##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551790_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512136
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.869528797038285	28.0	16.0	31.0	16.0	33.0
2	25.933709014792946	28.0	16.0	31.0	16.0	33.0
3	26.586840214318073	30.0	23.0	33.0	16.0	33.0
4	28.941900198384804	32.0	19.0	35.0	19.0	37.0
5	31.237522845494166	33.0	28.0	35.0	19.0	37.0
6	30.041223425027727	33.0	28.0	35.0	17.0	37.0
7	32.28749199431401	33.0	32.0	35.0	28.0	37.0
8	33.05736562163175	35.0	33.0	35.0	28.0	37.0
9	34.296247481137826	37.0	32.0	38.0	28.0	39.0
10	34.00204242623053	35.0	32.0	39.0	27.0	39.0
11	34.35678999328304	37.0	32.0	39.0	27.0	39.0
12	34.64588703000766	37.0	33.0	39.0	27.0	39.0
13	34.88595412156146	37.0	34.0	39.0	29.0	39.0
14	35.16983769936111	37.0	33.0	40.0	27.0	41.0
15	35.72779496071356	38.0	34.0	40.0	27.0	41.0
16	35.522710764328224	37.0	33.0	40.0	27.0	41.0
17	34.53646101816705	37.0	32.0	40.0	25.0	41.0
18	34.628053876314105	37.0	32.0	39.0	27.0	40.0
19	34.12338714716403	37.0	32.0	39.0	25.0	40.0
20	34.41856264742178	35.0	33.0	38.0	27.0	40.0
21	35.03341885749098	36.0	34.0	39.0	29.0	40.0
22	35.82273068091288	37.0	34.0	39.0	30.0	40.0
23	36.351408610212914	38.0	35.0	40.0	31.0	40.0
24	35.48788017245419	36.0	34.0	39.0	30.0	40.0
25	34.25730665292032	36.0	33.0	39.0	25.0	40.0
26	34.71803583423153	36.0	33.0	39.0	27.0	40.0
27	35.407836980801974	37.0	34.0	39.0	30.0	40.0
28	35.509122576815535	37.0	34.0	39.0	30.0	40.0
29	35.63794773263352	38.0	35.0	40.0	30.0	41.0
30	34.386963619038696	36.0	33.0	39.0	25.0	40.0
31	34.4891142196604	36.0	33.0	39.0	25.0	40.0
32	34.37623209459987	36.0	33.0	39.0	25.0	40.0
33	34.23511918709093	37.0	33.0	40.0	22.0	40.0
34	34.1854214505522	38.0	34.0	40.0	21.0	40.0
35	34.05560632332037	38.0	34.0	40.0	18.0	41.0
36	33.57014738272646	37.0	33.0	40.0	15.0	41.0
37	33.719078526016524	37.0	33.0	40.0	15.0	41.0
38	33.27712560726057	37.0	33.0	40.0	15.0	41.0
39	33.24925996219754	37.0	33.0	40.0	15.0	40.0
40	32.91164846837559	37.0	32.0	40.0	15.0	41.0
41	32.53839214583626	36.0	31.0	39.0	13.0	40.0
42	33.05639127106862	37.0	33.0	40.0	14.0	40.0
43	32.97914225908743	37.0	33.0	40.0	14.0	40.0
44	33.2390790727463	37.0	33.0	40.0	15.0	40.0
45	33.234051111423526	37.0	33.0	40.0	15.0	40.0
46	33.059946967211836	37.0	33.0	40.0	15.0	40.0
47	33.03601191870909	36.0	32.0	40.0	15.0	41.0
48	32.90856725557274	36.0	32.0	40.0	15.0	40.0
49	32.961849977349765	36.0	33.0	40.0	15.0	40.0
50	32.92463915834856	36.0	33.0	39.0	15.0	40.0
51	31.60869183185716	35.0	30.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	16.0
11	14.0
12	23.0
13	31.0
14	48.0
15	70.0
16	135.0
17	280.0
18	503.0
19	921.0
20	1441.0
21	2185.0
22	3210.0
23	4916.0
24	7771.0
25	11850.0
26	15787.0
27	17346.0
28	16515.0
29	16996.0
30	20153.0
31	25754.0
32	33282.0
33	42274.0
34	56826.0
35	79745.0
36	80320.0
37	55873.0
38	17011.0
39	825.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91315588046925	12.786252089288782	16.868761422747085	29.431830607494884
2	39.00331162035084	26.067880406766953	19.49775059749754	15.431057375384663
3	25.210686223971756	24.399573550775575	35.795960448006	14.593779777246668
