SRR3551784_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355665
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	9394	2.641249490391239	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	9047	2.5436857717233914	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	7428	2.088482139091561	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	3270	0.9194044958036355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2697	0.7582978364472186	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT	2315	0.650893396876274	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	1885	0.5299931114953679	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTA	1531	0.43046124864690083	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	1311	0.36860528868457676	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC	1207	0.3393642894296599	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	711	0.19990721606005651	No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	695	0.1954086007900693	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	639	0.17966344734511408	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	618	0.17375901480325587	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	522	0.14676732318333263	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	517	0.14536150591146163	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	505	0.14198754445897122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC	467	0.1313033331927516	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATG	448	0.1259612275596418	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	447	0.1256800641052676	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	433	0.12174377574402878	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGACAGAG	430	0.12090028538090619	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	377	0.10599862229907356	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	25	3.885354E-5	45.000004	5
GCGAACC	30	2.1612595E-6	45.000004	33
GGATACA	30	2.1612595E-6	45.000004	8
TCGATGG	25	3.885354E-5	45.000004	2
ACGATAG	30	2.1612595E-6	45.000004	1
CGGCGAA	40	6.7921064E-9	45.0	31
ATTACGG	20	7.026711E-4	45.0	2
CGCGCAG	20	7.026711E-4	45.0	41
TAACCGC	20	7.026711E-4	45.0	37
AAGCATG	20	7.026711E-4	45.0	3
CGAATAC	20	7.026711E-4	45.0	45
GACGCGT	20	7.026711E-4	45.0	45
CGATCGA	35	1.208773E-7	45.0	41
ACGACGA	20	7.026711E-4	45.0	38
GCATATC	55	1.8189894E-12	45.0	28
CGATGAA	950	0.0	43.81579	19
GATACCT	980	0.0	43.622448	5
GATGAAT	990	0.0	43.181816	20
TGATACC	985	0.0	43.172592	4
TACGGCT	1140	0.0	43.026318	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content