##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	833734
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16428261291971	31.0	31.0	33.0	28.0	34.0
2	31.07020224675976	31.0	31.0	34.0	28.0	34.0
3	30.950840435918412	31.0	30.0	34.0	27.0	34.0
4	34.872727992381265	35.0	35.0	37.0	32.0	37.0
5	34.55439864513142	35.0	35.0	37.0	32.0	37.0
6	34.77024206761389	35.0	35.0	37.0	32.0	37.0
7	35.296874062950536	36.0	35.0	37.0	33.0	37.0
8	34.77242022035805	37.0	35.0	37.0	32.0	37.0
9	36.63064238714026	39.0	37.0	39.0	32.0	39.0
10	36.24390273156666	37.0	35.0	39.0	32.0	39.0
11	36.55164476919497	38.0	35.0	39.0	32.0	39.0
12	36.650903045815575	39.0	35.0	39.0	32.0	39.0
13	36.620526450882416	39.0	35.0	39.0	32.0	39.0
14	37.50461058323158	39.0	36.0	41.0	32.0	41.0
15	37.51631575538481	39.0	36.0	41.0	32.0	41.0
16	37.776196004960816	39.0	36.0	41.0	33.0	41.0
17	37.493102116502385	39.0	36.0	41.0	32.0	41.0
18	37.50315808159437	39.0	36.0	40.0	33.0	41.0
19	37.45728733624873	39.0	36.0	40.0	32.0	41.0
20	37.239605197820886	39.0	35.0	40.0	32.0	41.0
21	37.271808514466244	39.0	35.0	40.0	32.0	41.0
22	37.42880702958018	39.0	35.0	41.0	32.0	41.0
23	37.4152091674323	39.0	35.0	41.0	32.0	41.0
24	37.31341890818894	39.0	35.0	41.0	32.0	41.0
25	37.06689663609736	39.0	35.0	40.0	32.0	41.0
26	37.10862457330516	39.0	35.0	40.0	32.0	41.0
27	37.11642802140731	39.0	35.0	40.0	32.0	41.0
28	36.94821729712354	39.0	35.0	41.0	31.0	41.0
29	37.08003152084478	39.0	35.0	41.0	31.0	41.0
30	36.65939616232515	39.0	35.0	40.0	30.0	41.0
31	36.70220837821176	39.0	35.0	40.0	30.0	41.0
32	36.5652390330729	39.0	35.0	40.0	30.0	41.0
33	36.38925604569323	39.0	35.0	41.0	30.0	41.0
34	36.33878431250255	39.0	35.0	41.0	30.0	41.0
35	36.212481438924165	39.0	35.0	41.0	30.0	41.0
36	36.13769739509244	39.0	35.0	41.0	29.0	41.0
37	36.2929195642735	39.0	35.0	41.0	30.0	41.0
38	35.94830605444902	39.0	35.0	40.0	28.0	41.0
39	35.981001134654456	39.0	35.0	40.0	29.0	41.0
40	35.85856760069758	39.0	35.0	40.0	28.0	41.0
41	35.79986062701053	39.0	35.0	40.0	27.0	41.0
42	35.973146111349664	39.0	35.0	40.0	29.0	41.0
43	35.97841157971247	39.0	35.0	40.0	29.0	41.0
44	36.0919585863117	39.0	35.0	40.0	30.0	41.0
45	36.13958888566377	39.0	35.0	40.0	30.0	41.0
46	36.02280583495455	39.0	35.0	40.0	30.0	41.0
47	35.78659500512154	38.0	35.0	40.0	29.0	41.0
48	35.80866079588934	38.0	35.0	40.0	29.0	41.0
49	35.94084804026224	39.0	35.0	40.0	29.0	41.0
50	35.834216908510385	39.0	35.0	40.0	29.0	41.0
51	34.930699719574825	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	21.0
10	29.0
11	33.0
12	24.0
13	26.0
14	34.0
15	47.0
16	84.0
17	117.0
18	285.0
19	510.0
20	1083.0
21	2305.0
22	4182.0
23	5876.0
24	7080.0
25	8493.0
26	9567.0
27	10078.0
28	10723.0
29	12379.0
30	15608.0
31	19725.0
32	27212.0
33	37901.0
34	59212.0
35	60179.0
36	71193.0
37	107307.0
38	182158.0
39	180230.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.00691827369401	18.191053741361156	19.69752942785109	14.10449855709375
2	29.048713378607566	32.96830883711112	21.562512743872748	16.420465040408573
3	27.950161562320837	32.02544216740591	25.62423986547268	14.40015640480057
4	25.951802373418857	21.127122079704076	35.949115665188174	16.971959881688882
5	31.321020853173792	25.617882921891155	24.318547642293588	18.742548582641465
