##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370925
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114187504212442	31.0	31.0	33.0	28.0	34.0
2	31.028469367122735	31.0	31.0	34.0	28.0	34.0
3	30.92935229493833	31.0	30.0	34.0	27.0	34.0
4	34.78008761879086	35.0	35.0	37.0	32.0	37.0
5	34.55728786142751	35.0	35.0	37.0	32.0	37.0
6	34.745600862708095	35.0	35.0	37.0	32.0	37.0
7	35.21379524162567	36.0	35.0	37.0	32.0	37.0
8	34.6203787827728	36.0	35.0	37.0	31.0	37.0
9	36.37984498213925	39.0	35.0	39.0	32.0	39.0
10	36.169166273505425	37.0	35.0	39.0	32.0	39.0
11	36.521771247556785	38.0	35.0	39.0	32.0	39.0
12	36.61136078722114	39.0	35.0	39.0	32.0	39.0
13	36.63137022309092	39.0	35.0	39.0	32.0	39.0
14	37.55714227943654	39.0	36.0	41.0	32.0	41.0
15	37.628574509671765	39.0	36.0	41.0	32.0	41.0
16	37.783996764844645	39.0	36.0	41.0	33.0	41.0
17	37.51810743411741	39.0	36.0	41.0	32.0	41.0
18	37.52479072588798	39.0	36.0	40.0	33.0	41.0
19	37.42354384309497	39.0	36.0	40.0	32.0	41.0
20	37.2280487969266	39.0	35.0	40.0	32.0	41.0
21	37.180206241153876	39.0	35.0	40.0	32.0	41.0
22	37.28662667655186	39.0	35.0	40.0	32.0	41.0
23	37.31669474961245	39.0	35.0	41.0	32.0	41.0
24	37.22322302352227	39.0	35.0	41.0	32.0	41.0
25	36.93082429062479	39.0	35.0	40.0	32.0	41.0
26	37.014827795376426	39.0	35.0	40.0	32.0	41.0
27	37.02444968659432	39.0	35.0	40.0	32.0	41.0
28	36.87634697041181	39.0	35.0	41.0	31.0	41.0
29	36.95058839388017	39.0	35.0	41.0	31.0	41.0
30	36.5898092606322	39.0	35.0	40.0	30.0	41.0
31	36.63903754128193	39.0	35.0	40.0	31.0	41.0
32	36.44095976275528	39.0	35.0	40.0	30.0	41.0
33	36.34528813102379	39.0	35.0	41.0	30.0	41.0
34	36.20534339826111	39.0	35.0	41.0	30.0	41.0
35	36.100510884949784	39.0	35.0	41.0	29.0	41.0
36	35.96757565545595	39.0	35.0	41.0	29.0	41.0
37	36.012045561771245	39.0	35.0	41.0	29.0	41.0
38	35.82812967581047	39.0	35.0	40.0	28.0	41.0
39	35.745530767675405	39.0	35.0	40.0	28.0	41.0
40	35.71523892970277	39.0	35.0	40.0	27.0	41.0
41	35.50130349801173	38.0	35.0	40.0	26.0	41.0
42	35.57878816472333	39.0	35.0	40.0	27.0	41.0
43	35.58146795174227	39.0	35.0	40.0	27.0	41.0
44	35.69941093212913	39.0	35.0	40.0	27.0	41.0
45	35.702306396171736	39.0	35.0	40.0	27.0	41.0
46	35.56257194850711	38.0	35.0	40.0	27.0	41.0
47	35.36772123744692	38.0	35.0	40.0	26.0	41.0
48	35.41454741524567	38.0	35.0	40.0	27.0	41.0
49	35.42955044820381	38.0	35.0	40.0	27.0	41.0
50	35.35456493900384	38.0	35.0	40.0	27.0	41.0
51	34.49226932668329	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	26.0
10	26.0
11	24.0
12	23.0
13	14.0
14	27.0
15	33.0
16	54.0
17	99.0
18	180.0
19	341.0
20	606.0
21	1249.0
22	1891.0
23	2656.0
24	3271.0
25	4333.0
26	4921.0
27	5282.0
28	5430.0
29	5941.0
30	7134.0
31	8854.0
32	11707.0
33	16295.0
34	26260.0
35	28851.0
36	31635.0
37	47472.0
38	79689.0
39	76583.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.705533463638204	19.284221877738087	19.49747253487902	16.512772123744693
2	32.45669609759385	31.9040237244726	19.57268989687942	16.066590281054122
3	29.450158387814245	29.2493091595336	25.59331401226663	15.707218440385523
4	26.72804475298241	21.496259351620946	33.84619532250455	17.929500572892092
5	29.93138774684909	26.021702500505494	24.7958482172946	19.25106153535081
6	23.973040372042867	35.43789175709375	26.85637258205837	13.732695288805013
7	66.4449686594325	6.438228752443216	21.304306800566152	5.812495787558132
8	64.60524364763766	12.991844712542965	15.469973714362743	6.932937925456628
9	58.32553750758239	8.246950192087349	17.03848486890881	16.389027431421447
10	40.97674732088697	19.52551054795444	24.392262586776305	15.10547954438229
11	31.41713284356676	21.750758239536296	27.634697041180832	19.197411875716114
12	25.865067062074544	19.31064231313608	32.129136617914675	22.695154006874706
13	25.594122801105346	21.27761676888859	35.48183595066388	17.646424479342183
14	19.72312462088023	27.895396643526322	30.86877401091865	21.5127047246748
15	17.267641706544453	23.093347711801577	39.47967918042731	20.159331401226662
