##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	566389
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.35543769388177	28.0	25.0	31.0	16.0	33.0
2	27.423968332718324	30.0	25.0	31.0	16.0	33.0
3	27.732533647369564	31.0	25.0	33.0	16.0	33.0
4	30.538056000381363	33.0	28.0	35.0	19.0	37.0
5	31.757846638970744	33.0	30.0	35.0	26.0	35.0
6	32.04788934813352	35.0	32.0	35.0	25.0	37.0
7	33.227232520405586	35.0	32.0	35.0	29.0	37.0
8	33.82709939635127	35.0	35.0	36.0	30.0	37.0
9	34.93918314091552	37.0	34.0	39.0	28.0	39.0
10	35.38808839860943	37.0	34.0	39.0	30.0	39.0
11	35.64474062879046	37.0	35.0	39.0	31.0	39.0
12	34.70830648194086	37.0	33.0	39.0	28.0	39.0
13	35.33116109246472	37.0	34.0	39.0	30.0	39.0
14	36.62062469433552	38.0	35.0	40.0	32.0	41.0
15	36.71010559880224	38.0	35.0	40.0	31.0	41.0
16	36.86639394479766	38.0	35.0	40.0	32.0	41.0
17	35.45207445766072	38.0	33.0	40.0	27.0	41.0
18	35.27200563570267	37.0	33.0	39.0	27.0	40.0
19	34.88942934979316	37.0	33.0	39.0	27.0	40.0
20	35.0634934647389	36.0	34.0	39.0	29.0	40.0
21	35.75058661096879	37.0	35.0	39.0	31.0	40.0
22	36.32824436915265	38.0	35.0	39.0	32.0	40.0
23	36.408616692767694	38.0	35.0	40.0	32.0	41.0
24	36.428897806984246	38.0	35.0	40.0	32.0	41.0
25	35.73462408344795	37.0	35.0	40.0	30.0	41.0
26	35.76816110482372	37.0	35.0	40.0	30.0	41.0
27	36.28964545568505	38.0	35.0	40.0	31.0	41.0
28	36.19183458718302	38.0	35.0	40.0	31.0	41.0
29	36.27315502243158	38.0	35.0	40.0	31.0	41.0
30	35.93692497559098	38.0	35.0	40.0	30.0	41.0
31	35.59374387567555	38.0	35.0	40.0	30.0	41.0
32	35.258428394619244	38.0	35.0	40.0	27.0	41.0
33	34.77181760238987	38.0	34.0	40.0	23.0	41.0
34	34.205275879298505	38.0	34.0	40.0	19.0	41.0
35	33.977393628760446	38.0	34.0	40.0	17.0	41.0
36	33.39410546461884	37.0	33.0	40.0	15.0	41.0
37	33.35755461352533	37.0	33.0	40.0	14.0	41.0
38	33.05466207853613	37.0	33.0	40.0	10.0	41.0
39	32.64640203111289	37.0	32.0	40.0	10.0	40.0
40	32.515562625686584	37.0	32.0	40.0	10.0	41.0
41	32.2286290870762	37.0	31.0	40.0	10.0	41.0
42	32.376767557279535	37.0	31.0	40.0	10.0	41.0
43	32.242838402581974	36.0	31.0	40.0	10.0	41.0
44	32.134169272355216	36.0	31.0	40.0	10.0	40.0
45	32.198019382438574	36.0	31.0	40.0	10.0	40.0
46	31.831695177695895	36.0	31.0	39.0	10.0	40.0
47	31.84481160474515	36.0	31.0	39.0	10.0	40.0
48	31.883770694699226	36.0	31.0	39.0	10.0	40.0
49	31.77129499337028	35.0	31.0	39.0	10.0	40.0
50	31.691134538276696	35.0	30.0	39.0	10.0	40.0
51	28.891066034121426	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	14.0
11	15.0
12	17.0
13	29.0
14	57.0
15	75.0
16	217.0
17	442.0
18	729.0
19	1072.0
20	1697.0
21	2564.0
22	3692.0
23	5540.0
24	8495.0
25	13491.0
26	18623.0
27	21107.0
28	19595.0
29	18106.0
30	19080.0
31	22551.0
32	28595.0
33	37026.0
34	48845.0
35	71967.0
36	86876.0
37	85721.0
38	45756.0
39	4376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.740727662436946	12.432621396248868	16.45829986104956	30.36835108026462
2	39.12823165704136	28.283388272017994	19.06834348830927	13.520036582631374
3	24.187263523832563	26.561603420970393	36.692273331579536	12.558859723617513
4	21.812393955391084	18.332276933344396	45.02064835298708	14.834680758277438
