##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551787_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286861
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.846319297499488	28.0	25.0	31.0	16.0	33.0
2	27.56390725821914	30.0	25.0	31.0	16.0	33.0
3	27.839695880583278	30.0	26.0	31.0	16.0	33.0
4	30.340339049225932	33.0	28.0	35.0	19.0	37.0
5	31.73176904493814	33.0	30.0	35.0	26.0	35.0
6	32.391234779213626	35.0	32.0	35.0	26.0	36.0
7	33.17282237738835	35.0	32.0	35.0	29.0	37.0
8	33.407580674961046	35.0	33.0	35.0	28.0	37.0
9	34.6574577931472	37.0	33.0	39.0	28.0	39.0
10	35.42412178720704	37.0	34.0	39.0	30.0	39.0
11	35.83330951227249	37.0	35.0	39.0	31.0	39.0
12	34.84161318548008	37.0	33.0	39.0	29.0	39.0
13	35.3419007812146	37.0	34.0	39.0	30.0	39.0
14	36.65773667385947	38.0	36.0	40.0	32.0	41.0
15	36.39563760845845	38.0	35.0	40.0	30.0	41.0
16	36.664489073105095	38.0	35.0	40.0	31.0	41.0
17	35.248123655707815	37.0	32.0	40.0	27.0	41.0
18	34.953925420325525	37.0	33.0	39.0	27.0	40.0
19	34.46514862598959	37.0	32.0	38.0	27.0	40.0
20	34.53211485702135	35.0	33.0	39.0	27.0	40.0
21	35.43814600102489	36.0	34.0	39.0	30.0	40.0
22	36.01163978372801	37.0	35.0	39.0	31.0	40.0
23	35.99320228263863	37.0	35.0	40.0	31.0	40.0
24	36.029278988778536	37.0	35.0	40.0	31.0	41.0
25	35.41139088269232	36.0	34.0	39.0	30.0	40.0
26	35.39080948612743	37.0	34.0	39.0	30.0	40.0
27	35.96608810538902	37.0	35.0	40.0	31.0	41.0
28	35.84437410453146	37.0	35.0	40.0	31.0	41.0
29	35.868504258159874	37.0	35.0	40.0	31.0	41.0
30	35.532470429929475	37.0	35.0	40.0	30.0	41.0
31	34.919748589037894	37.0	34.0	40.0	26.0	41.0
32	34.636478991567344	37.0	34.0	40.0	24.0	41.0
33	33.84603344476942	37.0	33.0	40.0	18.0	41.0
34	33.04128828945029	37.0	33.0	40.0	15.0	41.0
35	32.89205573431035	37.0	33.0	40.0	12.0	41.0
36	32.20326569314058	37.0	31.0	40.0	10.0	41.0
37	32.33471960287387	37.0	31.0	40.0	10.0	41.0
38	31.886014480880984	36.0	30.0	40.0	9.0	41.0
39	31.599635363468717	36.0	30.0	40.0	9.0	40.0
40	31.29983859778778	35.0	29.0	39.0	8.0	40.0
41	30.670446662320774	35.0	26.0	39.0	8.0	40.0
42	31.24742645392716	35.0	29.0	39.0	8.0	40.0
43	31.127668104064337	35.0	28.0	39.0	8.0	40.0
44	31.1134347297123	35.0	28.0	39.0	8.0	40.0
45	31.184542339321133	36.0	28.0	39.0	8.0	40.0
46	30.887628503003196	35.0	27.0	39.0	8.0	40.0
47	30.88870916576321	35.0	27.0	39.0	8.0	40.0
48	30.868891205148138	35.0	27.0	39.0	8.0	40.0
49	30.84803092787099	35.0	27.0	39.0	8.0	40.0
50	30.834031116115472	35.0	27.0	39.0	8.0	40.0
51	27.43274965924263	31.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	10.0
11	3.0
12	9.0
13	11.0
14	24.0
15	32.0
16	101.0
17	220.0
18	404.0
19	690.0
20	1017.0
21	1536.0
22	2333.0
23	3506.0
24	5361.0
25	8303.0
26	11460.0
27	12240.0
28	11457.0
29	10430.0
30	10660.0
31	12302.0
32	15509.0
33	19515.0
34	25576.0
35	37785.0
36	42764.0
37	36999.0
38	15398.0
39	1203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68631497484844	10.340548209760128	13.104256068269999	32.86888074712142
2	38.43952297454168	35.58971069612112	14.569425610313008	11.401340719024196
3	18.865931583589266	33.14776145938277	37.5589571255765	10.427349831451469