6	23.710440020438174	37.05642327169097	25.36564419826947	13.867492509601384
7	69.19772973154508	5.030861161953333	20.8013587067338	4.970050399767792
8	67.72159945498204	13.284452835076896	12.957010269462444	6.036937440478618
9	62.43142297183514	7.016506463692257	13.82971067510741	16.722359889365194
10	40.05210294890217	24.699964257185144	20.979712954011713	14.268219839900976
11	30.31902261392722	21.776249979010093	29.262330671413185	18.6423967356495
12	24.87088207989599	19.47311732519005	31.9242108394284	23.731789755485565
13	25.48114866372248	20.260778617640636	36.425046837480544	17.83302588115634
14	19.621246104872778	27.950041619989108	30.755012989754526	21.673699285383588
15	16.947851473011777	23.76765251267191	38.7608038055303	20.523692208786017
16	20.474995622104892	26.233666852977088	27.823502460017224	25.467835064900797
17	20.86384866156352	27.581218949928875	31.164136283275003	20.390796105232603
18	20.54408240517959	25.045877941885543	31.604444583044472	22.805595069890398
19	19.99006877493301	26.821024451443748	28.714913869411586	24.473992904211656
20	22.7159981480904	27.75621481191843	33.6017242909609	15.926062749030267
21	21.08046451266231	30.957835472704726	31.20347736808143	16.758222646551538
22	19.858611979360326	21.920420661745833	34.31346208742836	23.907505271465478
23	20.935934002931393	27.46703385012486	32.39870270374004	19.198329443203708
24	22.007138967584385	25.224352131495177	29.052551533222825	23.71595736769761
25	19.239229778322585	32.79199360947256	26.941086725502377	21.027689886702476
26	18.74122921699247	24.103491041507244	32.6240743450549	24.531205396445387
27	22.561152597830965	26.36308462891042	28.17757222327505	22.898190549983568
28	16.031492058618216	26.768849537142543	33.6248731609842	23.574785243255043
29	20.461442138619752	23.132078096851032	33.57617657430308	22.83030319022614
30	19.446130300551495	27.469912466086306	30.911297847994685	22.172659385367517
31	20.65071113808481	22.611888204151445	31.114360215608333	25.62304044215541
32	22.770811793689592	25.804992959385125	30.399024149189067	21.02517109773621
33	20.60345385938441	25.058351944385137	26.69520494546222	27.64298925076823
34	19.1238452552013	22.22435453034181	33.194040305421154	25.457759909035737
35	18.360652198422997	24.164181861361058	27.870160027058986	29.60500591315696
36	18.32418972957802	25.97723014774496	30.300431552509554	25.39814857016746
37	17.2658185944198	23.697246363948214	32.75385194798341	26.283083093648575
38	18.16994389097722	26.589295866547364	28.58765505544934	26.65310518702608
39	20.423180534798867	23.35361158355063	30.491979456277424	25.731228425373082
40	19.184416132723385	24.542240090964267	29.48194508080515	26.7913986955072
41	17.735632707794093	24.16178301472652	26.354448781026086	31.748135496453305
42	18.75094454586235	21.57906478565106	32.60236478301233	27.06762588547426
43	20.898991764759504	22.56019305917715	28.420095618026853	28.120719558036495
44	20.617247227533003	21.97727332698439	30.729105446101514	26.6763739993811
45	19.03148965977158	20.594218299841437	29.87031835093687	30.503973689450113
46	23.888314498389175	24.345294782268688	28.17517337664051	23.59121734270163
47	16.1878968591901	23.752659721206044	36.57389527115363	23.485548148450224
