FastQCFastQC Report
Sat 18 Jun 2016
SRR3551787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286861
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC136474.757356350288119No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG132854.631162827989862No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127814.45546797926522No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC95823.3402937311101897No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC33601.1712989914976242Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT30931.0782225537804022No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT30501.0632327154963555No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC27140.946102816346593No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA20620.7188150358536016No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC8250.287595734519506Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT7020.24471782500932507Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT6330.2206643635767846No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGT5420.18894168255705726No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTT5250.1830154674215038No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCC5200.18127246296987043No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG5180.18057526118921707No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC4800.16732842735680348No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT4530.15791620331798326No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTAT4340.15129278640177649No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC2920.10179145997538878Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGT207.024425E-445.0000044
CATTGCG207.024425E-445.0000041
ATAGCGG207.024425E-445.0000042
ATCACAT351.2076634E-745.00000432
TATGAGG207.024425E-445.0000042
CGGGCTA207.024425E-445.0000046
AGACCGG207.024425E-445.0000042
GGAACAC351.2076634E-745.0000048
GTACTTC207.024425E-445.0000049
GCGATGT207.024425E-445.0000049
AACTATG207.024425E-445.0000047
ACTCATC207.024425E-445.00000421
CACCCGT207.024425E-445.00000416
TGCGTAG207.024425E-445.0000041
ATGTGCG253.8834634E-545.01
TAGCCGT253.8834634E-545.044
ATGACGG453.8198777E-1045.02
TATACTC253.8834634E-545.018
TCGGCGT302.159788E-644.9999964
ATAGTTA302.159788E-644.99999637
Produced by FastQC (version 0.11.5)