5	24.48405601097479	21.47534644917186	36.102925727724234	17.937671812129118
6	19.901869563144764	30.158954358223767	37.919698299225445	12.019477779406026
7	59.91641786828487	4.710543460413249	31.244074302290475	4.1289643690114035
8	56.94319628382614	9.200567101409101	27.63930796678608	6.216928647978686
9	51.247287641532594	6.023598622148382	28.063398123904243	14.66571561241479
10	29.403289965024037	19.74491030016473	35.20778122456474	15.644018510246491
11	23.25062810188757	19.37555284442318	38.86498501912996	18.50883403455929
12	20.522997445218746	16.435170880790412	44.79995197646847	18.24187969752237
13	18.265361791983956	18.25106066678555	48.32050057469336	15.163076966537133
14	17.2176719533748	23.28629263633298	40.71071295523041	18.785322455061802
15	15.430914089080119	19.84201670583292	49.151907964314276	15.575161240772683
16	16.969962340370312	21.89308055064629	39.48381765888815	21.653139450095253
17	16.929177649989672	20.250746395145384	41.813841723621046	21.006234231243898
18	17.827323623869816	21.39571919652394	40.939530958404916	19.83742622120133
19	17.4773874492619	22.778337856137743	38.53888405318606	21.205390641414294
20	18.457985589409397	22.474483084946918	42.47699019578417	16.590541129859513
21	18.595700128357013	24.153541117500517	40.20364096054125	17.047117793601217
22	17.04535222258907	18.960290542365758	39.74618151129348	24.248175723751697
23	17.89335597972418	23.21955405207375	41.039638834793756	17.847451133408313
24	20.84203612711405	19.629618513071406	38.00832996403532	21.520015395779225
25	17.161879909390894	26.560720635464317	36.628712775142176	19.648686680002612
26	18.57204147679422	19.293100678155824	38.94231702946208	23.192540815587872
27	22.26031932117326	20.278112745833692	36.267653503157725	21.193914429835324
28	17.798015145068142	20.69072669137289	40.417098495910054	21.094159667648913
29	25.354835634166623	18.8386426996287	36.100277371206005	19.70624429499867
30	23.24074090421954	22.758386903700462	36.940865730090096	17.060006461989904
31	21.52954947924483	23.335905181774365	34.88680041455608	20.247744924424733
32	22.35071655699528	23.37333528723192	37.58494603532201	16.691002120450786
33	18.59305177183879	24.909558624902672	33.82569223625459	22.671697367003947
34	23.32831322642212	22.221476758905983	32.9506752426336	21.4995347720383
35	25.12654730229577	21.545969289657815	32.54971406577458	20.77776934227183
36	22.00766610933475	25.618788500482886	34.684465976563814	17.689079413618554
37	21.582516609609296	22.918877308704793	37.201464011483274	18.297142070202636
38	20.23097199980932	27.936100453928308	30.601936125171925	21.230991421090454
39	22.70630255884207	21.64484126633815	34.399326258101766	21.249529916718014
40	22.98190819383851	24.285252715006823	34.64915455632083	18.083684534833832
41	18.857887423661126	25.647390750879694	33.71975797552565	21.774963849933528
42	22.294218284606515	22.594541913773043	36.1357653485502	18.97547445307024
43	23.25822005723981	21.54473338994931	33.72222977494266	21.474816777868213
44	21.30532196070192	21.843644562306118	33.03453986571067	23.81649361128129
