##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282717
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.546298949125806	28.0	25.0	31.0	16.0	33.0
2	27.516081452477213	30.0	25.0	31.0	16.0	33.0
3	27.757400510050687	30.0	25.0	33.0	16.0	33.0
4	30.595132234708206	33.0	28.0	35.0	19.0	37.0
5	31.742700297470616	33.0	30.0	35.0	26.0	35.0
6	32.08428569912669	35.0	32.0	35.0	25.0	37.0
7	33.07647223194926	35.0	32.0	35.0	28.0	37.0
8	33.717572696371285	35.0	33.0	35.0	30.0	37.0
9	34.84755426804897	37.0	34.0	39.0	28.0	39.0
10	35.27323082800115	37.0	34.0	39.0	30.0	39.0
11	35.417703923004275	37.0	34.0	39.0	30.0	39.0
12	34.44922661177078	35.0	32.0	39.0	27.0	39.0
13	34.99387373238963	37.0	34.0	39.0	30.0	39.0
14	36.35654382297492	38.0	35.0	40.0	31.0	41.0
15	36.3734830236597	38.0	35.0	40.0	30.0	41.0
16	36.603794607328176	38.0	35.0	40.0	31.0	41.0
17	35.14582426949918	37.0	32.0	40.0	27.0	41.0
18	34.92314222349558	37.0	33.0	39.0	27.0	40.0
19	34.56823608060357	37.0	32.0	39.0	27.0	40.0
20	34.6842885288115	35.0	33.0	39.0	27.0	40.0
21	35.507029998196074	37.0	34.0	39.0	30.0	40.0
22	36.07134342823389	37.0	35.0	39.0	31.0	40.0
23	36.14844526505304	38.0	35.0	40.0	31.0	41.0
24	36.18011651227199	38.0	35.0	40.0	31.0	41.0
25	35.54995631674077	37.0	34.0	40.0	30.0	41.0
26	35.54845304668626	37.0	34.0	40.0	30.0	41.0
27	36.07855558738951	38.0	35.0	40.0	31.0	41.0
28	36.03735183947198	38.0	35.0	40.0	31.0	41.0
29	36.14253122380331	38.0	35.0	40.0	31.0	41.0
30	35.73898279905347	38.0	35.0	40.0	30.0	41.0
31	35.476200582207646	38.0	35.0	40.0	29.0	41.0
32	35.13961311134456	38.0	35.0	40.0	27.0	41.0
33	34.56278540024123	38.0	34.0	40.0	22.0	41.0
34	33.95315103088954	37.0	33.0	40.0	18.0	41.0
35	33.758489938701956	37.0	33.0	40.0	17.0	41.0
36	33.41859881082496	37.0	33.0	40.0	15.0	41.0
37	33.159608371622504	37.0	33.0	40.0	14.0	41.0
38	33.10176253992508	37.0	33.0	40.0	12.0	41.0
39	32.72666305881854	36.0	32.0	40.0	12.0	40.0
40	32.52503740489606	36.0	31.0	39.0	10.0	40.0
41	32.415928295786955	36.0	31.0	39.0	10.0	40.0
42	32.438194378123704	36.0	31.0	40.0	10.0	41.0
43	32.47504394854218	36.0	32.0	40.0	10.0	40.0
44	32.25408093605974	36.0	31.0	39.0	10.0	40.0
45	32.33964706756226	36.0	31.0	40.0	10.0	40.0
46	32.075665771778844	35.0	31.0	39.0	10.0	40.0
47	32.06739955503206	35.0	31.0	39.0	10.0	40.0
48	32.124831545326245	35.0	31.0	39.0	10.0	40.0
49	32.01048398221543	35.0	31.0	39.0	10.0	40.0
50	31.923425191976428	35.0	31.0	39.0	10.0	40.0
51	29.345292288755186	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	4.0
10	14.0
11	10.0
12	11.0
13	11.0
14	31.0
15	70.0
16	102.0
17	244.0
18	464.0
19	645.0
20	1011.0
21	1398.0
22	1978.0
23	2772.0
24	4274.0
25	6562.0
26	9083.0
27	9976.0
28	9381.0
29	9039.0
30	9597.0
31	11892.0
32	15438.0
33	19936.0
34	25560.0
35	36191.0
36	42838.0
37	41448.0
38	20865.0
39	1866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66292440850744	12.21893271363236	16.120714353929902	30.997428523930292
2	37.76709571762575	29.492743627019248	18.806792658382765	13.933367996972237
3	23.592497090730305	27.94667458978413	35.78808490469268	12.672743414792883
4	20.941436135782425	18.2023012411705	46.07009836691815	14.78616425612892
5	26.062811928536313	20.916322683107136	35.37919544986683	17.641669938489727
6	19.322856425329924	31.387925027501	37.311516463459924	11.97770208370915
7	58.469777197692395	4.625473530067169	32.52333605690496	4.381413215335477
8	55.66626697368747	10.818946154635201	27.47694691157589	6.037839960101444
9	51.00153156690259	5.781753484933697	27.1398607087653	16.076854239398408
10	31.11486044348235	19.83821277107496	33.97107354704528	15.075853238397407
11	25.493691571430087	18.557780395236225	38.14874945616995	17.799778577163735
12	22.52782818153843	15.482266719015836	42.73036287170563	19.259542227740106
13	19.83043113785163	17.690835712037124	47.0251169897813	15.453616160329942
14	18.00280846217242	23.24444585928685	38.98138421106619	19.77136146747454
