##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	355665
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126214274668577	31.0	30.0	33.0	28.0	34.0
2	31.041027371262285	31.0	31.0	34.0	28.0	34.0
3	30.91764160094471	31.0	30.0	34.0	27.0	34.0
4	34.883435255085544	35.0	35.0	37.0	32.0	37.0
5	34.564390648503505	35.0	35.0	37.0	32.0	37.0
6	34.761604318670656	35.0	35.0	37.0	32.0	37.0
7	35.19769445967413	36.0	35.0	37.0	32.0	37.0
8	34.27334148707351	35.0	35.0	37.0	30.0	37.0
9	36.17796803171524	39.0	35.0	39.0	32.0	39.0
10	36.100934868485794	37.0	35.0	39.0	32.0	39.0
11	36.55534562017629	38.0	35.0	39.0	32.0	39.0
12	36.661113688442775	39.0	35.0	39.0	32.0	39.0
13	36.58648728438278	39.0	35.0	39.0	32.0	39.0
14	37.61350990398268	39.0	36.0	41.0	32.0	41.0
15	37.58132793499501	39.0	36.0	41.0	32.0	41.0
16	37.864296458746296	39.0	37.0	41.0	33.0	41.0
17	37.507418497743664	39.0	36.0	41.0	32.0	41.0
18	37.581429153838585	39.0	36.0	40.0	33.0	41.0
19	37.52663883148468	39.0	36.0	40.0	32.0	41.0
20	37.20439458479187	39.0	35.0	40.0	32.0	41.0
21	37.33004090928261	39.0	35.0	40.0	32.0	41.0
22	37.386231425639295	39.0	35.0	41.0	32.0	41.0
23	37.36565869568273	39.0	35.0	41.0	32.0	41.0
24	37.30002389889362	39.0	35.0	41.0	32.0	41.0
25	37.07490194424529	39.0	35.0	40.0	32.0	41.0
26	37.08400320526338	39.0	35.0	40.0	32.0	41.0
27	37.043110792459196	39.0	35.0	41.0	31.0	41.0
28	36.866332082155964	39.0	35.0	41.0	31.0	41.0
29	36.90411201552022	39.0	35.0	41.0	31.0	41.0
30	36.61750804830388	39.0	35.0	40.0	30.0	41.0
31	36.62620443394768	39.0	35.0	40.0	30.0	41.0
32	36.41961677421169	39.0	35.0	40.0	30.0	41.0
33	36.34290413732023	39.0	35.0	41.0	30.0	41.0
34	36.12515710008013	39.0	35.0	41.0	29.0	41.0
35	36.152202212756386	39.0	35.0	41.0	29.0	41.0
36	36.086109119536644	39.0	35.0	40.0	29.0	41.0
37	35.93755078514894	39.0	35.0	41.0	29.0	41.0
38	35.59456792206149	39.0	35.0	40.0	26.0	41.0
39	35.71767252892469	39.0	35.0	40.0	27.0	41.0
40	35.56248998355194	39.0	35.0	40.0	26.0	41.0
41	35.40940491754882	38.0	35.0	40.0	25.0	41.0
42	35.433101373483474	38.0	35.0	40.0	26.0	41.0
43	35.514239523146784	38.0	35.0	40.0	27.0	41.0
44	35.66840144518016	39.0	35.0	40.0	27.0	41.0
45	35.73850955252836	39.0	35.0	40.0	28.0	41.0
46	35.662457649754685	39.0	35.0	40.0	27.0	41.0
47	35.412688906695905	38.0	35.0	40.0	26.0	41.0
48	35.457152095370645	38.0	35.0	40.0	26.0	41.0
49	35.51924985590373	38.0	35.0	40.0	27.0	41.0
50	35.40227742398043	38.0	35.0	40.0	26.0	41.0
51	34.51406239017052	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	18.0
10	17.0
11	19.0
12	15.0
13	19.0
14	21.0
15	24.0
16	47.0
17	73.0
18	167.0
19	403.0
20	1083.0
21	1789.0
22	2125.0
23	2738.0
24	3343.0
25	3785.0
26	4194.0
27	4538.0
28	4825.0
29	5465.0
30	6705.0
31	8518.0
32	11149.0
33	15463.0
34	23810.0
35	27714.0
36	30825.0
37	46385.0
38	76440.0
39	73924.0
40	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.10145783251093	17.204672936611697	18.463441721844994	13.230427509032374
2	29.56489955435592	35.373736521726904	19.02323815950403	16.03812576441314
3	28.730968748682045	34.284228136026876	23.251936513292	13.732866601999072
4	26.901438151069122	20.900004217451816	35.67711188899667	16.521445742482392
