FastQCFastQC Report
Sat 18 Jun 2016
SRR3551782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370925
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC65311.7607333018804339No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG64331.7343128664824423No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC63141.7022309092134529No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31560.8508458583271551No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC22060.5947293927343803No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT15150.408438363550583No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCAT14950.4030464379591561No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT13370.3604502257868842No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC12350.33295140527060724No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTA11980.3229763429264676No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC11090.2989822740446182No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC7810.21055469434521804No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT5630.1517827053986655No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC4420.11916155557053312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG302.1615124E-645.0000044
CTAGACG253.88568E-545.016
ATAGGCG207.027102E-445.01
TAGGTCG207.027102E-445.01
AGGCGAT207.027102E-445.01
GACCCCG207.027102E-445.09
TGCGCAT207.027102E-445.020
TATTAGC207.027102E-445.018
CAGTGCG207.027102E-445.01
GCTACGA207.027102E-445.010
CGCAATG207.027102E-445.041
ACGGTAT253.88568E-545.016
TACGGCT7350.044.0816357
ACGGCTG7650.042.3529438
CCGATGA6550.042.25190718
GATGAAT7200.042.187520
CGTTTTT18100.042.1408841
TTGTAGG750.042.02
TGATACC8350.041.7664684
GATACCT8500.041.5588235
Produced by FastQC (version 0.11.5)