SRR3551783_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833734
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	19008	2.2798638414650236	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	18934	2.2709881089172326	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	18497	2.218573309952575	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	6793	0.8147682594208705	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5118	0.6138648537783035	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	4303	0.5161118534208752	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTTACCCT	4230	0.5073560632048111	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3914	0.4694542863791089	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	3264	0.3914917707566202	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTA	3214	0.3854946541702749	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	2177	0.2611144561694737	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	1866	0.22381239100240602	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	1122	0.1345752961975882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	942	0.11298567648674518	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATG	922	0.11058682985220707	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTTACCC	847	0.10159115497268914	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	844	0.10123132797750842	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCACG	30	2.164781E-6	45.000004	16
GCGAACC	60	0.0	45.000004	33
ACCGCTA	30	2.164781E-6	45.000004	10
TAGCCGT	35	1.2113924E-7	45.000004	44
AATCGCG	30	2.164781E-6	45.000004	1
GAATCGT	20	7.0321705E-4	45.0	9
CATTAGT	20	7.0321705E-4	45.0	27
CGCTATT	55	1.8189894E-12	45.0	43
CGTTTTT	2945	0.0	42.93718	1
CGATGAA	1475	0.0	42.55932	19
CGATCCC	90	0.0	42.5	34
TACGGCT	2185	0.0	42.21968	7
TGATACC	2030	0.0	42.00739	4
TAATACG	75	0.0	42.0	4
GATACCT	2030	0.0	41.89655	5
ACGGCTG	2235	0.0	41.37584	8
CCGATGA	1515	0.0	41.28713	18
ACCACCG	1975	0.0	41.012657	14
CGACAGG	55	6.002665E-11	40.909092	2
CCACCGA	1880	0.0	40.81117	15

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content