5	33.41093444674061	26.073130614482732	21.644525044634698	18.871409894141962
6	22.41969268834437	41.23177709361337	23.559529332377377	12.78900088566488
7	64.3602828504351	7.098533732585438	23.52213459294561	5.0190488240338516
8	61.082479299340676	18.640855861555114	13.624618672065006	6.652046167039208
9	54.79116584426357	10.484022886705185	14.82574894915159	19.89906231987966
10	40.426243796831294	21.3273726681006	22.72503619979475	15.521347335273362
11	33.14635963617449	22.711259190530413	26.226364697116665	17.916016476178427
12	26.241547523652876	20.3596080581446	28.949432752730804	24.44941166547172
13	25.769755247212967	23.512575035496887	33.53324054939339	17.184429167896756
14	18.936077488648024	31.535855369519073	27.1120295783954	22.416037563437506
15	17.435226969198546	25.538638887717376	35.540466450170804	21.485667692913275
16	18.93495283483053	26.932647294504658	26.500780228585892	27.631619642078924
17	18.585185497589023	30.711484121293914	30.264434228838937	20.438896152278126
18	17.817046940238708	27.440990820013216	30.185989625068533	24.555972614679543
19	20.303656530724137	26.231144475841027	27.115684703302268	26.349514290132568
20	22.415475236528756	27.380821840777138	33.91084306861794	16.292859854076166
21	21.61331590119916	33.526492626488405	27.637805238075153	17.222386234237273
22	19.569819914807475	24.323169274457708	32.15329031532481	23.953720495410007
23	23.172367255704103	27.934995009348683	29.897797084334975	18.994840650612232
24	21.214907286350922	27.43368057019949	27.985042104227293	23.3663700392223
25	17.597177118918083	36.27036677772623	25.80012090028538	20.332335203070304
26	17.885088496197266	27.756456215821068	31.276060337677308	23.082394950304362
27	25.256350779525675	28.104255408881958	26.192062755683015	20.447331055909356
28	16.956686769853654	30.58327358609928	31.12957417794835	21.330465466098715
29	20.983509763400953	27.121026808935373	28.48635654337649	23.40910688428718
30	20.52830613076912	33.80906189813448	27.209874460517625	18.452757510578774
31	23.836475334935965	28.26479974132962	23.39589220193159	24.502832721802818
32	25.105084841072355	30.883556155370922	23.353717683775464	20.657641319781256
33	23.33937834760238	28.6224396552936	22.75034091068843	25.287841086415586
34	23.73019554918252	29.43078458661943	24.599552950107544	22.239466914090507
35	25.604149972586566	27.995726315493513	24.062249588798448	22.337874123121477
36	23.535630438755568	35.53034456581334	22.641530653845614	18.29249434158548
37	22.99157915454149	27.383633475320877	27.83715012722646	21.787637242911167
38	19.57010107826185	33.098843012385245	23.517354814221246	23.813701095131652
39	25.382593170539693	26.417274682636748	25.45316519758762	22.74696694923594
40	21.262142746685786	31.388244555972616	26.6205558601493	20.729056837192303
41	25.189433877384616	27.72749638002053	23.918012736704483	23.165057005890375
42	19.761292227236304	28.717472902872082	29.291327513249826	22.229907356641785
43	25.988781578170467	26.02364584651287	24.690649909324787	23.296922665991872
44	23.15859024643977	25.69496576834943	27.61728030590584	23.529163679304965
45	20.409936316477584	25.77087990103046	26.519337016574585	27.29984676591737
