6.459777429402562 >50 0.04797726065414436 1.5608574844873935 >100 0.024738275024793185 2.180448037861508 >500 0.004497868186326034 1.5385139213147723 >1k 0.0037482234886050286 4.92374107183333 >5k 0.0 0.0 >10k+ 0.0029985787908840223 16.43174008493193 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTC 12907 4.566182583623724 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCG 12470 4.4115826154635345 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGC 10590 3.746484354270957 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10364 3.6665310526595087 No Hit GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 3887 1.3751260325827392 No Hit GAACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCT 3008 1.0641572179081245 No Hit GAATGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCT 2648 0.9367979764031629 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTC 2292 0.8108538375815896 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTA 2048 0.7245325738948932 No Hit CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT 968 0.34245484938000814 Illumina Single End Adapter 1 (95% over 21bp) GAATGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTT 835 0.29540268515734175 No Hit CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 752 0.2660393044770311 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCC 641 0.22677020501300124 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATG 584 0.20660499177471564 No Hit GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTAT 558 0.19740682433269063 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGAGGTT 489 0.17299630304423963 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGT 476 0.16839721932322715 No Hit TCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC 416 0.1471706790724002 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGAGGGTCGTATGC 306 0.10825535527921744 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.415332637574514 0.0 2 0.0 0.0 0.0 2.5974209753595243 0.0 3 0.0 0.0 0.0 3.0619284311817876 0.0 4 0.0 0.0 0.0 4.853802204022429 0.0 5 0.0 0.0 0.0 11.528487785894963 0.0 6 0.0 0.0 0.0 12.684980453894186 0.0 7 0.0 0.0 0.0 14.597845506164541 0.0 8 0.0 0.0 0.0 16.739957193143827 0.0 9 0.0 0.0 0.0 17.358357065784585 0.0 10 0.0 0.0 0.0 23.511223533157626 0.0 11 0.0 0.0 0.0 25.020076769320575 0.0 12 0.0 0.0 0.0 30.780252242053315 0.0 13 0.0 0.0 0.0 31.60631843348133 0.0 14 0.0 0.0 0.0 32.10655723205915 0.0 15 0.0 0.0 0.0 33.3451258556949 0.0 16 0.0 0.0 0.0 34.27626342136451 0.0 17 0.0 0.0 0.0 35.16353280384908 0.0 18 0.0 0.0 0.0 36.23335043249076 0.0 19 0.0 0.0 0.0 37.02403905683406 0.0 20 0.0 0.0 0.0 37.68878354235579 0.0 21 0.0 0.0 0.0 38.293032388162665 0.0 22 0.0 0.0 0.0 38.923814409283075 0.0 23 0.0 0.0 0.0 39.42299188084836 0.0 24 0.0 0.0 0.0 39.80542338103409 0.0 25 0.0 0.0 0.0 40.176180284081866 0.0 26 0.0 0.0 0.0 40.53278616029576 0.0 27 0.0 0.0 0.0 40.929368687315375 0.0 28 0.0 0.0 0.0 41.2697008826703 0.0 29 0.0 0.0 0.0 41.615339713087934 0.0 30 0.0 0.0 0.0 42.06781879610139 0.0 31 0.0 0.0 0.0 42.397537721330906 0.0 32 0.0 0.0 0.0 42.73150195461058 0.0 33 3.5377567084711583E-4 0.0 0.0 43.058390674473316 0.0 34 3.5377567084711583E-4 0.0 0.0 43.366883059452 0.0 35 3.5377567084711583E-4 0.0 0.0 43.70190861974422 0.0 36 7.075513416942317E-4 0.0 0.0 43.997311304901565 0.0 37 7.075513416942317E-4 0.0 0.0 44.296959298109066 0.0 38 7.075513416942317E-4 0.0 0.0 44.65604160401889 0.0 39 7.075513416942317E-4 0.0 0.0 45.121610386853696 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGG 25 3.8833175E-5 45.000004 2 GGGTAAC 25 3.8833175E-5 45.000004 7 GTACGGG 65 0.0 45.000004 3 TGATCCG 20 7.0242514E-4 45.0 4 CAGAATA 20 7.0242514E-4 45.0 9 CGGGTAT 20 7.0242514E-4 45.0 6 CGATTGG 35 1.2075907E-7 45.0 10 GCTTGTC 20 7.0242514E-4 45.0 38 ACGGGTA 20 7.0242514E-4 45.0 5 ATATCTC 20 7.0242514E-4 45.0 13 GTAGCAT 20 7.0242514E-4 45.0 29 CTACGGG 45 3.8198777E-10 45.0 3 GTACTAG 20 7.0242514E-4 45.0 1 GTCAAGC 45 3.8198777E-10 45.0 16 AGACACG 40 6.7830115E-9 45.0 24 ACTAAGG 20 7.0242514E-4 45.0 2 GCCAGTA 20 7.0242514E-4 45.0 25 CTCTAGG 20 7.0242514E-4 45.0 2 GGTTACG 20 7.0242514E-4 45.0 1 GACTAAG 20 7.0242514E-4 45.0 1 >>END_MODULE