45	20.380339307437115	20.440898393153823	32.87316667520026	26.305595624208806
46	24.59493387053774	22.73102055301215	32.56578076198514	20.10826481446497
47	18.89637687172597	20.679780151097567	40.03908974220898	20.384753234967487
48	20.194248122756623	22.196052536331038	33.605348973938405	24.004350366973934
49	18.387362748923444	20.30477286811714	39.97694164258134	21.33092274037808
50	19.69088382719297	19.581948095743385	37.68858505373515	23.038583023328492
51	19.580888753136094	19.391619540633734	33.78843868789825	27.239053018331923
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	460.0
2	797.0
3	16379.0
4	31961.0
5	21865.0
6	11769.0
7	10813.0
8	9857.0
9	9479.0
10	9101.0
11	8700.5
12	8300.0
13	7932.0
14	7564.0
15	7030.5
16	6497.0
17	5894.0
18	5291.0
19	4827.5
20	4364.0
21	4157.0
22	3950.0
23	3858.5
24	3767.0
25	4074.0
26	4470.0
27	4559.0
28	5229.0
29	5899.0
30	6508.0
31	7117.0
32	7759.5
33	8402.0
34	9150.5
35	9899.0
36	10550.5
37	11202.0
38	12034.5
39	12867.0
40	13981.0
41	15095.0
42	16957.0
43	18819.0
44	22254.5
45	25690.0
46	55545.5
47	85401.0
48	64922.5
49	44444.0
50	43249.0
51	42054.0
52	36170.0
53	30286.0
54	27102.0
55	23918.0
56	22336.5
57	20755.0
58	19854.5
59	18954.0
60	18231.5
61	17509.0
62	16491.0
63	15473.0
64	13992.0
65	12511.0
66	10627.5
67	8744.0
68	7528.5
69	6313.0
70	5357.0
71	4401.0
72	3708.0
73	3015.0
74	2558.5
75	1739.5
76	1377.0
77	1106.5
78	836.0
79	661.5
80	487.0
81	375.5
82	264.0
83	192.5
84	121.0
85	98.5
86	76.0
87	52.5
88	29.0
89	24.0
90	19.0
91	17.5
92	16.0
93	12.5
94	9.0
95	5.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	566389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.36532098463208
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.75239159132676	35.12283951485409
2	9.258910704885766	8.58584733600149
3	4.605094990729632	6.405501222297021
4	3.017207147669692	5.595751115153258
5	2.1341466954035377	4.947519828033843
6	1.515386209228265	4.2156820803932
7	1.0826453374826102	3.513803900942758
8	0.7235893849833287	2.683956327665964
9	0.5071424520743011	2.116244031782253
>10	1.3174206279260445	9.069844302481316
>50	0.054275135903449706	1.702760989008268
>100	0.026362208808411506	2.288742281690388
>500	0.001550718165200677	0.3834043263053167
>1k	0.001938397706500846	2.037992616807164
>5k	7.753590826003384E-4	2.64716151593924
>10k+	0.0011630386239005076	8.682948610644457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19442	3.4326231618198797	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	18131	3.20115680212716	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCG	10733	1.894987367339408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGC	9024	1.593251281363162	No Hit
GAACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	5703	1.0069051482285143	No Hit
GCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGC	4034	0.7122313463008639	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTC	2188	0.386306937458178	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCT	2045	0.3610592719844488	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	1824	0.3220401526159583	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGATACGTTCGTA	1247	0.2201667052149671	No Hit
CTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCTGCT	602	0.10628737493136343	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7655710121488943E-4	0.0	0.0	0.13453651112574574	0.0
2	1.7655710121488943E-4	0.0	0.0	1.1308482332813667	0.0
3	1.7655710121488943E-4	0.0	0.0	1.3912699575733285	0.0
4	1.7655710121488943E-4	0.0	0.0	2.8725840367662507	0.0
5	1.7655710121488943E-4	0.0	0.0	7.523627754070082	0.0
6	1.7655710121488943E-4	0.0	0.0	8.689787407594427	0.0
7	1.7655710121488943E-4	0.0	0.0	9.77826193658422	0.0
8	1.7655710121488943E-4	0.0	0.0	11.174828607193996	0.0
9	1.7655710121488943E-4	0.0	0.0	11.840095764571698	0.0
10	1.7655710121488943E-4	0.0	0.0	14.944322718131884	0.0
11	1.7655710121488943E-4	0.0	0.0	15.858535388222583	0.0
12	1.7655710121488943E-4	0.0	0.0	18.543439226397407	0.0
13	1.7655710121488943E-4	0.0	0.0	19.090766240163564	0.0
14	1.7655710121488943E-4	0.0	0.0	19.377494972536542	0.0
15	1.7655710121488943E-4	0.0	0.0	19.871501741735802	0.0
16	1.7655710121488943E-4	0.0	0.0	20.35562131326703	0.0
17	1.7655710121488943E-4	0.0	0.0	20.861810522450117	0.0
18	1.7655710121488943E-4	0.0	0.0	21.47322776395728	0.0
19	1.7655710121488943E-4	0.0	0.0	21.877543525739377	0.0
20	1.7655710121488943E-4	0.0	0.0	22.239662140331117	0.0
21	1.7655710121488943E-4	0.0	0.0	22.61714122272855	0.0
22	1.7655710121488943E-4	0.0	0.0	23.016513385676628	0.0
23	1.7655710121488943E-4	0.0	0.0	23.3671557886894	0.0
24	1.7655710121488943E-4	0.0	0.0	23.647705022519858	0.0
25	1.7655710121488943E-4	0.0	0.0	23.906537732900887	0.0
26	1.7655710121488943E-4	0.0	0.0	24.168548471103783	0.0
27	1.7655710121488943E-4	0.0	0.0	24.443800991897795	0.0
28	1.7655710121488943E-4	0.0	0.0	24.715169256465078	0.0
29	1.7655710121488943E-4	0.0	0.0	24.992363905372457	0.0
30	1.7655710121488943E-4	0.0	0.0	25.338592380854855	0.0
31	1.7655710121488943E-4	0.0	0.0	25.620554071495032	0.0
32	1.7655710121488943E-4	0.0	0.0	25.919818358054272	0.0
33	1.7655710121488943E-4	0.0	0.0	26.219259201714724	0.0
34	1.7655710121488943E-4	0.0	0.0	26.510754975820504	0.0
35	1.7655710121488943E-4	0.0	0.0	26.80878336267124	5.296713036446682E-4
36	1.7655710121488943E-4	0.0	0.0	27.08544834027497	5.296713036446682E-4
37	1.7655710121488943E-4	0.0	0.0	27.361407089473843	5.296713036446682E-4
38	1.7655710121488943E-4	0.0	0.0	27.66755710298046	5.296713036446682E-4
39	1.7655710121488943E-4	0.0	0.0	27.981122514738104	5.296713036446682E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	20	7.030251E-4	45.000004	44
GTACGAG	20	7.030251E-4	45.000004	1
TTGTGCG	45	3.8380676E-10	45.0	1
CATAGCG	35	1.2104647E-7	45.0	1
CGTAAGG	25	3.888291E-5	45.0	2
GCATCGT	25	3.888291E-5	45.0	35
GCATATC	35	1.2104647E-7	45.0	28
CGCATCG	30	2.1635442E-6	44.999996	21
CATCGCC	30	2.1635442E-6	44.999996	23
ATCGCCA	30	2.1635442E-6	44.999996	24
CGTTTTT	10380	0.0	43.95954	1
TCGTTCG	65	0.0	41.53846	45
CTACGAA	65	0.0	41.53846	11
CGAATAT	55	6.002665E-11	40.909092	14
TACGGGA	130	0.0	39.80769	4
TACGGCT	1275	0.0	39.70588	7
CGGTCTA	40	3.454079E-7	39.375004	31
GAATCTG	2455	0.0	39.31772	1
ACACGAC	115	0.0	39.130432	26
AGACACG	115	0.0	39.130432	24
>>END_MODULE