4	22.362419357358203	19.310495649593076	42.80269303466267	15.524391958386053
5	25.592811284502552	22.07811987440836	34.885655372791604	17.443413468297482
6	21.012973116515926	29.190488464001753	35.74890263523752	14.047635784244811
7	60.94084383835544	4.6339253635753	30.284729056344407	4.140501741724854
8	59.11886686348938	9.258282956089788	25.075956386584814	6.546893793836012
9	52.98592561350891	5.89023228205008	26.15906712279551	14.964774981645501
10	31.1222019151163	20.041551462892667	33.033608260305854	15.802638361685178
11	24.89436399706328	19.750417857756535	37.276817095459016	18.07840104972117
12	20.876095412156147	16.883601230923038	42.69295655841417	19.547346798506645
13	19.758423543746193	18.578463533124015	45.98544136713685	15.677671555992939
14	17.976474998828436	22.797460049674307	39.79470296952372	19.431361981973538
15	16.072879078994642	20.13254291828733	46.84790758704719	16.946670415670837
16	17.70623428151897	21.17230579377353	38.70983488760798	22.41162503709952
17	17.842721464610964	20.805411062686474	40.64603933330209	20.705828139400474
18	18.4394379617914	20.831575987628288	39.84547073433619	20.88351531624412
19	18.08894512395145	22.841198431666587	37.847759188965426	21.222097255416532
20	19.356967680459878	22.004116094162487	41.58485246106503	17.054063764312605
21	19.412226439851914	24.286322383116985	38.42573066529203	17.87572051173907
22	17.75212053048409	19.33431744692816	39.50200727931643	23.411554743271317
23	18.607557367574238	23.401401190308825	39.39754284018308	18.59349860193386
24	20.46311917147008	20.560554227783246	37.38303888029742	21.593287720449254
25	17.86712904384773	25.438555383726197	35.94299170532827	20.751323867097803
26	18.92641798272334	19.956417826514834	38.322437789962045	22.794726400799785
27	21.801240295546496	20.838800631082368	35.82993579830357	21.53002327506756
28	18.247887279941267	21.686622303450644	39.428784541606134	20.636705875001955
29	24.65185028976678	19.354038770951465	35.714732024306045	20.27937891497571
30	22.82616336285674	23.056570910851807	36.284893075276884	17.832372651014573
31	22.05449333770717	23.001702672727557	34.130973022790826	20.812830966774452
32	22.49031507255885	24.19240201821391	35.76374244341347	17.553540465813768
33	19.47861505537592	24.520830404423823	32.69365949669619	23.30689504350407
34	24.035217207929144	22.78301076276614	32.25725197994283	20.924520049361888
35	24.39039630098255	22.164620335223457	32.169970476592155	21.275012887201836
36	22.668588031304186	25.725588515550555	33.26401580830092	18.341807644844337
37	22.4290032335161	23.582407797928674	35.42086477029539	18.56772419825984
38	21.010044207007514	28.349305653185873	30.208382148491808	20.432267991314806
39	22.58872643204149	22.344455379039942	33.841010981458055	21.225807207460516
40	23.249683677773092	24.80669197244482	33.54070012652889	18.402924223253198
41	19.73225861880438	25.16538575690832	33.384491619413595	21.717864004873704
42	22.731266694784196	22.98491025821266	34.741748285611635	19.542074761391508
43	23.186223971757503	21.785228923567175	33.66176171954325	21.366785385132076