15	15.466349741968116	18.42867602584917	49.65389417686237	16.45108005532034
16	18.054803920528304	21.1526013646155	38.024950745798805	22.767643969057396
17	17.65970917914381	19.238673302277544	41.88110371855955	21.2205138000191
18	18.945800924599514	20.20182726896508	40.35519618558488	20.497175620850534
19	18.641609807687544	22.2367243568657	37.317175833076895	21.80449000236986
20	19.464340665754094	21.85082609110878	41.84467152665032	16.840161716486804
21	19.32073416172356	24.38693110071202	39.065567334118576	17.226767403445848
22	17.706398978483783	18.394719808147368	39.03125740581571	24.867623807553134
23	18.282239837010156	23.583300615102733	40.20840628614481	17.926053261742307
24	21.690595188828404	18.325038819738467	36.882819214974695	23.101546776458438
25	17.150365913616795	26.284942186002258	35.78560893048526	20.77908296989569
26	17.09554077045243	18.41877212901948	39.244898608856204	25.240788491671886
27	21.055684659924943	20.05362252712076	36.301318986831355	22.589373826122944
28	15.654170071131201	19.582833717109335	41.799042859113534	22.963953352645934
29	21.52824202294167	17.68517634242016	36.10748557745024	24.67909605718793
30	18.12589975134145	21.348557037602976	42.63486100941931	17.890682201636267
31	21.66265205134463	18.86020295914289	38.28245206337079	21.19469292614169
32	26.351793489602677	21.89468620564027	34.10371502244294	17.649805282314116
33	19.23619732807012	23.9875918321148	33.71321851887223	23.06299232094285
34	21.77053378466806	24.61896525500766	33.27532479475943	20.335176165564857
35	24.20194045635742	22.075786033383206	33.21448657137703	20.507786938882344
36	23.47082064396552	23.751666861207497	32.998369394129114	19.779143100697873
37	23.950452219003456	22.56284553104341	35.63280595082715	17.85389629912598
38	22.133087150754996	25.933000137947133	30.666709111938793	21.26720359935908
39	27.140568129967424	23.97096743386496	30.100064729039993	18.788399707127624
40	24.327154009132805	24.04453923888553	33.40407545354542	18.224231298436244
41	22.358400803630484	27.400191711145776	30.00633141975898	20.235076065464757
42	24.488092332615302	21.163566393248374	34.8574723133027	19.490868960833623
43	26.333400538347533	20.534668944562938	30.074951276364704	23.05697924072482
44	22.311003583088386	21.008287439382844	32.64890332028141	24.031805657247354
45	21.33476232416162	19.920627341122042	32.62343615700506	26.121174177711282
46	26.35533059561328	23.049197607501494	30.871153839351717	19.724317957533504
47	19.076673846991866	20.258774675735808	40.256510927889025	20.408040549383305
48	22.114694199499855	21.726673670136567	31.984634811489936	24.173997318873646
49	20.301219947863057	19.367070250462476	38.42075290838542	21.91095689328905
50	20.863973514150192	19.066769950162175	35.87828110796309	24.190975427724545
51	20.71081682389103	18.98753877552464	32.03875253345218	28.26289186713215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	305.5
2	495.0
3	8496.0
4	16497.0
5	11025.5
6	5554.0
7	5142.5
8	4731.0
9	4533.5
10	4336.0
11	4181.0
12	4026.0
13	3754.0
14	3482.0
15	3177.5
16	2873.0
17	2747.5
18	2622.0
19	2438.5
20	2255.0
21	2129.0
22	2003.0
23	1939.0
24	1875.0
25	1826.5
26	1799.5
27	1821.0
28	2100.0
29	2379.0
30	2574.0
31	2769.0
32	3050.5
33	3332.0
34	3649.0
35	3966.0
36	4259.5
37	4553.0
38	4862.0
39	5171.0
40	5846.0
41	6521.0
42	7560.0
43	8599.0
44	10208.0
45	11817.0
46	28666.0
47	45515.0
48	32999.0
49	20483.0
50	20154.5
51	19826.0
52	17337.5
53	14849.0
54	13717.0
55	12585.0
56	11919.5
57	11254.0
58	10721.5
59	10189.0
60	10028.5
61	9868.0
62	9361.5
63	8855.0
64	7835.0
65	6815.0
66	5875.5
67	4936.0
68	4369.5
69	3803.0
70	3326.5
71	2850.0
72	2630.0
73	2410.0
74	2009.5
75	1446.0
76	1283.0
77	1061.5
78	840.0
79	664.5
80	489.0
81	404.5
82	320.0
83	248.0
84	176.0
85	135.5
86	95.0
87	72.5
88	50.0
89	36.0
90	22.0
91	17.0
92	12.0
93	10.5
94	9.0
95	5.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	282717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.364299439784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00601731250875	36.47336062654855