4	18.338847037415334	13.757534136742185	55.61090563025298	12.292713195589501
5	28.337069172874667	16.332300312694997	37.700837687939455	17.629792826490878
6	15.693663481616532	32.494134790020254	41.5305670690683	10.28163465929492
7	47.66141092724351	3.984856777324209	43.26764530556611	5.086086989866172
8	41.77946810476154	15.371904859845012	34.72134587831737	8.127281157076077
9	38.491813108090675	5.337776832682031	33.081875891111025	23.088534168116265
10	27.3494828505792	15.250940350901656	40.81140343232437	16.588173366194777
11	21.63103384566044	15.846002070689288	43.8125084971467	18.710455586503567
12	19.526878871648638	11.726585349698983	49.26288341740425	19.48365236124813
13	16.003569673116946	14.979380257337176	56.24745085598949	12.76959921355639
14	15.613833877731723	23.074590132503197	41.3684676550664	19.943108334698685
15	10.779088129791084	16.3479873527597	58.94562174711794	13.927302770331277
16	11.672552211698349	20.9509135086331	40.97629165344888	26.400242626219665
17	12.338031311331969	17.63676484429741	48.577882667912334	21.447321176458285
18	12.808293912382652	19.99121525756377	47.22252240632223	19.977968423731355
19	13.072184786359944	22.02425564994893	42.0363869609323	22.867172602758828
20	13.250319841316873	21.687158589003037	50.503205385186554	14.559316184493534
21	13.900111900885795	26.1105552863582	44.262203645668116	15.727129167087892
22	11.333363545410496	17.099919473194333	43.60439376562168	27.962323215773495
23	13.02756387239813	25.14667382460495	45.56562237459955	16.260139928397376
24	18.968768846235633	16.642555105085737	39.02552107118081	25.363154977497814
25	11.90297740020428	30.402180847169884	38.68180059331871	19.01304115930712
26	13.470635604003334	16.080959070769467	45.512983640160215	24.935421685066984
27	19.202331442754506	19.896047214504588	37.46866949498189	23.43295184775902
28	12.844199804086301	16.744695165951455	48.065090758241794	22.34601427172045
29	18.618076350567	14.276252261548276	43.20803455332025	23.897636834564477
30	15.046311628279899	20.571984340848008	41.8129337902329	22.568770240639193
31	23.226928721575955	17.13164215421406	36.07740334168813	23.56402578252185
32	20.30844206776104	19.74963484056738	36.46365312817009	23.478269963501486
33	14.526199099912501	17.160576028111176	40.86264776320239	27.45057710877394
34	20.384785662742583	22.15951279539568	39.60594155357473	17.84975998828701
35	20.05988963295812	17.3634617462813	33.595713603452545	28.980935017308035
36	21.406186271399736	18.442381501842355	43.26415929666285	16.88727293009506
37	17.26585349698983	19.302728499168587	36.71778317721823	26.713634826623345
38	22.018678035703704	19.071954709772328	35.67651231781246	23.232854936711508
39	21.01610187512419	22.011357417006845	35.51929331627513	21.453247391593838
40	22.280825905229364	18.24786220504007	41.55392332872018	17.917388561010387
41	16.93503125206982	27.432798463367273	36.13561969037269	19.496550594190218
42	22.070619568362375	17.635370440736107	42.00954469237714	18.28446529852437
43	22.353683491307635	17.308731406500012	36.705582146056805	23.63200295613555
44	20.74767918957265	18.053342908237788	35.824667696201296	25.374310205988266