44	21.796944561600824	22.26146960963494	32.620827280253685	23.32075854851055
45	20.87961010356624	21.204328537732163	32.473991283565304	25.442070075136293
46	24.233406751331678	22.883765249855507	32.60130121686427	20.281526781948543
47	19.275153474858243	21.596216629957667	38.64207944764672	20.486550447537372
48	20.437344767796052	22.73243825858756	33.04708124404455	23.783135729571832
49	19.321820766358936	20.76011059562304	38.42065388881078	21.49741474920724
50	20.153045284846215	20.056977052970304	36.544589718356065	23.24538794382742
51	19.94255432150835	19.96012777855882	33.54675320617961	26.55056469375322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	104.0
1	411.5
2	719.0
3	13860.5
4	27002.0
5	18239.5
6	9477.0
7	8892.0
8	8307.0
9	8033.0
10	7759.0
11	7358.0
12	6957.0
13	6550.0
14	6143.0
15	5570.5
16	4998.0
17	4518.5
18	4039.0
19	3724.0
20	3409.0
21	3358.5
22	3308.0
23	3214.0
24	3120.0
25	3397.0
26	3876.5
27	4079.0
28	4591.5
29	5104.0
30	5671.5
31	6239.0
32	6891.5
33	7544.0
34	8279.0
35	9014.0
36	9635.5
37	10257.0
38	10902.5
39	11548.0
40	12605.0
41	13662.0
42	15260.0
43	16858.0
44	19699.0
45	22540.0
46	47950.5
47	73361.0
48	57623.5
49	41886.0
50	40662.5
51	39439.0
52	34276.0
53	29113.0
54	26301.0
55	23489.0
56	21754.0
57	20019.0
58	19051.0
59	18083.0
60	17545.5
61	17008.0
62	15887.0
63	14766.0
64	13423.0
65	12080.0
66	10364.0
67	8648.0
68	7367.5
69	6087.0
70	5182.0
71	4277.0
72	3610.0
73	2943.0
74	2510.0
75	1716.0
76	1355.0
77	1106.5
78	858.0
79	644.5
80	431.0
81	328.5
82	226.0
83	151.0
84	76.0
85	54.5
86	33.0
87	22.0
88	11.0
89	7.0
90	3.0
91	4.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	512136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.1651755378998
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.63045494359662	36.42754138369553
2	9.804553617877103	9.444760921516025
3	4.799716531810118	6.935375678603818
4	3.141857931614018	6.053125551653278
5	2.0819405778037527	5.013851669469715
6	1.4605652490741863	4.220902896372871
7	0.9176100503473543	3.093779440522498
8	0.6515049893625083	2.5103881741170206
9	0.3924213135250429	1.7010937305672214
>10	1.0489677325654823	7.805757076733237
>50	0.039526771893458816	1.2664328757538625
>100	0.025115969631345915	2.481899065986997
>500	0.0012352116212137336	0.32860621273195795
>1k	0.0028821604494987116	3.268808813796538
>5k	8.23474414142489E-4	3.60990880528656
>10k+	8.23474414142489E-4	5.837767703192907
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15800	3.085118015527126	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	13637	2.6627692644141403	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	9387	1.8329115703641221	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	8816	1.7214177484105784	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	4874	0.9517003295999501	No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	3675	0.7175828295608979	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGATACGTT	1796	0.35068809847384286	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	1638	0.31983691831857164	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA	1618	0.3159317056406892	