FastQCFastQC Report
Sat 18 Jun 2016
SRR3551789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566389
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194423.4326231618198797No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC181313.20115680212716No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG107331.894987367339408No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC90241.593251281363162No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT57031.0069051482285143No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC40340.7122313463008639No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC21880.386306937458178No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT20450.3610592719844488No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT18240.3220401526159583No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA12470.2201667052149671No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT6020.10628737493136343Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT207.030251E-445.00000444
GTACGAG207.030251E-445.0000041
TTGTGCG453.8380676E-1045.01
CATAGCG351.2104647E-745.01
CGTAAGG253.888291E-545.02
GCATCGT253.888291E-545.035
GCATATC351.2104647E-745.028
CGCATCG302.1635442E-644.99999621
CATCGCC302.1635442E-644.99999623
ATCGCCA302.1635442E-644.99999624
CGTTTTT103800.043.959541
TCGTTCG650.041.5384645
CTACGAA650.041.5384611
CGAATAT556.002665E-1140.90909214
TACGGGA1300.039.807694
TACGGCT12750.039.705887
CGGTCTA403.454079E-739.37500431
GAATCTG24550.039.317721
ACACGAC1150.039.13043226
AGACACG1150.039.13043224
Produced by FastQC (version 0.11.5)