45	18.33466382673141	15.905264222044824	35.889507461802054	29.870564489421707
46	26.277186511934353	21.05444797306012	33.94117708576628	18.72718842923925
47	14.503888642931596	16.199831974370863	49.16736677345474	20.128912609242803
48	20.548628081196117	19.569408180268493	33.894115965572176	25.987847772963214
49	16.80395731730699	14.991232687608283	45.789772747079596	22.41503724800513
50	19.121804637089042	14.119381860901273	40.700199748310155	26.05861375369953
51	18.68709932685168	14.913146088175107	34.30546501615765	32.09428956881556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	301.5
2	476.0
3	10152.0
4	19828.0
5	13325.5
6	6823.0
7	6417.5
8	6012.0
9	5832.5
10	5653.0
11	5471.5
12	5290.0
13	4984.5
14	4679.0
15	4248.0
16	3817.0
17	3502.0
18	3187.0
19	2895.0
20	2603.0
21	2343.5
22	2084.0
23	1923.0
24	1762.0
25	1748.0
26	1826.5
27	1919.0
28	1942.0
29	1965.0
30	2162.5
31	2360.0
32	2514.0
33	2668.0
34	2824.5
35	2981.0
36	3186.5
37	3392.0
38	3601.5
39	3811.0
40	4587.0
41	5363.0
42	6424.0
43	7485.0
44	9779.5
45	12074.0
46	39416.5
47	66759.0
48	46623.5
49	26488.0
50	25166.0
51	23844.0
52	18950.5
53	14057.0
54	11933.0
55	9809.0
56	8622.0
57	7435.0
58	6981.0
59	6527.0
60	6076.0
61	5625.0
62	5202.5
63	4780.0
64	4225.0
65	3670.0
66	3164.5
67	2659.0
68	2329.5
69	2000.0
70	1726.5
71	1453.0
72	1322.5
73	1192.0
74	1048.5
75	726.0
76	547.0
77	477.5
78	408.0
79	337.5
80	267.0
81	214.0
82	161.0
83	122.0
84	83.0
85	67.0
86	51.0
87	36.0
88	21.0
89	18.5
90	16.0
91	11.5
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.51140489245361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70662224383682	39.637030340655244
2	8.891216579092308	8.626508149096864
3	3.688413400545518	5.367903476538459
4	1.982069635077871	3.8461193036920145
5	1.260561089237267	3.05757946958307
6	0.7431829435936663	2.163170921150272
7	0.4740714854449666	1.6098511644871392
8	0.2807513912813023	1.089571553325354
9	0.17943425926474196	0.7834147202492455
>10	0.7032399779267532	6.202844728127798
>50	0.05570918873446506	1.8387740215909714
>100	0.02315187064289457	1.9672319891233814
>500	0.005064471703133187	1.4969213976112794
>1k	0.003617479787952276	5.011615624030823
>5k	7.234959575904553E-4	3.363071707364896
>10k+	0.0021704878727713656	13.938391433373209
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	13647	4.757356350288119	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	13285	4.631162827989862	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12781	4.45546797926522	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	9582	3.3402937311101897	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	3360	1.1712989914976242	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	3093	1.0782225537804022	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	3050	1.0632327154963555	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	2714	0.946102816346593	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	2062	0.7188150358536016	No Hit
CCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	825	0.287595734519506	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGCT	702	0.24471782500932507	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT	633	0.2206643635767846	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGT	542	0.18894168255705726	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTT	525	0.1830154674215038	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCC	520	0.18127246296987043	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATG	518	0.18057526118921707	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	480	0.16732842735680348	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT	453	0.15791620331798326	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTAT	434	0.15129278640177649	No Hit
TCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	292	0.10179145997538878	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3004939674615929	0.0
2	0.0	0.0	0.0	2.173177950296485	0.0
3	0.0	0.0	0.0	2.610672067656461	0.0
4	0.0	0.0	0.0	4.439432338310192	0.0
5	0.0	0.0	0.0	11.454328054353851	0.0
6	0.0	0.0	0.0	12.590418355928481	0.0
7	0.0	0.0	0.0	14.683766702340158	0.0
8	0.0	0.0	0.0	16.975817556238038	0.0
9	0.0	0.0	0.0	17.62247220779402	0.0
10	0.0	0.0	0.0	24.10993477677342	0.0
11	0.0	0.0	0.0	25.726745706108535	0.0
12	0.0	0.0	0.0	31.078815175293958	0.0
13	0.0	0.0	0.0	31.966701642955996	0.0
14	0.0	0.0	0.0	32.392343330044866	0.0
15	0.0	0.0	0.0	33.593621998110585	0.0
16	0.0	0.0	0.0	34.54774263493469	0.0
17	0.0	0.0	0.0	35.48373602546181	0.0
18	0.0	0.0	0.0	36.577645619306914	0.0
19	0.0	0.0	0.0	37.36304342521291	0.0
20	0.0	0.0	0.0	38.00690926964627	0.0
21	0.0	0.0	0.0	38.62358424463416	0.0
22	0.0	0.0	0.0	39.2657070846159	0.0
23	0.0	0.0	0.0	39.77919619606708	0.0
24	0.0	0.0	0.0	40.20274627781399	0.0
25	0.0	0.0	0.0	40.550301365469686	0.0
26	0.0	0.0	0.0	40.909360282506164	0.0
27	0.0	0.0	0.0	41.29421566542681	0.0
28	0.0	0.0	0.0	41.62329490589519	0.0
29	0.0	0.0	0.0	41.954814352595854	0.0
30	0.0	0.0	0.0	42.367906407632965	0.0
31	3.486008903266739E-4	0.0	0.0	42.70709507392082	0.0
32	3.486008903266739E-4	0.0	0.0	43.0518613544539	0.0
33	3.486008903266739E-4	0.0	0.0	43.37780318690934	0.0
34	3.486008903266739E-4	0.0	0.0	43.682131764164524	0.0
35	3.486008903266739E-4	0.0	0.0	43.995872565458534	0.0
36	3.486008903266739E-4	0.0	0.0	44.28555990532	0.0
37	3.486008903266739E-4	0.0	0.0	44.583613666549304	0.0
38	3.486008903266739E-4	0.0	0.0	44.90084047674658	0.0
39	3.486008903266739E-4	0.0	0.0	45.23549733146019	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGT	20	7.024425E-4	45.000004	4
CATTGCG	20	7.024425E-4	45.000004	1
ATAGCGG	20	7.024425E-4	45.000004	2
ATCACAT	35	1.2076634E-7	45.000004	32
TATGAGG	20	7.024425E-4	45.000004	2
CGGGCTA	20	7.024425E-4	45.000004	6
AGACCGG	20	7.024425E-4	45.000004	2
GGAACAC	35	1.2076634E-7	45.000004	8
GTACTTC	20	7.024425E-4	45.000004	9
GCGATGT	20	7.024425E-4	45.000004	9
AACTATG	20	7.024425E-4	45.000004	7
ACTCATC	20	7.024425E-4	45.000004	21
CACCCGT	20	7.024425E-4	45.000004	16
TGCGTAG	20	7.024425E-4	45.000004	1
ATGTGCG	25	3.8834634E-5	45.0	1
TAGCCGT	25	3.8834634E-5	45.0	44
ATGACGG	45	3.8198777E-10	45.0	2
TATACTC	25	3.8834634E-5	45.0	18
TCGGCGT	30	2.159788E-6	44.999996	4
ATAGTTA	30	2.159788E-6	44.999996	37
>>END_MODULE