16	19.907528476107032	24.467210352497133	31.24863516883467	24.376626002561164
17	19.975466738559007	26.683022174293995	31.75601536698793	21.585495720159063
18	20.156096245871808	25.573903080137494	31.785940554020353	22.484060119970344
19	21.275999191211163	25.86884140998854	29.689559884073603	23.165599514726697
20	23.307676754060793	26.368672912313812	33.37574981465256	16.94790051897284
21	22.189930578958013	28.78964750286446	30.8946552537575	18.12576666442003
22	19.623643593718405	22.898160005391926	33.89957538585968	23.578621015029995
23	21.380332951405272	26.755543573498684	31.63065309698726	20.23347037810878
24	22.020624115387207	24.403855226797873	30.09934622902204	23.47617442879288
25	19.357821662061063	29.825975601536697	28.551324391723394	22.26487834467884
26	19.54141672844915	24.676956258003642	32.303835007076906	23.47779200647031
27	23.640897755610972	25.611376962997912	28.415447866819438	22.33227741457168
28	17.35337332344814	25.847812900181978	32.561838646626676	24.23697512974321
29	23.70640965154681	21.679045629170318	31.712071173417804	22.902473545865067
30	20.11808317045225	25.71382354923502	31.176383365909548	22.99170991440318
31	21.881242838848824	22.933477118015773	28.590955044820383	26.594324998315024
32	23.85576599042933	24.823347037810876	30.121183527667316	21.19970344409247
33	21.25227471860888	23.157242030059987	30.0489317247422	25.541551526588936
34	21.995282065107503	22.70998180225113	31.688616297095102	23.60611983554627
35	19.93610568174159	23.756284963267507	28.898833996090858	27.408775358900044
36	21.342859068544854	28.2709442609692	27.475635236233742	22.91056143425221
37	19.354316910426636	24.040169845656127	32.40870795983015	24.19680528408708
38	19.31441666105008	24.41760463705601	28.520860012131834	27.74711868976208
39	21.333962391319	24.32594190200175	33.85077845925726	20.489317247421983
40	20.091393138774684	21.71544112691245	33.76639482375143	24.426770910561434
41	18.24654579766799	26.410190739367795	29.58226056480421	25.761002898160008
42	19.571072319201996	23.669205364965965	30.91190941565006	25.847812900181978
43	22.253016108377704	25.98368942508593	27.69481701152524	24.06847745501112
44	20.69501920873492	22.52099481027162	30.98254364089776	25.801442340095708
45	20.44995619060457	21.10453595740379	29.453393543169103	28.992114308822536
46	23.851991642515333	24.9360382826717	28.965424277144976	22.24654579766799
47	17.261171395834737	23.95362943991373	35.679719619869246	23.105479544382288
48	20.02399406888185	23.727168565073804	30.424748938464653	25.8240884275797
49	19.82907595875177	20.80204893172474	35.4769832176316	23.89189189189189
50	19.605580642987128	21.663678641234753	32.87942306396172	25.8513176518164
51	19.52011862236301	21.31994338478129	30.00525712745164	29.15468086540406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	168.0
1	446.0
2	724.0
3	3165.0
4	5606.0
5	3752.5
6	1899.0
7	1849.0
8	1799.0
9	1768.5
10	1738.0
11	1687.0
12	1636.0
13	1667.5
14	1699.0
15	1603.0
16	1507.0
17	1462.5
18	1418.0
19	1334.5
20	1251.0
21	1429.5
22	1608.0
23	1821.5
24	2035.0
25	2368.0
26	2964.5
27	3228.0
28	4021.0
29	4814.0
30	5288.0
31	5762.0
32	6174.5
33	6587.0
34	7351.5
35	8116.0
36	8802.0
37	9488.0
38	10641.5
39	11795.0
40	13207.0
41	14619.0
42	16201.0
43	17783.0
44	19609.5
45	21436.0
46	36174.5
47	50913.0
48	43373.5
49	35834.0
50	35815.5
51	35797.0
52	31063.5
53	26330.0
54	23863.5
55	21397.0
56	19407.0
57	17417.0
58	15787.0
59	14157.0
60	13047.5
61	11938.0
62	10538.5
63	9139.0
64	7710.0
65	6281.0
66	5050.0
67	3819.0
68	3310.0
69	2801.0
70	2436.5
71	2072.0
72	1736.5
73	1401.0
74	1243.5
75	799.0
76	512.0
77	423.5
78	335.0
79	255.5
80	176.0
81	123.0
82	70.0
83	39.5
84	9.0
85	15.5
86	22.0
87	11.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370925.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.77792616989149
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.43940679762395	26.236261199081756
2	9.54223800533092	6.637184976898627
3	3.246139466733305	3.386819961336654
