FastQCFastQC Report
Sat 18 Jun 2016
SRR3551790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512136
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158003.085118015527126No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC136372.6627692644141403No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG93871.8329115703641221No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC88161.7214177484105784No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT48740.9517003295999501No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC36750.7175828295608979No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT17960.35068809847384286No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT16380.31983691831857164No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA16180.3159317056406892No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC15870.3098786259899714No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT12950.2528625208928878No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT5920.11559429526532014Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC5430.10602652420450819No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATACGTTCGT5220.10192605089273163No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCAGG351.2101555E-745.02
ATCCGTT207.0296193E-445.028
TTAAGCG207.0296193E-445.01
CTTGACG253.887766E-545.01
CGGTATG207.0296193E-445.01
TGTAACG253.887766E-545.01
TAGCGAC302.163135E-644.99999630
GTAGCGA302.163135E-644.99999629
TCGGTAG302.163135E-644.99999626
CGTTTTT81400.044.1707571
CGAGACA1150.041.0869622
GTGCAAG556.002665E-1140.9090921
ACGACCA1100.040.90909228
CCCTCGT800.039.37514
ACGGGTA403.453224E-739.3755
CGTGTAC800.039.37518
GCGAGAC1200.039.37499621
ACACGAC1150.039.13043626
CACGACC1150.039.13043627
AACACGT1150.039.13043641
Produced by FastQC (version 0.11.5)