46	26.060197095581515	29.34249926194593	24.448568175108598	20.148735467363952
47	18.928204911925548	27.0760406562355	34.12986939957544	19.865885032263506
48	22.215286857014323	27.801723531975313	26.691971377560346	23.291018233450018
49	21.071795088074452	24.439852108023	32.7288319064288	21.759520897473745
50	22.541717627542774	24.27537148721409	28.98007956925759	24.20283131598555
51	21.322874052830613	23.415011316829037	26.842112662196165	28.42000196814418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	198.0
1	458.0
2	718.0
3	2598.5
4	4479.0
5	3015.0
6	1551.0
7	1499.0
8	1447.0
9	1382.0
10	1317.0
11	1257.0
12	1197.0
13	1145.5
14	1094.0
15	1097.0
16	1100.0
17	993.0
18	886.0
19	989.0
20	1092.0
21	1539.0
22	1986.0
23	1681.0
24	1376.0
25	2116.5
26	3652.0
27	4447.0
28	5318.0
29	6189.0
30	7135.0
31	8081.0
32	8294.0
33	8507.0
34	9449.5
35	10392.0
36	10391.5
37	10391.0
38	11109.5
39	11828.0
40	13220.5
41	14613.0
42	15897.0
43	17181.0
44	18508.0
45	19835.0
46	38669.0
47	57503.0
48	44860.0
49	32217.0
50	30857.0
51	29497.0
52	25165.0
53	20833.0
54	19007.0
55	17181.0
56	15766.5
57	14352.0
58	13169.5
59	11987.0
60	11173.0
61	10359.0
62	9838.0
63	9317.0
64	7764.5
65	6212.0
66	5216.5
67	4221.0
68	3653.5
69	3086.0
70	2739.0
71	2392.0
72	1993.0
73	1594.0
74	1256.0
75	784.0
76	650.0
77	456.5
78	263.0
79	202.0
80	141.0
81	129.0
82	117.0
83	79.5
84	42.0
85	27.5
86	13.0
87	9.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	355665.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.69898327390219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5316113072693	21.102824830103987
2	10.32222506235978	5.924747288282346
3	3.4774469257817993	2.993975734766834
4	1.7048215576212715	1.9570658146864304
5	1.1021892012382541	1.5815854725506135
6	0.8153971546065217	1.4040641580984004
7	0.6984833765417628	1.4032033918329543
8	0.5146193557451635	1.1815241826365415
9	0.5036274849304976	1.3008237089668002
>10	7.10974117973287	41.91084217069088
>50	0.13290169738628022	2.6549880721482717
>100	0.06994826178225275	3.882396686189598
>500	0.006994826178225275	1.2064738409513693
>1k	0.006994826178225275	4.076831975983995
>5k	0.002997782647810832	7.4186526721109995
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC	9394	2.641249490391239	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG	9047	2.5436857717233914	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC	7428	2.088482139091561	No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	3270	0.9194044958036355	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2697	0.7582978364472186	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT	2315	0.650893396876274	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT	1885	0.5299931114953679	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTA	1531	0.43046124864690083	No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT	1311	0.36860528868457676	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC	1207	0.3393642894296599	No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT	711	0.19990721606005651	No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC	695	0.1954086007900693	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	639	