4	1.6288877651706124	2.2659735374457237
5	1.096100914703395	1.9060058343152597
6	0.8250509318764963	1.721613623712057
7	0.7152368069709945	1.7412117006778323
8	0.6398381889695899	1.7801796237329182
9	0.5613940122257106	1.7571707558464427
>10	6.188460293172863	39.48441996164861
>50	0.06677633298747349	1.5921547283533806
>100	0.040376387387774666	2.343945563451042
>500	0.001552937976452872	0.36293350659887097
>1k	0.006211751905811488	3.578297541623244
>5k	0.002329406964679308	5.205827485277556
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGC	6531	1.7607333018804339	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCG	6433	1.7343128664824423	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTC	6314	1.7022309092134529	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3156	0.8508458583271551	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	2206	0.5947293927343803	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCT	1515	0.408438363550583	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTTTCAT	1495	0.4030464379591561	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCT	1337	0.3604502257868842	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTC	1235	0.33295140527060724	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTTTCATCGTA	1198	0.3229763429264676	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	1109	0.2989822740446182	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCC	781	0.21055469434521804	No Hit
CTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGCT	563	0.1517827053986655	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTTTCATCGTATGCCGTCTTCTGC	442	0.11916155557053312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16067938262451978	0.0
2	0.0	0.0	0.0	1.3045763968457236	0.0
3	0.0	0.0	0.0	1.6987261575790253	0.0
4	0.0	0.0	0.0	2.6153535081215877	0.0
5	0.0	0.0	0.0	5.780144234009571	0.0
6	0.0	0.0	0.0	6.631529284895868	0.0
7	0.0	0.0	0.0	7.827458381074341	0.0
8	0.0	0.0	0.0	9.16735189054391	0.0
9	0.0	0.0	0.0	9.562310440115926	0.0
10	0.0	0.0	0.0	12.173620004043944	0.0
11	0.0	0.0	0.0	13.678776032890745	0.0
12	0.0	0.0	0.0	16.65107501516479	0.0
13	0.0	0.0	0.0	17.208869717597896	0.0
14	0.0	0.0	0.0	17.48709307811552	0.0
15	0.0	0.0	0.0	18.20394958549572	0.0
16	0.0	0.0	0.0	18.949922491069625	0.0
17	0.0	0.0	0.0	19.777852665633215	0.0
18	0.0	0.0	0.0	20.540810136820113	0.0
19	0.0	0.0	0.0	21.568241558266497	0.0
20	0.0	0.0	0.0	22.180764305452584	0.0
21	0.0	0.0	0.0	22.82294264339152	0.0
22	0.0	0.0	0.0	23.571341915481565	0.0
23	0.0	0.0	0.0	24.174159196603085	0.0
24	0.0	0.0	0.0	24.690705668261778	0.0
25	0.0	0.0	0.0	25.133921951877063	0.0
26	0.0	0.0	0.0	25.567971961986924	0.0
27	0.0	0.0	0.0	26.069690638269194	0.0
28	2.695962795713419E-4	0.0	0.0	26.50697580373391	0.0
29	2.695962795713419E-4	0.0	0.0	26.94318258408034	0.0
30	2.695962795713419E-4	0.0	0.0	27.4195592100829	0.0
31	2.695962795713419E-4	0.0	0.0	27.894318258408035	0.0
32	2.695962795713419E-4	0.0	0.0	28.31758441733504	0.0
33	2.695962795713419E-4	0.0	0.0	28.75406079396104	0.0
34	2.695962795713419E-4	0.0	0.0	29.21560962458718	0.0
35	2.695962795713419E-4	0.0	0.0	29.664487430073464	0.0
36	2.695962795713419E-4	0.0	0.0	30.138707285839455	0.0
37	2.695962795713419E-4	0.0	0.0	30.576262047583743	0.0
38	2.695962795713419E-4	0.0	0.0	31.016782368403316	0.0
39	2.695962795713419E-4	0.0	0.0	31.549504616836288	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1615124E-6	45.000004	4
CTAGACG	25	3.88568E-5	45.0	16
ATAGGCG	20	7.027102E-4	45.0	1
TAGGTCG	20	7.027102E-4	45.0	1
AGGCGAT	20	7.027102E-4	45.0	1
GACCCCG	20	7.027102E-4	45.0	9
TGCGCAT	20	7.027102E-4	45.0	20
TATTAGC	20	7.027102E-4	45.0	18
CAGTGCG	20	7.027102E-4	45.0	1
GCTACGA	20	7.027102E-4	45.0	10
CGCAATG	20	7.027102E-4	45.0	41
ACGGTAT	25	3.88568E-5	45.0	16
TACGGCT	735	0.0	44.081635	7
ACGGCTG	765	0.0	42.352943	8
CCGATGA	655	0.0	42.251907	18
GATGAAT	720	0.0	42.1875	20
CGTTTTT	1810	0.0	42.140884	1
TTGTAGG	75	0.0	42.0	2
TGATACC	835	0.0	41.766468	4
GATACCT	850	0.0	41.558823	5
>>END_MODULE