2	8.696810077123372	8.238366333276046
3	4.427104621180514	6.290601267865361
4	2.9025489984763073	5.499087996099078
5	1.9988706731832662	4.733755455302742
6	1.4446266702855783	4.105423811406254
7	0.9698303725547528	3.2154735320036405
8	0.6753669525181263	2.559062605664233
9	0.5131005250298515	2.1872382219201842
>10	1.3061149950672224	9.221937474593647
>50	0.035463464523995236	1.1071738871580628
>100	0.016599919564423304	1.4838560295288175
>500	7.545417983828773E-4	0.18655415400145536
>1k	0.0037727089919143866	3.0366870623570232
>5k	0.0030181671935315094	11.661421542274883
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9889	3.4978441338865367	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	9451	3.342918890622071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCG	7516	2.6584888775701496	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGC	5774	2.04232501052289	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCT	2551	0.9023157433051426	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	2000	0.7074212021208488	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCT	1466	0.5185397411545821	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTC	1451	0.5132340821386758	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTA	1029	0.36396820849117667	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTT	522	0.18463693375354154	No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	491	0.17367190512066838	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	316	0.1117725499350941	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGT	314	0.11106512873297325	No Hit
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	293	0.10363720611070434	Illumina Single End Adapter 1 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20373730621080444	0.0
2	0.0	0.0	0.0	1.2217164160627059	0.0
3	0.0	0.0	0.0	1.5209555845598248	0.0
4	0.0	0.0	0.0	2.804571355808105	0.0
5	0.0	0.0	0.0	7.238687450701585	0.0
6	0.0	0.0	0.0	7.999518953582558	0.0
7	0.0	0.0	0.0	9.124672375555768	0.0
8	0.0	0.0	0.0	10.494239822861731	0.0
9	0.0	0.0	0.0	10.998277429372836	0.0
10	0.0	0.0	0.0	14.61885914182734	0.0
11	0.0	0.0	0.0	15.498183696063554	0.0
12	0.0	0.0	0.0	18.592444034140147	0.0
13	0.0	0.0	0.0	19.1049706950767	0.0
14	0.0	0.0	0.0	19.358934906638087	0.0
15	0.0	0.0	0.0	19.989954618929882	0.0
16	0.0	0.0	0.0	20.47842895899433	0.0
17	0.0	0.0	0.0	20.964073614250292	0.0
18	0.0	0.0	0.0	21.59509332654209	0.0
19	0.0	0.0	0.0	22.05244113371322	0.0
20	0.0	0.0	0.0	22.456732350725282	0.0
21	0.0	0.0	0.0	22.80301502916344	0.0
22	0.0	0.0	0.0	23.184315057106577	0.0
23	0.0	0.0	0.0	23.51220478428959	0.0
24	0.0	0.0	0.0	23.793404712132627	0.0
25	0.0	0.0	0.0	24.025085155827206	0.0
26	0.0	0.0	0.0	24.258534152527087	0.0
27	0.0	0.0	0.0	24.531952447146793	0.0
28	0.0	0.0	0.0	24.78061099969227	0.0
29	0.0	0.0	0.0	25.033867790051534	0.0
30	0.0	0.0	0.0	25.351146199202738	0.0
31	0.0	0.0	0.0	25.59449909273231	0.0
32	0.0	0.0	0.0	25.841742802873544	0.0
33	0.0	0.0	0.0	26.067763876951155	0.0
34	0.0	0.0	0.0	26.298383188842553	0.0
35	0.0	0.0	0.0	26.531124764340312	0.0
36	0.0	0.0	0.0	26.75360873240732	0.0
37	0.0	0.0	0.0	26.99731533653795	0.0
38	0.0	0.0	0.0	27.2399608088654	0.0
39	0.0	0.0	0.0	27.507012312666024	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	70	0.0	45.000004	26
CACGACC	70	0.0	45.000004	27
AAACACG	70	0.0	45.000004	40
TCACGAC	20	7.024253E-4	45.0	25
TCGCAAG	25	3.8833197E-5	45.0	1
CGACGGT	20	7.024253E-4	45.0	28
TTCCGAC	20	7.024253E-4	45.0	10
CACGACG	20	7.024253E-4	45.0	26
ACGATAG	25	3.8833197E-5	45.0	1
CGTTTTT	4990	0.0	44.05311	1
GACACGA	75	0.0	42.0	25
CGACCAA	75	0.0	42.0	29
GAATGAC	140	0.0	41.785717	1
AACACGT	70	0.0	41.785717	41
ACGGGCT	50	1.0750227E-9	40.5	5
TACGGCT	715	0.0	39.65035	7
GTAGGGC	40	3.4458935E-7	39.375	4
GAGACAC	80	0.0	39.375	23
AGACACG	80	0.0	39.375	24
CGTAAGG	40	3.4458935E-7	39.375	2
>>END_MODULE