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC	1587	0.3098786259899714	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	1295	0.2528625208928878	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	592	0.11559429526532014	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCC	543	0.10602652420450819	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGATACGTTCGT	522	0.10192605089273163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12067107174656731	0.0
2	0.0	0.0	0.0	1.0505022103503756	0.0
3	0.0	0.0	0.0	1.3014121249043222	0.0
4	0.0	0.0	0.0	2.6969398753456115	0.0
5	0.0	0.0	0.0	6.267866348001312	0.0
6	0.0	0.0	0.0	7.316220691378852	0.0
7	0.0	0.0	0.0	8.337824327912898	0.0
8	0.0	0.0	0.0	9.637088585844385	0.0
9	0.0	0.0	0.0	10.349985160191824	0.0
10	0.0	0.0	0.0	13.20821031913398	0.0
11	0.0	0.0	0.0	14.195252823468767	0.0
12	0.0	0.0	0.0	16.947451458206412	0.0
13	0.0	0.0	0.0	17.529523407844792	0.0
14	0.0	0.0	0.0	17.829639002140055	0.0
15	1.9526063389412187E-4	0.0	0.0	18.439633222425293	0.0
16	1.9526063389412187E-4	0.0	0.0	18.935595232516363	0.0
17	1.9526063389412187E-4	0.0	0.0	19.476076667135292	0.0
18	1.9526063389412187E-4	0.0	0.0	20.106768514613307	0.0
19	1.9526063389412187E-4	0.0	0.0	20.826303950513147	0.0
20	1.9526063389412187E-4	0.0	0.0	21.243575925144885	0.0
21	1.9526063389412187E-4	0.0	0.0	21.620233687926646	0.0
22	1.9526063389412187E-4	0.0	0.0	22.045511348548043	0.0
23	1.9526063389412187E-4	0.0	0.0	22.4223643719637	0.0
24	1.9526063389412187E-4	0.0	0.0	22.73321930112314	0.0
25	1.9526063389412187E-4	0.0	0.0	23.01127044378837	0.0
26	1.9526063389412187E-4	0.0	0.0	23.26862395926082	0.0
27	1.9526063389412187E-4	0.0	0.0	23.566201165315462	0.0
28	1.9526063389412187E-4	0.0	0.0	23.840542355936705	0.0
29	1.9526063389412187E-4	0.0	0.0	24.123279753815392	0.0
30	1.9526063389412187E-4	0.0	0.0	24.447412406079636	0.0
31	1.9526063389412187E-4	0.0	0.0	24.76217254791696	0.0
32	1.9526063389412187E-4	0.0	0.0	25.07400378024587	0.0
33	1.9526063389412187E-4	0.0	0.0	25.39794117187622	0.0
34	1.9526063389412187E-4	0.0	0.0	25.69297998969024	0.0
35	1.9526063389412187E-4	0.0	0.0	26.00520174328694	5.857819016823657E-4
36	1.9526063389412187E-4	0.0	0.0	26.30414577377884	5.857819016823657E-4
37	1.9526063389412187E-4	0.0	0.0	26.599965634128434	5.857819016823657E-4
38	1.9526063389412187E-4	0.0	0.0	26.907891653779465	5.857819016823657E-4
39	1.9526063389412187E-4	0.0	0.0	27.246083071684083	5.857819016823657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAGG	35	1.2101555E-7	45.0	2
ATCCGTT	20	7.0296193E-4	45.0	28
TTAAGCG	20	7.0296193E-4	45.0	1
CTTGACG	25	3.887766E-5	45.0	1
CGGTATG	20	7.0296193E-4	45.0	1
TGTAACG	25	3.887766E-5	45.0	1
TAGCGAC	30	2.163135E-6	44.999996	30
GTAGCGA	30	2.163135E-6	44.999996	29
TCGGTAG	30	2.163135E-6	44.999996	26
CGTTTTT	8140	0.0	44.170757	1
CGAGACA	115	0.0	41.08696	22
GTGCAAG	55	6.002665E-11	40.909092	1
ACGACCA	110	0.0	40.909092	28
CCCTCGT	80	0.0	39.375	14
ACGGGTA	40	3.453224E-7	39.375	5
CGTGTAC	80	0.0	39.375	18
GCGAGAC	120	0.0	39.374996	21
ACACGAC	115	0.0	39.130436	26
CACGACC	115	0.0	39.130436	27
AACACGT	115	0.0	39.130436	41
>>END_MODULE