SRR3551781_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	240589
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGC	6317	2.625639576206726	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCG	5434	2.2586236278466596	No Hit
GAATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTC	4874	2.0258615314914645	No Hit
GCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	2361	0.9813416240975272	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2333	0.9697035192797675	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAAGCCACAT	1494	0.6209760213476094	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCT	1217	0.5058419129719147	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAAGCCACATCGTA	1014	0.4214656530431566	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTC	890	0.36992547456450625	No Hit
GAACTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCT	876	0.3641064221556264	No Hit
CCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	789	0.32794516790044437	No Hit
CTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGCT	659	0.2739111098179884	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCC	451	0.18745661688605877	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	321	0.13342255880360282	No Hit
TCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATGCCGTCTTCTGC	320	0.13300691220296856	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAAGCCACA	306	0.12718785979408867	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAAGCCACATCGTATG	295	0.12261574718711163	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	292	0.12136880738520879	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	265	0.11014634916808332	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	262	0.10889940936618049	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAAGCCACATCGTAT	253	0.105158589960472	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTCC	25	3.8815815E-5	45.000004	37
CGACAGG	25	3.8815815E-5	45.000004	2
ATCACCC	25	3.8815815E-5	45.000004	36
TAGCACG	25	3.8815815E-5	45.000004	1
TCGTCCC	25	3.8815815E-5	45.000004	38
AACTGCA	25	3.8815815E-5	45.000004	17
CACCGAG	50	2.1827873E-11	45.000004	16
GCACTAG	25	3.8815815E-5	45.000004	1
CTTCGGG	20	7.022156E-4	45.0	25
GATACGC	20	7.022156E-4	45.0	27
ACACGCG	35	1.206572E-7	45.0	36
CACGGTT	20	7.022156E-4	45.0	7
CGGTTTA	20	7.022156E-4	45.0	9
TCGGGTA	20	7.022156E-4	45.0	27
CGCCTAA	20	7.022156E-4	45.0	28
GTGCTCA	20	7.022156E-4	45.0	8
AGGCACG	20	7.022156E-4	45.0	1
TTGCACT	20	7.022156E-4	45.0	34
CGGGTGA	30	2.1583237E-6	44.999996	6
GATCACC	30	2.1583237E-6	44.999996	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content