0.17966344734511408	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	618	0.17375901480325587	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	522	0.14676732318333263	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	517	0.14536150591146163	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	505	0.14198754445897122	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCC	467	0.1313033331927516	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATG	448	0.1259612275596418	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	447	0.1256800641052676	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	433	0.12174377574402878	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGACAGAG	430	0.12090028538090619	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	377	0.10599862229907356	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20974793696315353	0.0
2	0.0	0.0	0.0	1.562425315957432	0.0
3	0.0	0.0	0.0	1.8652383563184458	0.0
4	0.0	0.0	0.0	2.6350638943950067	0.0
5	0.0	0.0	0.0	5.834704005173408	0.0
6	0.0	0.0	0.0	6.515400728213347	0.0
7	0.0	0.0	0.0	7.599285844825889	0.0
8	0.0	0.0	0.0	8.643245750917297	0.0
9	0.0	0.0	0.0	8.937061560738336	0.0
10	0.0	0.0	0.0	12.234546553638959	0.0
11	0.0	0.0	0.0	13.364823640223245	0.0
12	0.0	0.0	0.0	16.97327541366173	0.0
13	0.0	0.0	0.0	17.461937497364094	0.0
14	0.0	0.0	0.0	17.71217297175713	0.0
15	0.0	0.0	0.0	18.5146134705411	0.0
16	0.0	0.0	0.0	19.190530414856678	0.0
17	0.0	0.0	0.0	19.863354561174138	0.0
18	0.0	0.0	0.0	20.46448202662618	0.0
19	0.0	0.0	0.0	21.66139485189715	0.0
20	0.0	0.0	0.0	22.230188520096156	0.0
21	0.0	0.0	0.0	22.755964179775912	0.0
22	0.0	0.0	0.0	23.321102723068055	0.0
23	0.0	0.0	0.0	23.80386037422856	0.0
24	0.0	0.0	0.0	24.235446276692954	0.0
25	0.0	0.0	0.0	24.667032179157353	0.0
26	0.0	0.0	0.0	25.052507275104382	0.0
27	0.0	0.0	0.0	25.533296782084264	0.0
28	0.0	0.0	0.0	25.92186467602941	0.0
29	0.0	0.0	0.0	26.30818326233956	0.0
30	2.8116345437420045E-4	0.0	0.0	26.714745617364656	0.0
31	2.8116345437420045E-4	0.0	0.0	27.121870299298497	0.0
32	2.8116345437420045E-4	0.0	0.0	27.50622074142803	0.0
33	2.8116345437420045E-4	0.0	0.0	27.87257672247761	0.0
34	2.8116345437420045E-4	0.0	0.0	28.253553203154652	0.0
35	2.8116345437420045E-4	0.0	0.0	28.658990904362252	0.0
36	2.8116345437420045E-4	0.0	0.0	29.069489547748585	0.0
37	2.8116345437420045E-4	0.0	0.0	29.463118383872466	0.0
38	2.8116345437420045E-4	0.0	0.0	29.846906499093247	0.0
39	2.8116345437420045E-4	0.0	0.0	30.229569960496534	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	25	3.885354E-5	45.000004	5
GCGAACC	30	2.1612595E-6	45.000004	33
GGATACA	30	2.1612595E-6	45.000004	8
TCGATGG	25	3.885354E-5	45.000004	2
ACGATAG	30	2.1612595E-6	45.000004	1
CGGCGAA	40	6.7921064E-9	45.0	31
ATTACGG	20	7.026711E-4	45.0	2
CGCGCAG	20	7.026711E-4	45.0	41
TAACCGC	20	7.026711E-4	45.0	37
AAGCATG	20	7.026711E-4	45.0	3
CGAATAC	20	7.026711E-4	45.0	45
GACGCGT	20	7.026711E-4	45.0	45
CGATCGA	35	1.208773E-7	45.0	41
ACGACGA	20	7.026711E-4	45.0	38
GCATATC	55	1.8189894E-12	45.0	28
CGATGAA	950	0.0	43.81579	19
GATACCT	980	0.0	43.622448	5
GATGAAT	990	0.0	43.181816	20
TGATACC	985	0.0	43.172592	4
TACGGCT	1140	0.0	43.026318	7
>>END_MODULE