48	19.67653951979888	24.565748787982738	28.623157985640503	27.13455370657788
49	19.82310904916916	20.600815128086417	34.995094358632365	24.580981464112057
50	19.966200250919357	21.793161847783583	30.565863932621195	27.674773968675858
51	19.05128014450652	20.729153423034205	28.70819709883488	31.511369333624394
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	203.0
1	550.0
2	897.0
3	4824.0
4	8751.0
5	5870.5
6	2990.0
7	2909.5
8	2829.0
9	2760.5
10	2692.0
11	2678.5
12	2665.0
13	2579.5
14	2494.0
15	2409.0
16	2324.0
17	2272.5
18	2221.0
19	2411.5
20	2602.0
21	2849.0
22	3096.0
23	3575.0
24	4054.0
25	4799.0
26	6849.5
27	8155.0
28	9175.5
29	10196.0
30	11687.0
31	13178.0
32	15485.5
33	17793.0
34	19769.0
35	21745.0
36	24171.5
37	26598.0
38	28125.0
39	29652.0
40	31856.0
41	34060.0
42	37504.5
43	40949.0
44	44971.0
45	48993.0
46	92055.0
47	135117.0
48	108615.5
49	82114.0
50	80859.5
51	79605.0
52	66862.5
53	54120.0
54	48500.5
55	42881.0
56	39090.0
57	35299.0
58	31948.0
59	28597.0
60	26023.0
61	23449.0
62	21102.0
63	18755.0
64	16128.5
65	13502.0
66	10912.5
67	8323.0
68	7022.5
69	5722.0
70	4755.0
71	3788.0
72	3386.5
73	2985.0
74	2521.0
75	1642.5
76	1228.0
77	955.5
78	683.0
79	533.0
80	383.0
81	349.5
82	316.0
83	204.5
84	93.0
85	51.5
86	10.0
87	10.5
88	11.0
89	11.5
90	12.0
91	7.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	833734.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.34150785884582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.76802433722271	23.857691388338186
2	9.861820451551404	6.378922872727524
3	3.480659774902519	3.3770935639193507
4	1.8291999285411535	2.3663633545725573
5	1.2668552622125968	2.048600470943444
6	0.9423807797365147	1.8286809236324246
7	0.7432077829341822	1.6825522246764864
8	0.6711895692982858	1.7365826184188682
9	0.6012295799123094	1.7500204065334077
>10	6.7218446242678755	38.89474482652586
>50	0.06597845627655219	1.4774436229421655
>100	0.03898726871618471	2.4473721023363635
>500	0.0037487758380946755	0.946407739563937
>1k	0.0029990206704757407	2.920697213181558
>5k	7.497551676189352E-4	1.4441023041181211
>10k+	0.0011246327514284028	6.842724367569695
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCG	19008	2.2798638414650236	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGC	18934	2.2709881089172326	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTC	18497	2.218573309952575	No Hit
GCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	6793	0.8147682594208705	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5118	0.6138648537783035	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCT	4303	0.5161118534208752	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTTACCCT	4230	0.5073560632048111	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCT	3914	0.4694542863791089	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTC	3264	0.3914917707566202	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTTACCCTCGTA	3214	0.3854946541702749	No Hit
CCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGC	2177	0.2611144561694737	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTGCT	1866	0.22381239100240602	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCC	1122	0.1345752961975882	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTT	942	0.11298567648674518	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATG	922	0.11058682985220707	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTTACCC	847	0.10159115497268914	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTTACCCTCGTATGCCGTCTTCTG	844	0.10123132797750842	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1994233172690571E-4	0.0	0.0	0.23040921924738586	0.0
2	1.1994233172690571E-4	0.0	0.0	1.521228593292345	0.0
3	1.1994233172690571E-4	0.0	0.0	1.9007261308762746	0.0
4	1.1994233172690571E-4	0.0	0.0	2.832438163730878	0.0
5	1.1994233172690571E-4	0.0	0.0	6.3829710675107405	0.0
6	1.1994233172690571E-4	0.0	0.0	7.281818901472172	0.0
7	1.1994233172690571E-4	0.0	0.0	8.592548702583798	0.0
8	1.1994233172690571E-4	0.0	0.0	10.074076384074537	0.0
9	1.1994233172690571E-4	0.0	0.0	10.529497417641599	0.0
10	1.1994233172690571E-4	0.0	0.0	13.77657622215239	0.0
11	1.1994233172690571E-4	0.0	0.0	15.127126877397348	0.0
12	1.1994233172690571E-4	0.0	0.0	18.547642293585245	0.0
13	1.1994233172690571E-4	0.0	0.0	19.09661834589929	0.0
14	1.1994233172690571E-4	0.0	0.0	19.388078211995673	0.0
15	1.1994233172690571E-4	0.0	0.0	20.11912672387116	0.0
16	1.1994233172690571E-4	0.0	0.0	20.802198303055892	0.0
17	1.1994233172690571E-4	0.0	0.0	21.544401451781983	0.0
18	1.1994233172690571E-4	0.0	0.0	22.230831416255064	0.0
19	1.1994233172690571E-4	0.0	0.0	23.351092794584364	0.0
20	1.1994233172690571E-4	0.0	0.0	23.980190324491986	0.0
21	1.1994233172690571E-4	0.0	0.0	24.619003183269484	0.0
22	1.1994233172690571E-4	0.0	0.0	25.36912252588955	0.0
23	2.3988466345381142E-4	0.0	0.0	25.97974893671123	0.0
24	2.3988466345381142E-4	0.0	0.0	26.493461943497568	0.0
25	2.3988466345381142E-4	0.0	0.0	26.944804937785914	0.0
26	2.3988466345381142E-4	0.0	0.0	27.377316985993133	0.0
27	2.3988466345381142E-4	0.0	0.0	27.9116600738365	0.0
28	2.3988466345381142E-4	0.0	0.0	28.348609988317616	0.0
29	2.3988466345381142E-4	0.0	0.0	28.832817181499134	0.0
30	2.3988466345381142E-4	0.0	0.0	29.386830811745714	0.0
31	2.3988466345381142E-4	0.0	0.0	29.868879042956145	0.0
32	2.3988466345381142E-4	0.0	0.0	30.326578980826017	0.0
33	2.3988466345381142E-4	0.0	0.0	30.778521686773	0.0
34	2.3988466345381142E-4	0.0	0.0	31.22890514240753	0.0
35	2.3988466345381142E-4	0.0	0.0	31.697279947801096	0.0
36	2.3988466345381142E-4	0.0	0.0	32.1345896892774	0.0
37	2.3988466345381142E-4	0.0	0.0	32.60056564803642	0.0
38	2.3988466345381142E-4	0.0	0.0	33.10480321061634	0.0
39	2.3988466345381142E-4	0.0	0.0	33.86103961215448	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCACG	30	2.164781E-6	45.000004	16
GCGAACC	60	0.0	45.000004	33
ACCGCTA	30	2.164781E-6	45.000004	10
TAGCCGT	35	1.2113924E-7	45.000004	44
AATCGCG	30	2.164781E-6	45.000004	1
GAATCGT	20	7.0321705E-4	45.0	9
CATTAGT	20	7.0321705E-4	45.0	27
CGCTATT	55	1.8189894E-12	45.0	43
CGTTTTT	2945	0.0	42.93718	1
CGATGAA	1475	0.0	42.55932	19
CGATCCC	90	0.0	42.5	34
TACGGCT	2185	0.0	42.21968	7
TGATACC	2030	0.0	42.00739	4
TAATACG	75	0.0	42.0	4
GATACCT	2030	0.0	41.89655	5
ACGGCTG	2235	0.0	41.37584	8
CCGATGA	1515	0.0	41.28713	18
ACCACCG	1975	0.0	41.012657	14
CGACAGG	55	6.002665E-11	40.909092	2
CCACCGA	1880	0.0	40.81117	15
>>END_MODULE