FastQCFastQC Report
Sat 18 Jun 2016
SRR3551785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282717
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98893.4978441338865367No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC94513.342918890622071No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG75162.6584888775701496No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC57742.04232501052289No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT25510.9023157433051426No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC20000.7074212021208488Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT14660.5185397411545821No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC14510.5132340821386758No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA10290.36396820849117667No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT5220.18463693375354154No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT4910.17367190512066838Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC3160.1117725499350941No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGT3140.11106512873297325No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC2930.10363720611070434Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAC700.045.00000426
CACGACC700.045.00000427
AAACACG700.045.00000440
TCACGAC207.024253E-445.025
TCGCAAG253.8833197E-545.01
CGACGGT207.024253E-445.028
TTCCGAC207.024253E-445.010
CACGACG207.024253E-445.026
ACGATAG253.8833197E-545.01
CGTTTTT49900.044.053111
GACACGA750.042.025
CGACCAA750.042.029
GAATGAC1400.041.7857171
AACACGT700.041.78571741
ACGGGCT501.0750227E-940.55
TACGGCT7150.039.650357
GTAGGGC403.4458935E-739.3754
GAGACAC800.039.37523
AGACACG800.039.37524
CGTAAGG403.4458935E-739.3752
Produced by FastQC (version 0.11.5)